Rh4AG167200

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003947
Physical Location & Seq
Forward (+)
42186245 .. 42194322
8078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG167200.1

Sequence Viewer

Length: 600 bp
ATGTCGGCTGCCGTGGAAGCTATATCTGCAACCAAAGATGTTGGTTTCAGTTCTCAAGTGCAGGAAGTGGAGTTTAAGAATGATAAGAACAGCAACAAGGATGCAGGTGGCGAACCAAGCCTGCATCACCATGACGAAAATGATGAGCTTAAAGATGAGGTTGATCCAGATGAAGAGGATGAGCTTGCCACATTCAAATGTGACAAAGAATTGGTTCTTGGCCCCAAGTTTTCTCTCAAGGAGCAGCTTGAGAAGGATAAAGATGACGAGAGTTTGAGGAAATGGAAGCAGCAACTTCTTGGGAGTGTTGATCTCTCTGCACTAGCTGTTGGAGAGAGTACAGAAGCAGATGTGAGGATTTTAAGTCTGACAATGATGTTCCGGGAGCGGCCTGAACTTGTGCTGCCGATTCCATTCACCAACAAACCAAAGAGCAGTCTTTTCACACTCAAAGGAGGAAGCAAGTACCGAACCAAATTCACATTCACTGTCTCCAAAAACATTGTCTCAGGCCTCATGTACACCACCTCAGTCTGGAAGACTGGTGTCAAAGCCACTCCGCCGCCTATTCTTATAAGATTATCAATTACAAGAACTTAA

Protein Analysis

199

Amino Acids

22.22

Weight (kDa)

5.37

Isoelectric Point (pI)

31.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 60 - 184 4.8e-40 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 575
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 388
AciI CCGC 3 cut(s) 388, 560, 563
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 3 cut(s) 340, 467, 521
AfiI CCNNNNNNNGG 1 cut(s) 534
AgsI TTSAA 1 cut(s) 196
AjuI GAANNNNNNNTTGG 4 cut(s) 201, 233, 467, 499
AluBI AGCT 5 cut(s) 20, 148, 184, 247, 326
AluI AGCT 5 cut(s) 20, 148, 184, 247, 326
Alw26I GTCTC 2 cut(s) 496, 511
AlwI GGATC 1 cut(s) 158
AoxI GGCC 3 cut(s) 220, 389, 511
ApeKI GCWGC 4 cut(s) 8, 244, 289, 403
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
Asp700I GAANNNNTTC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 119, 409
BbsI GAAGAC 1 cut(s) 545
BbvI GCAGC 3 cut(s) 256, 301, 390
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 2 cut(s) 496, 511
BfaI CTAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 6 cut(s) 9, 245, 290, 389, 404, 563
BlsI GCNGC 6 cut(s) 10, 246, 291, 390, 405, 564
Bme1390I CCNGG 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 383
BmsI GCATC 2 cut(s) 91, 133
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 545
BpuEI CTTGAG 3 cut(s) 39, 221, 269
BpuMI CCSGG 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 12
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse1I ACTGG 1 cut(s) 547
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 2 cut(s) 106, 184
BseLI CCNNNNNNNGG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 522, 543
BseNI ACTGG 1 cut(s) 547
BseXI GCAGC 3 cut(s) 256, 301, 390
BsgI GTGCAG 2 cut(s) 80, 303
BshFI GGCC 3 cut(s) 222, 391, 513
BsiSI CCGG 1 cut(s) 382
BslI CCNNNNNNNGG 1 cut(s) 534
BsmAI GTCTC 2 cut(s) 496, 511
BsnI GGCC 3 cut(s) 222, 391, 513
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 2 cut(s) 163, 310
BspACI CCGC 3 cut(s) 388, 560, 563
BspANI GGCC 3 cut(s) 222, 391, 513
BspCNI CTCAG 2 cut(s) 521, 542
BspLI GGNNCC 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 388
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 2 cut(s) 163, 310
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 1 cut(s) 168
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 122, 186
BstDEI CTNAG 2 cut(s) 508, 529
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 106, 184
BstKTI GATC 2 cut(s) 166, 313
BstMAI GTCTC 2 cut(s) 496, 511
BstMBI GATC 2 cut(s) 163, 310
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 117
BstPAI GACNNNNGTC 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 256, 301, 390
BstV2I GAAGAC 1 cut(s) 545
BsuRI GGCC 3 cut(s) 222, 391, 513
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 106, 184
BtsIMutI CAGTG 1 cut(s) 486
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 122, 186
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 339, 466, 520
CviAII CATG 2 cut(s) 131, 517
CviQI GTAC 3 cut(s) 339, 466, 520
DdeI CTNAG 2 cut(s) 508, 529
DpnI GATC 2 cut(s) 165, 312
DpnII GATC 2 cut(s) 163, 310
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 513
FaeI CATG 2 cut(s) 134, 520
FaiI YATR 4 cut(s) 23, 132, 518, 575
FatI CATG 2 cut(s) 130, 516
Fnu4HI GCNGC 6 cut(s) 9, 245, 290, 389, 404, 563
FokI GGATG 2 cut(s) 113, 191
Fsp4HI GCNGC 6 cut(s) 9, 245, 290, 389, 404, 563
FspBI CTAG 1 cut(s) 323
GluI GCNGC 6 cut(s) 9, 245, 290, 389, 404, 563
HaeIII GGCC 3 cut(s) 222, 391, 513
HapII CCGG 1 cut(s) 382
Hin1II CATG 2 cut(s) 134, 520
HinfI GANTC 1 cut(s) 409
HpaII CCGG 1 cut(s) 382
HphI GGTGA 2 cut(s) 119, 409
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 2 cut(s) 167, 535
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 5 cut(s) 29, 61, 104, 124, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 117
HpyF3I CTNAG 2 cut(s) 508, 529
Hsp92II CATG 2 cut(s) 134, 520
Kzo9I GATC 2 cut(s) 163, 310
LmnI GCTCC 2 cut(s) 241, 385
LpnPI CCDG 9 cut(s) 47, 90, 134, 180, 395, 405, 495, 520, 528
Lsp1109I GCAGC 3 cut(s) 256, 301, 390
LweI GCATC 2 cut(s) 91, 133
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 200
MalI GATC 2 cut(s) 165, 312
MbiI CCGCTC 1 cut(s) 388
MboI GATC 2 cut(s) 163, 310
MboII GAAGA 2 cut(s) 185, 550
MluCI AATT 3 cut(s) 209, 476, 585
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 7 cut(s) 151, 169, 270, 348, 449, 524, 538
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 4 cut(s) 75, 150, 362, 598
MslI CAYNNNNRTG 2 cut(s) 129, 196
MspI CCGG 1 cut(s) 382
MspR9I CCNGG 1 cut(s) 383
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 117
NciI CCSGG 1 cut(s) 383
NdeII GATC 2 cut(s) 163, 310
NlaIII CATG 2 cut(s) 134, 520
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 200
PaqCI CACCTGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 6 cut(s) 10, 246, 291, 390, 405, 564
PshAI GACNNNNGTC 1 cut(s) 545
PsiI TTATAA 1 cut(s) 575
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 221
RsaI GTAC 3 cut(s) 340, 467, 521
RsaNI GTAC 3 cut(s) 339, 466, 520
RseI CAYNNNNRTG 2 cut(s) 129, 196
SaqAI TTAA 4 cut(s) 75, 150, 362, 598
SatI GCNGC 6 cut(s) 9, 245, 290, 389, 404, 563
Sau3AI GATC 2 cut(s) 163, 310
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 383
SetI ASST 8 cut(s) 22, 109, 150, 162, 186, 249, 328, 530
SfaNI GCATC 2 cut(s) 91, 133
SmiMI CAYNNNNRTG 2 cut(s) 129, 196
SmlI CTYRAG 3 cut(s) 54, 236, 248
SmoI CTYRAG 3 cut(s) 54, 236, 248
Sse9I AATT 3 cut(s) 209, 476, 585
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 3 cut(s) 388, 560, 563
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 490
TasI AATT 3 cut(s) 209, 476, 585
TatI WGTACW 2 cut(s) 338, 519
TauI GCSGC 2 cut(s) 391, 565
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 4 cut(s) 75, 150, 362, 598
Tru9I TTAA 4 cut(s) 75, 150, 362, 598
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 4 cut(s) 8, 244, 289, 403
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 1 cut(s) 186
TspRI CASTG 1 cut(s) 493
XapI RAATTY 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.