FvH4_4g06310

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002699
Physical Location & Seq
Reverse (-)
5620414 .. 5621547
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06310.t1

Sequence Viewer

Length: 1134 bp
ATGTTCCACCAAGACCTCCTCACAGACATCCTTCTACGCCTCCCCATCAAGTCTCTCCTTCGCTTCAAATGCGTCTCCAAGCAGTGGCTTTCTCTCATCTCCAGCCCCCAGTTCCACCGCCGCCGCCGCCGCCTTCTCGCCTCCTCCGGCATCCTTTTGTGTCAAACAACCGATCTCATCTACCACACATCTCTCGCCGGAACCAACTCCGAACCACCCTTTACATCCCTGAGCTTCATCGGTGATCCGGCAGGTGTCAACATCCTCCAGTCCAGCAATGGCCTGCTCTTGTGCTGCAGCAGCTTTCACACTGCAAGAATGGGTTTAGGCCGCAGCTATTACATCTGCAATCCTTCCACGCAGCAGTTCTCCATTCTTCCTAAAGCAGACTTCGAAAACGTATCCAAAACCATTTCTGGAGCGTACTTGGTTTTCGATTCGTCCAAGTCGCCTCACTACCAAGTCGTCTCTGTTCGAAGATGTTCTTCCCCATCATCATCATCATCATCTTCTTCCAATAGCTACCACATCGTTACTTACTCGTCGGAGACTCGGGCTTGGAGGCTTTCCGGGTCTCCTTTCACAGCCCCAGTCATGGTGTTTGACGATGGTGTGCTTTGGAATGGTTCAATCCACTGGCTTAGTCCTACTGGGGCTTCGTTGTTTTTCCACATTGATCAAGAACGCCTCGGAACAATGCCTGCTCCTCCATCAGCTGAGAAATGGAGCAACCGAAGGTTGAGGTACTTCGCCGAGTCCGGTGGCCATTTACACTTGATTGAAGTCTACGGCAGCGCCACTCGATTCCATGTCTTTGAGATGGAGAGGGATTACTCTAGCTGGCTTCCCAAGTACCAAGTTGACATTTCAGCGATTGTTGATGCATTTCCTGTGATGGTTCGGAGCTTCCCTGACAGCAATGATCCTGTTTTTTATGCATTTAGTCTGCTACTCGTGAAAGAAGATGAAGAGGACTCATTGCTGGTGTTGCATGTTCCTGGTGAGTTTCTGTTGTATAATCTTAGGAACGGGACCTTCAAGAAGCTTGGTCCAATATCCTCAGAAGAAAATATTGCATTACAGACTGGATGTTTCCATGCTTATCAGTTTACAGAGACTTTGGCTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

378

Amino Acids

42.24

Weight (kDa)

7.21

Isoelectric Point (pI)

67.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 40 1.7e-08 F-box domain
FBA_3 PF08268 84 - 245 1.5e-09 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 84 - 282 9.1e-08 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 87 - 303 1.4e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 1 cut(s) 786
AciI CCGC 6 cut(s) 118, 121, 124, 127, 130, 331
AclWI GGATC 2 cut(s) 239, 917
AcoI YGGCCR 1 cut(s) 763
AfaI GTAC 3 cut(s) 425, 746, 854
AfiI CCNNNNNNNGG 2 cut(s) 84, 595
AgsI TTSAA 4 cut(s) 67, 630, 782, 1039
AjnI CCWGG 1 cut(s) 997
AluBI AGCT 8 cut(s) 234, 303, 336, 522, 716, 840, 906, 1045
AluI AGCT 8 cut(s) 234, 303, 336, 522, 716, 840, 906, 1045
Alw26I GTCTC 6 cut(s) 57, 79, 472, 542, 579, 1109
AlwI GGATC 2 cut(s) 239, 917
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 3 cut(s) 280, 328, 763
ApeKI GCWGC 6 cut(s) 294, 297, 300, 333, 361, 792
Asp700I GAANNNNTTC 1 cut(s) 481
AspLEI GCGC 1 cut(s) 797
AspS9I GGNCC 2 cut(s) 1032, 1049
AsuC2I CCSGG 1 cut(s) 571
AsuHPI GGTGA 2 cut(s) 254, 1013
AsuII TTCGAA 2 cut(s) 393, 475
AvaI CYCGRG 1 cut(s) 552
AvaII GGWCC 2 cut(s) 1032, 1049
BalI TGGCCA 1 cut(s) 765
BauI CACGAG 1 cut(s) 953
BbvI GCAGC 6 cut(s) 281, 309, 312, 345, 373, 804
BccI CCATC 6 cut(s) 53, 499, 602, 718, 814, 889
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 1 cut(s) 999
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 6 cut(s) 57, 79, 472, 542, 579, 1109
BfaI CTAG 1 cut(s) 837
BfmI CTRYAG 1 cut(s) 295
BfoI RGCGCY 1 cut(s) 798
BfuAI ACCTGC 1 cut(s) 242
BfuI GTATCC 1 cut(s) 412
Bme1390I CCNGG 2 cut(s) 571, 999
Bme18I GGWCC 2 cut(s) 1032, 1049
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 2 cut(s) 1032, 1049
BmiI GGNNCC 2 cut(s) 202, 1033
BmrFI CCNGG 2 cut(s) 571, 999
BmrI ACTGGG 3 cut(s) 103, 584, 660
BmsI GCATC 2 cut(s) 159, 871
BmuI ACTGGG 3 cut(s) 103, 584, 660
BpmI CTGGAG 3 cut(s) 85, 251, 438
Bpu10I CCTNAGC 1 cut(s) 230
Bpu14I TTCGAA 2 cut(s) 393, 475
BpuMI CCSGG 1 cut(s) 571
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 1 cut(s) 688
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 595
Bse1I ACTGG 6 cut(s) 109, 268, 590, 641, 655, 1090
Bse3DI GCAATG 3 cut(s) 283, 925, 977
BseBI CCWGG 1 cut(s) 999
BseDI CCNNGG 1 cut(s) 688
BseGI GGATG 5 cut(s) 27, 150, 224, 261, 1094
BseLI CCNNNNNNNGG 2 cut(s) 84, 595
BseMI GCAATG 3 cut(s) 283, 925, 977
BseMII CTCAG 3 cut(s) 221, 708, 1074
BseNI ACTGG 6 cut(s) 109, 268, 590, 641, 655, 1090
BseRI GAGGAG 3 cut(s) 8, 133, 696
BseXI GCAGC 6 cut(s) 281, 309, 312, 345, 373, 804
BshFI GGCC 3 cut(s) 282, 330, 765
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 5 cut(s) 147, 198, 248, 570, 759
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 2 cut(s) 84, 595
BsmAI GTCTC 6 cut(s) 57, 79, 472, 542, 579, 1109
BsmBI CGTCTC 2 cut(s) 79, 472
BsmFI GGGAC 1 cut(s) 1045
BsnI GGCC 3 cut(s) 282, 330, 765
Bso31I GGTCTC 1 cut(s) 579
BsoBI CYCGRG 1 cut(s) 552
Bsp119I TTCGAA 2 cut(s) 393, 475
Bsp143I GATC 4 cut(s) 172, 244, 676, 922
BspACI CCGC 6 cut(s) 118, 121, 124, 127, 130, 331
BspANI GGCC 3 cut(s) 282, 330, 765
BspCNI CTCAG 3 cut(s) 222, 709, 1073
BspLI GGNNCC 2 cut(s) 202, 1033
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 1 cut(s) 242
BspPI GGATC 2 cut(s) 239, 917
BspT104I TTCGAA 2 cut(s) 393, 475
BspTNI GGTCTC 1 cut(s) 579
BsrDI GCAATG 3 cut(s) 283, 925, 977
BsrI ACTGG 6 cut(s) 109, 268, 590, 641, 655, 1090
BssECI CCNNGG 1 cut(s) 688
BssMI GATC 4 cut(s) 172, 244, 676, 922
BssSI CACGAG 1 cut(s) 953
Bst2BI CACGAG 1 cut(s) 953
Bst2UI CCWGG 1 cut(s) 999
Bst6I CTCTTC 1 cut(s) 963
BstBI TTCGAA 2 cut(s) 393, 475
BstC8I GCNNGC 3 cut(s) 284, 702, 842
BstDEI CTNAG 5 cut(s) 230, 641, 717, 1022, 1060
BstF5I GGATG 5 cut(s) 27, 150, 224, 261, 1094
BstH2I RGCGCY 1 cut(s) 798
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 4 cut(s) 175, 247, 679, 925
BstMAI GTCTC 6 cut(s) 57, 79, 472, 542, 579, 1109
BstMBI GATC 4 cut(s) 172, 244, 676, 922
BstMWI GCNNNNNNNGC 5 cut(s) 69, 126, 129, 300, 988
BstNI CCWGG 1 cut(s) 999
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 2 cut(s) 569, 997
BstSFI CTRYAG 1 cut(s) 295
BstV1I GCAGC 6 cut(s) 281, 309, 312, 345, 373, 804
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 3 cut(s) 282, 330, 765
BtsCI GGATG 5 cut(s) 27, 150, 224, 261, 1094
BtsI GCAGTG 2 cut(s) 89, 309
BtsIMutI CAGTG 3 cut(s) 89, 309, 634
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 3 cut(s) 284, 702, 842
CfoI GCGC 1 cut(s) 797
Cfr13I GGNCC 2 cut(s) 1032, 1049
CseI GACGC 1 cut(s) 61
Csp6I GTAC 3 cut(s) 424, 745, 853
CviAII CATG 4 cut(s) 595, 809, 992, 1097
CviQI GTAC 3 cut(s) 424, 745, 853
DdeI CTNAG 5 cut(s) 230, 641, 717, 1022, 1060
DpnI GATC 4 cut(s) 174, 246, 678, 924
DpnII GATC 4 cut(s) 172, 244, 676, 922
EaeI YGGCCR 1 cut(s) 763
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Eco31I GGTCTC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 1032, 1049
Eco88I CYCGRG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 997
EcoT22I ATGCAT 2 cut(s) 886, 940
Esp3I CGTCTC 2 cut(s) 79, 472
FaeI CATG 4 cut(s) 598, 812, 995, 1100
FaiI YATR 6 cut(s) 596, 810, 936, 993, 1017, 1098
FalI AAGNNNNNCTT 2 cut(s) 469, 501
FaqI GGGAC 1 cut(s) 1045
FatI CATG 4 cut(s) 594, 808, 991, 1096
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 1 cut(s) 786
FokI GGATG 5 cut(s) 14, 137, 211, 248, 1101
FspBI CTAG 1 cut(s) 837
GlaI GCGC 1 cut(s) 796
GsuI CTGGAG 3 cut(s) 85, 251, 438
HaeII RGCGCY 1 cut(s) 798
HaeIII GGCC 3 cut(s) 282, 330, 765
HapII CCGG 5 cut(s) 147, 198, 248, 570, 759
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 797
Hin1II CATG 4 cut(s) 598, 812, 995, 1100
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HincII GTYRAC 2 cut(s) 259, 862
HindII GTYRAC 2 cut(s) 259, 862
HindIII AAGCTT 1 cut(s) 1043
HinfI GANTC 5 cut(s) 437, 550, 755, 804, 974
HpaII CCGG 5 cut(s) 147, 198, 248, 570, 759
HphI GGTGA 2 cut(s) 254, 1013
Hpy166II GTNNAC 4 cut(s) 259, 787, 862, 1110
Hpy188I TCNGA 5 cut(s) 211, 547, 692, 903, 1063
Hpy188III TCNNGA 4 cut(s) 417, 680, 955, 1039
Hpy8I GTNNAC 4 cut(s) 259, 787, 862, 1110
Hpy99I CGWCG 1 cut(s) 547
HpyAV CCTTC 6 cut(s) 41, 68, 143, 363, 729, 1045
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 7 cut(s) 297, 314, 348, 884, 938, 991, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 69, 126, 129, 300, 988
HpyF3I CTNAG 5 cut(s) 230, 641, 717, 1022, 1060
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 4 cut(s) 598, 812, 995, 1100
HspAI GCGC 1 cut(s) 795
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 4 cut(s) 172, 244, 676, 922
LmnI GCTCC 4 cut(s) 419, 709, 726, 903
Lsp1109I GCAGC 6 cut(s) 281, 309, 312, 345, 373, 804
LweI GCATC 2 cut(s) 159, 871
MaeI CTAG 1 cut(s) 837
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 532
MalI GATC 4 cut(s) 174, 246, 678, 924
MboI GATC 4 cut(s) 172, 244, 676, 922
MboII GAAGA 8 cut(s) 368, 477, 489, 501, 504, 974, 980, 1076
MlsI TGGCCA 1 cut(s) 765
MluNI TGGCCA 1 cut(s) 765
MlyI GAGTC 3 cut(s) 544, 764, 968
MmeI TCCRAC 1 cut(s) 525
Mox20I TGGCCA 1 cut(s) 765
Mph1103I ATGCAT 2 cut(s) 886, 940
MroXI GAANNNNTTC 1 cut(s) 481
MscI TGGCCA 1 cut(s) 765
Msp20I TGGCCA 1 cut(s) 765
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 5 cut(s) 147, 198, 248, 570, 759
MspR9I CCNGG 2 cut(s) 571, 999
MvaI CCWGG 1 cut(s) 999
MwoI GCNNNNNNNGC 5 cut(s) 69, 126, 129, 300, 988
NciI CCSGG 1 cut(s) 571
NdeII GATC 4 cut(s) 172, 244, 676, 922
NlaIII CATG 4 cut(s) 598, 812, 995, 1100
NlaIV GGNNCC 2 cut(s) 202, 1033
NmeAIII GCCGAG 1 cut(s) 778
NsiI ATGCAT 2 cut(s) 886, 940
NspI RCATGY 1 cut(s) 995
NspV TTCGAA 2 cut(s) 393, 475
PaqCI CACCTGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 481
PfeI GAWTC 2 cut(s) 437, 804
PflMI CCANNNNNTGG 1 cut(s) 84
PleI GAGTC 3 cut(s) 544, 763, 968
PpsI GAGTC 3 cut(s) 544, 763, 968
PpuMI RGGWCCY 1 cut(s) 1032
Psp5II RGGWCCY 1 cut(s) 1032
Psp6I CCWGG 1 cut(s) 997
PspGI CCWGG 1 cut(s) 997
PspN4I GGNNCC 2 cut(s) 202, 1033
PspPI GGNCC 2 cut(s) 1032, 1049
PspPPI RGGWCCY 1 cut(s) 1032
PstI CTGCAG 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 3 cut(s) 425, 746, 854
RsaNI GTAC 3 cut(s) 424, 745, 853
Sau3AI GATC 4 cut(s) 172, 244, 676, 922
Sau96I GGNCC 2 cut(s) 1032, 1049
SchI GAGTC 3 cut(s) 544, 764, 968
ScrFI CCNGG 2 cut(s) 571, 999
SfaNI GCATC 2 cut(s) 159, 871
SfcI CTRYAG 1 cut(s) 295
SfuI TTCGAA 2 cut(s) 393, 475
SinI GGWCC 2 cut(s) 1032, 1049
SsiI CCGC 6 cut(s) 118, 121, 124, 127, 130, 331
SspI AATATT 1 cut(s) 1072
SspMI CTAG 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 569, 997
TaiI ACGT 1 cut(s) 402
TaqI TCGA 4 cut(s) 393, 435, 475, 802
TauI GCSGC 5 cut(s) 123, 126, 129, 132, 333
TfiI GAWTC 2 cut(s) 437, 804
TscAI CASTG 3 cut(s) 89, 316, 641
TseI GCWGC 6 cut(s) 294, 297, 300, 333, 361, 792
TspDTI ATGAA 2 cut(s) 226, 981
TspRI CASTG 3 cut(s) 89, 316, 641
Van91I CCANNNNNTGG 1 cut(s) 84
VpaK11BI GGWCC 2 cut(s) 1032, 1049
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 481
XspI CTAG 1 cut(s) 837
Zsp2I ATGCAT 2 cut(s) 886, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.