Rroxscaffold_5G00355930

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003947
Physical Location & Seq
Forward (+)
35083184 .. 35084689
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355930.1

Sequence Viewer

Length: 759 bp
ATGTCGGCTGCCGTGGAAGCTATGTCTGCAACCAAAGATGTCGGCTTCAGTTCTCCAGTGCAGGAAGTGGAGTTTAAGAATGATAAGAACAGCAACAAGGATGCAGGTGGCGAACCAAGCCTGCATCACCATGACGAAAATGATGAGCTTAAAGATGAGGTTGATCCAGATGAAGCAGATGAGCTTGCCACATTCAAATGTGACAAAGAAATGGTTCTTGGCCCCAAGTTTTCTCTCAAGGAGCAGCTTGAGAAGGATAAAGATGATGAGAGTTTGAGGAAATGGAAGCAGCAACTTCTTGGGAGTGTTGATCTCTCTGCACTAGCTGTTGGAGAGAGTAAAGAAGCAGAAGTGAGGATTTTAAGTCTGACAATGATGTTCCGGGAGCGGCCTGAACTTGTACTGCCGATTCCATTCACCAACAAACCAAAGAGCAGTCTTTTCACACTCAAAGGAGGAAGCAAGTACCGGACCAAATTTACATTCACTGTCTCCAAAAACATTGTCTCAGGCCTCATGTACACCACCTCAGTCTGGAAGACTGGTGTCAAAGTGCACAGTTCAAAAAGAATGCTTGGAACATTTAGTCCTCGACCAGATCCTTATACACATGAAACCGAAGAAGATACTACCCCTTCAGGCATGTTTGCAAGGGGCTGGTATTTTGTGAGAACAAAATTTCTGGATGATGATGGAAAATGCTATTTGGATGCTAGTTACTACTTTGAAATCCAGAAGAACTGGGCAGCACCCTCTTGA

Protein Analysis

252

Amino Acids

28.51

Weight (kDa)

5.4

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 63 - 247 6.2e-74 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 388
AciI CCGC 1 cut(s) 388
AclWI GGATC 2 cut(s) 158, 593
AcsI RAATTY 2 cut(s) 476, 677
AcuI CTGAAG 2 cut(s) 31, 621
AfaI GTAC 3 cut(s) 402, 467, 521
AfiI CCNNNNNNNGG 1 cut(s) 534
AgsI TTSAA 3 cut(s) 196, 564, 728
AjuI GAANNNNNNNTTGG 4 cut(s) 201, 233, 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 571, 603
AluBI AGCT 5 cut(s) 20, 148, 184, 247, 326
AluI AGCT 5 cut(s) 20, 148, 184, 247, 326
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 2 cut(s) 496, 511
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 2 cut(s) 158, 593
AoxI GGCC 3 cut(s) 220, 389, 511
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 4 cut(s) 8, 244, 289, 746
ApoI RAATTY 2 cut(s) 476, 677
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
Asp700I GAANNNNTTC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 221, 471
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 119, 409
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 256, 301
BccI CCATC 1 cut(s) 686
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 2 cut(s) 496, 511
BfaI CTAG 2 cut(s) 323, 714
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 5 cut(s) 9, 245, 290, 389, 747
BlsI GCNGC 5 cut(s) 10, 246, 291, 390, 748
Bme1390I CCNGG 1 cut(s) 383
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 221, 471
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 383
BmrI ACTGGG 1 cut(s) 751
BmsI GCATC 3 cut(s) 91, 133, 700
BmuI ACTGGG 1 cut(s) 751
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 545
BpmI CTGGAG 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 221, 269
BpuMI CCSGG 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 468
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse1I ACTGG 3 cut(s) 56, 547, 746
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 106, 691, 715
BseLI CCNNNNNNNGG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 522, 543
BseNI ACTGG 3 cut(s) 56, 547, 746
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 2 cut(s) 256, 301
BsgI GTGCAG 2 cut(s) 80, 303
BshFI GGCC 3 cut(s) 222, 391, 513
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 2 cut(s) 382, 469
BslI CCNNNNNNNGG 1 cut(s) 534
BsmAI GTCTC 2 cut(s) 496, 511
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 3 cut(s) 222, 391, 513
Bsp1286I GDGCHC 1 cut(s) 558
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 3 cut(s) 163, 310, 598
BspACI CCGC 1 cut(s) 388
BspANI GGCC 3 cut(s) 222, 391, 513
BspCNI CTCAG 2 cut(s) 521, 542
BspLI GGNNCC 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 2 cut(s) 158, 593
BsrBI CCGCTC 1 cut(s) 388
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 3 cut(s) 56, 547, 746
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 3 cut(s) 163, 310, 598
Bst4CI ACNGT 2 cut(s) 490, 560
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 122, 186
BstDEI CTNAG 2 cut(s) 508, 529
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 106, 691, 715
BstKTI GATC 3 cut(s) 166, 313, 601
BstMAI GTCTC 2 cut(s) 496, 511
BstMBI GATC 3 cut(s) 163, 310, 598
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 117
BstNSI RCATGY 1 cut(s) 646
BstPAI GACNNNNGTC 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 256, 301
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 3 cut(s) 222, 391, 513
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 3 cut(s) 106, 691, 715
BtsIMutI CAGTG 2 cut(s) 63, 486
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 122, 186
Cfr13I GGNCC 2 cut(s) 221, 471
Csp6I GTAC 3 cut(s) 401, 466, 520
CviAII CATG 4 cut(s) 131, 517, 611, 643
CviQI GTAC 3 cut(s) 401, 466, 520
DdeI CTNAG 2 cut(s) 508, 529
DpnI GATC 3 cut(s) 165, 312, 600
DpnII GATC 3 cut(s) 163, 310, 598
Eco147I AGGCCT 1 cut(s) 513
Eco47I GGWCC 1 cut(s) 471
Eco57I CTGAAG 2 cut(s) 31, 621
FaeI CATG 4 cut(s) 134, 520, 614, 646
FaiI YATR 6 cut(s) 23, 132, 518, 606, 612, 644
FatI CATG 4 cut(s) 130, 516, 610, 642
Fnu4HI GCNGC 5 cut(s) 9, 245, 290, 389, 747
FokI GGATG 3 cut(s) 113, 698, 722
Fsp4HI GCNGC 5 cut(s) 9, 245, 290, 389, 747
FspBI CTAG 2 cut(s) 323, 714
GluI GCNGC 5 cut(s) 9, 245, 290, 389, 747
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 3 cut(s) 222, 391, 513
HapII CCGG 2 cut(s) 382, 469
Hin1II CATG 4 cut(s) 134, 520, 614, 646
HinfI GANTC 1 cut(s) 409
HpaII CCGG 2 cut(s) 382, 469
HphI GGTGA 2 cut(s) 119, 409
Hpy166II GTNNAC 2 cut(s) 522, 556
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 5 cut(s) 167, 535, 683, 733, 756
Hpy8I GTNNAC 2 cut(s) 522, 556
HpyAV CCTTC 2 cut(s) 247, 645
HpyCH4III ACNGT 2 cut(s) 490, 560
HpyCH4V TGCA 7 cut(s) 29, 61, 104, 124, 320, 556, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 117
HpyF3I CTNAG 2 cut(s) 508, 529
Hsp92II CATG 4 cut(s) 134, 520, 614, 646
Kzo9I GATC 3 cut(s) 163, 310, 598
LmnI GCTCC 2 cut(s) 241, 385
Lsp1109I GCAGC 2 cut(s) 256, 301
LweI GCATC 3 cut(s) 91, 133, 700
MaeI CTAG 2 cut(s) 323, 714
MaeIII GTNAC 2 cut(s) 200, 716
MalI GATC 3 cut(s) 165, 312, 600
MbiI CCGCTC 1 cut(s) 388
MboI GATC 3 cut(s) 163, 310, 598
MboII GAAGA 4 cut(s) 550, 632, 635, 748
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 2 cut(s) 476, 677
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 7 cut(s) 151, 270, 348, 449, 524, 538, 600
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 3 cut(s) 75, 150, 362
MslI CAYNNNNRTG 2 cut(s) 129, 196
MspI CCGG 2 cut(s) 382, 469
MspR9I CCNGG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 576
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 117
NciI CCSGG 1 cut(s) 383
NdeII GATC 3 cut(s) 163, 310, 598
NlaIII CATG 4 cut(s) 134, 520, 614, 646
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 646
PaqCI CACCTGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 513
PctI GAATGC 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 5 cut(s) 10, 246, 291, 390, 748
PshAI GACNNNNGTC 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 2 cut(s) 221, 471
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 3 cut(s) 402, 467, 521
RsaNI GTAC 3 cut(s) 401, 466, 520
RseI CAYNNNNRTG 2 cut(s) 129, 196
SaqAI TTAA 3 cut(s) 75, 150, 362
SatI GCNGC 5 cut(s) 9, 245, 290, 389, 747
Sau3AI GATC 3 cut(s) 163, 310, 598
Sau96I GGNCC 2 cut(s) 221, 471
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 1 cut(s) 558
SetI ASST 8 cut(s) 22, 109, 150, 162, 186, 249, 328, 530
SfaNI GCATC 3 cut(s) 91, 133, 700
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 2 cut(s) 129, 196
SmlI CTYRAG 2 cut(s) 236, 248
SmoI CTYRAG 2 cut(s) 236, 248
Sse9I AATT 2 cut(s) 476, 677
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 1 cut(s) 388
SspMI CTAG 2 cut(s) 323, 714
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 490, 560
TaqI TCGA 1 cut(s) 592
TasI AATT 2 cut(s) 476, 677
TatI WGTACW 2 cut(s) 400, 519
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 3 cut(s) 75, 150, 362
Tru9I TTAA 3 cut(s) 75, 150, 362
TscAI CASTG 2 cut(s) 63, 493
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 4 cut(s) 8, 244, 289, 746
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 186, 627
TspRI CASTG 2 cut(s) 63, 493
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 2 cut(s) 476, 677
XceI RCATGY 1 cut(s) 646
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 2 cut(s) 323, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.