Rw4G013900

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003947
Physical Location & Seq
Forward (+)
32480160 .. 32481261
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013900.1

Sequence Viewer

Length: 714 bp
ATGTCTATAACCAAAGATGTTGGTTCCAGTTCTCAAGTGCAGGAAGTGGAGTTTAAGAATGATAAGAATAGCAACAAGGATGCAGGTGGTGAACCAAGCCTACATCAACGTGACGAAAATGATAAGCTTAAAGATGAGGTTGATCCAGATGAAGAGGATGAGCTTGCCACATTCAAATGTGACAAAGAATTGGTTCTTGGCCCTAAGTTTTCTCTCAAGGAGCAGCTTGAGAAGGATAAAGATGATGAAAGTTTGAGGAAATGGAAGCAGCAACTTCTTGGGAGAGTTGATCTCTCTGCACTAGCTAGTAAAGAATCAGAAGTGAGGATTTTAAGTATGACACTGATGTTCCAGGAGCGGCCTGAACTTGTGCTGCCGATTCCATTCACCAACAAACCAAAGAGCAATCTTTTCACACTCAAAGGAGGAAGCAAGTTCCGGACCAAATTCACATTTACTGTCTCAAAAAACATTGTCTCAGGCCTCAAGTTCACCGGCTCAATCTGGAAGACTGGTGTCAAAGTGCACAGTTCAAAAAGAATGCTTGGAACCTTTAGTCCTCGACCAGATCCTTATACGTATGAAACCGAAGAAGATACTACCCCTTCAGGCATGCTTGCAAGGGGCAAGTATTTTGTCAGAATAAAATTTCTGGATGATGATGGAAAATGCTATTTGGATGCTAGTTACTACTTTGAAATCCAGAAGAACTAG

Protein Analysis

237

Amino Acids

27.08

Weight (kDa)

6.24

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 53 - 237 1.8e-71 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 74
AccBSI CCGCTC 1 cut(s) 358
AccIII TCCGGA 1 cut(s) 438
AciI CCGC 1 cut(s) 358
AclWI GGATC 2 cut(s) 137, 563
AcsI RAATTY 2 cut(s) 446, 647
AcuI CTGAAG 1 cut(s) 591
AgsI TTSAA 3 cut(s) 175, 534, 698
AjnI CCWGG 1 cut(s) 351
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AloI GAACNNNNNNTCC 2 cut(s) 541, 573
AluBI AGCT 4 cut(s) 127, 163, 226, 305
AluI AGCT 4 cut(s) 127, 163, 226, 305
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 2 cut(s) 466, 481
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 2 cut(s) 137, 563
Aor13HI TCCGGA 1 cut(s) 438
AoxI GGCC 3 cut(s) 199, 359, 481
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 3 cut(s) 223, 268, 373
ApoI RAATTY 2 cut(s) 446, 647
Asp700I GAANNNNTTC 1 cut(s) 192
AspS9I GGNCC 2 cut(s) 200, 441
AsuHPI GGTGA 3 cut(s) 101, 379, 484
AvaII GGWCC 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 235, 280, 360
BccI CCATC 1 cut(s) 656
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 466, 481
BfaI CTAG 4 cut(s) 302, 306, 684, 712
BfuAI ACCTGC 1 cut(s) 74
BisI GCNGC 4 cut(s) 224, 269, 359, 374
BlsI GCNGC 4 cut(s) 225, 270, 360, 375
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 441
BmgT120I GGNCC 2 cut(s) 200, 441
BmiI GGNNCC 2 cut(s) 25, 550
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 2 cut(s) 70, 670
BoxI GACNNNNGTC 1 cut(s) 515
BpiI GAAGAC 1 cut(s) 515
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpuEI CTTGAG 4 cut(s) 18, 200, 248, 470
BsaAI YACGTR 1 cut(s) 579
BsaWI WCCGGW 1 cut(s) 438
Bse118I RCCGGY 1 cut(s) 494
Bse1I ACTGG 2 cut(s) 27, 517
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 1 cut(s) 353
BseGI GGATG 4 cut(s) 85, 163, 661, 685
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 2 cut(s) 27, 517
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 3 cut(s) 235, 280, 360
BsgI GTGCAG 2 cut(s) 59, 282
BshFI GGCC 3 cut(s) 201, 361, 483
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 2 cut(s) 439, 495
BsmAI GTCTC 2 cut(s) 466, 481
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 3 cut(s) 201, 361, 483
Bsp1286I GDGCHC 1 cut(s) 528
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 3 cut(s) 142, 289, 568
BspACI CCGC 1 cut(s) 358
BspANI GGCC 3 cut(s) 201, 361, 483
BspCNI CTCAG 1 cut(s) 491
BspEI TCCGGA 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 25, 550
BspMI ACCTGC 1 cut(s) 74
BspPI GGATC 2 cut(s) 137, 563
BsrBI CCGCTC 1 cut(s) 358
BsrFI RCCGGY 1 cut(s) 494
BsrI ACTGG 2 cut(s) 27, 517
BssAI RCCGGY 1 cut(s) 494
BssMI GATC 3 cut(s) 142, 289, 568
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 2 cut(s) 460, 530
Bst6I CTCTTC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 579
BstC8I GCNNGC 3 cut(s) 165, 614, 618
BstDEI CTNAG 2 cut(s) 204, 478
BstF5I GGATG 4 cut(s) 85, 163, 661, 685
BstKTI GATC 3 cut(s) 145, 292, 571
BstMAI GTCTC 2 cut(s) 466, 481
BstMBI GATC 3 cut(s) 142, 289, 568
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 616
BstPAI GACNNNNGTC 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 528
BstSNI TACGTA 1 cut(s) 579
BstV1I GCAGC 3 cut(s) 235, 280, 360
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 3 cut(s) 201, 361, 483
BtsCI GGATG 4 cut(s) 85, 163, 661, 685
BtsIMutI CAGTG 1 cut(s) 341
BveI ACCTGC 1 cut(s) 74
Cac8I GCNNGC 3 cut(s) 165, 614, 618
Cfr10I RCCGGY 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 200, 441
CviAII CATG 1 cut(s) 613
CviJI RGCY 9 cut(s) 99, 127, 163, 201, 226, 305, 361, 483, 498
CviKI_1 RGCY 9 cut(s) 99, 127, 163, 201, 226, 305, 361, 483, 498
DdeI CTNAG 2 cut(s) 204, 478
DpnI GATC 3 cut(s) 144, 291, 570
DpnII GATC 3 cut(s) 142, 289, 568
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco105I TACGTA 1 cut(s) 579
Eco147I AGGCCT 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 351
FaeI CATG 1 cut(s) 616
FaiI YATR 5 cut(s) 8, 338, 576, 582, 614
FatI CATG 1 cut(s) 612
Fnu4HI GCNGC 4 cut(s) 224, 269, 359, 374
FokI GGATG 4 cut(s) 92, 170, 668, 692
Fsp4HI GCNGC 4 cut(s) 224, 269, 359, 374
FspBI CTAG 4 cut(s) 302, 306, 684, 712
GluI GCNGC 4 cut(s) 224, 269, 359, 374
HaeIII GGCC 3 cut(s) 201, 361, 483
HapII CCGG 2 cut(s) 439, 495
Hin1II CATG 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 2 cut(s) 314, 379
HpaII CCGG 2 cut(s) 439, 495
HphI GGTGA 3 cut(s) 101, 379, 484
Hpy166II GTNNAC 3 cut(s) 92, 492, 526
Hpy188I TCNGA 2 cut(s) 319, 641
Hpy188III TCNNGA 5 cut(s) 146, 439, 505, 653, 703
Hpy8I GTNNAC 3 cut(s) 92, 492, 526
HpyAV CCTTC 2 cut(s) 226, 615
HpyCH4III ACNGT 2 cut(s) 460, 530
HpyCH4IV ACGT 2 cut(s) 109, 578
HpyCH4V TGCA 5 cut(s) 40, 83, 299, 526, 620
HpyF3I CTNAG 2 cut(s) 204, 478
HpySE526I ACGT 2 cut(s) 109, 578
Hsp92II CATG 1 cut(s) 616
Kpn2I TCCGGA 1 cut(s) 438
Kzo9I GATC 3 cut(s) 142, 289, 568
LmnI GCTCC 2 cut(s) 220, 355
Lsp1109I GCAGC 3 cut(s) 235, 280, 360
LweI GCATC 2 cut(s) 70, 670
MaeI CTAG 4 cut(s) 302, 306, 684, 712
MaeII ACGT 2 cut(s) 109, 578
MaeIII GTNAC 3 cut(s) 110, 179, 686
MalI GATC 3 cut(s) 144, 291, 570
MbiI CCGCTC 1 cut(s) 358
MboI GATC 3 cut(s) 142, 289, 568
MboII GAAGA 4 cut(s) 164, 520, 602, 605
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 3 cut(s) 188, 446, 647
MnlI CCTC 7 cut(s) 130, 148, 249, 318, 419, 494, 570
MroI TCCGGA 1 cut(s) 438
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 3 cut(s) 54, 129, 332
MslI CAYNNNNRTG 2 cut(s) 108, 175
MspI CCGG 2 cut(s) 439, 495
MspR9I CCNGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 546
MvaI CCWGG 1 cut(s) 353
NdeII GATC 3 cut(s) 142, 289, 568
NlaIII CATG 1 cut(s) 616
NlaIV GGNNCC 2 cut(s) 25, 550
NmuCI GTSAC 2 cut(s) 110, 179
NspI RCATGY 1 cut(s) 616
PaeI GCATGC 1 cut(s) 616
PaqCI CACCTGC 1 cut(s) 74
PceI AGGCCT 1 cut(s) 483
PctI GAATGC 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 2 cut(s) 314, 379
PfoI TCCNGGA 1 cut(s) 351
PkrI GCNGC 4 cut(s) 225, 270, 360, 375
Ppu21I YACGTR 1 cut(s) 579
PshAI GACNNNNGTC 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 25, 550
PspPI GGNCC 2 cut(s) 200, 441
PsrI GAACNNNNNNTAC 2 cut(s) 84, 116
PsuI RGATCY 1 cut(s) 568
RseI CAYNNNNRTG 2 cut(s) 108, 175
SaqAI TTAA 3 cut(s) 54, 129, 332
SatI GCNGC 4 cut(s) 224, 269, 359, 374
Sau3AI GATC 3 cut(s) 142, 289, 568
Sau96I GGNCC 2 cut(s) 200, 441
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 528
SetI ASST 9 cut(s) 88, 112, 129, 141, 165, 228, 307, 554, 581
SfaNI GCATC 2 cut(s) 70, 670
SinI GGWCC 1 cut(s) 441
SmiMI CAYNNNNRTG 2 cut(s) 108, 175
SmlI CTYRAG 4 cut(s) 33, 215, 227, 485
SmoI CTYRAG 4 cut(s) 33, 215, 227, 485
SnaBI TACGTA 1 cut(s) 579
SphI GCATGC 1 cut(s) 616
Sse9I AATT 3 cut(s) 188, 446, 647
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 1 cut(s) 358
SspMI CTAG 4 cut(s) 302, 306, 684, 712
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 460, 530
TaiI ACGT 2 cut(s) 112, 581
TaqI TCGA 1 cut(s) 562
TasI AATT 3 cut(s) 188, 446, 647
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 2 cut(s) 314, 379
Tru1I TTAA 3 cut(s) 54, 129, 332
Tru9I TTAA 3 cut(s) 54, 129, 332
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 2 cut(s) 110, 179
TseI GCWGC 3 cut(s) 223, 268, 373
Tsp45I GTSAC 2 cut(s) 110, 179
TspDTI ATGAA 3 cut(s) 165, 261, 597
TspRI CASTG 1 cut(s) 348
VneI GTGCAC 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 2 cut(s) 446, 647
XceI RCATGY 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 4 cut(s) 302, 306, 684, 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.