MD16G1240100.v1.1

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002699
Physical Location & Seq
Reverse (-)
25557912 .. 25559123
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1240100.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGTGTCTCTTTGTCTCACTGGCAACTTTCTCAATGATCCATTGCCTGCAGCTGAGGCAGTAGCTTTGAACCAAGACCTCGTCACATTGATCCTGGTACGCCTGCCGGTGAAGTCACTTCTCCGCTTCAAATGTGTCTCCAAGCAGTGGCTCTGTCTCATCTCCAGCCAAAATTTCTATAGCCGCCACCGCAGTCTTTCCTCTGCATGCATCCTCTTGTCTCGAATTCCCAGCCTCATCAGTCACCTATCTCTCAATTGCAGCAGTTCAGAGCAACCCTTTTCGTCTCTTGACTTCATTGATGATTCAGCAGGTGTTAAGTTACTGCAGTCCATAAATGGCCTGGTCCTGGGGTGTAGCTTTTACAAGTTGGGAAAAACTATTAGTTATTATGTCTGCAACCCTTCAACAAGGCAATTCTCGATTCTTCGTCCACCTAATACCAAGGGTCATGATTCCAAGGGTCGTAATTCGTTCGCAACTATTTTAGGTGTTTATTTAGCTTTTGATCCATCCAAATCATCTCACTACCAAGTCGTCAGCGTTCAAAATTGCTCCTCATCAGAAGCAAGTGATCACTACCAAATTCAGATATACGCATCCGAGACTCGAACTTGGAGGGTTTCCCGATCTCCCTTTATGGCCCCATCCGATATGGTGTTTGAAAATGGCGTGCTTTGGAATGGTGCAATACATTGGATTAGCCCAACAGGAGCTACATTATGTTTTGATATCAATCAAGAGCGCTTCGGATCAATGCCTAGTCCTCCATCAGGCGAGCATTGGGGTACAAGAAGGTTCAAGTATTTTGGGGAATCTGGTGGGCATTTGCACCTAGTTGAAGTTTATGGTCCAAGCATCACTCAATTCCAAGTCTTTGAATTGGAGAGAGATTACTCAAGGTGGATTCCCAAGTACCAAATCGACATTTCTCCGATCATCGATGCATTTCCGGAGATGGTTAGAAACTACCTTCACCCCCATCCCTATGATTCTCCTTTTTACGCGTTTGTTTTACTTTTTGTCCTAGAAACTGAAGAGGGCTCATCATTGTTGCTGTACATTCCTGGTAAGTTTATATCTTATAATATTAGAGACAAGAGTTTCAAAGAGATTTGTGATTTTGATCCGAAATCCACCAAAACAAATACAGCATTACATGTTGGATGCTTTCATGCTCATCAGTTTATAGAGACTCTTGCCAGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

404

Amino Acids

45.42

Weight (kDa)

7.2

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 23 - 61 4.9e-07 F-box domain
FBA_3 PF08268 98 - 255 2.6e-11 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 102 - 302 2.6e-08 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 105 - 318 4.9e-07 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 109 - 261 1.2e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1086
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 147
AccII CGCG 1 cut(s) 1007
AccIII TCCGGA 1 cut(s) 952
AciI CCGC 3 cut(s) 124, 184, 190
AclWI GGATC 5 cut(s) 32, 85, 503, 760, 1121
AcsI RAATTY 3 cut(s) 172, 225, 585
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 4 cut(s) 99, 790, 917, 1061
AfeI AGCGCT 1 cut(s) 746
AfiI CCNNNNNNNGG 3 cut(s) 147, 349, 773
AflIII ACRYGT 2 cut(s) 1005, 1159
AgsI TTSAA 9 cut(s) 70, 130, 408, 548, 665, 802, 842, 881, 1108
AjnI CCWGG 4 cut(s) 93, 342, 348, 1066
AluBI AGCT 5 cut(s) 53, 65, 360, 503, 716
AluI AGCT 5 cut(s) 53, 65, 360, 503, 716
Alw26I GTCTC 9 cut(s) 12, 20, 142, 161, 225, 291, 599, 1089, 1187
AlwI GGATC 5 cut(s) 32, 85, 503, 760, 1121
Aor13HI TCCGGA 1 cut(s) 952
Aor51HI AGCGCT 1 cut(s) 746
AoxI GGCC 2 cut(s) 340, 642
ApeKI GCWGC 2 cut(s) 50, 261
ApoI RAATTY 3 cut(s) 172, 225, 585
AspLEI GCGC 1 cut(s) 747
AspS9I GGNCC 3 cut(s) 346, 643, 851
AsuHPI GGTGA 3 cut(s) 121, 236, 968
AvaII GGWCC 2 cut(s) 346, 851
BanII GRGCYC 1 cut(s) 1046
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 62, 273
BccI CCATC 5 cut(s) 520, 655, 778, 952, 990
BciT130I CCWGG 4 cut(s) 95, 344, 350, 1068
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 9 cut(s) 12, 20, 142, 161, 225, 291, 599, 1089, 1187
BfaI CTAG 3 cut(s) 762, 836, 1028
BfmI CTRYAG 3 cut(s) 48, 178, 326
BfoI RGCGCY 1 cut(s) 748
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 3 cut(s) 51, 184, 262
BlsI GCNGC 3 cut(s) 52, 185, 263
Bme1390I CCNGG 4 cut(s) 95, 344, 350, 1068
Bme18I GGWCC 2 cut(s) 346, 851
BmgT120I GGNCC 3 cut(s) 346, 643, 851
BmiI GGNNCC 1 cut(s) 645
BmrFI CCNGG 4 cut(s) 95, 344, 350, 1068
BmsI GCATC 5 cut(s) 219, 608, 867, 934, 1157
BpmI CTGGAG 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 883
Bsa29I ATCGAT 1 cut(s) 942
BsaBI GATNNNNATC 2 cut(s) 735, 1125
BsaJI CCNNGG 3 cut(s) 349, 444, 459
BsaWI WCCGGW 1 cut(s) 952
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 349, 773
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 2 cut(s) 25, 1203
Bse3DI GCAATG 1 cut(s) 41
Bse8I GATNNNNATC 2 cut(s) 735, 1125
BseAI TCCGGA 1 cut(s) 952
BseBI CCWGG 4 cut(s) 95, 344, 350, 1068
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 3 cut(s) 349, 444, 459
BseGI GGATG 6 cut(s) 210, 512, 599, 647, 982, 1172
BseJI GATNNNNATC 2 cut(s) 735, 1125
BseLI CCNNNNNNNGG 3 cut(s) 147, 349, 773
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 25, 1203
BseRI GAGGAG 1 cut(s) 547
BseXI GCAGC 2 cut(s) 62, 273
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 1007
BshFI GGCC 2 cut(s) 342, 644
BshVI ATCGAT 1 cut(s) 942
BsiSI CCGG 2 cut(s) 107, 953
BslI CCNNNNNNNGG 3 cut(s) 147, 349, 773
BsmAI GTCTC 9 cut(s) 12, 20, 142, 161, 225, 291, 599, 1089, 1187
BsmBI CGTCTC 1 cut(s) 291
BsnI GGCC 2 cut(s) 342, 644
Bsp1286I GDGCHC 1 cut(s) 1046
Bsp13I TCCGGA 1 cut(s) 952
Bsp1407I TGTACA 1 cut(s) 1059
Bsp143I GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
BspACI CCGC 3 cut(s) 124, 184, 190
BspANI GGCC 2 cut(s) 342, 644
BspCNI CTCAG 1 cut(s) 46
BspDI ATCGAT 1 cut(s) 942
BspEI TCCGGA 1 cut(s) 952
BspFNI CGCG 1 cut(s) 1007
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 645
BspMAI CTGCAG 2 cut(s) 52, 330
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 5 cut(s) 32, 85, 503, 760, 1121
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 1 cut(s) 1059
BsrI ACTGG 2 cut(s) 25, 1203
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 3 cut(s) 349, 444, 459
BssMI GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
BssT1I CCWWGG 2 cut(s) 444, 459
Bst2UI CCWGG 4 cut(s) 95, 344, 350, 1068
Bst6I CTCTTC 1 cut(s) 1032
BstAUI TGTACA 1 cut(s) 1059
BstC8I GCNNGC 5 cut(s) 48, 104, 208, 674, 779
BstDEI CTNAG 1 cut(s) 54
BstENI CCTNNNNNAGG 1 cut(s) 771
BstF5I GGATG 6 cut(s) 210, 512, 599, 647, 982, 1172
BstFNI CGCG 1 cut(s) 1007
BstH2I RGCGCY 1 cut(s) 748
BstHHI GCGC 1 cut(s) 747
BstKTI GATC 8 cut(s) 40, 93, 511, 577, 632, 755, 939, 1129
BstMAI GTCTC 9 cut(s) 12, 20, 142, 161, 225, 291, 599, 1089, 1187
BstMBI GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
BstMWI GCNNNNNNNGC 2 cut(s) 56, 189
BstNI CCWGG 4 cut(s) 95, 344, 350, 1068
BstNSI RCATGY 2 cut(s) 210, 1163
BstSCI CCNGG 4 cut(s) 93, 342, 348, 1066
BstSFI CTRYAG 3 cut(s) 48, 178, 326
BstUI CGCG 1 cut(s) 1007
BstV1I GCAGC 2 cut(s) 62, 273
Bsu15I ATCGAT 1 cut(s) 942
BsuRI GGCC 2 cut(s) 342, 644
BsuTUI ATCGAT 1 cut(s) 942
BtsCI GGATG 6 cut(s) 210, 512, 599, 647, 982, 1172
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 3 cut(s) 18, 152, 1210
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 5 cut(s) 48, 104, 208, 674, 779
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 747
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 346, 643, 851
ClaI ATCGAT 1 cut(s) 942
Csp6I GTAC 4 cut(s) 98, 789, 916, 1060
CviAII CATG 4 cut(s) 207, 452, 1160, 1175
CviQI GTAC 4 cut(s) 98, 789, 916, 1060
DdeI CTNAG 1 cut(s) 54
DpnI GATC 8 cut(s) 39, 92, 510, 576, 631, 754, 938, 1128
DpnII GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
Eco130I CCWWGG 2 cut(s) 444, 459
Eco24I GRGCYC 1 cut(s) 1046
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 346, 851
Eco47III AGCGCT 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 1056
EcoNI CCTNNNNNAGG 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 225
EcoRII CCWGG 4 cut(s) 93, 342, 348, 1066
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 444, 459
EcoT22I ATGCAT 2 cut(s) 212, 949
EcoT38I GRGCYC 1 cut(s) 1046
ErhI CCWWGG 2 cut(s) 444, 459
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 4 cut(s) 210, 455, 1163, 1178
FatI CATG 4 cut(s) 206, 451, 1159, 1174
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 3 cut(s) 51, 184, 262
FokI GGATG 6 cut(s) 197, 499, 586, 634, 969, 1179
FriOI GRGCYC 1 cut(s) 1046
Fsp4HI GCNGC 3 cut(s) 51, 184, 262
FspBI CTAG 3 cut(s) 762, 836, 1028
GlaI GCGC 1 cut(s) 746
GluI GCNGC 3 cut(s) 51, 184, 262
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 148
HaeII RGCGCY 1 cut(s) 748
HaeIII GGCC 2 cut(s) 342, 644
HapII CCGG 2 cut(s) 107, 953
HhaI GCGC 1 cut(s) 747
Hin1II CATG 4 cut(s) 210, 455, 1163, 1178
Hin6I GCGC 1 cut(s) 745
HinP1I GCGC 1 cut(s) 745
HinfI GANTC 8 cut(s) 305, 424, 455, 607, 815, 907, 992, 1195
HpaII CCGG 2 cut(s) 107, 953
HphI GGTGA 3 cut(s) 121, 236, 968
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 8 cut(s) 271, 565, 591, 604, 652, 752, 936, 1131
Hpy188III TCNNGA 7 cut(s) 222, 290, 421, 452, 627, 740, 953
Hpy8I GTNNAC 1 cut(s) 434
HpyAV CCTTC 3 cut(s) 414, 789, 983
HpyCH4V TGCA 9 cut(s) 50, 206, 210, 261, 328, 399, 689, 832, 947
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 189
HpyF3I CTNAG 1 cut(s) 54
Hsp92II CATG 4 cut(s) 210, 455, 1163, 1178
HspAI GCGC 1 cut(s) 745
Kpn2I TCCGGA 1 cut(s) 952
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
LmnI GCTCC 2 cut(s) 560, 713
Lsp1109I GCAGC 2 cut(s) 62, 273
LweI GCATC 5 cut(s) 219, 608, 867, 934, 1157
MaeI CTAG 3 cut(s) 762, 836, 1028
MaeIII GTNAC 4 cut(s) 82, 114, 242, 321
MalI GATC 8 cut(s) 39, 92, 510, 576, 631, 754, 938, 1128
MboI GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
MboII GAAGA 2 cut(s) 419, 1049
MfeI CAATTG 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 1046
MluCI AATT 9 cut(s) 172, 225, 256, 416, 469, 550, 585, 866, 881
MluI ACGCGT 1 cut(s) 1005
MlyI GAGTC 2 cut(s) 601, 1189
MmeI TCCRAC 1 cut(s) 1144
MnlI CCTC 9 cut(s) 49, 89, 211, 224, 245, 568, 612, 777, 1033
Mph1103I ATGCAT 2 cut(s) 212, 949
MroI TCCGGA 1 cut(s) 952
MseI TTAA 2 cut(s) 318, 1210
MslI CAYNNNNRTG 1 cut(s) 987
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 107, 953
MspR9I CCNGG 4 cut(s) 95, 344, 350, 1068
MunI CAATTG 1 cut(s) 256
MvaI CCWGG 4 cut(s) 95, 344, 350, 1068
MvnI CGCG 1 cut(s) 1007
MwoI GCNNNNNNNGC 2 cut(s) 56, 189
NdeII GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
NlaIII CATG 4 cut(s) 210, 455, 1163, 1178
NlaIV GGNNCC 1 cut(s) 645
NmuCI GTSAC 3 cut(s) 82, 114, 242
NsiI ATGCAT 2 cut(s) 212, 949
NspI RCATGY 2 cut(s) 210, 1163
PaeI GCATGC 1 cut(s) 210
PagI TCATGA 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 302
PciI ACATGT 1 cut(s) 1159
PfeI GAWTC 6 cut(s) 305, 424, 455, 815, 907, 992
PflFI GACNNNGTC 1 cut(s) 80
PflMI CCANNNNNTGG 1 cut(s) 147
PkrI GCNGC 3 cut(s) 52, 185, 263
PleI GAGTC 2 cut(s) 601, 1189
PpsI GAGTC 2 cut(s) 601, 1189
PscI ACATGT 1 cut(s) 1159
PsiI TTATAA 1 cut(s) 1086
Psp6I CCWGG 4 cut(s) 93, 342, 348, 1066
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 4 cut(s) 93, 342, 348, 1066
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 3 cut(s) 346, 643, 851
PstI CTGCAG 2 cut(s) 52, 330
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 4 cut(s) 99, 790, 917, 1061
RsaNI GTAC 4 cut(s) 98, 789, 916, 1060
RseI CAYNNNNRTG 1 cut(s) 987
SaqAI TTAA 2 cut(s) 318, 1210
SatI GCNGC 3 cut(s) 51, 184, 262
Sau3AI GATC 8 cut(s) 37, 90, 508, 574, 629, 752, 936, 1126
Sau96I GGNCC 3 cut(s) 346, 643, 851
SchI GAGTC 2 cut(s) 601, 1189
ScrFI CCNGG 4 cut(s) 95, 344, 350, 1068
SduI GDGCHC 1 cut(s) 1046
SfaNI GCATC 5 cut(s) 219, 608, 867, 934, 1157
SfcI CTRYAG 3 cut(s) 48, 178, 326
SinI GGWCC 2 cut(s) 346, 851
SmiMI CAYNNNNRTG 1 cut(s) 987
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
SphI GCATGC 1 cut(s) 210
Sse9I AATT 9 cut(s) 172, 225, 256, 416, 469, 550, 585, 866, 881
SsiI CCGC 3 cut(s) 124, 184, 190
SspI AATATT 1 cut(s) 1090
SspMI CTAG 3 cut(s) 762, 836, 1028
StyD4I CCNGG 4 cut(s) 93, 342, 348, 1066
StyI CCWWGG 2 cut(s) 444, 459
TaqI TCGA 5 cut(s) 223, 422, 610, 924, 942
TasI AATT 9 cut(s) 172, 225, 256, 416, 469, 550, 585, 866, 881
TatI WGTACW 1 cut(s) 1059
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 6 cut(s) 305, 424, 455, 815, 907, 992
Tru1I TTAA 2 cut(s) 318, 1210
Tru9I TTAA 2 cut(s) 318, 1210
TscAI CASTG 3 cut(s) 25, 152, 1210
TseFI GTSAC 3 cut(s) 82, 114, 242
TseI GCWGC 2 cut(s) 50, 261
Tsp45I GTSAC 3 cut(s) 82, 114, 242
TspDTI ATGAA 2 cut(s) 286, 1163
TspRI CASTG 3 cut(s) 25, 152, 1210
Tth111I GACNNNGTC 1 cut(s) 80
Van91I CCANNNNNTGG 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 346, 851
XagI CCTNNNNNAGG 1 cut(s) 771
XapI RAATTY 3 cut(s) 172, 225, 585
XceI RCATGY 2 cut(s) 210, 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XspI CTAG 3 cut(s) 762, 836, 1028
Zsp2I ATGCAT 2 cut(s) 212, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.