Rh4AG082300

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002699
Physical Location & Seq
Reverse (-)
17177621 .. 17179162
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG082300.1

Sequence Viewer

Length: 1182 bp
ATGCTGCTGCTTCTCTGGGATCCACTGCCTCCAGCAGAAGCAGTTGCTGGCAACCAAGATCTCATCACAGACATCCTTCTACGCCTCCCTGTCAAGCCTCTTCTCCGGTTCAAATGCGTCTCCAAGCAGTGGCTCTCTCTTATCTCCGACCCCCGATTCAGTCGTAATTATCGCAGTCTTGCATCTGCCAGCATCCTGTTGTGCGGAACAACCGAGCTCATTGATCATGTATCTTGCGGCAGAAGAAACTCCGAGCCGCCCTTTACTTCTCTCGGTTTCATTAGTGACTCTGCAGGTACCAAAATCTTGCAGTCATGCAATGGCTTGATGTTGTGTTGCAGCTTTTTCAAAATGGGAAAGAGTTGCAACTATTACATCTGTAATCCTTCAACAAGGCAATTCTTGATGCTTCCTCCACCTAATGCCAAAGGGTGTGATGAATCCACAACCATTCTTGGTGTTTATTTAGCTTTTGATCCTTCTAAATCACCTCACTACCAAGTTGTCTGTGTTCGAAATTGTTCTTCCTCGTCAGCAGCAGCAGCGAATGATAATTACCAGATTGAAATATACTCGTCTGAGACTCTCAGTTGGAGGCTTTCCGGGTCTCCTTTCAGTGCTCCATTCGACATGGTTTTTGACAACGGGGTTTTTTGGAATGGTACAATACATTGGATTAGTCCAACAGGGGCTTCACTCTGTTTTGATATTGTCCACGAGCAACTTGGAGAAATGCCTAGCCTTCCATCAAATGAGAAATGGAGCAAGAGGAGGTTAAGGTATTTCGGCGAGTCTAGTGGTCATTTGCACCTAATTGAAATCTATGGCTCTGGCACCACTCAATTCCAAGTCTTCGAGATGAAGAAAGACTACTCTAGCTGGATTCCCAAGTACCAAGTTGACCTCACCGCGATTGTCAAGGCATTTCCAGAGATGGTTCGGAGCTACCCTGACGCCCATGATTCTCGATTTTGTCTATTTAGTATCCTCTTTGTGCAAGAGAACGAAGAGGACTCATTATTGTTGCTGCAAATCCCTGGTAAGTTCATATCTTACAATCTTAGACATAAGACCTTCAAGGAGATTTGTGATTTTGGAGCAAACTCCACCAAGGCAAATACTTCATTACCGATTGGTTGCTTCCAAGCTTATCAGTTCATAGAGACTCTGGCTTGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

393

Amino Acids

44.13

Weight (kDa)

6.45

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 54 4.7e-07 F-box domain
b-prop_At3g26010-like PF24750 62 - 310 7.2e-12 F-box protein At3g26010-like, beta-propeller
Beta-prop_KIB1-4 PF03478 96 - 294 1.6e-10 KIB1-4 beta-propeller
FBA_3 PF08268 98 - 252 1.3e-12 F-box associated beta propeller domain
FBA_1 PF07734 103 - 262 1.5e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
Acc65I GGTACC 1 cut(s) 296
AccB1I GGYRCC 2 cut(s) 296, 833
AccB7I CCANNNNNTGG 1 cut(s) 129
AccII CGCG 1 cut(s) 911
AciI CCGC 4 cut(s) 204, 237, 257, 909
AclWI GGATC 3 cut(s) 14, 27, 470
AcyI GRCGYC 1 cut(s) 954
AfaI GTAC 3 cut(s) 298, 664, 893
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 6 cut(s) 112, 349, 390, 566, 818, 1078
AjnI CCWGG 1 cut(s) 1036
AjuI GAANNNNNNNTTGG 2 cut(s) 676, 708
AluBI AGCT 6 cut(s) 217, 342, 470, 879, 945, 1148
AluI AGCT 6 cut(s) 217, 342, 470, 879, 945, 1148
Alw21I GWGCWC 2 cut(s) 219, 622
Alw26I GTCTC 4 cut(s) 124, 575, 612, 1157
AlwI GGATC 3 cut(s) 14, 27, 470
AlwNI CAGNNNCTG 1 cut(s) 47
ApeKI GCWGC 7 cut(s) 4, 7, 339, 536, 539, 542, 1027
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
Asp700I GAANNNNTTC 1 cut(s) 520
Asp718I GGTACC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 2 cut(s) 480, 898
AsuII TTCGAA 1 cut(s) 514
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 2 cut(s) 296, 833
BanII GRGCYC 1 cut(s) 219
BarI GAAGNNNNNNTAC 2 cut(s) 854, 886
BauI CACGAG 1 cut(s) 716
BbsI GAAGAC 1 cut(s) 844
Bbv12I GWGCWC 2 cut(s) 219, 622
BbvI GCAGC 5 cut(s) 351, 548, 551, 554, 1014
BccI CCATC 2 cut(s) 754, 928
BciT130I CCWGG 1 cut(s) 1038
BciVI GTATCC 1 cut(s) 995
BclI TGATCA 1 cut(s) 223
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 4 cut(s) 124, 575, 612, 1157
BfaI CTAG 3 cut(s) 738, 795, 876
BfmI CTRYAG 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 284
BfuI GTATCC 1 cut(s) 995
BglII AGATCT 1 cut(s) 58
BisI GCNGC 9 cut(s) 5, 8, 238, 257, 340, 537, 540, 543, 1028
BlsI GCNGC 9 cut(s) 6, 9, 239, 258, 341, 538, 541, 544, 1029
Bme1390I CCNGG 2 cut(s) 604, 1038
BmiI GGNNCC 3 cut(s) 21, 298, 835
BmrFI CCNGG 2 cut(s) 604, 1038
BmsI GCATC 3 cut(s) 191, 201, 396
BpiI GAAGAC 1 cut(s) 844
BpmI CTGGAG 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 514
BpuMI CCSGG 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 954
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 2 cut(s) 1036, 1110
BsaWI WCCGGW 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse3DI GCAATG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 1038
BseDI CCNNGG 2 cut(s) 1036, 1110
BseGI GGATG 2 cut(s) 72, 192
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 570, 601
BseRI GAGGAG 1 cut(s) 784
BseXI GCAGC 5 cut(s) 351, 548, 551, 554, 1014
Bsh1236I CGCG 1 cut(s) 911
BshNI GGYRCC 2 cut(s) 296, 833
BsiHKAI GWGCWC 2 cut(s) 219, 622
BsiSI CCGG 2 cut(s) 106, 603
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 4 cut(s) 124, 575, 612, 1157
BsmBI CGTCTC 1 cut(s) 124
Bso31I GGTCTC 1 cut(s) 612
Bsp119I TTCGAA 1 cut(s) 514
Bsp1286I GDGCHC 2 cut(s) 219, 622
Bsp143I GATC 4 cut(s) 19, 58, 223, 475
BspACI CCGC 4 cut(s) 204, 237, 257, 909
BspCNI CTCAG 2 cut(s) 571, 600
BspFNI CGCG 1 cut(s) 911
BspLI GGNNCC 3 cut(s) 21, 298, 835
BspMAI CTGCAG 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 3 cut(s) 14, 27, 470
BspT104I TTCGAA 1 cut(s) 514
BspT107I GGYRCC 2 cut(s) 296, 833
BspTNI GGTCTC 1 cut(s) 612
BsrDI GCAATG 1 cut(s) 325
BssECI CCNNGG 2 cut(s) 1036, 1110
BssMI GATC 4 cut(s) 19, 58, 223, 475
BssNI GRCGYC 1 cut(s) 954
BssSI CACGAG 1 cut(s) 716
BssT1I CCWWGG 1 cut(s) 1110
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 1 cut(s) 1038
Bst6I CTCTTC 2 cut(s) 105, 1002
BstACI GRCGYC 1 cut(s) 954
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 2 cut(s) 49, 190
BstDEI CTNAG 3 cut(s) 579, 587, 1061
BstF5I GGATG 2 cut(s) 72, 192
BstFNI CGCG 1 cut(s) 911
BstKTI GATC 4 cut(s) 22, 61, 226, 478
BstMAI GTCTC 4 cut(s) 124, 575, 612, 1157
BstMBI GATC 4 cut(s) 19, 58, 223, 475
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 1038
BstSCI CCNGG 2 cut(s) 602, 1036
BstSFI CTRYAG 1 cut(s) 291
BstUI CGCG 1 cut(s) 911
BstV1I GCAGC 5 cut(s) 351, 548, 551, 554, 1014
BstV2I GAAGAC 1 cut(s) 844
BstX2I RGATCY 2 cut(s) 19, 58
BstYI RGATCY 2 cut(s) 19, 58
BsuI GTATCC 1 cut(s) 995
BtsCI GGATG 2 cut(s) 72, 192
BtsI GCAGTG 2 cut(s) 23, 134
BtsIMutI CAGTG 3 cut(s) 23, 134, 622
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 49, 190
CaiI CAGNNNCTG 1 cut(s) 47
CseI GACGC 2 cut(s) 106, 962
Csp6I GTAC 3 cut(s) 297, 663, 892
CviAII CATG 4 cut(s) 227, 315, 631, 959
CviQI GTAC 3 cut(s) 297, 663, 892
DdeI CTNAG 3 cut(s) 579, 587, 1061
DpnI GATC 4 cut(s) 21, 60, 225, 477
DpnII GATC 4 cut(s) 19, 58, 223, 475
Eam1104I CTCTTC 2 cut(s) 105, 1002
EarI CTCTTC 2 cut(s) 105, 1002
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 1110
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 612
Eco53kI GAGCTC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 1036
EcoT14I CCWWGG 1 cut(s) 1110
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 1110
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 4 cut(s) 230, 318, 634, 962
FaiI YATR 9 cut(s) 228, 316, 571, 632, 825, 960, 1049, 1068, 1160
FatI CATG 4 cut(s) 226, 314, 630, 958
FbaI TGATCA 1 cut(s) 223
Fnu4HI GCNGC 9 cut(s) 5, 8, 238, 257, 340, 537, 540, 543, 1028
FokI GGATG 2 cut(s) 59, 179
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 9 cut(s) 5, 8, 238, 257, 340, 537, 540, 543, 1028
FspBI CTAG 3 cut(s) 738, 795, 876
GluI GCNGC 9 cut(s) 5, 8, 238, 257, 340, 537, 540, 543, 1028
GsuI CTGGAG 1 cut(s) 15
HapII CCGG 2 cut(s) 106, 603
HgaI GACGC 2 cut(s) 106, 962
Hin1I GRCGYC 1 cut(s) 954
Hin1II CATG 4 cut(s) 230, 318, 634, 962
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HindIII AAGCTT 1 cut(s) 1146
HinfI GANTC 9 cut(s) 156, 287, 440, 583, 791, 883, 962, 1013, 1165
HpaII CCGG 2 cut(s) 106, 603
HphI GGTGA 2 cut(s) 480, 898
Hpy166II GTNNAC 2 cut(s) 715, 901
Hpy188I TCNGA 4 cut(s) 148, 253, 580, 942
Hpy188III TCNNGA 4 cut(s) 403, 856, 929, 966
Hpy8I GTNNAC 2 cut(s) 715, 901
HpyAV CCTTC 5 cut(s) 86, 396, 489, 752, 1084
HpyCH4V TGCA 9 cut(s) 182, 293, 310, 318, 339, 366, 808, 997, 1030
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 3 cut(s) 579, 587, 1061
Hsp92I GRCGYC 1 cut(s) 954
Hsp92II CATG 4 cut(s) 230, 318, 634, 962
KpnI GGTACC 1 cut(s) 300
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 4 cut(s) 19, 58, 223, 475
LmnI GCTCC 4 cut(s) 625, 762, 942, 1097
Lsp1109I GCAGC 5 cut(s) 351, 548, 551, 554, 1014
LweI GCATC 3 cut(s) 191, 201, 396
MaeI CTAG 3 cut(s) 738, 795, 876
MaeIII GTNAC 1 cut(s) 284
MalI GATC 4 cut(s) 21, 60, 225, 477
MboI GATC 4 cut(s) 19, 58, 223, 475
MboII GAAGA 6 cut(s) 92, 255, 516, 844, 874, 1019
MflI RGATCY 2 cut(s) 19, 58
MhlI GDGCHC 2 cut(s) 219, 622
MluCI AATT 6 cut(s) 166, 398, 517, 553, 813, 842
MlyI GAGTC 5 cut(s) 281, 577, 800, 1007, 1159
MmeI TCCRAC 3 cut(s) 171, 572, 707
MroXI GAANNNNTTC 1 cut(s) 520
MseI TTAA 1 cut(s) 776
MspI CCGG 2 cut(s) 106, 603
MspR9I CCNGG 2 cut(s) 604, 1038
MvaI CCWGG 1 cut(s) 1038
MvnI CGCG 1 cut(s) 911
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 1 cut(s) 604
NdeII GATC 4 cut(s) 19, 58, 223, 475
NlaIII CATG 4 cut(s) 230, 318, 634, 962
NlaIV GGNNCC 3 cut(s) 21, 298, 835
NmuCI GTSAC 1 cut(s) 284
NspV TTCGAA 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 4 cut(s) 156, 440, 883, 962
PflMI CCANNNNNTGG 1 cut(s) 129
PkrI GCNGC 9 cut(s) 6, 9, 239, 258, 341, 538, 541, 544, 1029
PleI GAGTC 5 cut(s) 281, 577, 799, 1007, 1159
PpsI GAGTC 5 cut(s) 281, 577, 799, 1007, 1159
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 1036
PspGI CCWGG 1 cut(s) 1036
PspN4I GGNNCC 3 cut(s) 21, 298, 835
PstI CTGCAG 1 cut(s) 295
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 2 cut(s) 19, 58
RsaI GTAC 3 cut(s) 298, 664, 893
RsaNI GTAC 3 cut(s) 297, 663, 892
SacI GAGCTC 1 cut(s) 219
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 9 cut(s) 5, 8, 238, 257, 340, 537, 540, 543, 1028
Sau3AI GATC 4 cut(s) 19, 58, 223, 475
SchI GAGTC 5 cut(s) 281, 577, 800, 1007, 1159
ScrFI CCNGG 2 cut(s) 604, 1038
SduI GDGCHC 2 cut(s) 219, 622
SfaNI GCATC 3 cut(s) 191, 201, 396
SfcI CTRYAG 1 cut(s) 291
SfuI TTCGAA 1 cut(s) 514
Sse9I AATT 6 cut(s) 166, 398, 517, 553, 813, 842
SsiI CCGC 4 cut(s) 204, 237, 257, 909
SspMI CTAG 3 cut(s) 738, 795, 876
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 602, 1036
StyI CCWWGG 1 cut(s) 1110
TaqI TCGA 4 cut(s) 514, 627, 855, 967
TasI AATT 6 cut(s) 166, 398, 517, 553, 813, 842
TauI GCSGC 2 cut(s) 240, 259
TfiI GAWTC 4 cut(s) 156, 440, 883, 962
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 3 cut(s) 30, 134, 622
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 7 cut(s) 4, 7, 339, 536, 539, 542, 1027
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 6 cut(s) 268, 453, 875, 1036, 1113, 1147
TspRI CASTG 3 cut(s) 30, 134, 622
Van91I CCANNNNNTGG 1 cut(s) 129
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 3 cut(s) 738, 795, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.