Rw4G013820

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003947
Physical Location & Seq
Forward (+)
32345562 .. 32346714
1153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013820.1

Sequence Viewer

Length: 726 bp
ATGTCGGCTGCCGTGGAAGCTATGTCTGCAACCAAAGATGTTGGTTTCAGTTCTCAAGTGCAGGAAGTGGAGTTTAAGAATGATAAGAACAGCAACAAGGATGCAGGTGGCGAACCAAGCCTGCATCACCATGACGAAAATGATGAGCTTAAAGATGAGGTTGATCCAGATGAAGAGGATGAGCTTGCCACATTCAAATGTGACAAAGAATTGGTTCTTGGCCCCAAGTTTTCTCTCAAGGAGCAGCTTGAGAAGGATAAAGATGACGAGAGTTTGAGGAAATGGAAGCAGCAACTTCTTGGGAGTGTTGATCTCTCTGCACTAGCTAGTAAAGAAGCAGATGTGAGGATTTTAAGTCTGACAATGATGTTCCGGGAGCGGCCTGAACTTGTGCTGCCGATTCCATTCACCAACAAACCAAAGAGCAGTCTTTTCACACTCAAAGGAGGAAGCAAGTACCGAACCAAATTCACATTCACTGTCTCCAAAAACATTGTCTCAGGCCTCATGTACACCACCTCAGTCTGGAAGACTGGTGTCAAAGTGCACAGTTCAAAAAGAATGCTTGGAACCTTTAGTCCTCGACCAGATCCTTATACGTATGAAACCGAAGAGATACTACCCCTTCAGGCATGTTTGCAAGGGTTTCTGGATGATGATGGAAAATGCTATTTGGATGCTAGTTACTACTTTGAAATCCAGAAGAACTGGGCAGCACCCTCTTGA

Protein Analysis

241

Amino Acids

27.24

Weight (kDa)

5.1

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 60 - 236 5.4e-56 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 379
AciI CCGC 1 cut(s) 379
AclWI GGATC 2 cut(s) 158, 584
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 1 cut(s) 611
AfaI GTAC 2 cut(s) 458, 512
AfiI CCNNNNNNNGG 1 cut(s) 525
AgsI TTSAA 3 cut(s) 196, 555, 695
AjuI GAANNNNNNNTTGG 4 cut(s) 201, 233, 458, 490
AloI GAACNNNNNNTCC 2 cut(s) 562, 594
AluBI AGCT 5 cut(s) 20, 148, 184, 247, 326
AluI AGCT 5 cut(s) 20, 148, 184, 247, 326
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 487, 502
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 2 cut(s) 158, 584
AoxI GGCC 3 cut(s) 220, 380, 502
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 5 cut(s) 8, 244, 289, 394, 713
ApoI RAATTY 1 cut(s) 467
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
Asp700I GAANNNNTTC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 119, 400
BaeGI GKGCMC 1 cut(s) 549
BarI GAAGNNNNNNTAC 2 cut(s) 603, 635
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 256, 301, 381
BccI CCATC 1 cut(s) 653
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 487, 502
BfaI CTAG 3 cut(s) 323, 327, 681
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 6 cut(s) 9, 245, 290, 380, 395, 714
BlsI GCNGC 6 cut(s) 10, 246, 291, 381, 396, 715
Bme1390I CCNGG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 223, 571
BmrFI CCNGG 1 cut(s) 374
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 3 cut(s) 91, 133, 667
BmuI ACTGGG 1 cut(s) 718
BoxI GACNNNNGTC 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 536
BpuEI CTTGAG 3 cut(s) 39, 221, 269
BpuMI CCSGG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 600
BsaJI CCNNGG 1 cut(s) 12
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse1I ACTGG 2 cut(s) 538, 713
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 4 cut(s) 106, 184, 658, 682
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMII CTCAG 2 cut(s) 513, 534
BseNI ACTGG 2 cut(s) 538, 713
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 3 cut(s) 256, 301, 381
BsgI GTGCAG 2 cut(s) 80, 303
BshFI GGCC 3 cut(s) 222, 382, 504
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 1 cut(s) 373
BslI CCNNNNNNNGG 1 cut(s) 525
BsmAI GTCTC 2 cut(s) 487, 502
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 3 cut(s) 222, 382, 504
Bsp1286I GDGCHC 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 163, 310, 589
BspACI CCGC 1 cut(s) 379
BspANI GGCC 3 cut(s) 222, 382, 504
BspCNI CTCAG 2 cut(s) 512, 533
BspLI GGNNCC 2 cut(s) 223, 571
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 2 cut(s) 158, 584
BsrBI CCGCTC 1 cut(s) 379
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 2 cut(s) 538, 713
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 3 cut(s) 163, 310, 589
Bst4CI ACNGT 2 cut(s) 481, 551
Bst6I CTCTTC 2 cut(s) 168, 606
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 600
BstC8I GCNNGC 2 cut(s) 122, 186
BstDEI CTNAG 2 cut(s) 499, 520
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 4 cut(s) 106, 184, 658, 682
BstKTI GATC 3 cut(s) 166, 313, 592
BstMAI GTCTC 2 cut(s) 487, 502
BstMBI GATC 3 cut(s) 163, 310, 589
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 117
BstNSI RCATGY 1 cut(s) 636
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 549
BstSNI TACGTA 1 cut(s) 600
BstV1I GCAGC 3 cut(s) 256, 301, 381
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BsuRI GGCC 3 cut(s) 222, 382, 504
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 4 cut(s) 106, 184, 658, 682
BtsIMutI CAGTG 1 cut(s) 477
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 122, 186
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 457, 511
CviAII CATG 3 cut(s) 131, 508, 633
CviQI GTAC 2 cut(s) 457, 511
DdeI CTNAG 2 cut(s) 499, 520
DpnI GATC 3 cut(s) 165, 312, 591
DpnII GATC 3 cut(s) 163, 310, 589
Eam1104I CTCTTC 2 cut(s) 168, 606
EarI CTCTTC 2 cut(s) 168, 606
Eco105I TACGTA 1 cut(s) 600
Eco147I AGGCCT 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 611
FaeI CATG 3 cut(s) 134, 511, 636
FaiI YATR 6 cut(s) 23, 132, 509, 597, 603, 634
FatI CATG 3 cut(s) 130, 507, 632
Fnu4HI GCNGC 6 cut(s) 9, 245, 290, 380, 395, 714
FokI GGATG 4 cut(s) 113, 191, 665, 689
Fsp4HI GCNGC 6 cut(s) 9, 245, 290, 380, 395, 714
FspBI CTAG 3 cut(s) 323, 327, 681
GluI GCNGC 6 cut(s) 9, 245, 290, 380, 395, 714
HaeIII GGCC 3 cut(s) 222, 382, 504
HapII CCGG 1 cut(s) 373
Hin1II CATG 3 cut(s) 134, 511, 636
HinfI GANTC 1 cut(s) 400
HpaII CCGG 1 cut(s) 373
HphI GGTGA 2 cut(s) 119, 400
Hpy166II GTNNAC 2 cut(s) 513, 547
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 5 cut(s) 167, 526, 650, 700, 723
Hpy8I GTNNAC 2 cut(s) 513, 547
HpyAV CCTTC 2 cut(s) 247, 635
HpyCH4III ACNGT 2 cut(s) 481, 551
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 7 cut(s) 29, 61, 104, 124, 320, 547, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 117
HpyF3I CTNAG 2 cut(s) 499, 520
HpySE526I ACGT 1 cut(s) 599
Hsp92II CATG 3 cut(s) 134, 511, 636
Kzo9I GATC 3 cut(s) 163, 310, 589
LmnI GCTCC 2 cut(s) 241, 376
Lsp1109I GCAGC 3 cut(s) 256, 301, 381
LweI GCATC 3 cut(s) 91, 133, 667
MaeI CTAG 3 cut(s) 323, 327, 681
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 2 cut(s) 200, 683
MalI GATC 3 cut(s) 165, 312, 591
MbiI CCGCTC 1 cut(s) 379
MboI GATC 3 cut(s) 163, 310, 589
MboII GAAGA 4 cut(s) 185, 541, 623, 715
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 2 cut(s) 209, 467
MnlI CCTC 8 cut(s) 151, 169, 270, 339, 440, 515, 529, 591
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 3 cut(s) 75, 150, 353
MslI CAYNNNNRTG 2 cut(s) 129, 196
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 567
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 117
NciI CCSGG 1 cut(s) 374
NdeII GATC 3 cut(s) 163, 310, 589
NlaIII CATG 3 cut(s) 134, 511, 636
NlaIV GGNNCC 2 cut(s) 223, 571
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 636
PaqCI CACCTGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 504
PctI GAATGC 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 6 cut(s) 10, 246, 291, 381, 396, 715
Ppu21I YACGTR 1 cut(s) 600
PshAI GACNNNNGTC 1 cut(s) 536
PspN4I GGNNCC 2 cut(s) 223, 571
PspPI GGNCC 1 cut(s) 221
PsuI RGATCY 1 cut(s) 589
RsaI GTAC 2 cut(s) 458, 512
RsaNI GTAC 2 cut(s) 457, 511
RseI CAYNNNNRTG 2 cut(s) 129, 196
SaqAI TTAA 3 cut(s) 75, 150, 353
SatI GCNGC 6 cut(s) 9, 245, 290, 380, 395, 714
Sau3AI GATC 3 cut(s) 163, 310, 589
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 374
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 3 cut(s) 91, 133, 667
SmiMI CAYNNNNRTG 2 cut(s) 129, 196
SmlI CTYRAG 3 cut(s) 54, 236, 248
SmoI CTYRAG 3 cut(s) 54, 236, 248
SnaBI TACGTA 1 cut(s) 600
Sse9I AATT 2 cut(s) 209, 467
SseBI AGGCCT 1 cut(s) 504
SsiI CCGC 1 cut(s) 379
SspMI CTAG 3 cut(s) 323, 327, 681
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 481, 551
TaiI ACGT 1 cut(s) 602
TaqI TCGA 1 cut(s) 583
TasI AATT 2 cut(s) 209, 467
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 382
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 3 cut(s) 75, 150, 353
Tru9I TTAA 3 cut(s) 75, 150, 353
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 5 cut(s) 8, 244, 289, 394, 713
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 186, 618
TspRI CASTG 1 cut(s) 484
VneI GTGCAC 1 cut(s) 545
XapI RAATTY 1 cut(s) 467
XceI RCATGY 1 cut(s) 636
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 3 cut(s) 323, 327, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.