Rh5DG373300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002415
Physical Location & Seq
Forward (+)
56522096 .. 56525259
3164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG373300.1

Sequence Viewer

Length: 480 bp
ATGGAAGACCCGGTTCCTTCTTCATCAGCCCCACCACCCAACCTCACCACCATTCACAAGATTCTCGAGCGATGCCGCCGAGATGATGATTCCGAATCACCTACTGCAGATTCTCAGGATTTACTTAACGACTGCGCTGTGTTTCTCCGGAGTAGTGTTGAAAAGATAGTAACTGATTGCTCTGATGTTGGTCTGTTGGGGGATCAAGATTTTGAAGCATATATGGGACGTTTTGAGCTGGAGCTTAGCTCCGTGGAGGCCGAGAGCGCTAAGGTGCATCTGTTGGAGAAGAGCTTTGAGACAGATAACAAGCTGGAGCCGGATGGTAAGACGCAGCTGGCCAAGAAGCTAGGGCTGCAGCCAAGACAGGTGGCTGTGTGGTTCCAGAATCATAGGGCTCGCTGGAAGACAAAGCAGCTCGAGCGCGATTATGATGTTCTAAACTACAGATGGTGTTGCTATATTGTACTTCCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

18.19

Weight (kDa)

4.91

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 90 - 136 1.3e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 426
AccIII TCCGGA 1 cut(s) 147
AciI CCGC 1 cut(s) 76
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 339
AfaI GTAC 1 cut(s) 468
AfeI AGCGCT 1 cut(s) 268
AgsI TTSAA 2 cut(s) 161, 215
AluBI AGCT 8 cut(s) 238, 244, 249, 294, 313, 337, 349, 418
AluI AGCT 8 cut(s) 238, 244, 249, 294, 313, 337, 349, 418
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 210
Ama87I CYCGRG 2 cut(s) 65, 419
Aor13HI TCCGGA 1 cut(s) 147
Aor51HI AGCGCT 1 cut(s) 268
AoxI GGCC 2 cut(s) 258, 339
ApeKI GCWGC 4 cut(s) 334, 355, 358, 415
AspLEI GCGC 3 cut(s) 137, 269, 426
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 37, 90
AvaI CYCGRG 2 cut(s) 65, 419
BalI TGGCCA 1 cut(s) 341
BanII GRGCYC 1 cut(s) 400
BbsI GAAGAC 2 cut(s) 12, 413
BbvI GCAGC 4 cut(s) 342, 346, 370, 427
BccI CCATC 2 cut(s) 317, 444
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 3 cut(s) 105, 356, 445
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 5 cut(s) 76, 335, 356, 359, 416
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 5 cut(s) 77, 336, 357, 360, 417
Bme1390I CCNGG 1 cut(s) 11
BmeT110I CYCGRG 2 cut(s) 65, 419
BmiI GGNNCC 3 cut(s) 15, 318, 383
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 2 cut(s) 62, 286
BpiI GAAGAC 2 cut(s) 12, 413
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 2 cut(s) 260, 335
Bpu10I CCTNAGC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 147
BseAI TCCGGA 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 128
BseXI GCAGC 4 cut(s) 342, 346, 370, 427
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 2 cut(s) 260, 341
BsiHKCI CYCGRG 2 cut(s) 65, 419
BsiSI CCGG 3 cut(s) 11, 148, 320
BslFI GGGAC 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 240
BsnI GGCC 2 cut(s) 260, 341
BsoBI CYCGRG 2 cut(s) 65, 419
Bsp1286I GDGCHC 1 cut(s) 400
Bsp13I TCCGGA 1 cut(s) 147
Bsp143I GATC 1 cut(s) 202
Bsp1720I GCTNAGC 1 cut(s) 245
BspACI CCGC 1 cut(s) 76
BspANI GGCC 2 cut(s) 260, 341
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 147
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 3 cut(s) 15, 318, 383
BspMAI CTGCAG 2 cut(s) 109, 360
BspPI GGATC 1 cut(s) 210
BspQI GCTCTTC 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 1 cut(s) 202
Bst6I CTCTTC 1 cut(s) 284
BstC8I GCNNGC 2 cut(s) 339, 400
BstDEI CTNAG 3 cut(s) 114, 245, 270
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 3 cut(s) 137, 269, 426
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 3 cut(s) 266, 355, 421
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 3 cut(s) 105, 356, 445
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 342, 346, 370, 427
BstV2I GAAGAC 2 cut(s) 12, 413
BsuRI GGCC 2 cut(s) 260, 341
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 339, 400
CfoI GCGC 3 cut(s) 137, 269, 426
CseI GACGC 1 cut(s) 340
Csp6I GTAC 1 cut(s) 467
CspCI CAANNNNNGTGG 2 cut(s) 351, 386
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 3 cut(s) 114, 245, 270
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco24I GRGCYC 1 cut(s) 400
Eco47III AGCGCT 1 cut(s) 268
Eco88I CYCGRG 2 cut(s) 65, 419
EcoT38I GRGCYC 1 cut(s) 400
FaiI YATR 6 cut(s) 220, 222, 224, 393, 432, 462
FaqI GGGAC 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 76, 335, 356, 359, 416
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 5 cut(s) 76, 335, 356, 359, 416
FspBI CTAG 1 cut(s) 350
GlaI GCGC 3 cut(s) 136, 268, 425
GluI GCNGC 5 cut(s) 76, 335, 356, 359, 416
GsuI CTGGAG 2 cut(s) 260, 335
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 2 cut(s) 260, 341
HapII CCGG 3 cut(s) 11, 148, 320
HgaI GACGC 1 cut(s) 340
HhaI GCGC 3 cut(s) 137, 269, 426
Hin6I GCGC 3 cut(s) 135, 267, 424
HinP1I GCGC 3 cut(s) 135, 267, 424
HinfI GANTC 5 cut(s) 61, 89, 95, 110, 388
HpaII CCGG 3 cut(s) 11, 148, 320
HphI GGTGA 2 cut(s) 37, 90
Hpy188I TCNGA 3 cut(s) 94, 184, 479
Hpy188III TCNNGA 5 cut(s) 65, 116, 148, 206, 385
HpyAV CCTTC 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 3 cut(s) 107, 277, 358
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 355, 421
HpyF3I CTNAG 3 cut(s) 114, 245, 270
HpySE526I ACGT 1 cut(s) 229
HspAI GCGC 3 cut(s) 135, 267, 424
Kpn2I TCCGGA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 202
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 3 cut(s) 241, 254, 316
Lsp1109I GCAGC 4 cut(s) 342, 346, 370, 427
LweI GCATC 2 cut(s) 62, 286
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 1 cut(s) 169
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 4 cut(s) 12, 17, 301, 418
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 2 cut(s) 53, 250
Mox20I TGGCCA 1 cut(s) 341
MroI TCCGGA 1 cut(s) 147
MscI TGGCCA 1 cut(s) 341
MseI TTAA 1 cut(s) 126
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 3 cut(s) 11, 148, 320
MspR9I CCNGG 1 cut(s) 11
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 266, 355, 421
NciI CCSGG 1 cut(s) 11
NdeII GATC 1 cut(s) 202
NlaIV GGNNCC 3 cut(s) 15, 318, 383
NmeAIII GCCGAG 2 cut(s) 104, 286
PaeR7I CTCGAG 2 cut(s) 65, 419
PciSI GCTCTTC 1 cut(s) 284
PfeI GAWTC 5 cut(s) 61, 89, 95, 110, 388
PkrI GCNGC 5 cut(s) 77, 336, 357, 360, 417
PspN4I GGNNCC 3 cut(s) 15, 318, 383
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 2 cut(s) 109, 360
PvuII CAGCTG 1 cut(s) 337
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 5 cut(s) 76, 335, 356, 359, 416
Sau3AI GATC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 2 cut(s) 62, 286
SfcI CTRYAG 3 cut(s) 105, 356, 445
Sfr274I CTCGAG 2 cut(s) 65, 419
SlaI CTCGAG 2 cut(s) 65, 419
SmlI CTYRAG 2 cut(s) 65, 419
SmoI CTYRAG 2 cut(s) 65, 419
SsiI CCGC 1 cut(s) 76
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 1 cut(s) 9
TaiI ACGT 1 cut(s) 232
TaqI TCGA 2 cut(s) 66, 420
TatI WGTACW 1 cut(s) 466
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 5 cut(s) 61, 89, 95, 110, 388
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TseI GCWGC 4 cut(s) 334, 355, 358, 415
TspDTI ATGAA 1 cut(s) 12
TspGWI ACGGA 1 cut(s) 241
XhoI CTCGAG 2 cut(s) 65, 419
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.