RchiOBHm_Chr4g0414021

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002415
Physical Location & Seq
Reverse (-)
38375474 .. 38380452
4979 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38447

Sequence Viewer

Length: 906 bp
ATGGAAGACCCGGTTCCTTCTTCATCAGCCCCACCACCCGACCTCACCACCATTCACAGACGAGTGCTTGAGCTCGAAGAGATTCTCGAGCGGTGCCGCCGAGACGATGATTCCGAATCACCTACTGCAGATTCTCAGGATTTACTTAACGACTGCGCTGTGTTTCTCCGGAGTAGAGTTGAAAAGATAGTATCTGATTGCTCTGATGTTGGTCTGTTAGGGGATCAAGATTTTGAAGCATATATGGAACGTTTTGAGCAGGAGCTTAGCTCCGTGGAGGCCGAGAGCGCTAAGGTTTCTGATGAAGTTGAGGGTCTTGTTCGAACCCATGAGGAAGATTTTAATCGGCTTAATGTTGATATTGGGCAGTTGAAATGTTCATTGGATTTTGTTGGATCGCAGGATCTGGAGAAAGCAAAACTGGGTGCAGATGCTGATTGCCCTTCCTATGGAAAAGATAATGAAGACAAATTCAAGCTACTGGAACTTGAATATCAGATTGACAAGAATCATATAATTTTGAAGTCCCTACAAGATTTAGAATGCAAACTTAAATGGCTTGATGCCACCGAACAGTTTGAGGATGCATTTTCGGGTCTCATGGTCATTTCTTTTGAGGAAAACTGTATTAGGTTGTCACTTAGGACATACATTCCAAAGTCAAAAGGTCTGTCCCAACAAATAATTGAAGATACCACATCGGTGAACCATGAGCTACTCATTGAACTAATGGAAGGGACTATGGACTTGAGGAATGTTGAGATTTTTCCAAATGATGTATGTGTAAGAGATATTCTTGGTGCTGCAAAGTCTTTGAGTAAATCTTCATTGCAGTGGTTCGTAACAAAAGTGCAGGACAAAATCGTTAAGCACCATGGGGCGTCTAGTTGGAAAGAGTGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

34.13

Weight (kDa)

4.56

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 93
AccBSI CCGCTC 1 cut(s) 91
AccIII TCCGGA 1 cut(s) 168
AciI CCGC 2 cut(s) 91, 97
AclI AACGTT 1 cut(s) 250
AclWI GGATC 3 cut(s) 231, 403, 411
AcsI RAATTY 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 881
AfeI AGCGCT 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 8 cut(s) 182, 236, 373, 475, 491, 523, 689, 725
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AleI CACNNNNGTG 1 cut(s) 701
AluBI AGCT 5 cut(s) 73, 265, 270, 478, 715
AluI AGCT 5 cut(s) 73, 265, 270, 478, 715
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 2 cut(s) 96, 602
AlwI GGATC 3 cut(s) 231, 403, 411
AlwNI CAGNNNCTG 2 cut(s) 406, 434
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 168
Aor51HI AGCGCT 1 cut(s) 289
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 803
ApoI RAATTY 1 cut(s) 470
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
Asp700I GAANNNNTTC 1 cut(s) 81
AspLEI GCGC 2 cut(s) 158, 290
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 37, 111, 715
AsuII TTCGAA 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 86
BanI GGYRCC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 2 cut(s) 12, 471
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 790
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 2 cut(s) 96, 602
BfaI CTAG 1 cut(s) 885
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 291
BisI GCNGC 2 cut(s) 97, 804
BlpI GCTNAGC 1 cut(s) 266
BlsI GCNGC 2 cut(s) 98, 805
Bme1390I CCNGG 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 86
BmiI GGNNCC 2 cut(s) 15, 95
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 3 cut(s) 421, 553, 574
BmuI ACTGGG 1 cut(s) 431
BpiI GAAGAC 2 cut(s) 12, 471
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 291
Bpu1102I GCTNAGC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 322
BpuEI CTTGAG 2 cut(s) 89, 769
BpuMI CCSGG 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 881
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 273, 874
BsaWI WCCGGW 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 426, 486
Bse3DI GCAATG 1 cut(s) 827
Bse8I GATNNNNATC 1 cut(s) 342
BseAI TCCGGA 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 273, 874
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 2 cut(s) 426, 486
BseXI GCAGC 1 cut(s) 790
BsgI GTGCAG 2 cut(s) 447, 872
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 2 cut(s) 11, 169
BslFI GGGAC 3 cut(s) 511, 658, 751
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 2 cut(s) 96, 602
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 3 cut(s) 511, 658, 751
BsmI GAATGC 1 cut(s) 548
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 75
Bsp13I TCCGGA 1 cut(s) 168
Bsp143I GATC 3 cut(s) 223, 395, 403
Bsp1720I GCTNAGC 1 cut(s) 266
Bsp19I CCATGG 1 cut(s) 874
BspACI CCGC 2 cut(s) 91, 97
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 1 cut(s) 148
BspEI TCCGGA 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 15, 95
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 3 cut(s) 231, 403, 411
BspT104I TTCGAA 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 93
BspTNI GGTCTC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 827
BsrI ACTGG 2 cut(s) 426, 486
BssECI CCNNGG 2 cut(s) 273, 874
BssMI GATC 3 cut(s) 223, 395, 403
BssNI GRCGYC 1 cut(s) 881
BssT1I CCWWGG 1 cut(s) 874
Bst4CI ACNGT 2 cut(s) 576, 626
Bst6I CTCTTC 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 881
BstBI TTCGAA 1 cut(s) 322
BstDEI CTNAG 4 cut(s) 135, 266, 291, 641
BstDSI CCRYGG 2 cut(s) 273, 874
BstF5I GGATG 1 cut(s) 589
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 158, 290
BstKTI GATC 3 cut(s) 226, 398, 406
BstMAI GTCTC 2 cut(s) 96, 602
BstMBI GATC 3 cut(s) 223, 395, 403
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 790
BstV2I GAAGAC 2 cut(s) 12, 471
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 2 cut(s) 273, 874
BtsCI GGATG 1 cut(s) 589
BtsI GCAGTG 1 cut(s) 839
BtsIMutI CAGTG 1 cut(s) 839
CaiI CAGNNNCTG 2 cut(s) 406, 434
CfoI GCGC 2 cut(s) 158, 290
CseI GACGC 1 cut(s) 870
CviAII CATG 4 cut(s) 329, 601, 710, 875
CviJI RGCY 9 cut(s) 29, 73, 265, 270, 281, 349, 478, 559, 715
CviKI_1 RGCY 9 cut(s) 29, 73, 265, 270, 281, 349, 478, 559, 715
DdeI CTNAG 4 cut(s) 135, 266, 291, 641
DpnI GATC 3 cut(s) 225, 397, 405
DpnII GATC 3 cut(s) 223, 395, 403
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 874
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 602
Eco47III AGCGCT 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 874
EcoT22I ATGCAT 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 874
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 4 cut(s) 332, 604, 713, 878
FaqI GGGAC 3 cut(s) 511, 658, 751
FatI CATG 4 cut(s) 328, 600, 709, 874
Fnu4HI GCNGC 2 cut(s) 97, 804
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 97, 804
FspBI CTAG 1 cut(s) 885
GlaI GCGC 2 cut(s) 157, 289
GluI GCNGC 2 cut(s) 97, 804
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 1 cut(s) 281
HapII CCGG 2 cut(s) 11, 169
HgaI GACGC 1 cut(s) 870
HhaI GCGC 2 cut(s) 158, 290
Hin1I GRCGYC 1 cut(s) 881
Hin1II CATG 4 cut(s) 332, 604, 713, 878
Hin6I GCGC 2 cut(s) 156, 288
HinP1I GCGC 2 cut(s) 156, 288
HinfI GANTC 5 cut(s) 82, 110, 116, 131, 508
HpaII CCGG 2 cut(s) 11, 169
HphI GGTGA 3 cut(s) 37, 111, 715
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 5 cut(s) 115, 196, 205, 301, 498
Hpy188III TCNNGA 5 cut(s) 86, 137, 169, 227, 407
Hpy8I GTNNAC 1 cut(s) 706
HpyAV CCTTC 3 cut(s) 27, 453, 728
HpyCH4III ACNGT 2 cut(s) 576, 626
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 8 cut(s) 128, 428, 546, 587, 806, 832, 853, 900
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 4 cut(s) 135, 266, 291, 641
HpySE526I ACGT 1 cut(s) 250
Hsp92I GRCGYC 1 cut(s) 881
Hsp92II CATG 4 cut(s) 332, 604, 713, 878
HspAI GCGC 2 cut(s) 156, 288
Kpn2I TCCGGA 1 cut(s) 168
Kzo9I GATC 3 cut(s) 223, 395, 403
LmnI GCTCC 2 cut(s) 262, 275
LpnPI CCDG 9 cut(s) 24, 122, 182, 245, 386, 392, 407, 467, 839
Lsp1109I GCAGC 1 cut(s) 790
LweI GCATC 3 cut(s) 421, 553, 574
MaeI CTAG 1 cut(s) 885
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 2 cut(s) 636, 841
MalI GATC 3 cut(s) 225, 397, 405
MbiI CCGCTC 1 cut(s) 91
MboI GATC 3 cut(s) 223, 395, 403
MboII GAAGA 7 cut(s) 12, 17, 89, 347, 476, 701, 816
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 470, 516, 684
MmeI TCCRAC 2 cut(s) 373, 869
MnlI CCTC 7 cut(s) 53, 271, 304, 325, 574, 610, 744
Mph1103I ATGCAT 1 cut(s) 589
MroI TCCGGA 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 5 cut(s) 147, 342, 351, 552, 867
MslI CAYNNNNRTG 2 cut(s) 701, 832
MspI CCGG 2 cut(s) 11, 169
MspR9I CCNGG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 548
MwoI GCNNNNNNNGC 1 cut(s) 287
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 874
NdeII GATC 3 cut(s) 223, 395, 403
NlaIII CATG 4 cut(s) 332, 604, 713, 878
NlaIV GGNNCC 2 cut(s) 15, 95
NmeAIII GCCGAG 2 cut(s) 125, 307
NmuCI GTSAC 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 589
NspV TTCGAA 1 cut(s) 322
OliI CACNNNNGTG 1 cut(s) 701
PaeR7I CTCGAG 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 548
PdmI GAANNNNTTC 1 cut(s) 81
PfeI GAWTC 5 cut(s) 82, 110, 116, 131, 508
PkrI GCNGC 2 cut(s) 98, 805
Psp124BI GAGCTC 1 cut(s) 75
Psp1406I AACGTT 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 15, 95
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 2 cut(s) 406, 434
PsuI RGATCY 1 cut(s) 403
RseI CAYNNNNRTG 2 cut(s) 701, 832
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 5 cut(s) 147, 342, 351, 552, 867
SatI GCNGC 2 cut(s) 97, 804
Sau3AI GATC 3 cut(s) 223, 395, 403
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 3 cut(s) 421, 553, 574
SfcI CTRYAG 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 701, 832
SmlI CTYRAG 3 cut(s) 68, 86, 748
SmoI CTYRAG 3 cut(s) 68, 86, 748
Sse9I AATT 3 cut(s) 470, 516, 684
SsiI CCGC 2 cut(s) 91, 97
SspMI CTAG 1 cut(s) 885
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 2 cut(s) 576, 626
TaiI ACGT 1 cut(s) 253
TaqI TCGA 3 cut(s) 75, 87, 322
TasI AATT 3 cut(s) 470, 516, 684
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 5 cut(s) 82, 110, 116, 131, 508
Tru1I TTAA 5 cut(s) 147, 342, 351, 552, 867
Tru9I TTAA 5 cut(s) 147, 342, 351, 552, 867
TscAI CASTG 1 cut(s) 839
TseFI GTSAC 1 cut(s) 636
TseI GCWGC 1 cut(s) 803
Tsp45I GTSAC 1 cut(s) 636
TspDTI ATGAA 5 cut(s) 12, 318, 369, 477, 816
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 1 cut(s) 839
XapI RAATTY 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 1 cut(s) 885
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.