FvH4_4g13460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002415
Physical Location & Seq
Reverse (-)
17062533 .. 17066982
4450 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13460.t2

Sequence Viewer

Length: 906 bp
ATGGAAGACCCGCCTCCTTCTTCATCACCGTCCGACCTCACCACCCTCCACAGACAAGTGCTTGAGCTTGAAGCGCTTCTCGATCGAATCCGCCGAGACGATGATTCCGATTCACCGGCTTCAGATTCCCAGGGTTTACTCAAAGACTGCTTTCGGTTTCTCGAGAGTAGAGTTGAAAAGATAGTGTCTGATTGCTCTGACGTTGGTCTTTTAGGGGATCAAGATTTCGAGGCATACGTGGGACGTTTCGAGGAGGAGCTTAGTTCTGTGGAGGCCGAGAGCGCTAAGGTTTCTGATGAAATTGAGGGTCTTGTTAGAACCCATGAGGAAGATTTGAATCGGCTCAATGTTGATATTGGGCAGGTGAAATGTTCGTTGGAATTTGTTGGATTGCAGGATCTGGACAAAGAAAAACTGGTTGCAGATGTTGATATATATGGAAAATATCAGTTGGATCCAATGGATGTGAATGAAGAAAAATTCAAGCTACTGGAACTTGAAAATCAGATTGACATGAATCATATAATATTGAAGTCTCTACAAGATTTGGAACATAAACTTAAATGGCTTGACGCCACTGAACAGTTTGAAGATGCATTTTCAGGTATCAAGGTCATTTCATTTGAGGAAAGCTGTATTAGGTTATCACTTAGTACGTATGTTCCAAAGTCAAAAGGTCTGTCCCAACAAATAATTGAAGATACCACCTCTGTGAATCACGAGCTACTAATTGAACTGGTGGAGGGGACTATGGACTTGAGCGATGCTGAGATTTTTCCAAATGATGTATGTATAAGAGATATTCTTGATGCTGCGAAGTCTTTGAGTAAATCTTCATTGCAGTGGTTCGTAACGAAAGTGCAAGACAAAATTGTTAAGCAGCATGGGGCGAATAGCGGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

33.94

Weight (kDa)

4.42

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 352
AciI CCGC 3 cut(s) 11, 91, 897
AclWI GGATC 4 cut(s) 225, 405, 449, 462
AcsI RAATTY 2 cut(s) 380, 479
AcuI CTGAAG 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 1 cut(s) 655
AfeI AGCGCT 2 cut(s) 75, 283
AgsI TTSAA 9 cut(s) 71, 176, 337, 484, 500, 532, 590, 698, 734
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 710
AluBI AGCT 5 cut(s) 67, 259, 487, 633, 724
AluI AGCT 5 cut(s) 67, 259, 487, 633, 724
Alw26I GTCTC 2 cut(s) 90, 540
AlwI GGATC 4 cut(s) 225, 405, 449, 462
AlwNI CAGNNNCTG 1 cut(s) 400
Ama87I CYCGRG 1 cut(s) 161
Aor51HI AGCGCT 2 cut(s) 75, 283
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 2 cut(s) 812, 880
ApoI RAATTY 2 cut(s) 380, 479
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
Asp700I GAANNNNTTC 1 cut(s) 75
AspLEI GCGC 2 cut(s) 76, 284
AsuHPI GGTGA 4 cut(s) 18, 31, 105, 376
AvaI CYCGRG 1 cut(s) 161
BamHI GGATCC 1 cut(s) 454
BauI CACGAG 1 cut(s) 719
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 799, 892
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 90, 540
BfoI RGCGCY 2 cut(s) 77, 285
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 2 cut(s) 813, 881
BlsI GCNGC 2 cut(s) 814, 882
Bme1390I CCNGG 1 cut(s) 131
BmeT110I CYCGRG 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 3 cut(s) 583, 754, 799
BoxI GACNNNNGTC 1 cut(s) 204
BpiI GAAGAC 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 83, 778
BsaAI YACGTR 2 cut(s) 238, 657
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 129, 130
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 3 cut(s) 420, 495, 741
Bse3DI GCAATG 1 cut(s) 836
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 129, 130
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 336
BseMI GCAATG 1 cut(s) 836
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 3 cut(s) 420, 495, 741
BseRI GAGGAG 2 cut(s) 266, 269
BseXI GCAGC 2 cut(s) 799, 892
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 275
BsiEI CGRYCG 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 3 cut(s) 255, 667, 760
BsmAI GTCTC 2 cut(s) 90, 540
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 3 cut(s) 255, 667, 760
BsnI GGCC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 161
Bsp143I GATC 4 cut(s) 82, 217, 397, 454
BspACI CCGC 3 cut(s) 11, 91, 897
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 760
BspLI GGNNCC 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 4 cut(s) 225, 405, 449, 462
BsrDI GCAATG 1 cut(s) 836
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 3 cut(s) 420, 495, 741
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 2 cut(s) 129, 130
BssMI GATC 4 cut(s) 82, 217, 397, 454
BssNI GRCGYC 1 cut(s) 573
BssSI CACGAG 1 cut(s) 719
Bst2BI CACGAG 1 cut(s) 719
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 30, 585
BstACI GRCGYC 1 cut(s) 573
BstBAI YACGTR 2 cut(s) 238, 657
BstDEI CTNAG 4 cut(s) 260, 285, 650, 768
BstF5I GGATG 1 cut(s) 469
BstH2I RGCGCY 2 cut(s) 77, 285
BstHHI GCGC 2 cut(s) 76, 284
BstKTI GATC 4 cut(s) 85, 220, 400, 457
BstMAI GTCTC 2 cut(s) 90, 540
BstMBI GATC 4 cut(s) 82, 217, 397, 454
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 73, 281
BstNI CCWGG 1 cut(s) 131
BstPAI GACNNNNGTC 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 129
BstSNI TACGTA 1 cut(s) 657
BstV1I GCAGC 2 cut(s) 799, 892
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 2 cut(s) 397, 454
BstYI RGATCY 2 cut(s) 397, 454
BsuRI GGCC 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 2 cut(s) 576, 848
BveI ACCTGC 1 cut(s) 352
CaiI CAGNNNCTG 1 cut(s) 400
CfoI GCGC 2 cut(s) 76, 284
Cfr10I RCCGGY 1 cut(s) 115
CseI GACGC 1 cut(s) 581
Csp6I GTAC 1 cut(s) 654
CviAII CATG 3 cut(s) 323, 514, 884
CviJI RGCY 9 cut(s) 67, 119, 259, 275, 343, 487, 568, 633, 724
CviKI_1 RGCY 9 cut(s) 67, 119, 259, 275, 343, 487, 568, 633, 724
CviQI GTAC 1 cut(s) 654
DdeI CTNAG 4 cut(s) 260, 285, 650, 768
DpnI GATC 4 cut(s) 84, 219, 399, 456
DpnII GATC 4 cut(s) 82, 217, 397, 454
EciI GGCGGA 1 cut(s) 80
Eco105I TACGTA 1 cut(s) 657
Eco47III AGCGCT 2 cut(s) 75, 283
Eco57I CTGAAG 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 598
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 3 cut(s) 326, 517, 887
FaqI GGGAC 3 cut(s) 255, 667, 760
FatI CATG 3 cut(s) 322, 513, 883
FauI CCCGC 2 cut(s) 18, 890
Fnu4HI GCNGC 2 cut(s) 813, 881
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 813, 881
GlaI GCGC 2 cut(s) 75, 283
GluI GCNGC 2 cut(s) 813, 881
HaeII RGCGCY 2 cut(s) 77, 285
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 116
HgaI GACGC 1 cut(s) 581
HhaI GCGC 2 cut(s) 76, 284
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 3 cut(s) 326, 517, 887
Hin6I GCGC 2 cut(s) 74, 282
HinP1I GCGC 2 cut(s) 74, 282
HinfI GANTC 7 cut(s) 87, 104, 110, 125, 337, 517, 715
HpaII CCGG 1 cut(s) 116
HphI GGTGA 4 cut(s) 18, 31, 105, 376
Hpy166II GTNNAC 1 cut(s) 137
Hpy188I TCNGA 7 cut(s) 34, 109, 124, 190, 199, 295, 507
Hpy188III TCNNGA 7 cut(s) 80, 161, 163, 221, 401, 719, 806
Hpy8I GTNNAC 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 27
HpyCH4III ACNGT 2 cut(s) 30, 585
HpyCH4IV ACGT 4 cut(s) 201, 237, 244, 656
HpyCH4V TGCA 5 cut(s) 394, 422, 596, 841, 862
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 281
HpyF3I CTNAG 4 cut(s) 260, 285, 650, 768
HpySE526I ACGT 4 cut(s) 201, 237, 244, 656
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 3 cut(s) 326, 517, 887
HspAI GCGC 2 cut(s) 74, 282
Kzo9I GATC 4 cut(s) 82, 217, 397, 454
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 2 cut(s) 799, 892
LweI GCATC 3 cut(s) 583, 754, 799
MaeII ACGT 4 cut(s) 201, 237, 244, 656
MaeIII GTNAC 1 cut(s) 850
MalI GATC 4 cut(s) 84, 219, 399, 456
MboI GATC 4 cut(s) 82, 217, 397, 454
MboII GAAGA 7 cut(s) 12, 17, 341, 485, 602, 710, 825
MflI RGATCY 2 cut(s) 397, 454
MluCI AATT 6 cut(s) 300, 380, 479, 693, 729, 870
MmeI TCCRAC 4 cut(s) 57, 357, 367, 432
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 75
MseI TTAA 2 cut(s) 561, 876
MslI CAYNNNNRTG 2 cut(s) 710, 841
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 2 cut(s) 73, 281
NdeII GATC 4 cut(s) 82, 217, 397, 454
NlaIII CATG 3 cut(s) 326, 517, 887
NlaIV GGNNCC 1 cut(s) 456
NmeAIII GCCGAG 2 cut(s) 119, 301
NsiI ATGCAT 1 cut(s) 598
OliI CACNNNNGTG 1 cut(s) 710
PaeR7I CTCGAG 1 cut(s) 161
PaqCI CACCTGC 1 cut(s) 352
PasI CCCWGGG 1 cut(s) 130
PcsI WCGNNNNNNNCGW 3 cut(s) 91, 105, 234
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 7 cut(s) 87, 104, 110, 125, 337, 517, 715
PkrI GCNGC 2 cut(s) 814, 882
Ple19I CGATCG 1 cut(s) 85
Ppu21I YACGTR 2 cut(s) 238, 657
PshAI GACNNNNGTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 2 cut(s) 397, 454
PvuI CGATCG 1 cut(s) 85
RsaI GTAC 1 cut(s) 655
RsaNI GTAC 1 cut(s) 654
RseI CAYNNNNRTG 2 cut(s) 710, 841
SaqAI TTAA 2 cut(s) 561, 876
SatI GCNGC 2 cut(s) 813, 881
Sau3AI GATC 4 cut(s) 82, 217, 397, 454
ScrFI CCNGG 1 cut(s) 131
SfaNI GCATC 3 cut(s) 583, 754, 799
Sfr274I CTCGAG 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 710, 841
SmlI CTYRAG 3 cut(s) 62, 161, 757
SmoI CTYRAG 3 cut(s) 62, 161, 757
SnaBI TACGTA 1 cut(s) 657
Sse9I AATT 6 cut(s) 300, 380, 479, 693, 729, 870
SsiI CCGC 3 cut(s) 11, 91, 897
SspI AATATT 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 30, 585
TaiI ACGT 4 cut(s) 204, 240, 247, 659
TaqI TCGA 5 cut(s) 81, 85, 162, 228, 249
TasI AATT 6 cut(s) 300, 380, 479, 693, 729, 870
TfiI GAWTC 7 cut(s) 87, 104, 110, 125, 337, 517, 715
Tru1I TTAA 2 cut(s) 561, 876
Tru9I TTAA 2 cut(s) 561, 876
TscAI CASTG 2 cut(s) 583, 848
TseI GCWGC 2 cut(s) 812, 880
TspDTI ATGAA 6 cut(s) 12, 312, 486, 530, 609, 825
TspRI CASTG 2 cut(s) 583, 848
XapI RAATTY 2 cut(s) 380, 479
XhoI CTCGAG 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 75
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.