FvH4_4g18150

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005001
Physical Location & Seq
Reverse (-)
22086947 .. 22087765
819 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18150.t1

Sequence Viewer

Length: 450 bp
ATGTTTGCCTCACTAAACAAGGTTTCAGCTGAATTTAGAAGGCTTCTGAACCACATGATGTTGAACCGGGGTTCCTTCTTTGCTGTGGAATTTGATACCCTTTTTGACCCTGTGGTGGATGACATCAATGGCAGCCATGTGGGGATTGATCTCAACACTGTTGAGAGTGTGGCTTCTGTTAATTCTGGGGTTGATTTGGTGGGTGGGAAAGAGATTACTGCTTGGATTGAGTACAGAGATGATCTGAAATTGGTTCGGGTTGGGGTCGGGCATTCGCCGGAGAATAAGCCTCCGAGTCCGGTTATTGCGGCCCAGATTGACCTTTCCATGCAATTCAACGAGTTGGTGTATGTGGGCTTGACTGCTTCTAATGGGAAGGGCGGCTCTTCTGCCTACATTGTTAATAGTTGGAGGTTCAAGACTTTCAATGAGACTTGTATGAACTGGTAA

Protein Analysis

150

Amino Acids

16.43

Weight (kDa)

5.07

Isoelectric Point (pI)

23.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 23 - 141 3.7e-28 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 308, 381
AcsI RAATTY 2 cut(s) 32, 89
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 115
AgsI TTSAA 4 cut(s) 64, 337, 418, 427
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 1 cut(s) 29
AluI AGCT 1 cut(s) 29
Alw26I GTCTC 1 cut(s) 425
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 2 cut(s) 32, 89
AspS9I GGNCC 1 cut(s) 310
AsuC2I CCSGG 1 cut(s) 68
BbvI GCAGC 1 cut(s) 144
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 425
BisI GCNGC 3 cut(s) 133, 309, 382
BlsI GCNGC 3 cut(s) 134, 310, 383
Bme1390I CCNGG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 68
BpuMI CCSGG 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 449
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 115
BseNI ACTGG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 311
BsiSI CCGG 3 cut(s) 67, 278, 299
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 425
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 1 cut(s) 311
Bsp143I GATC 2 cut(s) 148, 241
BspACI CCGC 2 cut(s) 308, 381
BspANI GGCC 1 cut(s) 311
BspLI GGNNCC 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 391
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 2 cut(s) 148, 241
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 1 cut(s) 391
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 2 cut(s) 151, 244
BstMAI GTCTC 1 cut(s) 425
BstMBI GATC 2 cut(s) 148, 241
BstSCI CCNGG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 144
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 232
CviAII CATG 3 cut(s) 55, 137, 328
CviJI RGCY 8 cut(s) 29, 43, 135, 173, 289, 311, 357, 384
CviKI_1 RGCY 8 cut(s) 29, 43, 135, 173, 289, 311, 357, 384
CviQI GTAC 1 cut(s) 232
DpnI GATC 2 cut(s) 150, 243
DpnII GATC 2 cut(s) 148, 241
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
FaeI CATG 3 cut(s) 58, 140, 331
FaiI YATR 5 cut(s) 56, 138, 329, 351, 440
FatI CATG 3 cut(s) 54, 136, 327
Fnu4HI GCNGC 3 cut(s) 133, 309, 382
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 133, 309, 382
GluI GCNGC 3 cut(s) 133, 309, 382
HaeIII GGCC 1 cut(s) 311
HapII CCGG 3 cut(s) 67, 278, 299
Hin1II CATG 3 cut(s) 58, 140, 331
HinfI GANTC 1 cut(s) 295
HpaII CCGG 3 cut(s) 67, 278, 299
Hpy188I TCNGA 3 cut(s) 48, 246, 294
Hpy188III TCNNGA 1 cut(s) 418
HpyAV CCTTC 3 cut(s) 33, 85, 370
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 331
Hsp92II CATG 3 cut(s) 58, 140, 331
Kzo9I GATC 2 cut(s) 148, 241
LguI GCTCTTC 1 cut(s) 391
LpnPI CCDG 7 cut(s) 80, 123, 171, 291, 312, 326, 430
Lsp1109I GCAGC 1 cut(s) 144
MalI GATC 2 cut(s) 150, 243
MboI GATC 2 cut(s) 148, 241
MboII GAAGA 1 cut(s) 378
MluCI AATT 5 cut(s) 32, 89, 181, 248, 332
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 389
MnlI CCTC 3 cut(s) 19, 300, 405
MseI TTAA 2 cut(s) 180, 402
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 3 cut(s) 67, 278, 299
MspR9I CCNGG 1 cut(s) 68
Mva1269I GAATGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 148, 241
NlaIII CATG 3 cut(s) 58, 140, 331
NlaIV GGNNCC 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 391
PctI GAATGC 1 cut(s) 271
PkrI GCNGC 3 cut(s) 134, 310, 383
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 2 cut(s) 180, 402
SatI GCNGC 3 cut(s) 133, 309, 382
Sau3AI GATC 2 cut(s) 148, 241
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 68
SetI ASST 4 cut(s) 24, 31, 324, 416
Sse9I AATT 5 cut(s) 32, 89, 181, 248, 332
SsiI CCGC 2 cut(s) 308, 381
StyD4I CCNGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 160
TasI AATT 5 cut(s) 32, 89, 181, 248, 332
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 311, 384
Tru1I TTAA 2 cut(s) 180, 402
Tru9I TTAA 2 cut(s) 180, 402
TscAI CASTG 1 cut(s) 163
TseI GCWGC 1 cut(s) 132
TspRI CASTG 1 cut(s) 163
XapI RAATTY 2 cut(s) 32, 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.