Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003721

Physical Location & Seq

Genomic Coordinates Forward (+)

18185124 .. 18190312

Gene Structure 5189 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007559

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGCGGGAGAGGATCCAGGACACGCTCGCCGCACACCGGAACGAACTTGTTTCTCTTCTCTCCAGGTATGTTGCTCGAGGAAATGGGATACTACAGCCTCATCAGATAATCAACGAGCTCGAAAAGGTGATCGAAGAGGATGAGAAGATGAAGAAGCTAAAGGATAGCCCCTTCAGCAAAGTTCTACAGTCTGCACAGGAAGCAATTGTTCTGCCTCCTTTTGTGGCTTTTGCTCTTCGTCCAAGACCTGGTGTATGGGAATATGTCCGTGTCAATGTATATGAACTCAGTGTGGATCATCTGAGTGTTGCAGAATATCTCCTGTTCAAAGAAGAACTTCTGGATGGAGAGTGCAATGACAATTATGTGCTTGAACTTGACTTGGAGCCATTTAATGCAACATTCCCTCGGCCAACCCGTTCATCATCCATTGGGAATGGGGTTCAATTCCTTAACCGTCACTTGTCTTCAATTATGTTCCGTAACAAAGAAAGTTTGGAGCCTCTTCTTGATTTCCTTCGTACGCATAAACATGATGGCCATGCAATGATGTTGAACGATCGAATTCAGAGCATACCCAAGCTTCAGTCTGCTTTAGCCAAGGCAGAGGAATATCTTTCTAAGTTCCCCCCAACGACACCATATTCTGAGTTTGAGTTTGAGTATGCAATTCATCTATTTTCAAATAATTTGATGTCCGGTAACTTAAAGAGGCGACTTGATTTCTTTAGACATTACCTTTATCCTCTTCACTCTAAGTGTAGGTTACAAGGAATGGGATTTGAGAGAGGGTGGGGTGACACTGCAGGACAGGTGTCAGAGATGGTGCATCTACTCTTGGAAATTCTTCAGGCTCCAGATCCTTCTACACTTGAAACATTTCTTGGCAGGATTCCTATGGTGTTCAATGTTGTCATTGTGTCTCCACACGGCTACTTTGGCCAAGCAAATGTATTGGGTTTGCCGGACACGGGTGGGCAGGTTGTTTATATACTGGATCAAGTGCGGGCCCTGGAGAATGAGATGCTTCTTAGAATACAGAAGCAAGGACTGGATGTTATTCCAAAGATTCTGATTGTTACTCGACTGCTACCTGATGCAAAAGGAACAACGTGCAACCAGAGATTGGAAAGAGTTAGTGGTACAGAAAACACATATATCTTGCGGGTACCCTTCAGGACTAAGAATGGAATTCTCCGGAAATGGATTTCAAGGTTTGATGTCTGGCCTTACTTGGAGACCTTTGCAGAGGATGCATCAAACGAAATTGCCGCTGAGCTACAGGGTGTTCCAGATCTGATCATTGGAAACTACAGTGATGGAAATCTTGTTGCAACTTTGTTATCTTATAAACTAGGAATCACACAGTGCAACATTGCTCATGCATTGGAGAAGACAAAGTACCCAGATTCTGATATATTTTGGAAAAGCTACGAGGACAAGTACCATTTCTCAAGTCAATTCACCGCCGATCTCATTGCAATGAACAATGCAGATTTCATAATCACCAGTACATACCAAGAGATTGCGGGAAGCCAGAACAATGTTGGACAGTATGAGAGCCACACAGCTTTCACTCTTCCTGGGCTGTATCGAGTTGTTCATGGGGTTGATGTTTTTGATCCTAAGTTTAATATTGTGTCTCCAGGAGCAGATATGTGCATATATTTTTCATATTCTGACAGGGAAAAAAGACTTACAGCACTGCATGGCACAATTGAAGAACTTATGTATGGGGCTGAGCAGAATGATGAGCATATTGGGTTCTTGGCTGATCGATCAAAGCCTACTGTGTTTTCCATGGCAAGACTTGATAGAGTGAAAAATTTAACTGGACTCGTTGAGTGCTATGGTAAGAGTACCAAACTAAGAGAAATGCCTGCTTTCTACGAAGCTTTTGGCCTCACTGTTGTGGAAGCCATGACTTGTGGCGTTCCTACTTTTGCCACTTGTCATGGTGGCCCTGCTGAGATCATTGAGCATGGTGTCTCAGGTTTCCATATTGATCCCTATCACCCTGATCAAGTGGCTGAAAGTCTGATCGACTTCTTTGGTCAGTGCCAAAAGGATCCTAGCCACTGGGAAAAGATATCTGGAGCCGGGCTTAAACGAATTTATGAAAGGTACACGTGGAAGATATATTCTGAGAGGCTGCTCACATTAGCTGGGGTTTATGGCTTCTGGAAGCACGTGTCGAAGCTTGAGAGGCGAGAGGCAAGGCGATATCTGGAAATGTTTTACATTCTCAAGTACCGAAGTCTGGTGAAGAACATTCCGCTGGCTGTTGATGAGCAGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0006073 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009311 GO:0009505 GO:0009628 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016020 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0035251 GO:0036293 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070482 GO:0071695 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

767

Amino Acids

87.53

Weight (kDa)

6.01

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	1 - 111	1e-47	Sucrose synthase N-terminal
SUS_EPBD	PF24862	146 - 221	1.5e-26	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	290 - 578	8.9e-166	Sucrose synthase first GT-B domain
Glyco_trans_1_4	PF13692	625 - 682	7.4e-11	Glycosyl transferases group 1
Glycos_transf_1	PF00534	626 - 684	9.4e-16	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49190 AT5G49190 AT5G49190
fragaria_vesca	FvH4_4g18710
malus_domestica	MD13G1164000.v1.1 MD13G1164100.v1.1 MD13G1164200.v1.1 MD16G1164000.v1.1
prunus_persica	Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1
pyrus_communis	pycom13g14010 pycom16g13860 pycom16g13870
rosa_chinensis	RchiOBHm_Chr4g0423411
rosa_laevigata	RLG00000007559
rosa_multiflora	Rmu_sc0000893.1_g000010 Rmu_ssc0000174.1_g000013
rosa_roxburghii	Rroxscaffold_4G00291020 Rroxscaffold_5G00365630
rosa_rugosa	Rorug04G0187400 Rorug04G0187500 Rorug04G0187600 Rorug04G0187700 Rorug07G0218200
rosa_samantha	Rh3BG353800 Rh4AG246400 Rh4BG251800 Rh4CG260300 Rh4DG247200 Rh6BG052100
rosa_wichuraiana	Rw1G026460 Rw4G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1350
Acc36I	ACCTGC	1 cut(s)	970
Acc65I	GGTACC	1 cut(s)	1168
AccB1I	GGYRCC	1 cut(s)	1168
AccB7I	CCANNNNNTGG	2 cut(s)	248, 1126
AccIII	TCCGGA	1 cut(s)	1197
AciI	CCGC	8 cut(s)	4, 30, 1006, 1165, 1272, 1467, 1529, 2276
AclWI	GGATC	9 cut(s)	7, 20, 303, 854, 1005, 1616, 2000, 2063, 2076
AcoI	YGGCCR	3 cut(s)	410, 538, 940
AcsI	RAATTY	5 cut(s)	564, 843, 1191, 1825, 2112
AcuI	CTGAAG	4 cut(s)	157, 569, 833, 1159
AcvI	CACGTG	2 cut(s)	2130, 2191
AdeI	CACNNNGTG	1 cut(s)	1368
AfaI	GTAC	9 cut(s)	523, 1144, 1170, 1403, 1445, 1513, 1861, 2126, 2252
AfiI	CCNNNNNNNGG	5 cut(s)	36, 248, 971, 1126, 2260
AflIII	ACRYGT	2 cut(s)	2127, 2190
AjnI	CCWGG	6 cut(s)	15, 62, 247, 1011, 1582, 1645
AjuI	GAANNNNNNNTTGG	2 cut(s)	867, 899
AleI	CACNNNNGTG	1 cut(s)	1910
AluBI	AGCT	9 cut(s)	118, 157, 583, 1279, 1431, 1571, 1895, 2165, 2200
AluI	AGCT	9 cut(s)	118, 157, 583, 1279, 1431, 1571, 1895, 2165, 2200
Alw21I	GWGCWC	1 cut(s)	120
Alw26I	GTCTC	4 cut(s)	927, 1232, 1647, 1993
AlwI	GGATC	9 cut(s)	7, 20, 303, 854, 1005, 1616, 2000, 2063, 2076
AlwNI	CAGNNNCTG	1 cut(s)	1412
Ama87I	CYCGRG	1 cut(s)	75
Aor13HI	TCCGGA	1 cut(s)	1197
AoxI	GGCC	7 cut(s)	410, 538, 940, 1008, 1226, 1900, 1960
ApaI	GGGCCC	1 cut(s)	1012
ApeKI	GCWGC	2 cut(s)	2152, 2293
ApoI	RAATTY	5 cut(s)	564, 843, 1191, 1825, 2112
Asp700I	GAANNNNTTC	3 cut(s)	336, 846, 879
Asp718I	GGTACC	1 cut(s)	1168
AspS9I	GGNCC	3 cut(s)	1008, 1009, 1961
AsuC2I	CCSGG	1 cut(s)	2101
AsuHPI	GGTGA	6 cut(s)	139, 809, 1456, 1498, 2006, 2275
AvaI	CYCGRG	1 cut(s)	75
BaeGI	GKGCMC	1 cut(s)	1012
BalI	TGGCCA	2 cut(s)	540, 942
BamHI	GGATCC	2 cut(s)	12, 2068
BanI	GGYRCC	1 cut(s)	1168
BanII	GRGCYC	2 cut(s)	120, 1012
BarI	GAAGNNNNNNTAC	2 cut(s)	1385, 1417
BbrPI	CACGTG	2 cut(s)	2130, 2191
BbsI	GAAGAC	2 cut(s)	459, 1400
Bbv12I	GWGCWC	1 cut(s)	120
BbvI	GCAGC	1 cut(s)	2139
BccI	CCATC	4 cut(s)	338, 530, 817, 1313
BceAI	ACGGC	1 cut(s)	946
BcgI	CGANNNNNNTGC	2 cut(s)	1460, 1494
BciT130I	CCWGG	6 cut(s)	17, 64, 249, 1013, 1584, 1647
BciVI	GTATCC	1 cut(s)	81
BclI	TGATCA	2 cut(s)	1299, 2020
BcnI	CCSGG	1 cut(s)	2101
BcoDI	GTCTC	4 cut(s)	927, 1232, 1647, 1993
BfaI	CTAG	2 cut(s)	1355, 2073
BfmI	CTRYAG	5 cut(s)	92, 185, 804, 1280, 1312
BfuAI	ACCTGC	1 cut(s)	970
BfuI	GTATCC	1 cut(s)	81
BglII	AGATCT	1 cut(s)	1294
BisI	GCNGC	4 cut(s)	30, 1272, 2153, 2294
BlpI	GCTNAGC	2 cut(s)	1275, 1740
BlsI	GCNGC	4 cut(s)	31, 1273, 2154, 2295
Bme1390I	CCNGG	7 cut(s)	17, 64, 249, 1013, 1584, 1647, 2101
BmeT110I	CYCGRG	1 cut(s)	75
BmgT120I	GGNCC	3 cut(s)	1008, 1009, 1961
BmiI	GGNNCC	8 cut(s)	14, 387, 501, 855, 1010, 1170, 2070, 2098
BmrFI	CCNGG	7 cut(s)	17, 64, 249, 1013, 1584, 1647, 2101
BmrI	ACTGGG	1 cut(s)	2089
BmsI	GCATC	5 cut(s)	838, 1014, 1087, 1243, 1265
BmuI	ACTGGG	1 cut(s)	2089
BoxI	GACNNNNGTC	1 cut(s)	814
BpiI	GAAGAC	2 cut(s)	459, 1400
BpmI	CTGGAG	5 cut(s)	46, 840, 1034, 1629, 2115
Bpu1102I	GCTNAGC	2 cut(s)	1275, 1740
BpuEI	CTTGAG	3 cut(s)	1438, 2222, 2231
BpuMI	CCSGG	1 cut(s)	2101
Bsa29I	ATCGAT	1 cut(s)	1777
BsaAI	YACGTR	2 cut(s)	2130, 2191
BsaBI	GATNNNNATC	2 cut(s)	1323, 2010
BsaI	GGTCTC	1 cut(s)	1232
BsaJI	CCNNGG	5 cut(s)	407, 600, 1011, 1583, 1800
BsaWI	WCCGGW	3 cut(s)	36, 698, 1197
Bsc4I	CCNNNNNNNGG	5 cut(s)	36, 248, 971, 1126, 2260
Bse1I	ACTGG	5 cut(s)	999, 1056, 1509, 1837, 2084
Bse3DI	GCAATG	5 cut(s)	361, 552, 1374, 1476, 1488
Bse8I	GATNNNNATC	2 cut(s)	1323, 2010
BseAI	TCCGGA	1 cut(s)	1197
BseBI	CCWGG	6 cut(s)	17, 64, 249, 1013, 1584, 1647
BseCI	ATCGAT	1 cut(s)	1777
BseDI	CCNNGG	5 cut(s)	407, 600, 1011, 1583, 1800
BseGI	GGATG	5 cut(s)	145, 349, 425, 1060, 1258
BseJI	GATNNNNATC	2 cut(s)	1323, 2010
BseLI	CCNNNNNNNGG	5 cut(s)	36, 248, 971, 1126, 2260
BseMI	GCAATG	5 cut(s)	361, 552, 1374, 1476, 1488
BseMII	CTCAG	8 cut(s)	293, 301, 639, 1266, 1731, 1959, 2004, 2136
BseNI	ACTGG	5 cut(s)	999, 1056, 1509, 1837, 2084
BseSI	GKGCMC	1 cut(s)	1012
BseXI	GCAGC	1 cut(s)	2139
BseYI	CCCAGC	1 cut(s)	2165
BsgI	GTGCAG	1 cut(s)	177
Bsh1285I	CGRYCG	1 cut(s)	562
BshFI	GGCC	7 cut(s)	412, 540, 942, 1010, 1228, 1902, 1962
BshNI	GGYRCC	1 cut(s)	1168
BshVI	ATCGAT	1 cut(s)	1777
BsiEI	CGRYCG	1 cut(s)	562
BsiHKAI	GWGCWC	1 cut(s)	120
BsiHKCI	CYCGRG	1 cut(s)	75
BsiSI	CCGG	5 cut(s)	37, 699, 965, 1198, 2100
BsiWI	CGTACG	1 cut(s)	521
BslI	CCNNNNNNNGG	5 cut(s)	36, 248, 971, 1126, 2260
BsmAI	GTCTC	4 cut(s)	927, 1232, 1647, 1993
BsnI	GGCC	7 cut(s)	412, 540, 942, 1010, 1228, 1902, 1962
Bso31I	GGTCTC	1 cut(s)	1232
BsoBI	CYCGRG	1 cut(s)	75
Bsp120I	GGGCCC	1 cut(s)	1008
Bsp1286I	GDGCHC	2 cut(s)	120, 1012
Bsp13I	TCCGGA	1 cut(s)	1197
Bsp1720I	GCTNAGC	2 cut(s)	1275, 1740
Bsp19I	CCATGG	1 cut(s)	1800
BspACI	CCGC	8 cut(s)	4, 30, 1006, 1165, 1272, 1467, 1529, 2276
BspANI	GGCC	7 cut(s)	412, 540, 942, 1010, 1228, 1902, 1962
BspCNI	CTCAG	8 cut(s)	294, 300, 640, 1267, 1732, 1960, 2003, 2137
BspDI	ATCGAT	1 cut(s)	1777
BspEI	TCCGGA	1 cut(s)	1197
BspLI	GGNNCC	8 cut(s)	14, 387, 501, 855, 1010, 1170, 2070, 2098
BspMAI	CTGCAG	1 cut(s)	808
BspMI	ACCTGC	1 cut(s)	970
BspPI	GGATC	9 cut(s)	7, 20, 303, 854, 1005, 1616, 2000, 2063, 2076
BspQI	GCTCTTC	1 cut(s)	240
BspT107I	GGYRCC	1 cut(s)	1168
BspTNI	GGTCTC	1 cut(s)	1232
BsrDI	GCAATG	5 cut(s)	361, 552, 1374, 1476, 1488
BsrI	ACTGG	5 cut(s)	999, 1056, 1509, 1837, 2084
BssECI	CCNNGG	5 cut(s)	407, 600, 1011, 1583, 1800
BssT1I	CCWWGG	2 cut(s)	600, 1800
Bst2UI	CCWGG	6 cut(s)	17, 64, 249, 1013, 1584, 1647
Bst4CI	ACNGT	7 cut(s)	189, 458, 1316, 1368, 1554, 1792, 1909
Bst6I	CTCTTC	6 cut(s)	60, 129, 240, 510, 753, 1584
BstAPI	GCANNNNNTGC	1 cut(s)	1253
BstBAI	YACGTR	2 cut(s)	2130, 2191
BstC8I	GCNNGC	4 cut(s)	27, 1008, 1881, 2280
BstDSI	CCRYGG	1 cut(s)	1800
BstF5I	GGATG	5 cut(s)	145, 349, 425, 1060, 1258
BstMAI	GTCTC	4 cut(s)	927, 1232, 1647, 1993
BstMCI	CGRYCG	1 cut(s)	562
BstMWI	GCNNNNNNNGC	5 cut(s)	174, 200, 939, 1253, 2206
BstNI	CCWGG	6 cut(s)	17, 64, 249, 1013, 1584, 1647
BstPAI	GACNNNNGTC	1 cut(s)	814
BstSCI	CCNGG	7 cut(s)	15, 62, 247, 1011, 1582, 1645, 2099
BstSFI	CTRYAG	5 cut(s)	92, 185, 804, 1280, 1312
BstSLI	GKGCMC	1 cut(s)	1012
BstV1I	GCAGC	1 cut(s)	2139
BstV2I	GAAGAC	2 cut(s)	459, 1400
BstX2I	RGATCY	4 cut(s)	12, 859, 1294, 2068
BstYI	RGATCY	4 cut(s)	12, 859, 1294, 2068
Bsu15I	ATCGAT	1 cut(s)	1777
BsuI	GTATCC	1 cut(s)	81
BsuRI	GGCC	7 cut(s)	412, 540, 942, 1010, 1228, 1902, 1962
BsuTUI	ATCGAT	1 cut(s)	1777
BtgI	CCRYGG	1 cut(s)	1800
BtsCI	GGATG	5 cut(s)	145, 349, 425, 1060, 1258
BtsI	GCAGTG	2 cut(s)	801, 1703
BtsIMutI	CAGTG	9 cut(s)	295, 801, 1321, 1373, 1703, 1905, 2063, 2077, 2296
BveI	ACCTGC	1 cut(s)	970
Cac8I	GCNNGC	4 cut(s)	27, 1008, 1881, 2280
CaiI	CAGNNNCTG	1 cut(s)	1412
Cfr13I	GGNCC	3 cut(s)	1008, 1009, 1961
ClaI	ATCGAT	1 cut(s)	1777
CsiI	ACCWGGT	1 cut(s)	247
Csp6I	GTAC	9 cut(s)	522, 1143, 1169, 1402, 1444, 1512, 1860, 2125, 2251
CviAII	CATG	9 cut(s)	533, 542, 1382, 1604, 1709, 1801, 1921, 1955, 1982
CviQI	GTAC	9 cut(s)	522, 1143, 1169, 1402, 1444, 1512, 1860, 2125, 2251
DraIII	CACNNNGTG	1 cut(s)	1368
EaeI	YGGCCR	3 cut(s)	410, 538, 940
Eam1104I	CTCTTC	6 cut(s)	60, 129, 240, 510, 753, 1584
EarI	CTCTTC	6 cut(s)	60, 129, 240, 510, 753, 1584
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	2 cut(s)	600, 1800
Eco24I	GRGCYC	2 cut(s)	120, 1012
Eco31I	GGTCTC	1 cut(s)	1232
Eco32I	GATATC	2 cut(s)	2091, 2225
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	4 cut(s)	157, 569, 833, 1159
Eco72I	CACGTG	2 cut(s)	2130, 2191
Eco88I	CYCGRG	1 cut(s)	75
EcoICRI	GAGCTC	1 cut(s)	118
EcoO109I	RGGNCCY	1 cut(s)	1009
EcoRI	GAATTC	2 cut(s)	564, 1191
EcoRII	CCWGG	6 cut(s)	15, 62, 247, 1011, 1582, 1645
EcoRV	GATATC	2 cut(s)	2091, 2225
EcoT14I	CCWWGG	2 cut(s)	600, 1800
EcoT22I	ATGCAT	2 cut(s)	1258, 1387
EcoT38I	GRGCYC	2 cut(s)	120, 1012
ErhI	CCWWGG	2 cut(s)	600, 1800
FaeI	CATG	9 cut(s)	536, 545, 1385, 1607, 1712, 1804, 1924, 1958, 1985
FalI	AAGNNNNNCTT	2 cut(s)	321, 353
FatI	CATG	9 cut(s)	532, 541, 1381, 1603, 1708, 1800, 1920, 1954, 1981
FauI	CCCGC	3 cut(s)	999, 1158, 1522
FbaI	TGATCA	2 cut(s)	1299, 2020
Fnu4HI	GCNGC	4 cut(s)	30, 1272, 2153, 2294
FokI	GGATG	5 cut(s)	152, 356, 412, 1067, 1265
FriOI	GRGCYC	2 cut(s)	120, 1012
Fsp4HI	GCNGC	4 cut(s)	30, 1272, 2153, 2294
FspBI	CTAG	2 cut(s)	1355, 2073
GluI	GCNGC	4 cut(s)	30, 1272, 2153, 2294
GsaI	CCCAGC	1 cut(s)	2169
GsuI	CTGGAG	5 cut(s)	46, 840, 1034, 1629, 2115
HaeIII	GGCC	7 cut(s)	412, 540, 942, 1010, 1228, 1902, 1962
HapII	CCGG	5 cut(s)	37, 699, 965, 1198, 2100
Hin1II	CATG	9 cut(s)	536, 545, 1385, 1607, 1712, 1804, 1924, 1958, 1985
HindIII	AAGCTT	3 cut(s)	581, 1893, 2198
HinfI	GANTC	5 cut(s)	892, 1069, 1359, 1409, 1836
HpaII	CCGG	5 cut(s)	37, 699, 965, 1198, 2100
HphI	GGTGA	6 cut(s)	139, 809, 1456, 1498, 2006, 2275
Hpy166II	GTNNAC	1 cut(s)	2127
Hpy188III	TCNNGA	9 cut(s)	341, 509, 857, 1177, 1198, 1292, 2094, 2182, 2228
Hpy8I	GTNNAC	1 cut(s)	2127
HpyAV	CCTTC	4 cut(s)	181, 527, 873, 1183
HpyCH4III	ACNGT	7 cut(s)	189, 458, 1316, 1368, 1554, 1792, 1909
HpyCH4IV	ACGT	3 cut(s)	1112, 2129, 2190
HpyF10VI	GCNNNNNNNGC	5 cut(s)	174, 200, 939, 1253, 2206
HpySE526I	ACGT	3 cut(s)	1112, 2129, 2190
Hsp92II	CATG	9 cut(s)	536, 545, 1385, 1607, 1712, 1804, 1924, 1958, 1985
Kpn2I	TCCGGA	1 cut(s)	1197
KpnI	GGTACC	1 cut(s)	1172
Ksp22I	TGATCA	2 cut(s)	1299, 2020
LguI	GCTCTTC	1 cut(s)	240
LmnI	GCTCC	5 cut(s)	385, 499, 859, 1649, 2096
Lsp1109I	GCAGC	1 cut(s)	2139
LweI	GCATC	5 cut(s)	838, 1014, 1087, 1243, 1265
MabI	ACCWGGT	1 cut(s)	247
MaeI	CTAG	2 cut(s)	1355, 2073
MaeII	ACGT	3 cut(s)	1112, 2129, 2190
MaeIII	GTNAC	6 cut(s)	458, 482, 701, 765, 797, 1078
MfeI	CAATTG	2 cut(s)	204, 1716
MflI	RGATCY	4 cut(s)	12, 859, 1294, 2068
MhlI	GDGCHC	2 cut(s)	120, 1012
MlsI	TGGCCA	2 cut(s)	540, 942
MluNI	TGGCCA	2 cut(s)	540, 942
MlyI	GAGTC	1 cut(s)	1830
MmeI	TCCRAC	1 cut(s)	1528
Mox20I	TGGCCA	2 cut(s)	540, 942
Mph1103I	ATGCAT	2 cut(s)	1258, 1387
MroI	TCCGGA	1 cut(s)	1197
MroXI	GAANNNNTTC	3 cut(s)	336, 846, 879
MscI	TGGCCA	2 cut(s)	540, 942
MseI	TTAA	6 cut(s)	393, 453, 707, 1632, 1829, 2106
MslI	CAYNNNNRTG	4 cut(s)	303, 531, 1481, 1910
Msp20I	TGGCCA	2 cut(s)	540, 942
MspA1I	CMGCKG	2 cut(s)	1274, 2278
MspI	CCGG	5 cut(s)	37, 699, 965, 1198, 2100
MspR9I	CCNGG	7 cut(s)	17, 64, 249, 1013, 1584, 1647, 2101
MunI	CAATTG	2 cut(s)	204, 1716
MvaI	CCWGG	6 cut(s)	17, 64, 249, 1013, 1584, 1647
MwoI	GCNNNNNNNGC	5 cut(s)	174, 200, 939, 1253, 2206
NciI	CCSGG	1 cut(s)	2101
NcoI	CCATGG	1 cut(s)	1800
NlaIII	CATG	9 cut(s)	536, 545, 1385, 1607, 1712, 1804, 1924, 1958, 1985
NlaIV	GGNNCC	8 cut(s)	14, 387, 501, 855, 1010, 1170, 2070, 2098
NmeAIII	GCCGAG	1 cut(s)	388
NmuCI	GTSAC	2 cut(s)	458, 797
NsiI	ATGCAT	2 cut(s)	1258, 1387
OliI	CACNNNNGTG	1 cut(s)	1910
PaeR7I	CTCGAG	1 cut(s)	75
PciSI	GCTCTTC	1 cut(s)	240
PcsI	WCGNNNNNNNCGW	1 cut(s)	415
PdmI	GAANNNNTTC	3 cut(s)	336, 846, 879
PfeI	GAWTC	4 cut(s)	892, 1069, 1359, 1409
Pfl23II	CGTACG	1 cut(s)	521
PflMI	CCANNNNNTGG	2 cut(s)	248, 1126
PfoI	TCCNGGA	2 cut(s)	15, 1645
PkrI	GCNGC	4 cut(s)	31, 1273, 2154, 2295
Ple19I	CGATCG	1 cut(s)	562
PleI	GAGTC	1 cut(s)	1830
PmaCI	CACGTG	2 cut(s)	2130, 2191
PmlI	CACGTG	2 cut(s)	2130, 2191
PpsI	GAGTC	1 cut(s)	1830
Ppu21I	YACGTR	2 cut(s)	2130, 2191
PshAI	GACNNNNGTC	1 cut(s)	814
PsiI	TTATAA	1 cut(s)	1350
Psp124BI	GAGCTC	1 cut(s)	120
Psp6I	CCWGG	6 cut(s)	15, 62, 247, 1011, 1582, 1645
PspCI	CACGTG	2 cut(s)	2130, 2191
PspFI	CCCAGC	1 cut(s)	2165
PspGI	CCWGG	6 cut(s)	15, 62, 247, 1011, 1582, 1645
PspLI	CGTACG	1 cut(s)	521
PspN4I	GGNNCC	8 cut(s)	14, 387, 501, 855, 1010, 1170, 2070, 2098
PspOMI	GGGCCC	1 cut(s)	1008
PspPI	GGNCC	3 cut(s)	1008, 1009, 1961
PspXI	VCTCGAGB	1 cut(s)	75
PstI	CTGCAG	1 cut(s)	808
PstNI	CAGNNNCTG	1 cut(s)	1412
PsuI	RGATCY	4 cut(s)	12, 859, 1294, 2068
PvuI	CGATCG	1 cut(s)	562
RsaI	GTAC	9 cut(s)	523, 1144, 1170, 1403, 1445, 1513, 1861, 2126, 2252
RsaNI	GTAC	9 cut(s)	522, 1143, 1169, 1402, 1444, 1512, 1860, 2125, 2251
RseI	CAYNNNNRTG	4 cut(s)	303, 531, 1481, 1910
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	240
SaqAI	TTAA	6 cut(s)	393, 453, 707, 1632, 1829, 2106
SatI	GCNGC	4 cut(s)	30, 1272, 2153, 2294
Sau96I	GGNCC	3 cut(s)	1008, 1009, 1961
SchI	GAGTC	1 cut(s)	1830
ScrFI	CCNGG	7 cut(s)	17, 64, 249, 1013, 1584, 1647, 2101
SduI	GDGCHC	2 cut(s)	120, 1012
SexAI	ACCWGGT	1 cut(s)	247
SfaNI	GCATC	5 cut(s)	838, 1014, 1087, 1243, 1265
SfcI	CTRYAG	5 cut(s)	92, 185, 804, 1280, 1312
Sfr274I	CTCGAG	1 cut(s)	75
SlaI	CTCGAG	1 cut(s)	75
SmiMI	CAYNNNNRTG	4 cut(s)	303, 531, 1481, 1910
SmlI	CTYRAG	4 cut(s)	75, 1453, 2201, 2246
SmoI	CTYRAG	4 cut(s)	75, 1453, 2201, 2246
SsiI	CCGC	8 cut(s)	4, 30, 1006, 1165, 1272, 1467, 1529, 2276
SspI	AATATT	1 cut(s)	1636
SspMI	CTAG	2 cut(s)	1355, 2073
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	7 cut(s)	15, 62, 247, 1011, 1582, 1645, 2099
StyI	CCWWGG	2 cut(s)	600, 1800
TaaI	ACNGT	7 cut(s)	189, 458, 1316, 1368, 1554, 1792, 1909
TaiI	ACGT	3 cut(s)	1115, 2132, 2193
TaqI	TCGA	9 cut(s)	76, 120, 132, 562, 1084, 1594, 1777, 2043, 2195
TatI	WGTACW	1 cut(s)	1511
TauI	GCSGC	2 cut(s)	32, 1274
TfiI	GAWTC	4 cut(s)	892, 1069, 1359, 1409
Tru1I	TTAA	6 cut(s)	393, 453, 707, 1632, 1829, 2106
Tru9I	TTAA	6 cut(s)	393, 453, 707, 1632, 1829, 2106
TscAI	CASTG	8 cut(s)	295, 808, 1321, 1373, 1710, 1912, 2063, 2084
TseFI	GTSAC	2 cut(s)	458, 797
TseI	GCWGC	2 cut(s)	2152, 2293
Tsp45I	GTSAC	2 cut(s)	458, 797
TspDTI	ATGAA	9 cut(s)	164, 297, 411, 662, 1489, 1499, 1592, 1662, 2133
TspGWI	ACGGA	2 cut(s)	257, 470
TspRI	CASTG	8 cut(s)	295, 808, 1321, 1373, 1710, 1912, 2063, 2084
Van91I	CCANNNNNTGG	2 cut(s)	248, 1126
XapI	RAATTY	5 cut(s)	564, 843, 1191, 1825, 2112
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1544
XhoI	CTCGAG	1 cut(s)	75
XmnI	GAANNNNTTC	3 cut(s)	336, 846, 879
XspI	CTAG	2 cut(s)	1355, 2073
Zsp2I	ATGCAT	2 cut(s)	1258, 1387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.