Rh4DG075400

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002699
Physical Location & Seq
Reverse (-)
11160776 .. 11162928
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG075400.1

Sequence Viewer

Length: 1002 bp
ATGCTCCCCCAAGATCTCATAACCGAGATCCTCCTACGCCTCCCCGTCAAGTCCCTCCTCCGCTTCAAATGCGTCTCCAAGCAGTGGCTCTCTCTCATCTCCAGCCCCCAGTTCCGCCACCACCGCCGCGGCCTCACCTCCTCCGGCCTCATCTTGTGTCGAACAACCGACCTCATCCACCACATATCACTACTTGGAAGCAACAACTCCGACCAGCCCTTCACTGCCCTCAGCTTCGTTCATGACTCGGCAGGTATCAAAATCCTTCAGTCCAGCCATGGCTTGCTCTTGTGCCGCAGCCTTTACAAACTGGGTAAAACCTGCAGCTATTACATCTGCAATCCTTCAACAAAGAAATTCTCAGTTCTTCCTCATTATAGTGCTGAACCGGATTATGTATCCGGGTCGGAATCCATAACTATTTCTGGTGTTTATTTAGTTTTCAATCCCACTAAGTCACCTCACTACCAAGTTGCCTGCATTCGAATGTGTTCTTCCTCATTCAATAGCTACCAAGTTGAGATATACTCATCTGCCACTCGGGCTTGGAGGCTTTCCGGGTCTCCTTTCCCTGCCCCTTTCGACATGGTGTTTGACAATGGGGTGCTTTGGAATGCTGCAATACACTGGATCAGTCCAACTGGGGCGTCGTTATGCTTTGATATTGATCAAGAACGCCTTGGAGCAATGCCTGGTCTTCCGTCAAATGAGAAATGGAGCAGGAGAAGGTTGAGGTATTTCGGCGAGTCTGGTGGGCATTTGCACCTAACTGAAATCTATGGCTGCACCTCTCAATTCCTAGTCTTTGAGATGGAGAGGGATTACTCTAGCTGGGTTCCCAAGTACAATGTCGATCTTGCTCCAGTTGTAGATGCATTTCCGGAGATGGTTCGGAACTGCATTTTCCGTAATGATTCTGTTTCTTATGCATTTAGTCTACTCTGTTTGCAAGAGGATGAAGATGACTCATTGCTGTTGCTGCACATTCCTGATACTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

333

Amino Acids

37.63

Weight (kDa)

6.85

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 40 1.4e-10 F-box domain
F-box-like PF12937 2 - 40 6.5e-06 F-box-like
FBA_3 PF08268 76 - 230 1.5e-09 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 82 - 279 2.4e-07 KIB1-4 beta-propeller
FBA_1 PF07734 86 - 248 2.2e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 242, 329
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 1 cut(s) 937
AccII CGCG 1 cut(s) 129
AccIII TCCGGA 1 cut(s) 880
AciI CCGC 6 cut(s) 61, 115, 124, 127, 129, 295
AclWI GGATC 2 cut(s) 22, 638
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 647
AfaI GTAC 1 cut(s) 845
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 4 cut(s) 67, 348, 445, 505
AjnI CCWGG 1 cut(s) 691
AluBI AGCT 4 cut(s) 234, 327, 510, 831
AluI AGCT 4 cut(s) 234, 327, 510, 831
Alw26I GTCTC 2 cut(s) 79, 567
AlwI GGATC 2 cut(s) 22, 638
Ama87I CYCGRG 1 cut(s) 540
Aor13HI TCCGGA 1 cut(s) 880
AoxI GGCC 2 cut(s) 130, 145
ApeKI GCWGC 5 cut(s) 297, 324, 617, 783, 979
ApoI RAATTY 1 cut(s) 356
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
Asp700I GAANNNNTTC 1 cut(s) 490
AsuC2I CCSGG 2 cut(s) 403, 559
AsuHPI GGTGA 2 cut(s) 127, 450
AsuII TTCGAA 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 689
BbvCI CCTCAGC 1 cut(s) 230
BbvI GCAGC 5 cut(s) 309, 336, 604, 770, 966
BccI CCATC 2 cut(s) 805, 880
BciT130I CCWGG 1 cut(s) 693
BciVI GTATCC 1 cut(s) 409
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 2 cut(s) 403, 559
BcoDI GTCTC 2 cut(s) 79, 567
BfaI CTAG 2 cut(s) 800, 828
BfmI CTRYAG 1 cut(s) 322
BfuAI ACCTGC 2 cut(s) 242, 329
BfuI GTATCC 1 cut(s) 409
BglI GCCNNNNNGGC 1 cut(s) 542
BglII AGATCT 1 cut(s) 13
BisI GCNGC 8 cut(s) 127, 130, 295, 298, 325, 618, 784, 980
BlsI GCNGC 8 cut(s) 128, 131, 296, 299, 326, 619, 785, 981
Bme1390I CCNGG 3 cut(s) 403, 559, 693
BmeT110I CYCGRG 1 cut(s) 540
BmiI GGNNCC 1 cut(s) 837
BmrFI CCNGG 3 cut(s) 403, 559, 693
BmrI ACTGGG 3 cut(s) 103, 320, 651
BmsI GCATC 1 cut(s) 862
BmuI ACTGGG 3 cut(s) 103, 320, 651
BpiI GAAGAC 1 cut(s) 689
BplI GAGNNNNNCTC 2 cut(s) 512, 544
BpmI CTGGAG 2 cut(s) 85, 846
Bpu10I CCTNAGC 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 484
BpuMI CCSGG 2 cut(s) 403, 559
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 1 cut(s) 647
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 3 cut(s) 127, 277, 679
BsaWI WCCGGW 2 cut(s) 388, 880
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse1I ACTGG 5 cut(s) 109, 315, 632, 646, 863
Bse3DI GCAATG 2 cut(s) 693, 968
Bse8I GATNNNNATC 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 880
BseBI CCWGG 1 cut(s) 693
BseDI CCNNGG 3 cut(s) 127, 277, 679
BseGI GGATG 2 cut(s) 174, 961
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMI GCAATG 2 cut(s) 693, 968
BseMII CTCAG 2 cut(s) 244, 375
BseNI ACTGG 5 cut(s) 109, 315, 632, 646, 863
BseRI GAGGAG 2 cut(s) 47, 130
BseXI GCAGC 5 cut(s) 309, 336, 604, 770, 966
BseYI CCCAGC 1 cut(s) 831
BsgI GTGCAG 2 cut(s) 769, 965
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 132, 147
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 5 cut(s) 144, 389, 402, 558, 881
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 2 cut(s) 79, 567
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 37
BsmI GAATGC 2 cut(s) 480, 619
BsnI GGCC 2 cut(s) 132, 147
Bso31I GGTCTC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 540
Bsp119I TTCGAA 1 cut(s) 484
Bsp13I TCCGGA 1 cut(s) 880
Bsp143I GATC 5 cut(s) 13, 27, 630, 667, 853
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 6 cut(s) 61, 115, 124, 127, 129, 295
BspANI GGCC 2 cut(s) 132, 147
BspCNI CTCAG 2 cut(s) 243, 374
BspEI TCCGGA 1 cut(s) 880
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 837
BspMAI CTGCAG 1 cut(s) 326
BspMI ACCTGC 2 cut(s) 242, 329
BspPI GGATC 2 cut(s) 22, 638
BspT104I TTCGAA 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 2 cut(s) 693, 968
BsrI ACTGG 5 cut(s) 109, 315, 632, 646, 863
BssECI CCNNGG 3 cut(s) 127, 277, 679
BssMI GATC 5 cut(s) 13, 27, 630, 667, 853
BssNI GRCGYC 1 cut(s) 647
BssT1I CCWWGG 2 cut(s) 277, 679
Bst2UI CCWGG 1 cut(s) 693
BstACI GRCGYC 1 cut(s) 647
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 284, 478
BstDEI CTNAG 3 cut(s) 230, 361, 453
BstDSI CCRYGG 2 cut(s) 127, 277
BstF5I GGATG 2 cut(s) 174, 961
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 5 cut(s) 16, 30, 633, 670, 856
BstMAI GTCTC 2 cut(s) 79, 567
BstMBI GATC 5 cut(s) 13, 27, 630, 667, 853
BstMWI GCNNNNNNNGC 4 cut(s) 69, 123, 542, 979
BstNI CCWGG 1 cut(s) 693
BstSCI CCNGG 3 cut(s) 401, 557, 691
BstSFI CTRYAG 1 cut(s) 322
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 5 cut(s) 309, 336, 604, 770, 966
BstV2I GAAGAC 1 cut(s) 689
BstX2I RGATCY 2 cut(s) 13, 27
BstYI RGATCY 2 cut(s) 13, 27
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 2 cut(s) 132, 147
BtgI CCRYGG 2 cut(s) 127, 277
BtsCI GGATG 2 cut(s) 174, 961
BtsI GCAGTG 2 cut(s) 89, 222
BtsIMutI CAGTG 3 cut(s) 89, 222, 625
BveI ACCTGC 2 cut(s) 242, 329
Cac8I GCNNGC 2 cut(s) 284, 478
CciI TCATGA 1 cut(s) 241
Cfr42I CCGCGG 1 cut(s) 130
CseI GACGC 2 cut(s) 61, 636
Csp6I GTAC 1 cut(s) 844
CviAII CATG 3 cut(s) 242, 278, 586
CviQI GTAC 1 cut(s) 844
DdeI CTNAG 3 cut(s) 230, 361, 453
DpnI GATC 5 cut(s) 15, 29, 632, 669, 855
DpnII GATC 5 cut(s) 13, 27, 630, 667, 853
EciI GGCGGA 1 cut(s) 104
Eco130I CCWWGG 2 cut(s) 277, 679
Eco31I GGTCTC 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 691
EcoT14I CCWWGG 2 cut(s) 277, 679
EcoT22I ATGCAT 2 cut(s) 877, 931
ErhI CCWWGG 2 cut(s) 277, 679
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 3 cut(s) 245, 281, 589
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FaqI GGGAC 1 cut(s) 37
FatI CATG 3 cut(s) 241, 277, 585
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 937
Fnu4HI GCNGC 8 cut(s) 127, 130, 295, 298, 325, 618, 784, 980
FokI GGATG 2 cut(s) 161, 968
Fsp4HI GCNGC 8 cut(s) 127, 130, 295, 298, 325, 618, 784, 980
FspBI CTAG 2 cut(s) 800, 828
GluI GCNGC 8 cut(s) 127, 130, 295, 298, 325, 618, 784, 980
GsaI CCCAGC 1 cut(s) 835
GsuI CTGGAG 2 cut(s) 85, 846
HaeIII GGCC 2 cut(s) 132, 147
HapII CCGG 5 cut(s) 144, 389, 402, 558, 881
HgaI GACGC 2 cut(s) 61, 636
Hin1I GRCGYC 1 cut(s) 647
Hin1II CATG 3 cut(s) 245, 281, 589
HinfI GANTC 5 cut(s) 245, 410, 746, 914, 965
HpaII CCGG 5 cut(s) 144, 389, 402, 558, 881
HphI GGTGA 2 cut(s) 127, 450
Hpy166II GTNNAC 1 cut(s) 938
Hpy188I TCNGA 3 cut(s) 211, 409, 894
Hpy188III TCNNGA 4 cut(s) 242, 671, 881, 989
Hpy8I GTNNAC 1 cut(s) 938
Hpy99I CGWCG 1 cut(s) 652
HpyAV CCTTC 4 cut(s) 229, 275, 354, 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 123, 542, 979
HpyF3I CTNAG 3 cut(s) 230, 361, 453
Hsp92I GRCGYC 1 cut(s) 647
Hsp92II CATG 3 cut(s) 245, 281, 589
Kpn2I TCCGGA 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 667
KspI CCGCGG 1 cut(s) 130
Kzo9I GATC 5 cut(s) 13, 27, 630, 667, 853
LmnI GCTCC 4 cut(s) 9, 683, 717, 865
Lsp1109I GCAGC 5 cut(s) 309, 336, 604, 770, 966
LweI GCATC 1 cut(s) 862
MaeI CTAG 2 cut(s) 800, 828
MaeIII GTNAC 1 cut(s) 456
MalI GATC 5 cut(s) 15, 29, 632, 669, 855
MboI GATC 5 cut(s) 13, 27, 630, 667, 853
MboII GAAGA 4 cut(s) 359, 486, 689, 971
MflI RGATCY 2 cut(s) 13, 27
MluCI AATT 2 cut(s) 356, 794
MlyI GAGTC 3 cut(s) 239, 755, 959
MmeI TCCRAC 3 cut(s) 234, 387, 662
Mph1103I ATGCAT 2 cut(s) 877, 931
MroI TCCGGA 1 cut(s) 880
MroXI GAANNNNTTC 1 cut(s) 490
MslI CAYNNNNRTG 2 cut(s) 378, 485
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 5 cut(s) 144, 389, 402, 558, 881
MspR9I CCNGG 3 cut(s) 403, 559, 693
Mva1269I GAATGC 2 cut(s) 480, 619
MvaI CCWGG 1 cut(s) 693
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 69, 123, 542, 979
NciI CCSGG 2 cut(s) 403, 559
NcoI CCATGG 1 cut(s) 277
NdeII GATC 5 cut(s) 13, 27, 630, 667, 853
NlaIII CATG 3 cut(s) 245, 281, 589
NlaIV GGNNCC 1 cut(s) 837
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 456
NsiI ATGCAT 2 cut(s) 877, 931
NspV TTCGAA 1 cut(s) 484
PagI TCATGA 1 cut(s) 241
PctI GAATGC 2 cut(s) 480, 619
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 410, 914
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 8 cut(s) 128, 131, 296, 299, 326, 619, 785, 981
PleI GAGTC 3 cut(s) 239, 754, 959
PpsI GAGTC 3 cut(s) 239, 754, 959
Psp6I CCWGG 1 cut(s) 691
PspFI CCCAGC 1 cut(s) 831
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 1 cut(s) 837
PstI CTGCAG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 13, 27
RsaI GTAC 1 cut(s) 845
RsaNI GTAC 1 cut(s) 844
RseI CAYNNNNRTG 2 cut(s) 378, 485
SacII CCGCGG 1 cut(s) 130
SatI GCNGC 8 cut(s) 127, 130, 295, 298, 325, 618, 784, 980
Sau3AI GATC 5 cut(s) 13, 27, 630, 667, 853
SchI GAGTC 3 cut(s) 239, 755, 959
ScrFI CCNGG 3 cut(s) 403, 559, 693
SfaNI GCATC 1 cut(s) 862
SfcI CTRYAG 1 cut(s) 322
Sfr303I CCGCGG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 484
SgrBI CCGCGG 1 cut(s) 130
SmiMI CAYNNNNRTG 2 cut(s) 378, 485
Sse9I AATT 2 cut(s) 356, 794
SsiI CCGC 6 cut(s) 61, 115, 124, 127, 129, 295
SspMI CTAG 2 cut(s) 800, 828
StyD4I CCNGG 3 cut(s) 401, 557, 691
StyI CCWWGG 2 cut(s) 277, 679
TaqI TCGA 4 cut(s) 160, 484, 582, 852
TasI AATT 2 cut(s) 356, 794
TatI WGTACW 1 cut(s) 843
TauI GCSGC 3 cut(s) 129, 132, 297
TfiI GAWTC 2 cut(s) 410, 914
TscAI CASTG 3 cut(s) 89, 229, 632
TseFI GTSAC 1 cut(s) 456
TseI GCWGC 5 cut(s) 297, 324, 617, 783, 979
Tsp45I GTSAC 1 cut(s) 456
TspDTI ATGAA 2 cut(s) 230, 972
TspGWI ACGGA 2 cut(s) 690, 896
TspRI CASTG 3 cut(s) 89, 229, 632
Van91I CCANNNNNTGG 1 cut(s) 84
XapI RAATTY 1 cut(s) 356
XmiI GTMKAC 1 cut(s) 937
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 2 cut(s) 800, 828
Zsp2I ATGCAT 2 cut(s) 877, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.