Rh4BG163700

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003947
Physical Location & Seq
Forward (+)
29586333 .. 29592624
6292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG163700.1

Sequence Viewer

Length: 765 bp
ATGTCTGTAACCAAAGATGTTGGTTCCAGTTCTCAAGTGCAGGAAGTGGAGTTTAAGAATGATAAGAACAGCAACAAGGATGCAGGTGGTGAACCAAGCCTGCATCAACGTGACGAAAATGATAAGCTTAAAGATGAGGTTGATCCAGATGAAGAGGATGAGCTTGCCACATTCAAATGTGACAAAGAATTGGTTCTTGGCCCAAAGTTTTCTCCCAAGGAGCAGCTTGAGAAGGATCAAGATGATGAGAGTTTGAGGAAATGGAAGCAGCAACTTCTTGGGAGAGTTGATCTCTCTGCACTAGTTGGTGGAGAGAGTAAAGAAGCAGAAGTGAGGATTTTAAGTCAGACACTGATGTTCCAGGAGCGGCCTGAACTTGTGCTACCGATTCCATTCACCAACAAACCAAAGAGCAATCTTTTCACACTCAAAGGAGGAAGCAAGTTCCGGACCAAATTCACATTCACTGTCTCAAAAAACATTGTCTCAGGCCTCATGTCCACCGCCTCAGTCTGGAAGACTGGTGTCAAAGTGCACAGTTCAAAAAGAATGCTTGGAACCTTTAGCCCTCGACCAGATCCTTATACGTATGAAACTGAAGAAGAAACTACCCCTTCAGGCATGCTTGCAAGGGGCAGGTATTTTGTGAAAACAAAAAATATGACAAGAAACTGGAAAGGATCCAACATTGCTGAAGAAAAGACTAGTCAGAGCAGCAAGGATCAAGTATATGAATTCTCTAATTCCAATAACTCACGTTCATAA

Protein Analysis

254

Amino Acids

28.72

Weight (kDa)

6.79

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 53 - 219 6e-57 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 2 cut(s) 74, 627
AccBSI CCGCTC 1 cut(s) 367
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 2 cut(s) 367, 504
AclWI GGATC 6 cut(s) 137, 243, 572, 675, 688, 729
AcsI RAATTY 2 cut(s) 455, 734
AcuI CTGAAG 3 cut(s) 600, 618, 714
AfiI CCNNNNNNNGG 1 cut(s) 513
AgsI TTSAA 2 cut(s) 175, 543
AhlI ACTAGT 2 cut(s) 301, 704
AjnI CCWGG 1 cut(s) 360
AjuI GAANNNNNNNTTGG 4 cut(s) 180, 212, 446, 478
AluBI AGCT 3 cut(s) 127, 163, 226
AluI AGCT 3 cut(s) 127, 163, 226
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 2 cut(s) 475, 490
Alw44I GTGCAC 1 cut(s) 533
AlwI GGATC 6 cut(s) 137, 243, 572, 675, 688, 729
AlwNI CAGNNNCTG 1 cut(s) 352
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 3 cut(s) 199, 368, 490
ApaLI GTGCAC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 223, 268, 714
ApoI RAATTY 2 cut(s) 455, 734
Asp700I GAANNNNTTC 1 cut(s) 192
AspS9I GGNCC 2 cut(s) 200, 450
AsuHPI GGTGA 2 cut(s) 101, 388
AvaII GGWCC 1 cut(s) 450
BaeGI GKGCMC 1 cut(s) 537
BamHI GGATCC 1 cut(s) 680
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 3 cut(s) 235, 280, 726
BciT130I CCWGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 475, 490
BcuI ACTAGT 2 cut(s) 301, 704
BfaI CTAG 2 cut(s) 302, 705
BfuAI ACCTGC 2 cut(s) 74, 627
BisI GCNGC 4 cut(s) 224, 269, 368, 715
BlsI GCNGC 4 cut(s) 225, 270, 369, 716
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 2 cut(s) 200, 450
BmiI GGNNCC 3 cut(s) 25, 559, 682
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 2 cut(s) 70, 112
BoxI GACNNNNGTC 1 cut(s) 524
BpiI GAAGAC 1 cut(s) 524
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpuEI CTTGAG 2 cut(s) 18, 248
BsaAI YACGTR 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 447
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse1I ACTGG 3 cut(s) 27, 526, 677
Bse3DI GCAATG 1 cut(s) 687
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 85, 163
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 2 cut(s) 501, 522
BseNI ACTGG 3 cut(s) 27, 526, 677
BseSI GKGCMC 1 cut(s) 537
BseXI GCAGC 3 cut(s) 235, 280, 726
BsgI GTGCAG 2 cut(s) 59, 282
BshFI GGCC 3 cut(s) 201, 370, 492
BsiHKAI GWGCWC 1 cut(s) 537
BsiSI CCGG 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 2 cut(s) 475, 490
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 3 cut(s) 201, 370, 492
Bsp1286I GDGCHC 1 cut(s) 537
Bsp13I TCCGGA 1 cut(s) 447
Bsp143I GATC 6 cut(s) 142, 235, 289, 577, 680, 721
BspACI CCGC 2 cut(s) 367, 504
BspANI GGCC 3 cut(s) 201, 370, 492
BspCNI CTCAG 2 cut(s) 500, 521
BspEI TCCGGA 1 cut(s) 447
BspLI GGNNCC 3 cut(s) 25, 559, 682
BspMI ACCTGC 2 cut(s) 74, 627
BspPI GGATC 6 cut(s) 137, 243, 572, 675, 688, 729
BsrBI CCGCTC 1 cut(s) 367
BsrDI GCAATG 1 cut(s) 687
BsrI ACTGG 3 cut(s) 27, 526, 677
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 6 cut(s) 142, 235, 289, 577, 680, 721
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 469, 539
Bst6I CTCTTC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 588
BstC8I GCNNGC 4 cut(s) 101, 165, 623, 627
BstDEI CTNAG 2 cut(s) 487, 508
BstF5I GGATG 2 cut(s) 85, 163
BstKTI GATC 6 cut(s) 145, 238, 292, 580, 683, 724
BstMAI GTCTC 2 cut(s) 475, 490
BstMBI GATC 6 cut(s) 142, 235, 289, 577, 680, 721
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 625
BstPAI GACNNNNGTC 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 537
BstSNI TACGTA 1 cut(s) 588
BstV1I GCAGC 3 cut(s) 235, 280, 726
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 577, 680
BstYI RGATCY 2 cut(s) 577, 680
BsuRI GGCC 3 cut(s) 201, 370, 492
BtsCI GGATG 2 cut(s) 85, 163
BtsIMutI CAGTG 2 cut(s) 350, 465
BveI ACCTGC 2 cut(s) 74, 627
Cac8I GCNNGC 4 cut(s) 101, 165, 623, 627
CaiI CAGNNNCTG 1 cut(s) 352
Cfr13I GGNCC 2 cut(s) 200, 450
CviAII CATG 2 cut(s) 496, 622
CviJI RGCY 8 cut(s) 99, 127, 163, 201, 226, 370, 492, 567
CviKI_1 RGCY 8 cut(s) 99, 127, 163, 201, 226, 370, 492, 567
DdeI CTNAG 2 cut(s) 487, 508
DpnI GATC 6 cut(s) 144, 237, 291, 579, 682, 723
DpnII GATC 6 cut(s) 142, 235, 289, 577, 680, 721
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco105I TACGTA 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 3 cut(s) 600, 618, 714
EcoRI GAATTC 1 cut(s) 734
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 499, 625
FaiI YATR 8 cut(s) 497, 585, 591, 623, 662, 730, 732, 763
FatI CATG 2 cut(s) 495, 621
Fnu4HI GCNGC 4 cut(s) 224, 269, 368, 715
FokI GGATG 2 cut(s) 92, 170
Fsp4HI GCNGC 4 cut(s) 224, 269, 368, 715
FspBI CTAG 2 cut(s) 302, 705
GluI GCNGC 4 cut(s) 224, 269, 368, 715
HaeIII GGCC 3 cut(s) 201, 370, 492
HapII CCGG 1 cut(s) 448
Hin1II CATG 2 cut(s) 499, 625
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 1 cut(s) 388
HpaII CCGG 1 cut(s) 448
HphI GGTGA 2 cut(s) 101, 388
Hpy166II GTNNAC 3 cut(s) 92, 501, 535
Hpy188I TCNGA 2 cut(s) 348, 711
Hpy188III TCNNGA 4 cut(s) 146, 239, 448, 514
Hpy8I GTNNAC 3 cut(s) 92, 501, 535
HpyAV CCTTC 2 cut(s) 226, 624
HpyCH4III ACNGT 2 cut(s) 469, 539
HpyCH4IV ACGT 3 cut(s) 109, 587, 757
HpyCH4V TGCA 6 cut(s) 40, 83, 103, 299, 535, 629
HpyF3I CTNAG 2 cut(s) 487, 508
HpySE526I ACGT 3 cut(s) 109, 587, 757
Hsp92II CATG 2 cut(s) 499, 625
Kpn2I TCCGGA 1 cut(s) 447
Kzo9I GATC 6 cut(s) 142, 235, 289, 577, 680, 721
LmnI GCTCC 2 cut(s) 220, 364
Lsp1109I GCAGC 3 cut(s) 235, 280, 726
LweI GCATC 2 cut(s) 70, 112
MaeI CTAG 2 cut(s) 302, 705
MaeII ACGT 3 cut(s) 109, 587, 757
MaeIII GTNAC 3 cut(s) 7, 110, 179
MalI GATC 6 cut(s) 144, 237, 291, 579, 682, 723
MbiI CCGCTC 1 cut(s) 367
MboI GATC 6 cut(s) 142, 235, 289, 577, 680, 721
MboII GAAGA 5 cut(s) 164, 529, 611, 614, 707
MflI RGATCY 2 cut(s) 577, 680
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 4 cut(s) 188, 455, 734, 742
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 8 cut(s) 130, 148, 249, 327, 428, 503, 517, 579
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 3 cut(s) 54, 129, 341
MslI CAYNNNNRTG 2 cut(s) 108, 175
MspI CCGG 1 cut(s) 448
MspR9I CCNGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 1 cut(s) 362
NdeII GATC 6 cut(s) 142, 235, 289, 577, 680, 721
NlaIII CATG 2 cut(s) 499, 625
NlaIV GGNNCC 3 cut(s) 25, 559, 682
NmuCI GTSAC 2 cut(s) 110, 179
NspI RCATGY 1 cut(s) 625
PaeI GCATGC 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 74
PceI AGGCCT 1 cut(s) 492
PctI GAATGC 1 cut(s) 555
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 1 cut(s) 388
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 4 cut(s) 225, 270, 369, 716
Ppu21I YACGTR 1 cut(s) 588
PshAI GACNNNNGTC 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 25, 559, 682
PspPI GGNCC 2 cut(s) 200, 450
PsrI GAACNNNNNNTAC 2 cut(s) 366, 398
PstNI CAGNNNCTG 1 cut(s) 352
PsuI RGATCY 2 cut(s) 577, 680
RseI CAYNNNNRTG 2 cut(s) 108, 175
SaqAI TTAA 3 cut(s) 54, 129, 341
SatI GCNGC 4 cut(s) 224, 269, 368, 715
Sau3AI GATC 6 cut(s) 142, 235, 289, 577, 680, 721
Sau96I GGNCC 2 cut(s) 200, 450
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 2 cut(s) 70, 112
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 2 cut(s) 108, 175
SmlI CTYRAG 2 cut(s) 33, 227
SmoI CTYRAG 2 cut(s) 33, 227
SnaBI TACGTA 1 cut(s) 588
SpeI ACTAGT 2 cut(s) 301, 704
SphI GCATGC 1 cut(s) 625
Sse9I AATT 4 cut(s) 188, 455, 734, 742
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 2 cut(s) 367, 504
SspMI CTAG 2 cut(s) 302, 705
StuI AGGCCT 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 469, 539
TaiI ACGT 3 cut(s) 112, 590, 760
TaqI TCGA 1 cut(s) 571
TasI AATT 4 cut(s) 188, 455, 734, 742
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 3 cut(s) 54, 129, 341
Tru9I TTAA 3 cut(s) 54, 129, 341
TscAI CASTG 2 cut(s) 357, 472
TseFI GTSAC 2 cut(s) 110, 179
TseI GCWGC 3 cut(s) 223, 268, 714
Tsp45I GTSAC 2 cut(s) 110, 179
TspDTI ATGAA 4 cut(s) 165, 606, 747, 750
TspRI CASTG 2 cut(s) 357, 472
VneI GTGCAC 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 2 cut(s) 455, 734
XceI RCATGY 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 2 cut(s) 302, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.