Rh5BG357800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002415
Physical Location & Seq
Forward (+)
55770727 .. 55776246
5520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG357800.1

Sequence Viewer

Length: 492 bp
ATGAACTACCTACCTCATAGTTTCATACTGAAGAAAAGTTACCATTTACTACGAGTGCTTGAGTTCGAAGAGATTCTCGAGCGATGCCGCCGAGATGATGATTCCGAATCACCTACTGCAGATTCTCAGGATTTACTTAACGACTGCGCTGTGTTTCTCCGGAGTAGTGTTGAAAAGATAGTAACTGATTGCTCTGATGTTGGTCTGTTGGGGGATCAAGATTTTGAAGCATATATGGGACGTTTTGAGCTGGAGCTTAGCTCCGTGGAGGCCGAGAGCGCTAAGGTGCATCTGTTGGAGAAGAGCTTTGAGACAGATAACAAGCTGGAGCCGGATGGTAAGACGCAGCTGGCCAAGAAGCTAGGGCTGCAGCCAAGACAGGTGGCTGTGTGGTTCCAGAATCATAGGGCTCGCTGGAAGACAAAGCAGCTCGAGCGCGATTATGATGTTCTAAACTACAGATGGTGTTGCTATATTGTACTTCCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

19.08

Weight (kDa)

5.15

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 94 - 140 1.3e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 438
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 1 cut(s) 88
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 1 cut(s) 480
AfeI AGCGCT 1 cut(s) 280
AgsI TTSAA 2 cut(s) 173, 227
AluBI AGCT 8 cut(s) 250, 256, 261, 306, 325, 349, 361, 430
AluI AGCT 8 cut(s) 250, 256, 261, 306, 325, 349, 361, 430
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 1 cut(s) 222
Ama87I CYCGRG 2 cut(s) 77, 431
Aor13HI TCCGGA 1 cut(s) 159
Aor51HI AGCGCT 1 cut(s) 280
AoxI GGCC 2 cut(s) 270, 351
ApeKI GCWGC 4 cut(s) 346, 367, 370, 427
Asp700I GAANNNNTTC 1 cut(s) 72
AspLEI GCGC 3 cut(s) 149, 281, 438
AsuHPI GGTGA 1 cut(s) 102
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 2 cut(s) 77, 431
BalI TGGCCA 1 cut(s) 353
BanII GRGCYC 1 cut(s) 412
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 4 cut(s) 354, 358, 382, 439
BccI CCATC 2 cut(s) 329, 456
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 3 cut(s) 117, 368, 457
BfoI RGCGCY 1 cut(s) 282
BisI GCNGC 5 cut(s) 88, 347, 368, 371, 428
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 5 cut(s) 89, 348, 369, 372, 429
BmeT110I CYCGRG 2 cut(s) 77, 431
BmiI GGNNCC 2 cut(s) 330, 395
BmsI GCATC 2 cut(s) 74, 298
BpiI GAAGAC 1 cut(s) 425
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpmI CTGGAG 2 cut(s) 272, 347
Bpu10I CCTNAGC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 257
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 264
BsaWI WCCGGW 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 140
BseXI GCAGC 4 cut(s) 354, 358, 382, 439
Bsh1236I CGCG 1 cut(s) 438
BshFI GGCC 2 cut(s) 272, 353
BsiHKCI CYCGRG 2 cut(s) 77, 431
BsiSI CCGG 2 cut(s) 160, 332
BslFI GGGAC 1 cut(s) 252
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 2 cut(s) 272, 353
BsoBI CYCGRG 2 cut(s) 77, 431
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 412
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 1 cut(s) 214
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 1 cut(s) 88
BspANI GGCC 2 cut(s) 272, 353
BspCNI CTCAG 1 cut(s) 139
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 330, 395
BspMAI CTGCAG 2 cut(s) 121, 372
BspPI GGATC 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 296
BspT104I TTCGAA 1 cut(s) 66
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 1 cut(s) 214
Bst6I CTCTTC 2 cut(s) 63, 296
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 351, 412
BstDEI CTNAG 3 cut(s) 126, 257, 282
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 438
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 3 cut(s) 149, 281, 438
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 3 cut(s) 278, 367, 433
BstSFI CTRYAG 3 cut(s) 117, 368, 457
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 4 cut(s) 354, 358, 382, 439
BstV2I GAAGAC 1 cut(s) 425
BsuRI GGCC 2 cut(s) 272, 353
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 97
BtsCI GGATG 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 351, 412
CfoI GCGC 3 cut(s) 149, 281, 438
CseI GACGC 1 cut(s) 352
Csp6I GTAC 1 cut(s) 479
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 3 cut(s) 126, 257, 282
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 2 cut(s) 63, 296
EarI CTCTTC 2 cut(s) 63, 296
Eco24I GRGCYC 1 cut(s) 412
Eco47III AGCGCT 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 50
Eco88I CYCGRG 2 cut(s) 77, 431
EcoT38I GRGCYC 1 cut(s) 412
FaiI YATR 8 cut(s) 18, 26, 232, 234, 236, 405, 444, 474
FaqI GGGAC 1 cut(s) 252
Fnu4HI GCNGC 5 cut(s) 88, 347, 368, 371, 428
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 5 cut(s) 88, 347, 368, 371, 428
FspBI CTAG 1 cut(s) 362
GlaI GCGC 3 cut(s) 148, 280, 437
GluI GCNGC 5 cut(s) 88, 347, 368, 371, 428
GsuI CTGGAG 2 cut(s) 272, 347
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 2 cut(s) 272, 353
HapII CCGG 2 cut(s) 160, 332
HgaI GACGC 1 cut(s) 352
HhaI GCGC 3 cut(s) 149, 281, 438
Hin6I GCGC 3 cut(s) 147, 279, 436
HinP1I GCGC 3 cut(s) 147, 279, 436
HinfI GANTC 5 cut(s) 73, 101, 107, 122, 400
HpaII CCGG 2 cut(s) 160, 332
HphI GGTGA 1 cut(s) 102
Hpy188I TCNGA 3 cut(s) 106, 196, 491
Hpy188III TCNNGA 5 cut(s) 77, 128, 160, 218, 397
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 3 cut(s) 119, 289, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 367, 433
HpyF3I CTNAG 3 cut(s) 126, 257, 282
HpySE526I ACGT 1 cut(s) 241
HspAI GCGC 3 cut(s) 147, 279, 436
Kpn2I TCCGGA 1 cut(s) 159
Kzo9I GATC 1 cut(s) 214
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 3 cut(s) 253, 266, 328
LpnPI CCDG 9 cut(s) 113, 173, 236, 311, 335, 345, 365, 400, 410
Lsp1109I GCAGC 4 cut(s) 354, 358, 382, 439
LweI GCATC 2 cut(s) 74, 298
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 2 cut(s) 38, 181
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 4 cut(s) 43, 80, 313, 430
MhlI GDGCHC 1 cut(s) 412
MlsI TGGCCA 1 cut(s) 353
MluNI TGGCCA 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 2 cut(s) 24, 262
Mox20I TGGCCA 1 cut(s) 353
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 72
MscI TGGCCA 1 cut(s) 353
MseI TTAA 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 160, 332
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 3 cut(s) 278, 367, 433
NdeII GATC 1 cut(s) 214
NlaIV GGNNCC 2 cut(s) 330, 395
NmeAIII GCCGAG 2 cut(s) 116, 298
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 2 cut(s) 77, 431
PciSI GCTCTTC 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 5 cut(s) 73, 101, 107, 122, 400
PkrI GCNGC 5 cut(s) 89, 348, 369, 372, 429
PspN4I GGNNCC 2 cut(s) 330, 395
PspXI VCTCGAGB 1 cut(s) 431
PstI CTGCAG 2 cut(s) 121, 372
PvuII CAGCTG 1 cut(s) 349
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 5 cut(s) 88, 347, 368, 371, 428
Sau3AI GATC 1 cut(s) 214
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 2 cut(s) 74, 298
SfcI CTRYAG 3 cut(s) 117, 368, 457
Sfr274I CTCGAG 2 cut(s) 77, 431
SfuI TTCGAA 1 cut(s) 66
SlaI CTCGAG 2 cut(s) 77, 431
SmlI CTYRAG 3 cut(s) 59, 77, 431
SmoI CTYRAG 3 cut(s) 59, 77, 431
SsiI CCGC 1 cut(s) 88
SspMI CTAG 1 cut(s) 362
TaiI ACGT 1 cut(s) 244
TaqI TCGA 3 cut(s) 66, 78, 432
TatI WGTACW 1 cut(s) 478
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 5 cut(s) 73, 101, 107, 122, 400
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TseI GCWGC 4 cut(s) 346, 367, 370, 427
TspDTI ATGAA 2 cut(s) 13, 17
TspGWI ACGGA 1 cut(s) 253
XhoI CTCGAG 2 cut(s) 77, 431
XmnI GAANNNNTTC 1 cut(s) 72
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.