Rh1DG147800

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002699
Physical Location & Seq
Forward (+)
30935590 .. 30937700
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG147800.1

Sequence Viewer

Length: 1104 bp
ATGTTCCACCAAGATGTCCTCACCCTCATCCTTCTACACCTCCCCGTCAAGTCTCTCCTCCGCTTCAAATGCGTCTCTAAGCAGTGGCTCTCTGTTATCTCCAGCCCCCAGTTCCACCACCGCCGCCGCCGTCTCGCCTCCTCCGGCATCATCTTGTGTCAAACAACCGATCTCATTCACCACATATCCCTCGCCAGAAGCAACTCCGACCCACCCTTCACATCCCTGAGCTTCATCGACGACTCGGCAGGTGTCAAGATCCTCCAGTCCAGCAATGGCCTGCTCTTGTGCTGCAGCAGCCTTCACAAAGTTGGTATACGCCGCAGCTATTACATCTGCAATCCTTCCACAAAGCAAGTCTCGACTCTTCCTGAAGCAGATTGCGAGTCCAAAACTATTTCTGGAGTTTACTTAGCTTTTGATTCGTCCAAGTCGCCTCACTACCAAGTTGTGTCTGTTCGAAGTTGTTCTTCCCCACCATCATCCAATAGCTACCGCATCGTTACTTACTCTTCCGAGACTCGGGCTTGGAGGCTTTCCGGGTCTCCTTTCGCTGCCCCAGACCTGGTGTTTGACAATGGAGTGCTTTGGAATGGTGCAATACACTGGATTAGTCCAACTGGGGATTCATTATGTTTTGACATCGATCAAGAACTCCTCGGAACATTGCCTAGTCTTCCATCAACTGATAAATGGGGCAACAGGAGGTTGGGGTATTTCGGCGAGTCCGGTGGCCATTTACACCTAATTGAAGTCTACGGCAGCGCGACTCAATTCCATGTCTTTGAGATGGAGAGAGACTACTCTAGCTGGCTTCCAAAGTACCAAGTTGACATAGCAGCGATTGTTGATGCATTTCCACTGATGGTTTGGAACTACCCTAACGCTAATGATTCTGTTTTTTATGCATTTAGTCTGCTATTTGTCAAAGAAGATGAAGAGGACTCAATTCTGTTGTTGCAAGTACCTGGTGAGTTTCTAACATATAATCTTGGCAATGGGACCTTCAAGAAGCTTGGTCCAATATCCACCACAACAAATATTGCATTTCAGATTGGATGTTTCCATGCATATCAGTTTACAGAAACTTTGGCTAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

367

Amino Acids

40.96

Weight (kDa)

6.34

Isoelectric Point (pI)

57.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 40 2.3e-08 F-box domain
FBA_3 PF08268 76 - 222 3.4e-11 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 78 - 290 1.6e-08 F-box protein At3g26010-like, beta-propeller
Beta-prop_KIB1-4 PF03478 80 - 272 4.1e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 239
AccI GTMKAC 2 cut(s) 316, 756
AccII CGCG 1 cut(s) 767
AciI CCGC 6 cut(s) 61, 121, 124, 127, 322, 496
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 733
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 2 cut(s) 824, 966
AfiI CCNNNNNNNGG 2 cut(s) 522, 565
AgsI TTSAA 3 cut(s) 67, 752, 1009
AjnI CCWGG 2 cut(s) 564, 967
AjuI GAANNNNNNNTTGG 2 cut(s) 610, 642
AluBI AGCT 6 cut(s) 231, 327, 416, 492, 810, 1015
AluI AGCT 6 cut(s) 231, 327, 416, 492, 810, 1015
Alw26I GTCTC 7 cut(s) 57, 79, 137, 364, 512, 549, 792
AlwI GGATC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 522
AoxI GGCC 2 cut(s) 277, 733
ApeKI GCWGC 7 cut(s) 291, 294, 297, 324, 554, 762, 839
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
Asp700I GAANNNNTTC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 2 cut(s) 1002, 1019
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 3 cut(s) 13, 170, 983
AsuII TTCGAA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 522
AvaII GGWCC 2 cut(s) 1002, 1019
BalI TGGCCA 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 668
BbvI GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
BccI CCATC 4 cut(s) 487, 688, 784, 859
BceAI ACGGC 2 cut(s) 114, 775
BciT130I CCWGG 2 cut(s) 566, 969
BcnI CCSGG 1 cut(s) 541
BcoDI GTCTC 7 cut(s) 57, 79, 137, 364, 512, 549, 792
BfaI CTAG 3 cut(s) 672, 807, 1095
BfmI CTRYAG 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 239
Bme1390I CCNGG 3 cut(s) 541, 566, 969
Bme18I GGWCC 2 cut(s) 1002, 1019
BmeT110I CYCGRG 1 cut(s) 522
BmgT120I GGNCC 2 cut(s) 1002, 1019
BmiI GGNNCC 1 cut(s) 1003
BmrFI CCNGG 3 cut(s) 541, 566, 969
BmrI ACTGGG 2 cut(s) 103, 630
BmsI GCATC 3 cut(s) 156, 507, 841
BmuI ACTGGG 2 cut(s) 103, 630
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 3 cut(s) 85, 248, 423
Bpu10I CCTNAGC 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 541
Bsa29I ATCGAT 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 658
BsaWI WCCGGW 1 cut(s) 728
Bsc4I CCNNNNNNNGG 2 cut(s) 522, 565
Bse1I ACTGG 4 cut(s) 109, 265, 611, 625
Bse3DI GCAATG 3 cut(s) 280, 665, 1003
BseBI CCWGG 2 cut(s) 566, 969
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 658
BseGI GGATG 4 cut(s) 27, 221, 482, 1064
BseLI CCNNNNNNNGG 2 cut(s) 522, 565
BseMI GCAATG 3 cut(s) 280, 665, 1003
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 4 cut(s) 109, 265, 611, 625
BseRI GAGGAG 3 cut(s) 47, 130, 647
BseXI GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
Bsh1236I CGCG 1 cut(s) 767
BshFI GGCC 2 cut(s) 279, 735
BshVI ATCGAT 1 cut(s) 645
BsiHKCI CYCGRG 1 cut(s) 522
BsiSI CCGG 3 cut(s) 144, 540, 729
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 2 cut(s) 522, 565
BsmAI GTCTC 7 cut(s) 57, 79, 137, 364, 512, 549, 792
BsmBI CGTCTC 2 cut(s) 79, 137
BsmFI GGGAC 1 cut(s) 1015
BsnI GGCC 2 cut(s) 279, 735
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 522
Bsp119I TTCGAA 1 cut(s) 460
Bsp143I GATC 3 cut(s) 169, 258, 646
BspACI CCGC 6 cut(s) 61, 121, 124, 127, 322, 496
BspANI GGCC 2 cut(s) 279, 735
BspCNI CTCAG 1 cut(s) 219
BspDI ATCGAT 1 cut(s) 645
BspFNI CGCG 1 cut(s) 767
BspLI GGNNCC 1 cut(s) 1003
BspMAI CTGCAG 1 cut(s) 296
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 3 cut(s) 280, 665, 1003
BsrI ACTGG 4 cut(s) 109, 265, 611, 625
BssECI CCNNGG 1 cut(s) 658
BssMI GATC 3 cut(s) 169, 258, 646
BssNAI GTATAC 1 cut(s) 317
Bst1107I GTATAC 1 cut(s) 317
Bst2UI CCWGG 2 cut(s) 566, 969
Bst6I CTCTTC 3 cut(s) 372, 517, 933
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 281, 812
BstDEI CTNAG 3 cut(s) 78, 227, 412
BstF5I GGATG 4 cut(s) 27, 221, 482, 1064
BstFNI CGCG 1 cut(s) 767
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 3 cut(s) 172, 261, 649
BstMAI GTCTC 7 cut(s) 57, 79, 137, 364, 512, 549, 792
BstMBI GATC 3 cut(s) 169, 258, 646
BstMWI GCNNNNNNNGC 2 cut(s) 69, 297
BstNI CCWGG 2 cut(s) 566, 969
BstSCI CCNGG 3 cut(s) 539, 564, 967
BstSFI CTRYAG 1 cut(s) 292
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
BstV2I GAAGAC 1 cut(s) 668
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BstZ17I GTATAC 1 cut(s) 317
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 2 cut(s) 279, 735
BsuTUI ATCGAT 1 cut(s) 645
BtsCI GGATG 4 cut(s) 27, 221, 482, 1064
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 3 cut(s) 89, 604, 860
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 2 cut(s) 281, 812
CfoI GCGC 1 cut(s) 767
Cfr13I GGNCC 2 cut(s) 1002, 1019
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 1 cut(s) 61
CsiI ACCWGGT 2 cut(s) 564, 967
Csp6I GTAC 2 cut(s) 823, 965
CviAII CATG 2 cut(s) 779, 1067
CviQI GTAC 2 cut(s) 823, 965
DdeI CTNAG 3 cut(s) 78, 227, 412
DpnI GATC 3 cut(s) 171, 260, 648
DpnII GATC 3 cut(s) 169, 258, 646
EaeI YGGCCR 1 cut(s) 733
Eam1104I CTCTTC 3 cut(s) 372, 517, 933
EarI CTCTTC 3 cut(s) 372, 517, 933
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 1002, 1019
Eco57I CTGAAG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 1002
EcoRII CCWGG 2 cut(s) 564, 967
EcoT22I ATGCAT 3 cut(s) 856, 910, 1072
Esp3I CGTCTC 2 cut(s) 79, 137
FaeI CATG 2 cut(s) 782, 1070
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 1 cut(s) 1015
FatI CATG 2 cut(s) 778, 1066
FblI GTMKAC 2 cut(s) 316, 756
FokI GGATG 4 cut(s) 14, 208, 469, 1071
FspBI CTAG 3 cut(s) 672, 807, 1095
GlaI GCGC 1 cut(s) 766
GsuI CTGGAG 3 cut(s) 85, 248, 423
HaeIII GGCC 2 cut(s) 279, 735
HapII CCGG 3 cut(s) 144, 540, 729
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 767
Hin1II CATG 2 cut(s) 782, 1070
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 1013
HpaII CCGG 3 cut(s) 144, 540, 729
HphI GGTGA 3 cut(s) 13, 170, 983
Hpy166II GTNNAC 5 cut(s) 317, 409, 757, 832, 1080
Hpy188I TCNGA 4 cut(s) 208, 517, 662, 1053
Hpy188III TCNNGA 6 cut(s) 256, 361, 371, 402, 650, 1009
Hpy8I GTNNAC 5 cut(s) 317, 409, 757, 832, 1080
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 5 cut(s) 41, 226, 311, 354, 1015
HpyCH4V TGCA 8 cut(s) 294, 339, 599, 854, 908, 961, 1046, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 297
HpyF3I CTNAG 3 cut(s) 78, 227, 412
Hsp92II CATG 2 cut(s) 782, 1070
HspAI GCGC 1 cut(s) 765
Kzo9I GATC 3 cut(s) 169, 258, 646
Lsp1109I GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
LweI GCATC 3 cut(s) 156, 507, 841
MabI ACCWGGT 2 cut(s) 564, 967
MaeI CTAG 3 cut(s) 672, 807, 1095
MaeIII GTNAC 1 cut(s) 502
MalI GATC 3 cut(s) 171, 260, 648
MboI GATC 3 cut(s) 169, 258, 646
MboII GAAGA 6 cut(s) 359, 462, 504, 668, 944, 950
MflI RGATCY 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 735
MluCI AATT 3 cut(s) 747, 773, 948
MluNI TGGCCA 1 cut(s) 735
MlyI GAGTC 7 cut(s) 236, 358, 395, 514, 734, 763, 938
MmeI TCCRAC 2 cut(s) 231, 641
Mox20I TGGCCA 1 cut(s) 735
Mph1103I ATGCAT 3 cut(s) 856, 910, 1072
MroXI GAANNNNTTC 1 cut(s) 466
MscI TGGCCA 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 12
Msp20I TGGCCA 1 cut(s) 735
MspI CCGG 3 cut(s) 144, 540, 729
MspR9I CCNGG 3 cut(s) 541, 566, 969
MvaI CCWGG 2 cut(s) 566, 969
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 2 cut(s) 69, 297
NciI CCSGG 1 cut(s) 541
NdeII GATC 3 cut(s) 169, 258, 646
NlaIII CATG 2 cut(s) 782, 1070
NlaIV GGNNCC 1 cut(s) 1003
NmeAIII GCCGAG 1 cut(s) 224
NsiI ATGCAT 3 cut(s) 856, 910, 1072
NspV TTCGAA 1 cut(s) 460
PaqCI CACCTGC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 3 cut(s) 422, 626, 893
PleI GAGTC 7 cut(s) 236, 358, 394, 514, 733, 763, 938
PpsI GAGTC 7 cut(s) 236, 358, 394, 514, 733, 763, 938
PpuMI RGGWCCY 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 1002
Psp6I CCWGG 2 cut(s) 564, 967
PspGI CCWGG 2 cut(s) 564, 967
PspN4I GGNNCC 1 cut(s) 1003
PspPI GGNCC 2 cut(s) 1002, 1019
PspPPI RGGWCCY 1 cut(s) 1002
PstI CTGCAG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 2 cut(s) 824, 966
RsaNI GTAC 2 cut(s) 823, 965
RseI CAYNNNNRTG 1 cut(s) 12
Sau3AI GATC 3 cut(s) 169, 258, 646
Sau96I GGNCC 2 cut(s) 1002, 1019
SchI GAGTC 7 cut(s) 236, 358, 395, 514, 734, 763, 938
ScrFI CCNGG 3 cut(s) 541, 566, 969
SexAI ACCWGGT 2 cut(s) 564, 967
SfaNI GCATC 3 cut(s) 156, 507, 841
SfcI CTRYAG 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 2 cut(s) 1002, 1019
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 3 cut(s) 747, 773, 948
SsiI CCGC 6 cut(s) 61, 121, 124, 127, 322, 496
SspI AATATT 1 cut(s) 1042
SspMI CTAG 3 cut(s) 672, 807, 1095
StyD4I CCNGG 3 cut(s) 539, 564, 967
TaqI TCGA 4 cut(s) 237, 362, 460, 645
TasI AATT 3 cut(s) 747, 773, 948
TauI GCSGC 3 cut(s) 126, 129, 324
TfiI GAWTC 3 cut(s) 422, 626, 893
TscAI CASTG 3 cut(s) 89, 611, 867
TseI GCWGC 7 cut(s) 291, 294, 297, 324, 554, 762, 839
TspDTI ATGAA 3 cut(s) 223, 618, 951
TspRI CASTG 3 cut(s) 89, 611, 867
VpaK11BI GGWCC 2 cut(s) 1002, 1019
XcmI CCANNNNNNNNNTGG 2 cut(s) 272, 867
XmiI GTMKAC 2 cut(s) 316, 756
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 3 cut(s) 672, 807, 1095
Zsp2I ATGCAT 3 cut(s) 856, 910, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.