MD04G1025800.v1.1

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003947
Physical Location & Seq
Reverse (-)
3010664 .. 3012466
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1025800.v1.1.491

Sequence Viewer

Length: 762 bp
ATGTCGGCAGCTGTGGGCGCTATTTCTGCAACCAAAGGTGCAACTTTCAATTCTCAAATGGCAGAAGAGGAGCTCGTTAACAGTGACAAGAAAAACATGGCTGCAAGTGTTGAAAAACCGAGCCAGCTGCTACCCAAAGGTGACGATGGTCATAGTGATGAGGTTGATCTGGATGAAGAGGACGATGAGGTCGCCGCATTGGAATCGGAAAAGGATCAGTTGGTTCTTGGCCCTCAGTTTTCTCTCAAGGAGCAGCTTGAGAAAGATAAAGATGATGAGAGTTTGAGGAAATGGAAGGAGCAACTTCTTGGGAGTGTTGATCTCTCTGCTGTTGGAGGTAGTAAAGAACCGGAAGTGAGGGTACTAAGTCTCACAATCGTCTGCCGGGGCCGGCCGGATCTTGTCCTGCCGATCCCGCTTACTCACAAACCCAAGAGCAGTCTGTTCACACTCAAAGAAGGATGCCAGTACCGCATCAAATTCACATTCTCCGTCTCCAAAAACATCGTCTCAGGCCTCAAGTACACCAACACTGTGTGGAAGACTGGTGTTAGAGTGGACAATTCAAAGAGAATGCTTGGAACATTTAGTCCTCAAGAAGAACCTTATATATATGAGACTGCAGAAGATACTGTTCCTGCAAGCATATTTGCCAGGGGCTGGTATTGTGTGAGAACCAAATTTTTGGATGATGATGGAAAGTGCTATTTGGACATGAGCTACTACTTTGAAATCCAGAAGAACTGGCCAAAATCCACTTGA

Protein Analysis

254

Amino Acids

28.34

Weight (kDa)

4.98

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 66 - 248 6.4e-79 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 660
AciI CCGC 3 cut(s) 195, 416, 472
AclWI GGATC 3 cut(s) 222, 405, 406
AcoI YGGCCR 2 cut(s) 392, 746
AcsI RAATTY 2 cut(s) 479, 680
AdeI CACNNNGTG 1 cut(s) 537
AfaI GTAC 3 cut(s) 363, 470, 524
AfiI CCNNNNNNNGG 1 cut(s) 660
AgsI TTSAA 4 cut(s) 49, 113, 567, 731
AjnI CCWGG 1 cut(s) 653
AloI GAACNNNNNNTCC 2 cut(s) 574, 606
AluBI AGCT 5 cut(s) 11, 73, 127, 256, 720
AluI AGCT 5 cut(s) 11, 73, 127, 256, 720
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 4 cut(s) 374, 499, 514, 611
AlwI GGATC 3 cut(s) 222, 405, 406
AlwNI CAGNNNCTG 1 cut(s) 660
AoxI GGCC 5 cut(s) 229, 388, 392, 514, 746
ApeKI GCWGC 4 cut(s) 8, 101, 127, 253
ApoI RAATTY 2 cut(s) 479, 680
ArsI GACNNNNNNTTYG 2 cut(s) 492, 524
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 2 cut(s) 230, 388
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 1 cut(s) 152
BalI TGGCCA 1 cut(s) 748
BanII GRGCYC 1 cut(s) 75
BarI GAAGNNNNNNTAC 2 cut(s) 345, 377
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 4 cut(s) 20, 88, 114, 265
BccI CCATC 2 cut(s) 140, 689
BcgI CGANNNNNNTGC 4 cut(s) 109, 143, 186, 220
BciT130I CCWGG 1 cut(s) 655
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 4 cut(s) 374, 499, 514, 611
BfmI CTRYAG 1 cut(s) 621
BfoI RGCGCY 1 cut(s) 21
BisI GCNGC 5 cut(s) 9, 102, 128, 195, 254
BlsI GCNGC 5 cut(s) 10, 103, 129, 196, 255
Bme1390I CCNGG 2 cut(s) 386, 655
BmgT120I GGNCC 2 cut(s) 230, 388
BmiI GGNNCC 1 cut(s) 389
BmrFI CCNGG 2 cut(s) 386, 655
BmsI GCATC 2 cut(s) 452, 483
BoxI GACNNNNGTC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 548
BpuEI CTTGAG 4 cut(s) 230, 278, 503, 579
BpuMI CCSGG 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 385, 654
BsaWI WCCGGW 1 cut(s) 349
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 3 cut(s) 466, 550, 749
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 385, 654
BseGI GGATG 3 cut(s) 178, 467, 694
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMII CTCAG 2 cut(s) 248, 525
BseNI ACTGG 3 cut(s) 466, 550, 749
BseRI GAGGAG 1 cut(s) 83
BseX3I CGGCCG 1 cut(s) 392
BseXI GCAGC 4 cut(s) 20, 88, 114, 265
Bsh1285I CGRYCG 1 cut(s) 395
BshFI GGCC 5 cut(s) 231, 390, 394, 516, 748
BsiEI CGRYCG 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 4 cut(s) 350, 385, 391, 395
BslI CCNNNNNNNGG 1 cut(s) 660
BsmAI GTCTC 4 cut(s) 374, 499, 514, 611
BsmBI CGTCTC 2 cut(s) 499, 514
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 5 cut(s) 231, 390, 394, 516, 748
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 5 cut(s) 166, 214, 319, 397, 411
BspACI CCGC 3 cut(s) 195, 416, 472
BspANI GGCC 5 cut(s) 231, 390, 394, 516, 748
BspCNI CTCAG 2 cut(s) 247, 524
BspLI GGNNCC 1 cut(s) 389
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 3 cut(s) 222, 405, 406
BsrFI RCCGGY 1 cut(s) 390
BsrI ACTGG 3 cut(s) 466, 550, 749
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 2 cut(s) 385, 654
BssMI GATC 5 cut(s) 166, 214, 319, 397, 411
Bst2UI CCWGG 1 cut(s) 655
Bst4CI ACNGT 3 cut(s) 83, 535, 634
Bst6I CTCTTC 2 cut(s) 60, 171
BstC8I GCNNGC 3 cut(s) 125, 392, 643
BstDEI CTNAG 3 cut(s) 234, 365, 511
BstF5I GGATG 3 cut(s) 178, 467, 694
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 5 cut(s) 169, 217, 322, 400, 414
BstMAI GTCTC 4 cut(s) 374, 499, 514, 611
BstMBI GATC 5 cut(s) 166, 214, 319, 397, 411
BstMCI CGRYCG 1 cut(s) 395
BstMWI GCNNNNNNNGC 4 cut(s) 17, 26, 415, 471
BstNI CCWGG 1 cut(s) 655
BstPAI GACNNNNGTC 1 cut(s) 147
BstSCI CCNGG 2 cut(s) 384, 653
BstSFI CTRYAG 1 cut(s) 621
BstV1I GCAGC 4 cut(s) 20, 88, 114, 265
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 397
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 1 cut(s) 397
BstZI CGGCCG 1 cut(s) 392
BsuRI GGCC 5 cut(s) 231, 390, 394, 516, 748
BtsCI GGATG 3 cut(s) 178, 467, 694
BtsIMutI CAGTG 2 cut(s) 88, 531
Cac8I GCNNGC 3 cut(s) 125, 392, 643
CaiI CAGNNNCTG 1 cut(s) 660
CfoI GCGC 1 cut(s) 20
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 2 cut(s) 230, 388
Csp6I GTAC 3 cut(s) 362, 469, 523
CviAII CATG 2 cut(s) 97, 715
CviQI GTAC 3 cut(s) 362, 469, 523
DdeI CTNAG 3 cut(s) 234, 365, 511
DpnI GATC 5 cut(s) 168, 216, 321, 399, 413
DpnII GATC 5 cut(s) 166, 214, 319, 397, 411
DraIII CACNNNGTG 1 cut(s) 537
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 2 cut(s) 392, 746
EagI CGGCCG 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 60, 171
EarI CTCTTC 2 cut(s) 60, 171
Ecl136II GAGCTC 1 cut(s) 73
EclXI CGGCCG 1 cut(s) 392
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 75
Eco52I CGGCCG 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 75
Esp3I CGTCTC 2 cut(s) 499, 514
FaeI CATG 2 cut(s) 100, 718
FaiI YATR 8 cut(s) 98, 153, 609, 611, 613, 615, 647, 716
FatI CATG 2 cut(s) 96, 714
FauI CCCGC 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 9, 102, 128, 195, 254
FokI GGATG 3 cut(s) 185, 474, 701
FriOI GRGCYC 1 cut(s) 75
FseI GGCCGGCC 1 cut(s) 394
Fsp4HI GCNGC 5 cut(s) 9, 102, 128, 195, 254
GlaI GCGC 1 cut(s) 19
GluI GCNGC 5 cut(s) 9, 102, 128, 195, 254
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 5 cut(s) 231, 390, 394, 516, 748
HapII CCGG 4 cut(s) 350, 385, 391, 395
HhaI GCGC 1 cut(s) 20
Hin1II CATG 2 cut(s) 100, 718
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 1 cut(s) 203
HpaI GTTAAC 1 cut(s) 79
HpaII CCGG 4 cut(s) 350, 385, 391, 395
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 4 cut(s) 79, 447, 525, 559
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 3 cut(s) 170, 596, 736
Hpy8I GTNNAC 4 cut(s) 79, 447, 525, 559
HpyAV CCTTC 2 cut(s) 289, 452
HpyCH4III ACNGT 3 cut(s) 83, 535, 634
HpyCH4V TGCA 5 cut(s) 29, 41, 104, 623, 641
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 26, 415, 471
HpyF3I CTNAG 3 cut(s) 234, 365, 511
Hsp92II CATG 2 cut(s) 100, 718
HspAI GCGC 1 cut(s) 18
KroI GCCGGC 1 cut(s) 390
KroNI GCCGGC 1 cut(s) 392
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 5 cut(s) 166, 214, 319, 397, 411
LmnI GCTCC 3 cut(s) 70, 250, 298
Lsp1109I GCAGC 4 cut(s) 20, 88, 114, 265
LweI GCATC 2 cut(s) 452, 483
MaeIII GTNAC 2 cut(s) 83, 140
MalI GATC 5 cut(s) 168, 216, 321, 399, 413
MboI GATC 5 cut(s) 166, 214, 319, 397, 411
MboII GAAGA 6 cut(s) 77, 188, 553, 611, 638, 751
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 1 cut(s) 75
MlsI TGGCCA 1 cut(s) 748
MluCI AATT 4 cut(s) 49, 479, 562, 680
MluNI TGGCCA 1 cut(s) 748
MmeI TCCRAC 1 cut(s) 313
Mox20I TGGCCA 1 cut(s) 748
MroNI GCCGGC 1 cut(s) 390
MscI TGGCCA 1 cut(s) 748
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 748
MspA1I CMGCKG 2 cut(s) 11, 127
MspI CCGG 4 cut(s) 350, 385, 391, 395
MspR9I CCNGG 2 cut(s) 386, 655
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 1 cut(s) 655
MwoI GCNNNNNNNGC 4 cut(s) 17, 26, 415, 471
NaeI GCCGGC 1 cut(s) 392
NciI CCSGG 1 cut(s) 386
NdeII GATC 5 cut(s) 166, 214, 319, 397, 411
NgoMIV GCCGGC 1 cut(s) 390
NlaIII CATG 2 cut(s) 100, 718
NlaIV GGNNCC 1 cut(s) 389
NmuCI GTSAC 2 cut(s) 83, 140
PceI AGGCCT 1 cut(s) 516
PctI GAATGC 1 cut(s) 579
PdiI GCCGGC 1 cut(s) 392
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 660
PkrI GCNGC 5 cut(s) 10, 103, 129, 196, 255
PshAI GACNNNNGTC 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 653
PspGI CCWGG 1 cut(s) 653
PspN4I GGNNCC 1 cut(s) 389
PspPI GGNCC 2 cut(s) 230, 388
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 1 cut(s) 660
PsuI RGATCY 1 cut(s) 397
PvuII CAGCTG 2 cut(s) 11, 127
RigI GGCCGGCC 1 cut(s) 394
RsaI GTAC 3 cut(s) 363, 470, 524
RsaNI GTAC 3 cut(s) 362, 469, 523
RseI CAYNNNNRTG 1 cut(s) 156
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 5 cut(s) 9, 102, 128, 195, 254
Sau3AI GATC 5 cut(s) 166, 214, 319, 397, 411
Sau96I GGNCC 2 cut(s) 230, 388
ScrFI CCNGG 2 cut(s) 386, 655
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 2 cut(s) 452, 483
SfcI CTRYAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 4 cut(s) 245, 257, 518, 594
SmoI CTYRAG 4 cut(s) 245, 257, 518, 594
Sse9I AATT 4 cut(s) 49, 479, 562, 680
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 3 cut(s) 195, 416, 472
SstI GAGCTC 1 cut(s) 75
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 2 cut(s) 384, 653
TaaI ACNGT 3 cut(s) 83, 535, 634
TasI AATT 4 cut(s) 49, 479, 562, 680
TatI WGTACW 1 cut(s) 522
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 2 cut(s) 88, 538
TseFI GTSAC 2 cut(s) 83, 140
TseI GCWGC 4 cut(s) 8, 101, 127, 253
Tsp45I GTSAC 2 cut(s) 83, 140
TspDTI ATGAA 1 cut(s) 189
TspGWI ACGGA 1 cut(s) 481
TspRI CASTG 2 cut(s) 88, 538
Van91I CCANNNNNTGG 1 cut(s) 660
XapI RAATTY 2 cut(s) 479, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.