FvH4_4g12520

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003947
Physical Location & Seq
Forward (+)
16201931 .. 16203691
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12520.t2

Sequence Viewer

Length: 759 bp
ATGTCAGCTGCCGTGGAAGCTGTGTCTGCAACCAAAGATGTTGGTTTCACTTCTCAAGTGCAGGAAGTAGAGTTTAAGAATGATAAGAACCGAAACAAGGATGAAGGTGGAAAACCAAGCCTGCATCACCGTGATGAAAATGATGAACTTAAAGATGAGGTTGATCCAGATGAAGAGGATGAGCTTGCCACACTGAAATGTGACAAAGAATTGGCTATTGGCCCCAAGTTTTCGCTCAAGGAGCAGCTTGATAAGGATAAAGATGATGAGAGTTTAAGGAAGTGGAAGCAGCAACTTCTTGGGAGTGTTGATCTCTCTGCACTAGCTGTTGGAGAGAGTAAAGAAGCAGAAGTGAGGATTCTAAGCCTGACAATGATGTTCCGGGATCGGCCTGAACTTGTGCTGCCGATTCCATTCACCAACAAACCAAAGAGCAGTCTTTTCACACTCAAAGGAGGAAGCAAGTACCGGACTAAATTCACATTCATTGTCTCTAAAAACATAGTTTCAGGCCTCATGTACACCACCTCAGTCTGGAAGACTGGTGTTAAAGTGCACAATTCAAAAAAAATGCTTGGAACCTTTAGTCCTCGACAAGATCCATATACGTATGAAACCGAAGAAGATACTACCCCTTCAGGCATGTTTGCAAGGGGCTGGTATTTTGTGAGAACAAAATTTCTGGATGATGATGGAAAATGCTATTTGGATGCCAGTTACTACTTTGAAATCCAGAAGAACTGGGCAACACCCTCTTGA

Protein Analysis

253

Amino Acids

28.71

Weight (kDa)

5.46

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 59 - 184 7.5e-40 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 158, 393, 593
AcsI RAATTY 2 cut(s) 476, 677
AcuI CTGAAG 1 cut(s) 621
AfaI GTAC 2 cut(s) 467, 521
AfiI CCNNNNNNNGG 2 cut(s) 97, 534
AgsI TTSAA 2 cut(s) 564, 728
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AloI GAACNNNNNNTCC 2 cut(s) 571, 603
AluBI AGCT 5 cut(s) 8, 20, 184, 247, 326
AluI AGCT 5 cut(s) 8, 20, 184, 247, 326
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 1 cut(s) 496
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 3 cut(s) 158, 393, 593
AoxI GGCC 3 cut(s) 220, 389, 511
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 4 cut(s) 8, 244, 289, 403
ApoI RAATTY 2 cut(s) 476, 677
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 119, 409
BaeGI GKGCMC 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 3 cut(s) 256, 301, 390
BccI CCATC 1 cut(s) 686
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 1 cut(s) 323
BisI GCNGC 4 cut(s) 9, 245, 290, 404
BlsI GCNGC 4 cut(s) 10, 246, 291, 405
Bme1390I CCNGG 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 223, 580
BmrFI CCNGG 1 cut(s) 383
BmrI ACTGGG 1 cut(s) 751
BmsI GCATC 2 cut(s) 133, 700
BmuI ACTGGG 1 cut(s) 751
BpiI GAAGAC 1 cut(s) 545
BpuEI CTTGAG 2 cut(s) 39, 221
BpuMI CCSGG 1 cut(s) 383
BsaAI YACGTR 1 cut(s) 609
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 468
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 534
Bse1I ACTGG 3 cut(s) 547, 714, 746
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 4 cut(s) 106, 184, 691, 715
BseLI CCNNNNNNNGG 2 cut(s) 97, 534
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 3 cut(s) 547, 714, 746
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 3 cut(s) 256, 301, 390
BsgI GTGCAG 2 cut(s) 80, 303
BshFI GGCC 3 cut(s) 222, 391, 513
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 2 cut(s) 382, 469
BslI CCNNNNNNNGG 2 cut(s) 97, 534
BsmAI GTCTC 1 cut(s) 496
BsnI GGCC 3 cut(s) 222, 391, 513
Bsp1286I GDGCHC 1 cut(s) 558
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 4 cut(s) 163, 310, 385, 598
BspANI GGCC 3 cut(s) 222, 391, 513
BspCNI CTCAG 1 cut(s) 542
BspLI GGNNCC 2 cut(s) 223, 580
BspPI GGATC 3 cut(s) 158, 393, 593
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 3 cut(s) 547, 714, 746
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 4 cut(s) 163, 310, 385, 598
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 168
BstAUI TGTACA 1 cut(s) 519
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 122, 186
BstDEI CTNAG 2 cut(s) 362, 529
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 4 cut(s) 106, 184, 691, 715
BstKTI GATC 4 cut(s) 166, 313, 388, 601
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 4 cut(s) 163, 310, 385, 598
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 241
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 558
BstSNI TACGTA 1 cut(s) 609
BstV1I GCAGC 3 cut(s) 256, 301, 390
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 3 cut(s) 222, 391, 513
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 4 cut(s) 106, 184, 691, 715
BtsIMutI CAGTG 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 122, 186
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 466, 520
CviAII CATG 2 cut(s) 517, 643
CviQI GTAC 2 cut(s) 466, 520
DdeI CTNAG 2 cut(s) 362, 529
DpnI GATC 4 cut(s) 165, 312, 387, 600
DpnII GATC 4 cut(s) 163, 310, 385, 598
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco105I TACGTA 1 cut(s) 609
Eco147I AGGCCT 1 cut(s) 513
Eco57I CTGAAG 1 cut(s) 621
FaeI CATG 2 cut(s) 520, 646
FaiI YATR 6 cut(s) 503, 518, 604, 606, 612, 644
FatI CATG 2 cut(s) 516, 642
Fnu4HI GCNGC 4 cut(s) 9, 245, 290, 404
FokI GGATG 4 cut(s) 113, 191, 698, 722
Fsp4HI GCNGC 4 cut(s) 9, 245, 290, 404
FspBI CTAG 1 cut(s) 323
GluI GCNGC 4 cut(s) 9, 245, 290, 404
HaeIII GGCC 3 cut(s) 222, 391, 513
HapII CCGG 2 cut(s) 382, 469
Hin1II CATG 2 cut(s) 520, 646
HinfI GANTC 2 cut(s) 358, 409
HpaII CCGG 2 cut(s) 382, 469
HphI GGTGA 2 cut(s) 119, 409
Hpy166II GTNNAC 2 cut(s) 522, 556
Hpy188III TCNNGA 5 cut(s) 167, 535, 683, 733, 756
Hpy8I GTNNAC 2 cut(s) 522, 556
HpyAV CCTTC 2 cut(s) 98, 645
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 608
HpyCH4V TGCA 6 cut(s) 29, 61, 124, 320, 556, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 241
HpyF3I CTNAG 2 cut(s) 362, 529
HpySE526I ACGT 1 cut(s) 608
Hsp92II CATG 2 cut(s) 520, 646
Kzo9I GATC 4 cut(s) 163, 310, 385, 598
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 3 cut(s) 256, 301, 390
LweI GCATC 2 cut(s) 133, 700
MaeI CTAG 1 cut(s) 323
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 200, 716
MalI GATC 4 cut(s) 165, 312, 387, 600
MboI GATC 4 cut(s) 163, 310, 385, 598
MboII GAAGA 5 cut(s) 185, 550, 632, 635, 748
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 4 cut(s) 209, 476, 559, 677
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 7 cut(s) 151, 169, 348, 449, 524, 538, 600
MseI TTAA 4 cut(s) 75, 150, 275, 549
MslI CAYNNNNRTG 3 cut(s) 129, 132, 196
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 382, 469
MspR9I CCNGG 1 cut(s) 383
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 241
NciI CCSGG 1 cut(s) 383
NdeII GATC 4 cut(s) 163, 310, 385, 598
NlaIII CATG 2 cut(s) 520, 646
NlaIV GGNNCC 2 cut(s) 223, 580
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 646
PceI AGGCCT 1 cut(s) 513
PfeI GAWTC 2 cut(s) 358, 409
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 4 cut(s) 10, 246, 291, 405
Ppu21I YACGTR 1 cut(s) 609
PspN4I GGNNCC 2 cut(s) 223, 580
PspPI GGNCC 1 cut(s) 221
PsuI RGATCY 1 cut(s) 598
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 467, 521
RsaNI GTAC 2 cut(s) 466, 520
RseI CAYNNNNRTG 3 cut(s) 129, 132, 196
SaqAI TTAA 4 cut(s) 75, 150, 275, 549
SatI GCNGC 4 cut(s) 9, 245, 290, 404
Sau3AI GATC 4 cut(s) 163, 310, 385, 598
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 2 cut(s) 133, 700
SmiMI CAYNNNNRTG 3 cut(s) 129, 132, 196
SmlI CTYRAG 2 cut(s) 54, 236
SmoI CTYRAG 2 cut(s) 54, 236
SnaBI TACGTA 1 cut(s) 609
Sse9I AATT 4 cut(s) 209, 476, 559, 677
SseBI AGGCCT 1 cut(s) 513
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 1 cut(s) 611
TaqI TCGA 1 cut(s) 592
TasI AATT 4 cut(s) 209, 476, 559, 677
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 2 cut(s) 358, 409
Tru1I TTAA 4 cut(s) 75, 150, 275, 549
Tru9I TTAA 4 cut(s) 75, 150, 275, 549
TscAI CASTG 1 cut(s) 198
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 4 cut(s) 8, 244, 289, 403
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 6 cut(s) 117, 150, 159, 186, 475, 627
TspRI CASTG 1 cut(s) 198
VneI GTGCAC 1 cut(s) 554
XapI RAATTY 2 cut(s) 476, 677
XceI RCATGY 1 cut(s) 646
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.