Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003721

Physical Location & Seq

Genomic Coordinates Reverse (-)

22563188 .. 22568986

Gene Structure 5799 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g18710.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGAGGGAGAGGATTCAGGACACGCTCGCCGCTCACCGGAACGAACTTGTTTCTCTTCTCTCCAGGTATGTTGATCAAGGAAATGGGATACTGCAGCCTCATCAGATAATCAACGAGCTCGATAAAGTGATCGAAGAGGATGAGAAGATGAAGAAGCTCAAGGATAGCCCCTTCAGCAAAGTTCTACAGTCTGCACAGGAAGCAATTGTTCTGCCTCCTTTTGTGACTCTTGCACTTCGTCCAAGGCCTGGTGTATGGGAATATGTCCGTGTCAATGTCCATGAACTCAGTGTGGATCATTTGAGTGTTGCCGAGTATCTCGGGTTCAAAGAAGAACTTCTAGATGGAGAGTACAATGACAATTATTTGCTTGAACTTGATTTGGAGCCATTTAATTCAACATTCCCTCGGCCAACCCGTTCATCATCCATTGGGAATGGGGTTCAATTCCTTAACCGTCACTTATCTTCAATTATGTTCCGTAACAAAGATAGTTTGGAGCCTCTTCTTGATTTCCTTCGTACACATAAACATGATGGCCATGCAATGATGCTGAACGATCGAATTCAGAGCATACCCAAGCTTCAGTCTGCTCTAGCCAAGGCAGAGGAATATGTTTCTAAGTTCCCACCAATGACACCATACTCTGAGTTCGAGTTCGAGTTACAAGGAATGGGATTTGAGAGAGGGTGGGGTGACACTGCAGGACGTGTGTCGGAGATGGTGCATCTTCTCTTGGAAATTCTGCAAGCTCCAGATCCTTCTACACTTGAAACATTTCTTGGCAGGATTCCTATGGTGTTCAATGTTGTTATCGTGTCTCCACATGGCTACTTTGGCCAAGCAAATGTATTGGGTTTGCCGGACACTGGTGGGCAGGTTGTTTATATACTGGACCAAGTGCGCGCCCTGGAGAATGAGATGCTTCTTAGAATAAAGAAGCAAGGACTGGATATTATTCCAAAAATTCTTATTGTTACTCGACTGCTACCTGATGCAAAAGGAACAACATGCAACCAGAGATTAGAAAGAGTTAGTGGTACAGAAAACACATATATCTTGCGTGTACCTTTCAGGACAAAGAATGGAATTCTGAGGAAATGGATTTCAAGGTTTGATGTTTGGCCTTACTTGGAGACCTTTACAGAGGATGCGTCAAATGAAATTGCTGCTGAGCTACAGGGTGTTCCAGATCTGATCATTGGAAACTACAGTGATGGAAATCTTGTTGCAACTTTGTTATCCAATAAGCTAGGAATCACACAGTGCAATATTGCTCATGCATTAGAGAAGACGAAGTACCCAGATTCTGATATATTTTGGAAACGCTACGAGGACAAGTACCATTTCTCAAGTCAATTCACTGCCGATCTCATTGCAATGAACAGTGCAGATTTCATAATCACCAGTACATACCAAGAGATTGCGGGAAGCCAGAACAATGTTGGACAGTATGAGAGCCACACAGCTTTCACTCTTCCTGGGCTGTATCGAGTTGTTCATGGGGTTGATGTTTTTGATACTAAGTTTAATATTGTCTCTCCAGGAGCAGATATGTGCATATATTTTCCATATTCTGACAGCGAAAACAGACTTACAGCACTGCATGGTTCAATTGAAGAACTTATATATGGGGCTGAGCAGAATGATAAGCATATTGGGTTCTTGGCTGATCGATCAAAGCCTATGGTGTTTTCCATGGCAAGACTTGATAGAGTGAAAAATTTAACTGGACTAGTTGAGTGCTATGGTAAGAGCACCAAACTAAGAGAAATGGTGAATCTTGTTGTGGTTGGTGGTTACATGGATGCCAAGAACTCTAGGGATAGAGAAGAAATGGTAGAGATTGAAAAGATGCACGACCTCATTAAGAAGTACGACTTGCATGGCCAGTTTAGATGGATAGCGGCACAAATGAACCGTGCGCGTAATGGTGAGCTGTACCGCTACATTGCAGACACAAGAGGTGTCTTTGTGCAGCCTGCTTTCTATGAAGCTTTTGGCCTCACTGTTGTGGAAGCCATGACTTGTGGCCTTCCTACTTTTGCCACTTGTCATGGTGGACCTGCTGAGATCATTGAGCATGGTGTCTCAGGGTTCCATATTGATCCCTATCACCCTGATCAAGTAGCAGAAACTCTGATCGACTTCTTTGGTCAGTGCCAAAAGGATCCTAGCCACTGGGAAAAGATATCTGGAGCTGGGCTTAAACGAATTTATGGAAGGTACACATGGAAAATATATTCTGAGAGGCTGCTCACCTTAGCTGGGGTTTATGGTTTCTGGAAGCACGTTTCTAAGCTTGAGAGGCGAGAAGCAAGGCGATATCTGGAGATGTTTTACACTCTCAAGTACCGAAGTCTGGTGAAGAACATTCTGCTGGCTGTTGATGAGCATCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0006073 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009311 GO:0009505 GO:0009628 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016020 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0035251 GO:0036293 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070482 GO:0071695 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

801

Amino Acids

91.33

Weight (kDa)

5.94

Isoelectric Point (pI)

37.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	1 - 111	1.4e-47	Sucrose synthase N-terminal
SUS_EPBD	PF24862	146 - 233	8.2e-35	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	256 - 544	8.1e-166	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	557 - 718	3.9e-28	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	562 - 716	1.6e-14	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49190 AT5G49190 AT5G49190
fragaria_vesca	FvH4_4g18710
malus_domestica	MD13G1164000.v1.1 MD13G1164100.v1.1 MD13G1164200.v1.1 MD16G1164000.v1.1
prunus_persica	Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1
pyrus_communis	pycom13g14010 pycom16g13860 pycom16g13870
rosa_chinensis	RchiOBHm_Chr4g0423411
rosa_laevigata	RLG00000007559
rosa_multiflora	Rmu_sc0000893.1_g000010 Rmu_ssc0000174.1_g000013
rosa_roxburghii	Rroxscaffold_4G00291020 Rroxscaffold_5G00365630
rosa_rugosa	Rorug04G0187400 Rorug04G0187500 Rorug04G0187600 Rorug04G0187700 Rorug07G0218200
rosa_samantha	Rh3BG353800 Rh4AG246400 Rh4BG251800 Rh4CG260300 Rh4DG247200 Rh6BG052100
rosa_wichuraiana	Rw1G026460 Rw4G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	868, 2074
AccB7I	CCANNNNNTGG	1 cut(s)	248
AccBSI	CCGCTC	1 cut(s)	32
AccII	CGCG	2 cut(s)	906, 1927
AciI	CCGC	4 cut(s)	30, 1427, 1907, 1945
AclWI	GGATC	5 cut(s)	303, 752, 2102, 2165, 2178
AcoI	YGGCCR	4 cut(s)	410, 538, 838, 1888
AcsI	RAATTY	6 cut(s)	564, 741, 966, 1089, 1723, 2214
AcuI	CTGAAG	2 cut(s)	157, 569
AdeI	CACNNNGTG	1 cut(s)	1266
AfiI	CCNNNNNNNGG	5 cut(s)	36, 248, 869, 2268, 2362
AflIII	ACRYGT	1 cut(s)	709
AhlI	ACTAGT	1 cut(s)	1735
AjiI	CACGTC	1 cut(s)	710
AjnI	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
AjuI	GAANNNNNNNTTGG	2 cut(s)	765, 797
AleI	CACNNNNGTG	1 cut(s)	2012
Alw21I	GWGCWC	2 cut(s)	120, 1760
Alw26I	GTCTC	4 cut(s)	825, 1130, 1543, 2095
AlwI	GGATC	5 cut(s)	303, 752, 2102, 2165, 2178
AlwNI	CAGNNNCTG	1 cut(s)	1310
Ama87I	CYCGRG	1 cut(s)	320
AoxI	GGCC	8 cut(s)	245, 410, 538, 838, 1124, 1888, 2002, 2032
ApeKI	GCWGC	4 cut(s)	94, 1169, 1978, 2254
ApoI	RAATTY	6 cut(s)	564, 741, 966, 1089, 1723, 2214
Asp700I	GAANNNNTTC	2 cut(s)	336, 777
AspLEI	GCGC	3 cut(s)	906, 908, 1927
AspS9I	GGNCC	2 cut(s)	895, 2063
AsuHPI	GGTGA	8 cut(s)	26, 707, 1396, 1789, 1946, 2108, 2251, 2377
AvaI	CYCGRG	1 cut(s)	320
AvaII	GGWCC	2 cut(s)	895, 2063
BalI	TGGCCA	3 cut(s)	540, 840, 1890
BamHI	GGATCC	1 cut(s)	2170
BanII	GRGCYC	1 cut(s)	120
BarI	GAAGNNNNNNTAC	2 cut(s)	1283, 1315
BbsI	GAAGAC	1 cut(s)	1298
Bbv12I	GWGCWC	2 cut(s)	120, 1760
BbvI	GCAGC	4 cut(s)	106, 1156, 1990, 2241
BccI	CCATC	5 cut(s)	338, 530, 715, 1211, 1893
BcgI	CGANNNNNNTGC	2 cut(s)	1358, 1392
BciT130I	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
BciVI	GTATCC	1 cut(s)	81
BclI	TGATCA	3 cut(s)	73, 1197, 2122
BcoDI	GTCTC	4 cut(s)	825, 1130, 1543, 2095
BcuI	ACTAGT	1 cut(s)	1735
BfaI	CTAG	6 cut(s)	341, 596, 1253, 1736, 1821, 2175
BfmI	CTRYAG	5 cut(s)	92, 185, 702, 1178, 1210
BfuAI	ACCTGC	2 cut(s)	868, 2074
BfuI	GTATCC	1 cut(s)	81
BglII	AGATCT	1 cut(s)	1192
BisI	GCNGC	6 cut(s)	30, 95, 1170, 1908, 1979, 2255
BlpI	GCTNAGC	2 cut(s)	1173, 1638
BlsI	GCNGC	6 cut(s)	31, 96, 1171, 1909, 1980, 2256
Bme1390I	CCNGG	5 cut(s)	64, 249, 911, 1482, 1545
Bme18I	GGWCC	2 cut(s)	895, 2063
BmeT110I	CYCGRG	1 cut(s)	320
BmgBI	CACGTC	1 cut(s)	710
BmgT120I	GGNCC	2 cut(s)	895, 2063
BmiI	GGNNCC	4 cut(s)	387, 501, 2099, 2172
BmrFI	CCNGG	5 cut(s)	64, 249, 911, 1482, 1545
BmrI	ACTGGG	1 cut(s)	2191
BmsI	GCATC	7 cut(s)	540, 736, 912, 985, 1141, 1798, 1845
BmuI	ACTGGG	1 cut(s)	2191
BoxI	GACNNNNGTC	1 cut(s)	712
BpiI	GAAGAC	1 cut(s)	1298
BpmI	CTGGAG	6 cut(s)	46, 738, 932, 1527, 2217, 2351
Bpu10I	CCTNAGC	1 cut(s)	2263
Bpu1102I	GCTNAGC	2 cut(s)	1173, 1638
BpuEI	CTTGAG	4 cut(s)	143, 1336, 2324, 2333
Bsa29I	ATCGAT	1 cut(s)	1675
BsaBI	GATNNNNATC	3 cut(s)	1221, 2112, 2394
BsaI	GGTCTC	1 cut(s)	1130
BsaJI	CCNNGG	6 cut(s)	242, 407, 600, 909, 1481, 1698
BsaWI	WCCGGW	1 cut(s)	36
Bsc4I	CCNNNNNNNGG	5 cut(s)	36, 248, 869, 2268, 2362
Bse1I	ACTGG	7 cut(s)	874, 897, 954, 1407, 1735, 1891, 2186
Bse3DI	GCAATG	4 cut(s)	552, 1374, 1386, 1950
Bse8I	GATNNNNATC	3 cut(s)	1221, 2112, 2394
BseBI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
BseCI	ATCGAT	1 cut(s)	1675
BseDI	CCNNGG	6 cut(s)	242, 407, 600, 909, 1481, 1698
BseGI	GGATG	4 cut(s)	145, 425, 1156, 1813
BseJI	GATNNNNATC	3 cut(s)	1221, 2112, 2394
BseLI	CCNNNNNNNGG	5 cut(s)	36, 248, 869, 2268, 2362
BseMI	GCAATG	4 cut(s)	552, 1374, 1386, 1950
BseMII	CTCAG	8 cut(s)	301, 639, 1085, 1164, 1629, 2061, 2106, 2238
BseNI	ACTGG	7 cut(s)	874, 897, 954, 1407, 1735, 1891, 2186
BsePI	GCGCGC	1 cut(s)	904
BseXI	GCAGC	4 cut(s)	106, 1156, 1990, 2241
BseYI	CCCAGC	2 cut(s)	2201, 2267
BsgI	GTGCAG	3 cut(s)	177, 1410, 1997
Bsh1236I	CGCG	2 cut(s)	906, 1927
Bsh1285I	CGRYCG	1 cut(s)	562
BshFI	GGCC	8 cut(s)	247, 412, 540, 840, 1126, 1890, 2004, 2034
BshVI	ATCGAT	1 cut(s)	1675
BsiEI	CGRYCG	1 cut(s)	562
BsiHKAI	GWGCWC	2 cut(s)	120, 1760
BsiHKCI	CYCGRG	1 cut(s)	320
BsiSI	CCGG	2 cut(s)	37, 863
BslI	CCNNNNNNNGG	5 cut(s)	36, 248, 869, 2268, 2362
BsmAI	GTCTC	4 cut(s)	825, 1130, 1543, 2095
BsnI	GGCC	8 cut(s)	247, 412, 540, 840, 1126, 1890, 2004, 2034
Bso31I	GGTCTC	1 cut(s)	1130
BsoBI	CYCGRG	1 cut(s)	320
Bsp1286I	GDGCHC	2 cut(s)	120, 1760
Bsp1720I	GCTNAGC	2 cut(s)	1173, 1638
Bsp19I	CCATGG	1 cut(s)	1698
BspACI	CCGC	4 cut(s)	30, 1427, 1907, 1945
BspANI	GGCC	8 cut(s)	247, 412, 540, 840, 1126, 1890, 2004, 2034
BspCNI	CTCAG	8 cut(s)	300, 640, 1086, 1165, 1630, 2062, 2105, 2239
BspDI	ATCGAT	1 cut(s)	1675
BspFNI	CGCG	2 cut(s)	906, 1927
BspLI	GGNNCC	4 cut(s)	387, 501, 2099, 2172
BspMAI	CTGCAG	2 cut(s)	96, 706
BspMI	ACCTGC	2 cut(s)	868, 2074
BspPI	GGATC	5 cut(s)	303, 752, 2102, 2165, 2178
BspTNI	GGTCTC	1 cut(s)	1130
BsrBI	CCGCTC	1 cut(s)	32
BsrDI	GCAATG	4 cut(s)	552, 1374, 1386, 1950
BsrI	ACTGG	7 cut(s)	874, 897, 954, 1407, 1735, 1891, 2186
BssECI	CCNNGG	6 cut(s)	242, 407, 600, 909, 1481, 1698
BssHII	GCGCGC	1 cut(s)	904
BssT1I	CCWWGG	3 cut(s)	242, 600, 1698
Bst2UI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
Bst4CI	ACNGT	8 cut(s)	189, 458, 1214, 1266, 1388, 1452, 1922, 2011
Bst6I	CTCTTC	4 cut(s)	60, 129, 510, 1482
BstC8I	GCNNGC	5 cut(s)	27, 750, 906, 1983, 2382
BstDSI	CCRYGG	1 cut(s)	1698
BstF5I	GGATG	4 cut(s)	145, 425, 1156, 1813
BstFNI	CGCG	2 cut(s)	906, 1927
BstHHI	GCGC	3 cut(s)	906, 908, 1927
BstMAI	GTCTC	4 cut(s)	825, 1130, 1543, 2095
BstMCI	CGRYCG	1 cut(s)	562
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 200, 837, 2308
BstNI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
BstNSI	RCATGY	1 cut(s)	1014
BstPAI	GACNNNNGTC	1 cut(s)	712
BstSCI	CCNGG	5 cut(s)	62, 247, 909, 1480, 1543
BstSFI	CTRYAG	5 cut(s)	92, 185, 702, 1178, 1210
BstUI	CGCG	2 cut(s)	906, 1927
BstV1I	GCAGC	4 cut(s)	106, 1156, 1990, 2241
BstV2I	GAAGAC	1 cut(s)	1298
BstX2I	RGATCY	3 cut(s)	757, 1192, 2170
BstYI	RGATCY	3 cut(s)	757, 1192, 2170
Bsu15I	ATCGAT	1 cut(s)	1675
BsuI	GTATCC	1 cut(s)	81
BsuRI	GGCC	8 cut(s)	247, 412, 540, 840, 1126, 1890, 2004, 2034
BsuTUI	ATCGAT	1 cut(s)	1675
BtgI	CCRYGG	1 cut(s)	1698
BtrI	CACGTC	1 cut(s)	710
BtsCI	GGATG	4 cut(s)	145, 425, 1156, 1813
BtsI	GCAGTG	3 cut(s)	699, 1362, 1601
BveI	ACCTGC	2 cut(s)	868, 2074
Cac8I	GCNNGC	5 cut(s)	27, 750, 906, 1983, 2382
CaiI	CAGNNNCTG	1 cut(s)	1310
CfoI	GCGC	3 cut(s)	906, 908, 1927
Cfr13I	GGNCC	2 cut(s)	895, 2063
ClaI	ATCGAT	1 cut(s)	1675
CseI	GACGC	1 cut(s)	1143
DraIII	CACNNNGTG	1 cut(s)	1266
EaeI	YGGCCR	4 cut(s)	410, 538, 838, 1888
Eam1104I	CTCTTC	4 cut(s)	60, 129, 510, 1482
EarI	CTCTTC	4 cut(s)	60, 129, 510, 1482
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	3 cut(s)	242, 600, 1698
Eco147I	AGGCCT	1 cut(s)	247
Eco24I	GRGCYC	1 cut(s)	120
Eco31I	GGTCTC	1 cut(s)	1130
Eco32I	GATATC	2 cut(s)	2193, 2327
Eco47I	GGWCC	2 cut(s)	895, 2063
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	2 cut(s)	157, 569
Eco88I	CYCGRG	1 cut(s)	320
EcoICRI	GAGCTC	1 cut(s)	118
EcoRI	GAATTC	2 cut(s)	564, 1089
EcoRII	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
EcoRV	GATATC	2 cut(s)	2193, 2327
EcoT14I	CCWWGG	3 cut(s)	242, 600, 1698
EcoT22I	ATGCAT	1 cut(s)	1285
EcoT38I	GRGCYC	1 cut(s)	120
ErhI	CCWWGG	3 cut(s)	242, 600, 1698
FalI	AAGNNNNNCTT	4 cut(s)	321, 353, 1865, 1897
FauI	CCCGC	1 cut(s)	1420
FbaI	TGATCA	3 cut(s)	73, 1197, 2122
Fnu4HI	GCNGC	6 cut(s)	30, 95, 1170, 1908, 1979, 2255
FokI	GGATG	4 cut(s)	152, 412, 1163, 1820
FriOI	GRGCYC	1 cut(s)	120
Fsp4HI	GCNGC	6 cut(s)	30, 95, 1170, 1908, 1979, 2255
FspBI	CTAG	6 cut(s)	341, 596, 1253, 1736, 1821, 2175
GlaI	GCGC	3 cut(s)	905, 907, 1926
GluI	GCNGC	6 cut(s)	30, 95, 1170, 1908, 1979, 2255
GsaI	CCCAGC	2 cut(s)	2205, 2271
GsuI	CTGGAG	6 cut(s)	46, 738, 932, 1527, 2217, 2351
HaeIII	GGCC	8 cut(s)	247, 412, 540, 840, 1126, 1890, 2004, 2034
HapII	CCGG	2 cut(s)	37, 863
HgaI	GACGC	1 cut(s)	1143
HhaI	GCGC	3 cut(s)	906, 908, 1927
Hin6I	GCGC	3 cut(s)	904, 906, 1925
HinP1I	GCGC	3 cut(s)	904, 906, 1925
HindIII	AAGCTT	3 cut(s)	581, 1995, 2300
HinfI	GANTC	6 cut(s)	13, 226, 790, 1257, 1307, 1780
HpaII	CCGG	2 cut(s)	37, 863
HphI	GGTGA	8 cut(s)	26, 707, 1396, 1789, 1946, 2108, 2251, 2377
Hpy166II	GTNNAC	4 cut(s)	524, 1067, 2063, 2229
Hpy188III	TCNNGA	9 cut(s)	17, 341, 509, 755, 1075, 1190, 2196, 2284, 2330
Hpy8I	GTNNAC	4 cut(s)	524, 1067, 2063, 2229
HpyAV	CCTTC	5 cut(s)	181, 527, 771, 2045, 2217
HpyCH4III	ACNGT	8 cut(s)	189, 458, 1214, 1266, 1388, 1452, 1922, 2011
HpyCH4IV	ACGT	2 cut(s)	709, 2292
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 200, 837, 2308
HpySE526I	ACGT	2 cut(s)	709, 2292
HspAI	GCGC	3 cut(s)	904, 906, 1925
Ksp22I	TGATCA	3 cut(s)	73, 1197, 2122
LmnI	GCTCC	5 cut(s)	385, 499, 757, 1547, 2198
Lsp1109I	GCAGC	4 cut(s)	106, 1156, 1990, 2241
LweI	GCATC	7 cut(s)	540, 736, 912, 985, 1141, 1798, 1845
MaeI	CTAG	6 cut(s)	341, 596, 1253, 1736, 1821, 2175
MaeII	ACGT	2 cut(s)	709, 2292
MaeIII	GTNAC	7 cut(s)	223, 458, 482, 663, 695, 976, 1799
MbiI	CCGCTC	1 cut(s)	32
MfeI	CAATTG	2 cut(s)	204, 1614
MflI	RGATCY	3 cut(s)	757, 1192, 2170
MhlI	GDGCHC	2 cut(s)	120, 1760
MlsI	TGGCCA	3 cut(s)	540, 840, 1890
MluNI	TGGCCA	3 cut(s)	540, 840, 1890
MlyI	GAGTC	1 cut(s)	220
MmeI	TCCRAC	2 cut(s)	696, 1426
Mox20I	TGGCCA	3 cut(s)	540, 840, 1890
Mph1103I	ATGCAT	1 cut(s)	1285
MroXI	GAANNNNTTC	2 cut(s)	336, 777
MscI	TGGCCA	3 cut(s)	540, 840, 1890
MseI	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
MslI	CAYNNNNRTG	4 cut(s)	303, 531, 1379, 2012
Msp20I	TGGCCA	3 cut(s)	540, 840, 1890
MspI	CCGG	2 cut(s)	37, 863
MspR9I	CCNGG	5 cut(s)	64, 249, 911, 1482, 1545
MunI	CAATTG	2 cut(s)	204, 1614
MvaI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
MvnI	CGCG	2 cut(s)	906, 1927
MwoI	GCNNNNNNNGC	4 cut(s)	174, 200, 837, 2308
NcoI	CCATGG	1 cut(s)	1698
NlaIV	GGNNCC	4 cut(s)	387, 501, 2099, 2172
NmeAIII	GCCGAG	2 cut(s)	337, 388
NmuCI	GTSAC	3 cut(s)	223, 458, 695
NsiI	ATGCAT	1 cut(s)	1285
NspI	RCATGY	1 cut(s)	1014
OliI	CACNNNNGTG	1 cut(s)	2012
PauI	GCGCGC	1 cut(s)	904
PceI	AGGCCT	1 cut(s)	247
PcsI	WCGNNNNNNNCGW	1 cut(s)	415
PdmI	GAANNNNTTC	2 cut(s)	336, 777
PfeI	GAWTC	5 cut(s)	13, 790, 1257, 1307, 1780
PflMI	CCANNNNNTGG	1 cut(s)	248
PfoI	TCCNGGA	1 cut(s)	1543
PkrI	GCNGC	6 cut(s)	31, 96, 1171, 1909, 1980, 2256
Ple19I	CGATCG	1 cut(s)	562
PleI	GAGTC	1 cut(s)	220
PpsI	GAGTC	1 cut(s)	220
PshAI	GACNNNNGTC	1 cut(s)	712
Psp124BI	GAGCTC	1 cut(s)	120
Psp6I	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
PspFI	CCCAGC	2 cut(s)	2201, 2267
PspGI	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
PspN4I	GGNNCC	4 cut(s)	387, 501, 2099, 2172
PspPI	GGNCC	2 cut(s)	895, 2063
PsrI	GAACNNNNNNTAC	2 cut(s)	308, 340
PstI	CTGCAG	2 cut(s)	96, 706
PstNI	CAGNNNCTG	1 cut(s)	1310
PsuI	RGATCY	3 cut(s)	757, 1192, 2170
PteI	GCGCGC	1 cut(s)	904
PvuI	CGATCG	1 cut(s)	562
RseI	CAYNNNNRTG	4 cut(s)	303, 531, 1379, 2012
SacI	GAGCTC	1 cut(s)	120
SaqAI	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
SatI	GCNGC	6 cut(s)	30, 95, 1170, 1908, 1979, 2255
Sau96I	GGNCC	2 cut(s)	895, 2063
SchI	GAGTC	1 cut(s)	220
ScrFI	CCNGG	5 cut(s)	64, 249, 911, 1482, 1545
SduI	GDGCHC	2 cut(s)	120, 1760
SfaNI	GCATC	7 cut(s)	540, 736, 912, 985, 1141, 1798, 1845
SfcI	CTRYAG	5 cut(s)	92, 185, 702, 1178, 1210
SinI	GGWCC	2 cut(s)	895, 2063
SmiMI	CAYNNNNRTG	4 cut(s)	303, 531, 1379, 2012
SmlI	CTYRAG	4 cut(s)	158, 1351, 2303, 2348
SmoI	CTYRAG	4 cut(s)	158, 1351, 2303, 2348
SpeI	ACTAGT	1 cut(s)	1735
SseBI	AGGCCT	1 cut(s)	247
SsiI	CCGC	4 cut(s)	30, 1427, 1907, 1945
SspI	AATATT	2 cut(s)	1273, 1534
SspMI	CTAG	6 cut(s)	341, 596, 1253, 1736, 1821, 2175
SstI	GAGCTC	1 cut(s)	120
StuI	AGGCCT	1 cut(s)	247
StyD4I	CCNGG	5 cut(s)	62, 247, 909, 1480, 1543
StyI	CCWWGG	3 cut(s)	242, 600, 1698
TaaI	ACNGT	8 cut(s)	189, 458, 1214, 1266, 1388, 1452, 1922, 2011
TaiI	ACGT	2 cut(s)	712, 2295
TaqI	TCGA	9 cut(s)	120, 132, 562, 654, 660, 982, 1492, 1675, 2145
TatI	WGTACW	2 cut(s)	351, 1409
TauI	GCSGC	2 cut(s)	32, 1910
TfiI	GAWTC	5 cut(s)	13, 790, 1257, 1307, 1780
Tru1I	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
Tru9I	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
TseFI	GTSAC	3 cut(s)	223, 458, 695
TseI	GCWGC	4 cut(s)	94, 1169, 1978, 2254
Tsp45I	GTSAC	3 cut(s)	223, 458, 695
TspDTI	ATGAA	9 cut(s)	164, 297, 411, 1176, 1387, 1397, 1490, 1931, 2007
TspGWI	ACGGA	2 cut(s)	257, 470
Van91I	CCANNNNNTGG	1 cut(s)	248
VpaK11BI	GGWCC	2 cut(s)	895, 2063
XapI	RAATTY	6 cut(s)	564, 741, 966, 1089, 1723, 2214
XbaI	TCTAGA	1 cut(s)	340
XceI	RCATGY	1 cut(s)	1014
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1442
XmnI	GAANNNNTTC	2 cut(s)	336, 777
XspI	CTAG	6 cut(s)	341, 596, 1253, 1736, 1821, 2175
Zsp2I	ATGCAT	1 cut(s)	1285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.