Rh4BG164300

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003947
Physical Location & Seq
Forward (+)
29672634 .. 29677349
4716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG164300.1

Sequence Viewer

Length: 642 bp
ATGTCAGCTGCCGTGGAAGCTATGTCTGCAACCAAAGATGTTGGTTTCAGTTCTCAAGTGCAGGAAGTGGAGTTTAAGAATGATAAGAATAGCAACAAGGATGCAGGTGGCGAACCAAGCCTGCATCACCATGACGAAAATGATGAGCTTAAAGATGAGGTTGATCCAGATGAAGAGGATGAGCTTGCCACATTCAAATGTGACAAAGAATTGGTTCTTGGCCCCAAGTTTTCTCTCAAGGAGCAGCTTGAGAAGGATAAAGATGATGAGAGTTTGAGGAAATGGAAGCAGCAACTTCTTGGGAGTGTTGATCTCTCTGCACTAGCTGTTGGAGAGAGTAAAGAAGCAGAAGTGAGGATTTTAAGTCTGACAATGATGTTCCGGGAGCGGCCTGAACTTGTGCTGCCGATTCCATTCACCAACAAACCAAAGAGCACTCTTTTCACACTCAAAGGAGGAAGCAAGTACCGAACCAAATTCACATTCACTGTCTCCAAAAACATTGTCTCAGGCCTCATGTACACCACCTCAGTCTGGAAGACTGGTGTCAAAGAGAGAGATTATGATGCTAATCTTAAGGAGGGGGTCTGGTTGGTTCAAGCAGGAAAGCTAATATTACTAATTATTCTTGGTGACCGCTGA

Protein Analysis

213

Amino Acids

23.96

Weight (kDa)

5.19

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 60 - 184 3.2e-40 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 388
AciI CCGC 2 cut(s) 388, 637
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 2 cut(s) 467, 521
AfiI CCNNNNNNNGG 1 cut(s) 534
AflII CTTAAG 1 cut(s) 575
AgsI TTSAA 2 cut(s) 196, 599
AjuI GAANNNNNNNTTGG 4 cut(s) 201, 233, 467, 499
AluBI AGCT 7 cut(s) 8, 20, 148, 184, 247, 326, 610
AluI AGCT 7 cut(s) 8, 20, 148, 184, 247, 326, 610
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 2 cut(s) 496, 511
AlwI GGATC 1 cut(s) 158
AoxI GGCC 3 cut(s) 220, 389, 511
ApeKI GCWGC 4 cut(s) 8, 244, 289, 403
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
Asp700I GAANNNNTTC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 119, 409
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 256, 301, 390
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 2 cut(s) 496, 511
BfaI CTAG 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 5 cut(s) 9, 245, 290, 389, 404
BlsI GCNGC 5 cut(s) 10, 246, 291, 390, 405
Bme1390I CCNGG 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 383
BmsI GCATC 3 cut(s) 91, 133, 556
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 545
BpuEI CTTGAG 3 cut(s) 39, 221, 269
BpuMI CCSGG 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 12
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse1I ACTGG 1 cut(s) 547
Bse8I GATNNNNATC 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 2 cut(s) 106, 184
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 522, 543
BseNI ACTGG 1 cut(s) 547
BseXI GCAGC 3 cut(s) 256, 301, 390
BsgI GTGCAG 2 cut(s) 80, 303
BshFI GGCC 3 cut(s) 222, 391, 513
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 382
BslI CCNNNNNNNGG 1 cut(s) 534
BsmAI GTCTC 2 cut(s) 496, 511
BsnI GGCC 3 cut(s) 222, 391, 513
Bsp1286I GDGCHC 1 cut(s) 437
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 2 cut(s) 163, 310
BspACI CCGC 2 cut(s) 388, 637
BspANI GGCC 3 cut(s) 222, 391, 513
BspCNI CTCAG 2 cut(s) 521, 542
BspLI GGNNCC 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 575
BsrBI CCGCTC 1 cut(s) 388
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 2 cut(s) 163, 310
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 575
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 122, 186
BstDEI CTNAG 2 cut(s) 508, 529
BstDSI CCRYGG 1 cut(s) 12
BstEII GGTNACC 1 cut(s) 632
BstF5I GGATG 2 cut(s) 106, 184
BstKTI GATC 2 cut(s) 166, 313
BstMAI GTCTC 2 cut(s) 496, 511
BstMBI GATC 2 cut(s) 163, 310
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 117
BstPAI GACNNNNGTC 1 cut(s) 545
BstPI GGTNACC 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 256, 301, 390
BstV2I GAAGAC 1 cut(s) 545
BsuRI GGCC 3 cut(s) 222, 391, 513
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 106, 184
BtsIMutI CAGTG 1 cut(s) 486
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 122, 186
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 466, 520
CviAII CATG 2 cut(s) 131, 517
CviQI GTAC 2 cut(s) 466, 520
DdeI CTNAG 2 cut(s) 508, 529
DpnI GATC 2 cut(s) 165, 312
DpnII GATC 2 cut(s) 163, 310
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco147I AGGCCT 1 cut(s) 513
Eco91I GGTNACC 1 cut(s) 632
EcoO65I GGTNACC 1 cut(s) 632
FaeI CATG 2 cut(s) 134, 520
FaiI YATR 4 cut(s) 23, 132, 518, 564
FatI CATG 2 cut(s) 130, 516
Fnu4HI GCNGC 5 cut(s) 9, 245, 290, 389, 404
FokI GGATG 2 cut(s) 113, 191
Fsp4HI GCNGC 5 cut(s) 9, 245, 290, 389, 404
FspBI CTAG 1 cut(s) 323
GluI GCNGC 5 cut(s) 9, 245, 290, 389, 404
HaeIII GGCC 3 cut(s) 222, 391, 513
HapII CCGG 1 cut(s) 382
Hin1II CATG 2 cut(s) 134, 520
HinfI GANTC 1 cut(s) 409
HpaII CCGG 1 cut(s) 382
HphI GGTGA 2 cut(s) 119, 409
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 2 cut(s) 167, 535
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 5 cut(s) 29, 61, 104, 124, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 117
HpyF3I CTNAG 2 cut(s) 508, 529
Hsp92II CATG 2 cut(s) 134, 520
Kzo9I GATC 2 cut(s) 163, 310
LmnI GCTCC 2 cut(s) 241, 385
Lsp1109I GCAGC 3 cut(s) 256, 301, 390
LweI GCATC 3 cut(s) 91, 133, 556
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 200, 632
MalI GATC 2 cut(s) 165, 312
MbiI CCGCTC 1 cut(s) 388
MboI GATC 2 cut(s) 163, 310
MboII GAAGA 2 cut(s) 185, 550
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 3 cut(s) 209, 476, 621
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 8 cut(s) 151, 169, 270, 348, 449, 524, 538, 574
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 4 cut(s) 75, 150, 362, 576
MslI CAYNNNNRTG 2 cut(s) 129, 196
MspA1I CMGCKG 2 cut(s) 8, 639
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 1 cut(s) 382
MspR9I CCNGG 1 cut(s) 383
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 117
NciI CCSGG 1 cut(s) 383
NdeII GATC 2 cut(s) 163, 310
NlaIII CATG 2 cut(s) 134, 520
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 200, 632
PaqCI CACCTGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 5 cut(s) 10, 246, 291, 390, 405
PshAI GACNNNNGTC 1 cut(s) 545
PspEI GGTNACC 1 cut(s) 632
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 467, 521
RsaNI GTAC 2 cut(s) 466, 520
RseI CAYNNNNRTG 2 cut(s) 129, 196
SaqAI TTAA 4 cut(s) 75, 150, 362, 576
SatI GCNGC 5 cut(s) 9, 245, 290, 389, 404
Sau3AI GATC 2 cut(s) 163, 310
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 1 cut(s) 437
SfaNI GCATC 3 cut(s) 91, 133, 556
SmiMI CAYNNNNRTG 2 cut(s) 129, 196
SmlI CTYRAG 4 cut(s) 54, 236, 248, 575
SmoI CTYRAG 4 cut(s) 54, 236, 248, 575
Sse9I AATT 3 cut(s) 209, 476, 621
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 2 cut(s) 388, 637
SspI AATATT 1 cut(s) 615
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 490
TasI AATT 3 cut(s) 209, 476, 621
TatI WGTACW 1 cut(s) 519
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 4 cut(s) 75, 150, 362, 576
Tru9I TTAA 4 cut(s) 75, 150, 362, 576
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 2 cut(s) 200, 632
TseI GCWGC 4 cut(s) 8, 244, 289, 403
Tsp45I GTSAC 2 cut(s) 200, 632
TspDTI ATGAA 1 cut(s) 186
TspRI CASTG 1 cut(s) 493
Vha464I CTTAAG 1 cut(s) 575
XapI RAATTY 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.