Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005001

Physical Location & Seq

Genomic Coordinates Forward (+)

2500961 .. 2503063

Gene Structure 2103 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1020300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGCATTCATCTCGTCCATTTCTCTTCTGGGTCCTTTTCTTTTTCGTCTCCAACCTCAGTTTCCCTTCGCTTTCTGTCCCGTTCTTGCCTGCGACGAACAATAACCTCACCCTCTTCGGCGACGCTTCCATCACCAACAACGCGATTAGTCTCACTCAAGAACTCACCACCTGCCCCTCCTCTTCCCCTTCTGCTTCTTCTGGGGTTGGAAGAGCATTTTACGCCTACCCAATTCGGTTTCTTGACTCGAAAACCAACGCCACTGCCTCTTTCTTGTGCCGATTCTCTTTCTCAATCACCCCAAGTTCCCCATTGTGTTCTTCTGGAGATGGCATTGCGTTTGTGATCATCCCCGATGTCGATTCTCTGGGATCATCCAGAGGGGGGTATATGGGACTTCCGGAGCCATCCTCAGTGTCGAAAGAATCGTTCTTCGCTGTGGAATTCGATACTAGGTTTGATCCTATGGTTGGTGACATCAATGGGAACCACATTGGTGTTGATTTGAACACAGTTGTGTCTGTTGCCTGTGTTGATGTTGTTTCAAGAGGGATTGATTTGACAAGTGGGAGGGAGATTACTGCTTGGATTGAGTACAGGGATGCTGTCAAGATGATTCGGGTTTGGGTCGGATACTCGTCAACTAGGCCTCCAACGCCTGTTCTTGTTGCTCAGATTGACCTCTCCAACCAGTTCAAGGAGTTCATGCATGTCGGTTTCTCGGCCTCGAATGGGCAGGGCTCTTCAGTTCACGTTGTTGACAGGTGGAGATTCAAGACTTTCGGGTCTCTTCCACCTGGGAATCCTACAGATACCTTTGAAGAAGGGGACTGCTTCATGTGCTCTTCACCAGAAGATTCAAGTACCAAAAGCAGCCCGGAAAATCGTTCCCATGAAAGAAAAACAAAGATTGTGGAGATGGCTCTTGGGTTGGGAGGGTTAGCTGCATTTGTTGTCTCCATAATCGCATTTGTTTTTGTCATTTGTTTTGTGTGTGTGAAGAAGAGAATGGTCACTGCCAGAGGGAGCAGAGAGGGCAAAACTTGTAGAGTTGAGGCGAATAAAGTTCCGACGAGTTTGTCTCTTGCAGAGATAAGATCGGCGACAATGGGGTTTAACCGACACAGGGTTGTCGGGGAAGGGGCATCAGCTAAGGTTTATAAAGGGTCTCTGTCCTCTGGTGGAGAAGTGGCAGTGAAAAGGTTTGAGAGGGTTGATGGGATCGGTAGTCTGCGTAATCCTTTTACTACGGAGTTTGCAACTATGGTGGGTTGTTTGCGGCACAAGAATCTGATCCAGCTTCATGGGTGGTGCTGTGAGGGTAATGAGTTGGTCCTGGTGTACGAGTACATGCCCAACGGAAGCCTCAACAAAGTTCTTCACAAAAGCTTCAATTCAGCAGTTGTTCTGTCCTGGAAGCAAAGACTTAATATAGTTCTTGGAGTCGCTTCTGCTCTTGCATATCTTCACGAGGAGTGTGAGAGGCAAATAATTCACAGAGATGTCAAAACTTGCAACATAATGCTCGATGCAGATTTTAACGCCAAACTAGGGGATTTTGGTCTAGCAGAAGTGTATGAGCATAGTTCAATCATTGCAAGAGAAGCAACTATACCAGCTGGTACAATGGGATATCTTGCTCCGGAATACGTTTATTCAGGTGTTCCAACTGTTAAAACTGATGTTTACAGCTTTGGTGTGGTGGTGTTAGAGGTGGCAACGGGAAGAAAGCCGGTGGAGGATGATGGAACAGTGGTTGTTGATTGGGTTTGGAGCATGTGGGAAAAGGGGAAACTGATTGAAGCTGCTGATCTGAGGTTGATGGGGAACTTTGATATGGTAGAGATGGAGAGGATGCTGATGACTGGACTTGCTTGTGTGCACCCAAACCATGTGAAGAGGCCAACGGTGAAGGAAGCTGCAAGGATTCTTAAAGGCGAAACGCCTCTTCCTCTGCTGCCACCAAGAAAACCAAGGGTAAGCCTTCGCCCTGTTTATCCTGATGAATGTGAAGAAATCCCGCTTTGTGGTGGTGTTCGGCCAAGTCTTGAACTTGATGATGTACAATATTTGACTCCGAAGAGCCACTTTGGCAAGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016020 GO:0042742 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

701

Amino Acids

76.75

Weight (kDa)

6.46

Isoelectric Point (pI)

39.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	30 - 261	8.2e-56	Legume lectin domain
Pkinase	PF00069	372 - 585	1.4e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	374 - 639	1.3e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42120
fragaria_vesca	FvH4_4g18100 FvH4_4g18150 FvH4_4g18200 FvH4_5g32800 FvH4_5g32800
malus_domestica	MD08G1201700.v1.1 MD10G1019400.v1.1 MD10G1019500.v1.1 MD10G1019800.v1.1 MD10G1019900.v1.1 MD10G1020300.v1.1
prunus_persica	Prupe.8G022700_v2.0.a1
pyrus_communis	pycom10g01260
rosa_chinensis	RchiOBHm_Chr7g0230531
rosa_laevigata	RLG00000001488 RLG00000002646
rosa_multiflora	Rmu_co8495365.1_g000001 Rmu_sc0002799.1_g000003 Rmu_sc0005117.1_g000004
rosa_roxburghii	Rroxscaffold_3G00230050
rosa_rugosa	Rorug07G0261300
rosa_samantha	Rh7AG410200 Rh7AG412400 Rh7AG412500 Rh7BG389900 Rh7CG430100 Rh7CG430200 Rh7DG408000
rosa_wichuraiana	Rw7G034110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1161
AarI	CACCTGC	1 cut(s)	179
AasI	GACNNNNNNGTC	2 cut(s)	784, 1078
Acc36I	ACCTGC	1 cut(s)	179
AccII	CGCG	1 cut(s)	143
AccIII	TCCGGA	2 cut(s)	400, 1642
AciI	CCGC	2 cut(s)	1279, 2021
AclWI	GGATC	4 cut(s)	379, 455, 1229, 1288
AcoI	YGGCCR	1 cut(s)	2039
AcsI	RAATTY	1 cut(s)	443
AcuI	CTGAAG	1 cut(s)	729
AfaI	GTAC	6 cut(s)	596, 865, 1343, 1349, 1624, 2064
AfiI	CCNNNNNNNGG	7 cut(s)	384, 470, 697, 732, 1126, 1551, 2027
AjnI	CCWGG	3 cut(s)	796, 1335, 1412
AjuI	GAANNNNNNNTTGG	2 cut(s)	44, 76
AleI	CACNNNNGTG	2 cut(s)	495, 515
AluBI	AGCT	8 cut(s)	944, 1151, 1300, 1389, 1619, 1692, 1805, 1919
AluI	AGCT	8 cut(s)	944, 1151, 1300, 1389, 1619, 1692, 1805, 1919
Alw21I	GWGCWC	2 cut(s)	845, 1884
Alw26I	GTCTC	6 cut(s)	52, 155, 792, 961, 1086, 1173
Alw44I	GTGCAC	1 cut(s)	1880
AlwI	GGATC	4 cut(s)	379, 455, 1229, 1288
Aor13HI	TCCGGA	2 cut(s)	400, 1642
AoxI	GGCC	4 cut(s)	647, 723, 1901, 2039
ApaLI	GTGCAC	1 cut(s)	1880
ApeKI	GCWGC	5 cut(s)	873, 944, 1805, 1919, 1957
ApoI	RAATTY	1 cut(s)	443
AspS9I	GGNCC	2 cut(s)	31, 1333
AsuC2I	CCSGG	1 cut(s)	878
AsuHPI	GGTGA	7 cut(s)	100, 124, 157, 289, 485, 840, 1921
AvaII	GGWCC	2 cut(s)	31, 1333
BaeGI	GKGCMC	1 cut(s)	1884
BanII	GRGCYC	1 cut(s)	743
BarI	GAAGNNNNNNTAC	2 cut(s)	1596, 1628
BauI	CACGAG	1 cut(s)	1469
Bbv12I	GWGCWC	2 cut(s)	845, 1884
BbvI	GCAGC	5 cut(s)	885, 931, 1792, 1906, 1944
BccI	CCATC	8 cut(s)	137, 323, 415, 913, 1211, 1739, 1816, 1840
BcgI	CGANNNNNNTGC	1 cut(s)	27
BciT130I	CCWGG	3 cut(s)	798, 1337, 1414
BciVI	GTATCC	1 cut(s)	626
BclI	TGATCA	1 cut(s)	345
BcnI	CCSGG	1 cut(s)	878
BcoDI	GTCTC	6 cut(s)	52, 155, 792, 961, 1086, 1173
BfaI	CTAG	4 cut(s)	453, 645, 1550, 1565
BfmI	CTRYAG	1 cut(s)	807
BfuAI	ACCTGC	1 cut(s)	179
BfuI	GTATCC	1 cut(s)	626
BglI	GCCNNNNNGGC	1 cut(s)	2091
BisI	GCNGC	6 cut(s)	874, 945, 1280, 1806, 1920, 1958
BlsI	GCNGC	6 cut(s)	875, 946, 1281, 1807, 1921, 1959
Bme1390I	CCNGG	4 cut(s)	798, 878, 1337, 1414
Bme18I	GGWCC	2 cut(s)	31, 1333
BmgT120I	GGNCC	2 cut(s)	31, 1333
BmiI	GGNNCC	3 cut(s)	32, 405, 488
BmrFI	CCNGG	4 cut(s)	798, 878, 1337, 1414
BmsI	GCATC	4 cut(s)	592, 1154, 1519, 1845
BplI	GAGNNNNNCTC	4 cut(s)	395, 427, 1066, 1098
BpmI	CTGGAG	1 cut(s)	345
Bpu10I	CCTNAGC	1 cut(s)	1152
BpuEI	CTTGAG	1 cut(s)	141
BpuMI	CCSGG	1 cut(s)	878
BsaI	GGTCTC	2 cut(s)	792, 1173
BsaJI	CCNNGG	2 cut(s)	797, 1973
BsaWI	WCCGGW	2 cut(s)	400, 1642
BsaXI	ACNNNNNCTCC	2 cut(s)	634, 664
Bsc4I	CCNNNNNNNGG	7 cut(s)	384, 470, 697, 732, 1126, 1551, 2027
Bse118I	RCCGGY	1 cut(s)	1732
Bse1I	ACTGG	2 cut(s)	691, 1870
Bse3DI	GCAATG	2 cut(s)	333, 1593
BseAI	TCCGGA	2 cut(s)	400, 1642
BseBI	CCWGG	3 cut(s)	798, 1337, 1414
BseDI	CCNNGG	2 cut(s)	797, 1973
BseGI	GGATG	6 cut(s)	348, 374, 407, 607, 1747, 1860
BseLI	CCNNNNNNNGG	7 cut(s)	384, 470, 697, 732, 1126, 1551, 2027
BseMI	GCAATG	2 cut(s)	333, 1593
BseMII	CTCAG	4 cut(s)	70, 426, 686, 1805
BseNI	ACTGG	2 cut(s)	691, 1870
BseRI	GAGGAG	2 cut(s)	169, 1487
BseSI	GKGCMC	1 cut(s)	1884
BseXI	GCAGC	5 cut(s)	885, 931, 1792, 1906, 1944
Bsh1236I	CGCG	1 cut(s)	143
BshFI	GGCC	4 cut(s)	649, 725, 1903, 2041
BsiHKAI	GWGCWC	2 cut(s)	845, 1884
BsiSI	CCGG	4 cut(s)	401, 878, 1643, 1733
BslFI	GGGAC	3 cut(s)	62, 408, 842
BslI	CCNNNNNNNGG	7 cut(s)	384, 470, 697, 732, 1126, 1551, 2027
BsmAI	GTCTC	6 cut(s)	52, 155, 792, 961, 1086, 1173
BsmBI	CGTCTC	1 cut(s)	52
BsmFI	GGGAC	3 cut(s)	62, 408, 842
BsmI	GAATGC	1 cut(s)	4
BsnI	GGCC	4 cut(s)	649, 725, 1903, 2041
Bso31I	GGTCTC	2 cut(s)	792, 1173
Bsp1286I	GDGCHC	3 cut(s)	743, 845, 1884
Bsp13I	TCCGGA	2 cut(s)	400, 1642
Bsp1407I	TGTACA	1 cut(s)	2062
Bsp143I	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
BspACI	CCGC	2 cut(s)	1279, 2021
BspANI	GGCC	4 cut(s)	649, 725, 1903, 2041
BspCNI	CTCAG	4 cut(s)	69, 425, 685, 1806
BspEI	TCCGGA	2 cut(s)	400, 1642
BspFNI	CGCG	1 cut(s)	143
BspLI	GGNNCC	3 cut(s)	32, 405, 488
BspMI	ACCTGC	1 cut(s)	179
BspPI	GGATC	4 cut(s)	379, 455, 1229, 1288
BspQI	GCTCTTC	4 cut(s)	205, 748, 850, 2075
BspTNI	GGTCTC	2 cut(s)	792, 1173
BsrDI	GCAATG	2 cut(s)	333, 1593
BsrFI	RCCGGY	1 cut(s)	1732
BsrGI	TGTACA	1 cut(s)	2062
BsrI	ACTGG	2 cut(s)	691, 1870
BssAI	RCCGGY	1 cut(s)	1732
BssECI	CCNNGG	2 cut(s)	797, 1973
BssMI	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
BssSI	CACGAG	1 cut(s)	1469
BssT1I	CCWWGG	1 cut(s)	1973
Bst2BI	CACGAG	1 cut(s)	1469
Bst2UI	CCWGG	3 cut(s)	798, 1337, 1414
Bst4CI	ACNGT	4 cut(s)	514, 1672, 1753, 1909
BstAUI	TGTACA	1 cut(s)	2062
BstC8I	GCNNGC	1 cut(s)	90
BstDEI	CTNAG	5 cut(s)	56, 412, 672, 1152, 1814
BstF5I	GGATG	6 cut(s)	348, 374, 407, 607, 1747, 1860
BstFNI	CGCG	1 cut(s)	143
BstKTI	GATC	7 cut(s)	348, 374, 463, 1100, 1224, 1296, 1813
BstMAI	GTCTC	6 cut(s)	52, 155, 792, 961, 1086, 1173
BstMBI	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
BstMWI	GCNNNNNNNGC	6 cut(s)	221, 655, 840, 1035, 1604, 2091
BstNI	CCWGG	3 cut(s)	798, 1337, 1414
BstNSI	RCATGY	3 cut(s)	713, 1354, 1780
BstSCI	CCNGG	4 cut(s)	796, 876, 1335, 1412
BstSFI	CTRYAG	1 cut(s)	807
BstSLI	GKGCMC	1 cut(s)	1884
BstUI	CGCG	1 cut(s)	143
BstV1I	GCAGC	5 cut(s)	885, 931, 1792, 1906, 1944
BstXI	CCANNNNNNTGG	1 cut(s)	1304
BsuI	GTATCC	1 cut(s)	626
BsuRI	GGCC	4 cut(s)	649, 725, 1903, 2041
BtsCI	GGATG	6 cut(s)	348, 374, 407, 607, 1747, 1860
BtsI	GCAGTG	3 cut(s)	261, 1014, 1200
BtsIMutI	CAGTG	5 cut(s)	261, 420, 1014, 1200, 1758
BveI	ACCTGC	1 cut(s)	179
Cac8I	GCNNGC	1 cut(s)	90
Cfr10I	RCCGGY	1 cut(s)	1732
Cfr13I	GGNCC	2 cut(s)	31, 1333
CseI	GACGC	1 cut(s)	131
Csp6I	GTAC	6 cut(s)	595, 864, 1342, 1348, 1623, 2063
CspCI	CAANNNNNGTGG	4 cut(s)	894, 929, 1248, 1283
CviAII	CATG	8 cut(s)	706, 710, 838, 893, 1304, 1351, 1777, 1892
CviQI	GTAC	6 cut(s)	595, 864, 1342, 1348, 1623, 2063
DdeI	CTNAG	5 cut(s)	56, 412, 672, 1152, 1814
DpnI	GATC	7 cut(s)	347, 373, 462, 1099, 1223, 1295, 1812
DpnII	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
DrdI	GACNNNNNNGTC	2 cut(s)	784, 1078
DseDI	GACNNNNNNGTC	2 cut(s)	784, 1078
EaeI	YGGCCR	1 cut(s)	2039
Eco130I	CCWWGG	1 cut(s)	1973
Eco147I	AGGCCT	1 cut(s)	649
Eco24I	GRGCYC	1 cut(s)	743
Eco31I	GGTCTC	2 cut(s)	792, 1173
Eco32I	GATATC	1 cut(s)	1634
Eco47I	GGWCC	2 cut(s)	31, 1333
Eco57I	CTGAAG	1 cut(s)	729
EcoO109I	RGGNCCY	1 cut(s)	31
EcoRI	GAATTC	1 cut(s)	443
EcoRII	CCWGG	3 cut(s)	796, 1335, 1412
EcoRV	GATATC	1 cut(s)	1634
EcoT14I	CCWWGG	1 cut(s)	1973
EcoT22I	ATGCAT	2 cut(s)	6, 711
EcoT38I	GRGCYC	1 cut(s)	743
ErhI	CCWWGG	1 cut(s)	1973
Esp3I	CGTCTC	1 cut(s)	52
FaeI	CATG	8 cut(s)	709, 713, 841, 896, 1307, 1354, 1780, 1895
FalI	AAGNNNNNCTT	1 cut(s)	2072
FaqI	GGGAC	3 cut(s)	62, 408, 842
FatI	CATG	8 cut(s)	705, 709, 837, 892, 1303, 1350, 1776, 1891
FauI	CCCGC	1 cut(s)	2028
FbaI	TGATCA	1 cut(s)	345
Fnu4HI	GCNGC	6 cut(s)	874, 945, 1280, 1806, 1920, 1958
FokI	GGATG	6 cut(s)	335, 361, 394, 614, 1754, 1867
FriOI	GRGCYC	1 cut(s)	743
Fsp4HI	GCNGC	6 cut(s)	874, 945, 1280, 1806, 1920, 1958
FspBI	CTAG	4 cut(s)	453, 645, 1550, 1565
GluI	GCNGC	6 cut(s)	874, 945, 1280, 1806, 1920, 1958
GsuI	CTGGAG	1 cut(s)	345
HaeIII	GGCC	4 cut(s)	649, 725, 1903, 2041
HapII	CCGG	4 cut(s)	401, 878, 1643, 1733
HgaI	GACGC	1 cut(s)	131
Hin1II	CATG	8 cut(s)	709, 713, 841, 896, 1307, 1354, 1780, 1895
HincII	GTYRAC	2 cut(s)	642, 760
HindII	GTYRAC	2 cut(s)	642, 760
HindIII	AAGCTT	1 cut(s)	1387
HpaII	CCGG	4 cut(s)	401, 878, 1643, 1733
HphI	GGTGA	7 cut(s)	100, 124, 157, 289, 485, 840, 1921
Hpy166II	GTNNAC	6 cut(s)	642, 751, 760, 1342, 1687, 1882
Hpy188I	TCNGA	6 cut(s)	632, 675, 1071, 1293, 1815, 2079
Hpy8I	GTNNAC	6 cut(s)	642, 751, 760, 1342, 1687, 1882
Hpy99I	CGWCG	3 cut(s)	97, 125, 1075
HpyAV	CCTTC	6 cut(s)	75, 198, 818, 1133, 1906, 1994
HpyCH4III	ACNGT	4 cut(s)	514, 1672, 1753, 1909
HpyCH4IV	ACGT	2 cut(s)	753, 1650
HpyF10VI	GCNNNNNNNGC	6 cut(s)	221, 655, 840, 1035, 1604, 2091
HpyF3I	CTNAG	5 cut(s)	56, 412, 672, 1152, 1814
HpySE526I	ACGT	2 cut(s)	753, 1650
Hsp92II	CATG	8 cut(s)	709, 713, 841, 896, 1307, 1354, 1780, 1895
Kpn2I	TCCGGA	2 cut(s)	400, 1642
Ksp22I	TGATCA	1 cut(s)	345
Kzo9I	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
LguI	GCTCTTC	4 cut(s)	205, 748, 850, 2075
LmnI	GCTCC	4 cut(s)	403, 1026, 1645, 1773
Lsp1109I	GCAGC	5 cut(s)	885, 931, 1792, 1906, 1944
LweI	GCATC	4 cut(s)	592, 1154, 1519, 1845
MaeI	CTAG	4 cut(s)	453, 645, 1550, 1565
MaeII	ACGT	2 cut(s)	753, 1650
MaeIII	GTNAC	2 cut(s)	473, 1012
MalI	GATC	7 cut(s)	347, 373, 462, 1099, 1223, 1295, 1812
MboI	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
MhlI	GDGCHC	3 cut(s)	743, 845, 1884
MluCI	AATT	4 cut(s)	231, 443, 1393, 1491
MlyI	GAGTC	3 cut(s)	239, 1452, 2068
MmeI	TCCRAC	7 cut(s)	75, 187, 610, 677, 711, 1094, 1691
Mph1103I	ATGCAT	2 cut(s)	6, 711
MroI	TCCGGA	2 cut(s)	400, 1642
MseI	TTAA	5 cut(s)	1116, 1428, 1539, 1674, 1932
MslI	CAYNNNNRTG	3 cut(s)	495, 515, 1500
MspA1I	CMGCKG	1 cut(s)	1619
MspI	CCGG	4 cut(s)	401, 878, 1643, 1733
MspR9I	CCNGG	4 cut(s)	798, 878, 1337, 1414
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	3 cut(s)	798, 1337, 1414
MvnI	CGCG	1 cut(s)	143
MwoI	GCNNNNNNNGC	6 cut(s)	221, 655, 840, 1035, 1604, 2091
NciI	CCSGG	1 cut(s)	878
NdeII	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
NlaIII	CATG	8 cut(s)	709, 713, 841, 896, 1307, 1354, 1780, 1895
NlaIV	GGNNCC	3 cut(s)	32, 405, 488
NmeAIII	GCCGAG	1 cut(s)	701
NmuCI	GTSAC	2 cut(s)	473, 1012
NsiI	ATGCAT	2 cut(s)	6, 711
NspI	RCATGY	3 cut(s)	713, 1354, 1780
OliI	CACNNNNGTG	2 cut(s)	495, 515
PaqCI	CACCTGC	1 cut(s)	179
PceI	AGGCCT	1 cut(s)	649
PciSI	GCTCTTC	4 cut(s)	205, 748, 850, 2075
PcsI	WCGNNNNNNNCGW	1 cut(s)	425
PctI	GAATGC	1 cut(s)	4
PfeI	GAWTC	9 cut(s)	282, 362, 425, 616, 771, 802, 857, 1288, 1927
PfoI	TCCNGGA	1 cut(s)	1412
PkrI	GCNGC	6 cut(s)	875, 946, 1281, 1807, 1921, 1959
PleI	GAGTC	3 cut(s)	239, 1451, 2068
PpsI	GAGTC	3 cut(s)	239, 1451, 2068
PpuMI	RGGWCCY	1 cut(s)	31
PsiI	TTATAA	1 cut(s)	1161
Psp5II	RGGWCCY	1 cut(s)	31
Psp6I	CCWGG	3 cut(s)	796, 1335, 1412
PspGI	CCWGG	3 cut(s)	796, 1335, 1412
PspN4I	GGNNCC	3 cut(s)	32, 405, 488
PspPI	GGNCC	2 cut(s)	31, 1333
PspPPI	RGGWCCY	1 cut(s)	31
PvuII	CAGCTG	1 cut(s)	1619
RsaI	GTAC	6 cut(s)	596, 865, 1343, 1349, 1624, 2064
RsaNI	GTAC	6 cut(s)	595, 864, 1342, 1348, 1623, 2063
RseI	CAYNNNNRTG	3 cut(s)	495, 515, 1500
SapI	GCTCTTC	4 cut(s)	205, 748, 850, 2075
SaqAI	TTAA	5 cut(s)	1116, 1428, 1539, 1674, 1932
SatI	GCNGC	6 cut(s)	874, 945, 1280, 1806, 1920, 1958
Sau3AI	GATC	7 cut(s)	345, 371, 460, 1097, 1221, 1293, 1810
Sau96I	GGNCC	2 cut(s)	31, 1333
SchI	GAGTC	3 cut(s)	239, 1452, 2068
ScrFI	CCNGG	4 cut(s)	798, 878, 1337, 1414
SduI	GDGCHC	3 cut(s)	743, 845, 1884
SfaNI	GCATC	4 cut(s)	592, 1154, 1519, 1845
SfcI	CTRYAG	1 cut(s)	807
SinI	GGWCC	2 cut(s)	31, 1333
SmiMI	CAYNNNNRTG	3 cut(s)	495, 515, 1500
SmlI	CTYRAG	1 cut(s)	156
SmoI	CTYRAG	1 cut(s)	156
Sse9I	AATT	4 cut(s)	231, 443, 1393, 1491
SseBI	AGGCCT	1 cut(s)	649
SsiI	CCGC	2 cut(s)	1279, 2021
SspI	AATATT	1 cut(s)	2069
SspMI	CTAG	4 cut(s)	453, 645, 1550, 1565
StuI	AGGCCT	1 cut(s)	649
StyD4I	CCNGG	4 cut(s)	796, 876, 1335, 1412
StyI	CCWWGG	1 cut(s)	1973
TaaI	ACNGT	4 cut(s)	514, 1672, 1753, 1909
TaiI	ACGT	2 cut(s)	756, 1653
TaqI	TCGA	6 cut(s)	248, 360, 419, 447, 728, 1527
TasI	AATT	4 cut(s)	231, 443, 1393, 1491
TatI	WGTACW	3 cut(s)	594, 1347, 2062
TauI	GCSGC	1 cut(s)	1282
TfiI	GAWTC	9 cut(s)	282, 362, 425, 616, 771, 802, 857, 1288, 1927
Tru1I	TTAA	5 cut(s)	1116, 1428, 1539, 1674, 1932
Tru9I	TTAA	5 cut(s)	1116, 1428, 1539, 1674, 1932
TscAI	CASTG	5 cut(s)	268, 420, 1021, 1200, 1758
TseFI	GTSAC	2 cut(s)	473, 1012
TseI	GCWGC	5 cut(s)	873, 944, 1805, 1919, 1957
Tsp45I	GTSAC	2 cut(s)	473, 1012
TspDTI	ATGAA	5 cut(s)	694, 826, 909, 1292, 2019
TspGWI	ACGGA	2 cut(s)	1265, 1374
TspRI	CASTG	5 cut(s)	268, 420, 1021, 1200, 1758
VneI	GTGCAC	1 cut(s)	1880
VpaK11BI	GGWCC	2 cut(s)	31, 1333
XapI	RAATTY	1 cut(s)	443
XceI	RCATGY	3 cut(s)	713, 1354, 1780
XcmI	CCANNNNNNNNNTGG	1 cut(s)	24
XspI	CTAG	4 cut(s)	453, 645, 1550, 1565
Zsp2I	ATGCAT	2 cut(s)	6, 711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.