Rh4DG179100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002415
Physical Location & Seq
Reverse (-)
35645506 .. 35646861
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG179100.1

Sequence Viewer

Length: 534 bp
ATGGAAGACCCAGTTCCTTCTTCATCAGCCCCACCCGACCTCACAACCATTCACAGACGAGTGCTTGAGCTCGAAGAGATTCTCCAGCGATGCCACCGAGACGATGATTCCGAATCACCTACTGCAGATTCTCAGGATTTACTTCACGAGTGCGCACTGTTTCTCCGGAGTAGAGTTGAAAAGATAGTATCTGATTGCTCTGATGTTGGTCTGTTGGGGGATCAAGATTTTGAAGCATATATGGGACGTTTTGAGCAGGAGCTTAGCTCCGTGGAGGCCGAGAGCGCTAAGGTTTCCGATGAAATTGAGGGTCTTGTTCGAACCCATGAGGAAGATTTTAATCGGGTCAATGTTGATATTGGGCAGTTGAAATGTTCATTGGATTTTGTTGGATCGCAGGATTTCCACACGAAGCACAATCTTATAGATGGGTGGTTTTCTGTGCTCATGTCCCTGAAATGGGTAGAAACACTCAGCATTACTGCTGGATATCCATTTCTCCAAATACTCTTCAATTGTAACTCTAATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

20.02

Weight (kDa)

4.41

Isoelectric Point (pI)

66.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 154
AccIII TCCGGA 1 cut(s) 165
AclWI GGATC 2 cut(s) 228, 400
AfeI AGCGCT 1 cut(s) 286
AfiI CCNNNNNNNGG 2 cut(s) 459, 460
AgsI TTSAA 4 cut(s) 179, 233, 370, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 3 cut(s) 70, 262, 267
AluI AGCT 3 cut(s) 70, 262, 267
Alw21I GWGCWC 2 cut(s) 72, 447
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 2 cut(s) 228, 400
Aor13HI TCCGGA 1 cut(s) 165
Aor51HI AGCGCT 1 cut(s) 286
AoxI GGCC 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 2 cut(s) 155, 287
AsuHPI GGTGA 1 cut(s) 108
AsuII TTCGAA 1 cut(s) 319
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 72, 447
BccI CCATC 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 93
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 288
BlpI GCTNAGC 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 5
BmsI GCATC 1 cut(s) 80
BmuI ACTGGG 1 cut(s) 5
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 263
Bpu14I TTCGAA 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 2 cut(s) 459, 460
Bse1I ACTGG 1 cut(s) 11
Bse8I GATNNNNATC 1 cut(s) 339
BseAI TCCGGA 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 2 cut(s) 459, 460
BseMII CTCAG 2 cut(s) 146, 487
BseNI ACTGG 1 cut(s) 11
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 72, 447
BsiSI CCGG 1 cut(s) 166
BslFI GGGAC 2 cut(s) 258, 436
BslI CCNNNNNNNGG 2 cut(s) 459, 460
BsmAI GTCTC 1 cut(s) 93
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 258, 436
BsnI GGCC 1 cut(s) 278
Bsp119I TTCGAA 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 72, 447
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 2 cut(s) 220, 392
Bsp1720I GCTNAGC 1 cut(s) 263
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 2 cut(s) 145, 486
BspEI TCCGGA 1 cut(s) 165
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 2 cut(s) 228, 400
BspT104I TTCGAA 1 cut(s) 319
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 2 cut(s) 220, 392
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 159
Bst6I CTCTTC 2 cut(s) 69, 515
BstBI TTCGAA 1 cut(s) 319
BstDEI CTNAG 4 cut(s) 132, 263, 288, 473
BstDSI CCRYGG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 155, 287
BstKTI GATC 2 cut(s) 223, 395
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 2 cut(s) 220, 392
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 123
BstV2I GAAGAC 1 cut(s) 12
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 270
BtgZI GCGATG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 155
CfoI GCGC 2 cut(s) 155, 287
CviAII CATG 2 cut(s) 326, 448
CviJI RGCY 5 cut(s) 29, 70, 262, 267, 278
CviKI_1 RGCY 5 cut(s) 29, 70, 262, 267, 278
DdeI CTNAG 4 cut(s) 132, 263, 288, 473
DpnI GATC 2 cut(s) 222, 394
DpnII GATC 2 cut(s) 220, 392
Eam1104I CTCTTC 2 cut(s) 69, 515
EarI CTCTTC 2 cut(s) 69, 515
Ecl136II GAGCTC 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 72
Eco32I GATATC 1 cut(s) 491
Eco47III AGCGCT 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoRV GATATC 1 cut(s) 491
EcoT38I GRGCYC 1 cut(s) 72
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 2 cut(s) 329, 451
FaiI YATR 6 cut(s) 238, 240, 242, 327, 425, 449
FaqI GGGAC 2 cut(s) 258, 436
FatI CATG 2 cut(s) 325, 447
FriOI GRGCYC 1 cut(s) 72
FspAI RTGCGCAY 1 cut(s) 154
FspI TGCGCA 1 cut(s) 154
GlaI GCGC 2 cut(s) 154, 286
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 166
HhaI GCGC 2 cut(s) 155, 287
Hin1II CATG 2 cut(s) 329, 451
Hin6I GCGC 2 cut(s) 153, 285
HinP1I GCGC 2 cut(s) 153, 285
HinfI GANTC 4 cut(s) 79, 107, 113, 128
HpaII CCGG 1 cut(s) 166
HphI GGTGA 1 cut(s) 108
Hpy188I TCNGA 4 cut(s) 112, 193, 202, 298
Hpy188III TCNNGA 4 cut(s) 134, 146, 166, 224
HpyAV CCTTC 1 cut(s) 27
HpyCH4III ACNGT 1 cut(s) 159
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpyF3I CTNAG 4 cut(s) 132, 263, 288, 473
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 2 cut(s) 329, 451
HspAI GCGC 2 cut(s) 153, 285
Kpn2I TCCGGA 1 cut(s) 165
Kzo9I GATC 2 cut(s) 220, 392
LmnI GCTCC 2 cut(s) 259, 272
LpnPI CCDG 8 cut(s) 24, 98, 119, 179, 242, 383, 467, 471
LweI GCATC 1 cut(s) 80
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 1 cut(s) 518
MalI GATC 2 cut(s) 222, 394
MboI GATC 2 cut(s) 220, 392
MboII GAAGA 5 cut(s) 12, 17, 86, 344, 502
MfeI CAATTG 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 72, 447
MluCI AATT 2 cut(s) 303, 514
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 4 cut(s) 50, 268, 301, 322
MroI TCCGGA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 1 cut(s) 339
MspI CCGG 1 cut(s) 166
MunI CAATTG 1 cut(s) 514
MwoI GCNNNNNNNGC 1 cut(s) 284
NdeII GATC 2 cut(s) 220, 392
NlaIII CATG 2 cut(s) 329, 451
NmeAIII GCCGAG 1 cut(s) 304
NsbI TGCGCA 1 cut(s) 154
NspV TTCGAA 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 4 cut(s) 79, 107, 113, 128
Psp124BI GAGCTC 1 cut(s) 72
PstI CTGCAG 1 cut(s) 127
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 1 cut(s) 339
Sau3AI GATC 2 cut(s) 220, 392
SduI GDGCHC 2 cut(s) 72, 447
SetI ASST 7 cut(s) 42, 72, 121, 250, 264, 269, 294
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 2 cut(s) 303, 514
SstI GAGCTC 1 cut(s) 72
TaaI ACNGT 1 cut(s) 159
TaiI ACGT 1 cut(s) 250
TaqI TCGA 2 cut(s) 72, 319
TasI AATT 2 cut(s) 303, 514
TfiI GAWTC 4 cut(s) 79, 107, 113, 128
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 162
TspDTI ATGAA 3 cut(s) 12, 315, 366
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.