Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0003721

Physical Location & Seq

Genomic Coordinates Reverse (-)

32532082 .. 32598256

Gene Structure 66175 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0187500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3858 bp

ATGGTGATCATTCAAGAAGCCTCTTCTTCTTCTTCTTCTTCTTCTTACTCCACCCATCCATGGAAATACCATGTCTTTTTGAGTTTTAGAGGTGAGGATACACGCAATAATTTCACAGGCCATTTGTGCGACGCTTTGCGTCAGAATGGAATCAACACCTTCATGGATGATCAGCTCAGACGAGGAGAAGAAATATCAACAGCACTTCTCCAAGCAATTGAACAGTCGAGGATTTCAATCATTGTGTTCTCTAAAAACTATGCATCCTCCAAGTGGTGCTTGGATGAACTTGTCAAGATCCTCGAGTGCAAGAAATCAAACCAACAATTGGTTCGACCAATTTTCTACAAAGTTGATCCCTCGGATATACGAAATCACAGAGGTAGCTTTGGGGAGGCACTTGCTAATCATGAATGCAAATTCAAAGATAACTTGGACAAGGTTCAGAAATGGAAGAGTGCTCTCTCAGAAGCAGCGCATTTGTCAGGGTGGCCTCTTTTCGATGAGCATTGCTCTGAACCCAGTGTTATTCATGAAATTGTTAAAGAGATTTCAGAGCAAGTTATGAACAGTACATATATGGATGTGGCCAAGTATCCAGTAGGAATAAAAACTCGGGTGCAAGAGATCCATAAGCTTTTAGGTGTTGAGGGAAGAGACGTTCATATGGTAGGGATATGGGGGGCTGGTGGAATAGGTAAGACGACAATTGCCAGAGCTGTCTATAACTCAATTGCTCATAAATTTGATGGGAGTTGTTTCTTAGAAAATGTTAGGGCAGATTCAATGGGTGCAAGAGGCTTTGTCAAACTACAGAAGACACTTCTTCGTCAGATTCTAAAGGGAAAAAAATTAAATGTGGACAATGTAGCTAGAGGAATCACTATGATCAAAGAAATGTTGCAATACAAATGTGTTCTCTTAGTGCTTGATGATGTGAATGATATTGATCAGTTAAACAACTTAGCCAGTGACTGTAGTTGGTTTGGTAAGGGTAGTAAAATCCTCATAACAACAAGGGATAGGCAATTGTTGACCAGTCATGGTGTTAATTTAATATACGAGGTCCAAAAATTTGATCCTCATGAAGCTCTCGAGCTTTTTAGTTGGAATGCCTTCAAAAAAAACGGGCCTTTGGATGGTTTTGCAGAACTTACAAAACGTGCTATACGCTATGCTCAAGGCCTTCCATTAGCTTTGACTGTTCTAGGATCTTCTCTATGTGGTGGAAATGTAGAGAAATGGCAAGCTGCATTAGATGGTTTCAAAAGCTCAAAAATTAGAGAAGTTCTCAAAATAAGTTACGATGGATTGGATGACACAGAGAAGGAAGTTTTTCTTGACATTGCATGTTTCTTCAAAGGTGAACATAGAGACCATGTGATACAAACATTAGAAGCTTGTCACTCCAAACTTGCTGAGCATTGTATCAACGTGCTCATAGACAAGGCCCTCATCAATACATCTACGGGTCGCATTTGGATGCATGACTTGCTAGAAGAACTGGGTAGATATATAGTTCACAAAGAGTCACCCAATGAACCTGGAAGGCGTAGCAGGTTGTGGTTTCATGAAGATGTTTACCGTGTTCTAACAGAGAATATTGGAACAACCAATGTTATAGGCATCAAGGTGGAGCTGCCCAAAGATTCAGATGTGATATGTTTAAGTGGGACCACTTTCTCCAACATGCAAAATCTTAGACTTTTCATACACCGTGCTGGACGCTATTCTGGCGCTGTTGATAGTCTGCCAAATAGCTTGAGGGTAGTTGATTGGCCTAAATATCCCTTACAATTTTTGCCATCCAATTTAATTCCAAGAGAACTTGCTCTAATCAATATGCCTGGCAGTCGCATCACAGCATTGGGAGATAGATACAAGCATCTGATAAATCTGACATCAATAAATCTAAGTGATTGTCAGTGCCTAAAGAAAGTGTCAGACTTATCAGGAAGCCCAAACTTACAACATTTGAATCTAGGTCTCTGTAAAAGTTTAGTTGAAGTGCATTATTCCGTTGGATTCCTCGATAAACTTGAGTATTTGAGTCTTTGGGACTGCTCAAGTCTCCAGACTTTTCCCACGGAAGTTAGTTGGAAATCCATGAGGAATCTTCAGCTTGGCTTTTGTAGAAGGCTTGAAAATTTCATAAAAATTGTGCACAACATGGAGTCCATTAAAACATTGGATCTAAGGAAAAGTGGCATAAAAGAATTGTCCTTAACGATTGAAAATCTTACTTCTTTAAGGGAATTGTACCTGTTTAGAACTCCCCTTAAGGAATTGCCTTCATCGATTGGGTATTGCACTTCCTTGAAGAAATTGAACGCGTCTGAAACTCCCATCACAGAACTGCCTTCATCAATTGGGTATCTCACGTCCTTGCAGGAATTGAATGTTTCTGAAACTTCCATCAGAGAGCTGCCTTCTTCAATTGGGAATCTCACATCCTTGGTGCAATTGAAGCTATCTAAAACTCCCATTAAAGAATTGCCCCCGTTTGGAAAACTCACGTCCTTGGTGGAATTGCACGCTTCTGAAACTCTGATTGAAGAATTGCCTCTGTCAATTGGAAATCTCAATCGCCTTCGGTCTTTAAATCTAGCATTATGTGCAAACCTTACAAATGTGCCGCACGGTGTTTACGGATGGCTGCAGCATCTACGATGGATAGACCTCTCTTGGTGTCCAAAACTGGTGACATTTCCAAGTAGGGGGAGCGCTTTGGTTTCATCAAGTGCAGAATCTCTTCCTTTGATGCTTCCATCTAATTCAAACAATGGGCATGATCATCATCCTGGTTCATTATTGTTTCCCCAGCTACAGCGTCTTGATTTTGAAGGATGCCAATTATCAGTGGTCTCTGATTTCCTAACGAATCTTGACTGTGCGTCCACATTATGGAGCCTTAATCTTTCAGGAAGCAGTTTTGATAGTCTTCCGGCATGCATCAACAAATTCAGCGAATTGAAATATCTTAATTTGGCTGGTTGCAAGAGGCTTCGAGACATTTCAGAACTTCCACCAAATATAGTAGTTATAAAGCTGGATGATTGCGTATCGTTGGAAAGATTTTCAAAATTGTCAAATATACTGGAACAGAAAGACACTCCGGGACGTGTTCGCGGGATCCACTTGTCTAATTGCAATAGACTGATGGATAGTCTTGGCATGGACGTGGTGTCGAAGATGGCAAAAGCATTGTTTAATCAGCTGGTGCATGCCGGCTTAGATCAGCATGTTCAGTGGAATCTTATTCTTCCAAGCCTCCAGGAAATTGAAGTTCCAAATTGGTTTGATGGGGGTGAGGTGGACACAAGTCTGCTTCCTGGTCAGGACCCTGAGTATGATATATGTGAAATTTTGATAAAAATCCCCAGGAATCTGAAGGCTGAGAGAATAGGATTGGTTGTCTGTGTTGTTTTTGAACTTACCCAAGATTTTTTATATGATCCTGATTGTTGTTTTGTGGAGGTTGTGATTGATAAAAAAGTGTGTCCTGACTCTGGTTGCCACTTTTTTAAATCAAAAGCGACATGTATTGCATCAGCTCAAGATCATGTATTAGTGCATCTGACATTTATTGCTTTCAAGGAGCTAGAGGTGGTGGATCCTGTGGTTCGAGTGATTTTTCATGACTATGCCTCAGGTGGCAAAGGGTTGCTCCTAAAGAGTTTTGGGGTCCACCTGGCTAATATGAAAGACCTGGCACGAGAAAGATATAGGCAGAGCAATGCTAGCATTTCTGATCAGGTGGCTTTGCACGCAGAAGGAGCAGCTGTAGCATCATTAGTATCAGATGACAGCAATTACGGGGAAGTACACGATGATCATCACAACGGGGAACAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0006073 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009311 GO:0009505 GO:0009628 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016020 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0035251 GO:0036293 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070482 GO:0071695 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

1285

Amino Acids

144.07

Weight (kDa)

6.39

Isoelectric Point (pI)

37.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	22 - 192	2.5e-54	TIR domain
TIR_2	PF13676	25 - 120	2.7e-16	TIR domain
NB-ARC	PF00931	205 - 376	5.9e-21	NB-ARC domain
WHD_ROQ1	PF23282	441 - 511	2.7e-14	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	694 - 801	1.2e-12	Leucine-rich repeat region
LRR_14	PF23598	799 - 896	1.7e-11	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49190 AT5G49190 AT5G49190
fragaria_vesca	FvH4_4g18710
malus_domestica	MD13G1164000.v1.1 MD13G1164100.v1.1 MD13G1164200.v1.1 MD16G1164000.v1.1
prunus_persica	Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1
pyrus_communis	pycom13g14010 pycom16g13860 pycom16g13870
rosa_chinensis	RchiOBHm_Chr4g0423411
rosa_laevigata	RLG00000007559
rosa_multiflora	Rmu_sc0000893.1_g000010 Rmu_ssc0000174.1_g000013
rosa_roxburghii	Rroxscaffold_4G00291020 Rroxscaffold_5G00365630
rosa_rugosa	Rorug04G0187400 Rorug04G0187500 Rorug04G0187600 Rorug04G0187700 Rorug07G0218200
rosa_samantha	Rh3BG353800 Rh4AG246400 Rh4BG251800 Rh4CG260300 Rh4DG247200 Rh6BG052100
rosa_wichuraiana	Rw1G026460 Rw4G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3044
Acc36I	ACCTGC	1 cut(s)	1548
AccB7I	CCANNNNNTGG	2 cut(s)	328, 2904
AccII	CGCG	2 cut(s)	2333, 3129
AciI	CCGC	2 cut(s)	2636, 3129
AcoI	YGGCCR	1 cut(s)	588
AcsI	RAATTY	6 cut(s)	419, 743, 1073, 2146, 2960, 3363
AcuI	CTGAAG	2 cut(s)	2102, 3410
AfaI	GTAC	3 cut(s)	574, 2261, 3825
AfeI	AGCGCT	1 cut(s)	2725
AfiI	CCNNNNNNNGG	8 cut(s)	60, 273, 328, 1139, 2504, 2717, 2904, 3509
AflII	CTTAAG	1 cut(s)	2279
AflIII	ACRYGT	3 cut(s)	2331, 3121, 3539
AhdI	GACNNNNNGTC	2 cut(s)	2893, 3184
AjiI	CACGTC	4 cut(s)	2382, 2517, 3122, 3181
AjnI	CCWGG	8 cut(s)	1543, 1846, 2800, 3273, 3331, 3380, 3690, 3708
AjuI	GAANNNNNNNTTGG	2 cut(s)	315, 347
AloI	GAACNNNNNNTCC	2 cut(s)	645, 677
Alw21I	GWGCWC	3 cut(s)	463, 1440, 2166
Alw26I	GTCTC	6 cut(s)	651, 1368, 1991, 2075, 2869, 3003
Alw44I	GTGCAC	1 cut(s)	2162
AlwNI	CAGNNNCTG	1 cut(s)	975
Ama87I	CYCGRG	3 cut(s)	302, 615, 1094
Aor51HI	AGCGCT	1 cut(s)	2725
AoxI	GGCC	7 cut(s)	118, 491, 588, 1130, 1183, 1449, 1778
ApaLI	GTGCAC	1 cut(s)	2162
ApeKI	GCWGC	7 cut(s)	473, 1250, 1639, 2425, 2656, 2659, 3779
ApoI	RAATTY	6 cut(s)	419, 743, 1073, 2146, 2960, 3363
Asp700I	GAANNNNTTC	3 cut(s)	1115, 1335, 2751
AspLEI	GCGC	3 cut(s)	478, 1739, 2726
AspS9I	GGNCC	6 cut(s)	1066, 1130, 1450, 1674, 3340, 3685
AsuC2I	CCSGG	1 cut(s)	3117
AsuHPI	GGTGA	6 cut(s)	16, 104, 1376, 1524, 2713, 3320
AsuNHI	GCTAGC	1 cut(s)	3740
AvaI	CYCGRG	3 cut(s)	302, 615, 1094
AvaII	GGWCC	4 cut(s)	1066, 1674, 3340, 3685
AxyI	CCTNAGG	1 cut(s)	3649
BaeGI	GKGCMC	1 cut(s)	2166
BalI	TGGCCA	1 cut(s)	590
BamHI	GGATCC	2 cut(s)	3132, 3613
BauI	CACGAG	1 cut(s)	3714
BbsI	GAAGAC	2 cut(s)	824, 2933
Bbv12I	GWGCWC	3 cut(s)	463, 1440, 2166
BbvI	GCAGC	7 cut(s)	485, 1237, 1626, 2412, 2643, 2671, 3791
BciT130I	CCWGG	8 cut(s)	1545, 1848, 2802, 3275, 3333, 3382, 3692, 3710
BciVI	GTATCC	2 cut(s)	91, 606
BclI	TGATCA	7 cut(s)	6, 169, 888, 949, 2791, 3751, 3832
BcnI	CCSGG	1 cut(s)	3117
BcoDI	GTCTC	6 cut(s)	651, 1368, 1991, 2075, 2869, 3003
BfaI	CTAG	7 cut(s)	873, 1208, 1496, 1982, 2606, 3602, 3741
BfmI	CTRYAG	5 cut(s)	812, 976, 2657, 2825, 3783
BfoI	RGCGCY	2 cut(s)	1740, 2727
BfrI	CTTAAG	1 cut(s)	2279
BfuAI	ACCTGC	1 cut(s)	1548
BfuI	GTATCC	2 cut(s)	91, 606
BisI	GCNGC	8 cut(s)	474, 1251, 1640, 2426, 2636, 2657, 2660, 3780
BlpI	GCTNAGC	1 cut(s)	1419
BlsI	GCNGC	8 cut(s)	475, 1252, 1641, 2427, 2637, 2658, 2661, 3781
Bme1390I	CCNGG	9 cut(s)	1545, 1848, 2802, 3117, 3275, 3333, 3382, 3692, 3710
Bme18I	GGWCC	4 cut(s)	1066, 1674, 3340, 3685
BmeRI	GACNNNNNGTC	2 cut(s)	2893, 3184
BmeT110I	CYCGRG	3 cut(s)	302, 615, 1094
BmgBI	CACGTC	4 cut(s)	2382, 2517, 3122, 3181
BmgT120I	GGNCC	6 cut(s)	1066, 1130, 1450, 1674, 3340, 3685
BmiI	GGNNCC	6 cut(s)	1675, 2909, 3134, 3342, 3615, 3686
BmrFI	CCNGG	9 cut(s)	1545, 1848, 2802, 3117, 3275, 3333, 3382, 3692, 3710
BmrI	ACTGGG	2 cut(s)	516, 1514
BmtI	GCTAGC	1 cut(s)	3744
BmuI	ACTGGG	2 cut(s)	516, 1514
BoxI	GACNNNNGTC	1 cut(s)	3321
BpiI	GAAGAC	2 cut(s)	824, 2933
BplI	GAGNNNNNCTC	4 cut(s)	497, 529, 1275, 1307
BpmI	CTGGAG	2 cut(s)	2057, 3257
Bpu1102I	GCTNAGC	1 cut(s)	1419
BpuEI	CTTGAG	5 cut(s)	1164, 1783, 2050, 2060, 3540
BpuMI	CCSGG	1 cut(s)	3117
Bsa29I	ATCGAT	1 cut(s)	2297
BsaBI	GATNNNNATC	5 cut(s)	236, 948, 2796, 3374, 3834
BsaI	GGTCTC	3 cut(s)	1368, 1991, 2869
BsaJI	CCNNGG	6 cut(s)	59, 360, 2085, 2454, 2520, 3380
BsaXI	ACNNNNNCTCC	2 cut(s)	3768, 3798
Bsc4I	CCNNNNNNNGG	8 cut(s)	60, 273, 328, 1139, 2504, 2717, 2904, 3509
Bse118I	RCCGGY	1 cut(s)	3227
Bse1I	ACTGG	7 cut(s)	522, 599, 969, 1038, 1509, 2703, 3102
Bse21I	CCTNAGG	1 cut(s)	3649
Bse3DI	GCAATG	3 cut(s)	508, 1344, 3742
Bse8I	GATNNNNATC	5 cut(s)	236, 948, 2796, 3374, 3834
BseBI	CCWGG	8 cut(s)	1545, 1848, 2802, 3275, 3333, 3382, 3692, 3710
BseCI	ATCGAT	1 cut(s)	2297
BseDI	CCNNGG	6 cut(s)	59, 360, 2085, 2454, 2520, 3380
BseJI	GATNNNNATC	5 cut(s)	236, 948, 2796, 3374, 3834
BseLI	CCNNNNNNNGG	8 cut(s)	60, 273, 328, 1139, 2504, 2717, 2904, 3509
BseMI	GCAATG	3 cut(s)	508, 1344, 3742
BseMII	CTCAG	6 cut(s)	190, 480, 1410, 3336, 3387, 3663
BseNI	ACTGG	7 cut(s)	522, 599, 969, 1038, 1509, 2703, 3102
BseRI	GAGGAG	1 cut(s)	198
BseSI	GKGCMC	1 cut(s)	2166
BseXI	GCAGC	7 cut(s)	485, 1237, 1626, 2412, 2643, 2671, 3791
BseYI	CCCAGC	1 cut(s)	2820
BsgI	GTGCAG	1 cut(s)	2763
Bsh1236I	CGCG	2 cut(s)	2333, 3129
BshFI	GGCC	7 cut(s)	120, 493, 590, 1132, 1185, 1451, 1780
BshVI	ATCGAT	1 cut(s)	2297
BsiHKAI	GWGCWC	3 cut(s)	463, 1440, 2166
BsiHKCI	CYCGRG	3 cut(s)	302, 615, 1094
BsiSI	CCGG	3 cut(s)	2945, 3116, 3228
BslFI	GGGAC	3 cut(s)	1687, 2072, 3132
BslI	CCNNNNNNNGG	8 cut(s)	60, 273, 328, 1139, 2504, 2717, 2904, 3509
BsmAI	GTCTC	6 cut(s)	651, 1368, 1991, 2075, 2869, 3003
BsmBI	CGTCTC	1 cut(s)	651
BsmFI	GGGAC	3 cut(s)	1687, 2072, 3132
BsmI	GAATGC	2 cut(s)	419, 1117
BsnI	GGCC	7 cut(s)	120, 493, 590, 1132, 1185, 1451, 1780
Bso31I	GGTCTC	3 cut(s)	1368, 1991, 2869
BsoBI	CYCGRG	3 cut(s)	302, 615, 1094
Bsp1286I	GDGCHC	3 cut(s)	463, 1440, 2166
Bsp1720I	GCTNAGC	1 cut(s)	1419
Bsp19I	CCATGG	1 cut(s)	59
BspACI	CCGC	2 cut(s)	2636, 3129
BspANI	GGCC	7 cut(s)	120, 493, 590, 1132, 1185, 1451, 1780
BspCNI	CTCAG	6 cut(s)	189, 479, 1411, 3337, 3388, 3662
BspDI	ATCGAT	1 cut(s)	2297
BspFNI	CGCG	2 cut(s)	2333, 3129
BspHI	TCATGA	5 cut(s)	409, 532, 1084, 1570, 3637
BspLI	GGNNCC	6 cut(s)	1675, 2909, 3134, 3342, 3615, 3686
BspMAI	CTGCAG	1 cut(s)	2661
BspMI	ACCTGC	1 cut(s)	1548
BspOI	GCTAGC	1 cut(s)	3744
BspTI	CTTAAG	1 cut(s)	2279
BspTNI	GGTCTC	3 cut(s)	1368, 1991, 2869
BsrDI	GCAATG	3 cut(s)	508, 1344, 3742
BsrFI	RCCGGY	1 cut(s)	3227
BsrI	ACTGG	7 cut(s)	522, 599, 969, 1038, 1509, 2703, 3102
BssAI	RCCGGY	1 cut(s)	3227
BssECI	CCNNGG	6 cut(s)	59, 360, 2085, 2454, 2520, 3380
BssSI	CACGAG	1 cut(s)	3714
BssT1I	CCWWGG	3 cut(s)	59, 2454, 2520
Bst2BI	CACGAG	1 cut(s)	3714
Bst2UI	CCWGG	8 cut(s)	1545, 1848, 2802, 3275, 3333, 3382, 3692, 3710
Bst4CI	ACNGT	8 cut(s)	225, 572, 977, 1204, 1586, 1718, 2642, 2891
Bst6I	CTCTTC	4 cut(s)	28, 449, 649, 2757
BstAFI	CTTAAG	1 cut(s)	2279
BstAPI	GCANNNNNTGC	2 cut(s)	1492, 2615
BstC8I	GCNNGC	7 cut(s)	1248, 2535, 2950, 3225, 3229, 3742, 3768
BstDSI	CCRYGG	2 cut(s)	59, 2085
BstFNI	CGCG	2 cut(s)	2333, 3129
BstH2I	RGCGCY	2 cut(s)	1740, 2727
BstHHI	GCGC	3 cut(s)	478, 1739, 2726
BstMAI	GTCTC	6 cut(s)	651, 1368, 1991, 2075, 2869, 3003
BstMWI	GCNNNNNNNGC	9 cut(s)	126, 1492, 1734, 2467, 2615, 3741, 3767, 3776, 3785
BstNI	CCWGG	8 cut(s)	1545, 1848, 2802, 3275, 3333, 3382, 3692, 3710
BstNSI	RCATGY	6 cut(s)	1353, 1693, 2952, 3227, 3245, 3543
BstPAI	GACNNNNGTC	1 cut(s)	3321
BstSCI	CCNGG	9 cut(s)	1543, 1846, 2800, 3115, 3273, 3331, 3380, 3690, 3708
BstSFI	CTRYAG	5 cut(s)	812, 976, 2657, 2825, 3783
BstSLI	GKGCMC	1 cut(s)	2166
BstUI	CGCG	2 cut(s)	2333, 3129
BstV1I	GCAGC	7 cut(s)	485, 1237, 1626, 2412, 2643, 2671, 3791
BstV2I	GAAGAC	2 cut(s)	824, 2933
BstX2I	RGATCY	6 cut(s)	297, 627, 1211, 2191, 3132, 3613
BstYI	RGATCY	6 cut(s)	297, 627, 1211, 2191, 3132, 3613
Bsu15I	ATCGAT	1 cut(s)	2297
Bsu36I	CCTNAGG	1 cut(s)	3649
BsuI	GTATCC	2 cut(s)	91, 606
BsuRI	GGCC	7 cut(s)	120, 493, 590, 1132, 1185, 1451, 1780
BsuTUI	ATCGAT	1 cut(s)	2297
BtgI	CCRYGG	2 cut(s)	59, 2085
BtrI	CACGTC	4 cut(s)	2382, 2517, 3122, 3181
BtsIMutI	CAGTG	5 cut(s)	529, 976, 1931, 2865, 3254
BveI	ACCTGC	1 cut(s)	1548
Cac8I	GCNNGC	7 cut(s)	1248, 2535, 2950, 3225, 3229, 3742, 3768
CaiI	CAGNNNCTG	1 cut(s)	975
CciI	TCATGA	5 cut(s)	409, 532, 1084, 1570, 3637
CfoI	GCGC	3 cut(s)	478, 1739, 2726
Cfr10I	RCCGGY	1 cut(s)	3227
Cfr13I	GGNCC	6 cut(s)	1066, 1130, 1450, 1674, 3340, 3685
ClaI	ATCGAT	1 cut(s)	2297
CseI	GACGC	6 cut(s)	128, 140, 1734, 2322, 2819, 2883
Csp6I	GTAC	3 cut(s)	573, 2260, 3824
CviQI	GTAC	3 cut(s)	573, 2260, 3824
DraI	TTTAAA	2 cut(s)	2601, 3526
DriI	GACNNNNNGTC	2 cut(s)	2893, 3184
EaeI	YGGCCR	1 cut(s)	588
Eam1104I	CTCTTC	4 cut(s)	28, 449, 649, 2757
Eam1105I	GACNNNNNGTC	2 cut(s)	2893, 3184
EarI	CTCTTC	4 cut(s)	28, 449, 649, 2757
Eco130I	CCWWGG	3 cut(s)	59, 2454, 2520
Eco147I	AGGCCT	1 cut(s)	1185
Eco31I	GGTCTC	3 cut(s)	1368, 1991, 2869
Eco47I	GGWCC	4 cut(s)	1066, 1674, 3340, 3685
Eco47III	AGCGCT	1 cut(s)	2725
Eco57I	CTGAAG	2 cut(s)	2102, 3410
Eco81I	CCTNAGG	1 cut(s)	3649
Eco88I	CYCGRG	3 cut(s)	302, 615, 1094
EcoO109I	RGGNCCY	2 cut(s)	1450, 3340
EcoRII	CCWGG	8 cut(s)	1543, 1846, 2800, 3273, 3331, 3380, 3690, 3708
EcoT14I	CCWWGG	3 cut(s)	59, 2454, 2520
EcoT22I	ATGCAT	3 cut(s)	265, 1488, 2954
ErhI	CCWWGG	3 cut(s)	59, 2454, 2520
Esp3I	CGTCTC	1 cut(s)	651
FalI	AAGNNNNNCTT	4 cut(s)	263, 295, 1323, 1355
FaqI	GGGAC	3 cut(s)	1687, 2072, 3132
FauI	CCCGC	1 cut(s)	3122
FauNDI	CATATG	1 cut(s)	666
FbaI	TGATCA	7 cut(s)	6, 169, 888, 949, 2791, 3751, 3832
Fnu4HI	GCNGC	8 cut(s)	474, 1251, 1640, 2426, 2636, 2657, 2660, 3780
Fsp4HI	GCNGC	8 cut(s)	474, 1251, 1640, 2426, 2636, 2657, 2660, 3780
FspBI	CTAG	7 cut(s)	873, 1208, 1496, 1982, 2606, 3602, 3741
GlaI	GCGC	3 cut(s)	477, 1738, 2725
GluI	GCNGC	8 cut(s)	474, 1251, 1640, 2426, 2636, 2657, 2660, 3780
GsaI	CCCAGC	1 cut(s)	2824
GsuI	CTGGAG	2 cut(s)	2057, 3257
HaeII	RGCGCY	2 cut(s)	1740, 2727
HaeIII	GGCC	7 cut(s)	120, 493, 590, 1132, 1185, 1451, 1780
HapII	CCGG	3 cut(s)	2945, 3116, 3228
HgaI	GACGC	6 cut(s)	128, 140, 1734, 2322, 2819, 2883
HhaI	GCGC	3 cut(s)	478, 1739, 2726
Hin6I	GCGC	3 cut(s)	476, 1737, 2724
HinP1I	GCGC	3 cut(s)	476, 1737, 2724
HincII	GTYRAC	1 cut(s)	1035
HindII	GTYRAC	1 cut(s)	1035
HindIII	AAGCTT	2 cut(s)	635, 1398
HpaII	CCGG	3 cut(s)	2945, 3116, 3228
HphI	GGTGA	6 cut(s)	16, 104, 1376, 1524, 2713, 3320
Hpy99I	CGWCG	1 cut(s)	134
HpyCH4III	ACNGT	8 cut(s)	225, 572, 977, 1204, 1586, 1718, 2642, 2891
HpyCH4IV	ACGT	7 cut(s)	660, 1162, 1434, 2381, 2516, 3121, 3180
HpyF10VI	GCNNNNNNNGC	9 cut(s)	126, 1492, 1734, 2467, 2615, 3741, 3767, 3776, 3785
HpySE526I	ACGT	7 cut(s)	660, 1162, 1434, 2381, 2516, 3121, 3180
HspAI	GCGC	3 cut(s)	476, 1737, 2724
KroI	GCCGGC	1 cut(s)	3227
KroNI	GCCGGC	1 cut(s)	3229
Ksp22I	TGATCA	7 cut(s)	6, 169, 888, 949, 2791, 3751, 3832
LmnI	GCTCC	6 cut(s)	1636, 2721, 2907, 3598, 3672, 3776
Lsp1109I	GCAGC	7 cut(s)	485, 1237, 1626, 2412, 2643, 2671, 3791
MaeI	CTAG	7 cut(s)	873, 1208, 1496, 1982, 2606, 3602, 3741
MaeII	ACGT	7 cut(s)	660, 1162, 1434, 2381, 2516, 3121, 3180
MaeIII	GTNAC	5 cut(s)	971, 1301, 1403, 1530, 2701
MfeI	CAATTG	9 cut(s)	216, 326, 708, 732, 1028, 2367, 2436, 2462, 2571
MflI	RGATCY	6 cut(s)	297, 627, 1211, 2191, 3132, 3613
MhlI	GDGCHC	3 cut(s)	463, 1440, 2166
MlsI	TGGCCA	1 cut(s)	590
MluI	ACGCGT	1 cut(s)	2331
MluNI	TGGCCA	1 cut(s)	590
MlyI	GAGTC	4 cut(s)	1538, 2059, 2183, 3500
MmeI	TCCRAC	5 cut(s)	1088, 1710, 2002, 2078, 3048
Mox20I	TGGCCA	1 cut(s)	590
Mph1103I	ATGCAT	3 cut(s)	265, 1488, 2954
MroNI	GCCGGC	1 cut(s)	3227
MroXI	GAANNNNTTC	3 cut(s)	1115, 1335, 2751
MscI	TGGCCA	1 cut(s)	590
MslI	CAYNNNNRTG	6 cut(s)	161, 1481, 1575, 1631, 3179, 3642
Msp20I	TGGCCA	1 cut(s)	590
MspA1I	CMGCKG	2 cut(s)	3217, 3782
MspCI	CTTAAG	1 cut(s)	2279
MspI	CCGG	3 cut(s)	2945, 3116, 3228
MspR9I	CCNGG	9 cut(s)	1545, 1848, 2802, 3117, 3275, 3333, 3382, 3692, 3710
MunI	CAATTG	9 cut(s)	216, 326, 708, 732, 1028, 2367, 2436, 2462, 2571
Mva1269I	GAATGC	2 cut(s)	419, 1117
MvaI	CCWGG	8 cut(s)	1545, 1848, 2802, 3275, 3333, 3382, 3692, 3710
MvnI	CGCG	2 cut(s)	2333, 3129
MwoI	GCNNNNNNNGC	9 cut(s)	126, 1492, 1734, 2467, 2615, 3741, 3767, 3776, 3785
NaeI	GCCGGC	1 cut(s)	3229
NciI	CCSGG	1 cut(s)	3117
NcoI	CCATGG	1 cut(s)	59
NdeI	CATATG	1 cut(s)	666
NgoMIV	GCCGGC	1 cut(s)	3227
NheI	GCTAGC	1 cut(s)	3740
NlaIV	GGNNCC	6 cut(s)	1675, 2909, 3134, 3342, 3615, 3686
NmuCI	GTSAC	4 cut(s)	971, 1403, 1530, 2701
NsiI	ATGCAT	3 cut(s)	265, 1488, 2954
NspI	RCATGY	6 cut(s)	1353, 1693, 2952, 3227, 3245, 3543
PaeI	GCATGC	2 cut(s)	2952, 3227
PaeR7I	CTCGAG	2 cut(s)	302, 1094
PagI	TCATGA	5 cut(s)	409, 532, 1084, 1570, 3637
PceI	AGGCCT	1 cut(s)	1185
PciI	ACATGT	1 cut(s)	3539
PctI	GAATGC	2 cut(s)	419, 1117
PdiI	GCCGGC	1 cut(s)	3229
PdmI	GAANNNNTTC	3 cut(s)	1115, 1335, 2751
PflMI	CCANNNNNTGG	2 cut(s)	328, 2904
PfoI	TCCNGGA	2 cut(s)	3115, 3273
PkrI	GCNGC	8 cut(s)	475, 1252, 1641, 2427, 2637, 2658, 2661, 3781
PleI	GAGTC	4 cut(s)	1537, 2058, 2182, 3500
PpsI	GAGTC	4 cut(s)	1537, 2058, 2182, 3500
PpuMI	RGGWCCY	1 cut(s)	3340
PscI	ACATGT	1 cut(s)	3539
PshAI	GACNNNNGTC	1 cut(s)	3321
PsiI	TTATAA	1 cut(s)	3044
Psp5II	RGGWCCY	1 cut(s)	3340
Psp6I	CCWGG	8 cut(s)	1543, 1846, 2800, 3273, 3331, 3380, 3690, 3708
PspFI	CCCAGC	1 cut(s)	2820
PspGI	CCWGG	8 cut(s)	1543, 1846, 2800, 3273, 3331, 3380, 3690, 3708
PspN4I	GGNNCC	6 cut(s)	1675, 2909, 3134, 3342, 3615, 3686
PspPI	GGNCC	6 cut(s)	1066, 1130, 1450, 1674, 3340, 3685
PspPPI	RGGWCCY	1 cut(s)	3340
PspXI	VCTCGAGB	1 cut(s)	302
PstI	CTGCAG	1 cut(s)	2661
PstNI	CAGNNNCTG	1 cut(s)	975
PsuI	RGATCY	6 cut(s)	297, 627, 1211, 2191, 3132, 3613
PvuII	CAGCTG	2 cut(s)	3217, 3782
RsaI	GTAC	3 cut(s)	574, 2261, 3825
RsaNI	GTAC	3 cut(s)	573, 2260, 3824
RseI	CAYNNNNRTG	6 cut(s)	161, 1481, 1575, 1631, 3179, 3642
SatI	GCNGC	8 cut(s)	474, 1251, 1640, 2426, 2636, 2657, 2660, 3780
Sau96I	GGNCC	6 cut(s)	1066, 1130, 1450, 1674, 3340, 3685
SchI	GAGTC	4 cut(s)	1538, 2059, 2183, 3500
ScrFI	CCNGG	9 cut(s)	1545, 1848, 2802, 3117, 3275, 3333, 3382, 3692, 3710
SduI	GDGCHC	3 cut(s)	463, 1440, 2166
SfcI	CTRYAG	5 cut(s)	812, 976, 2657, 2825, 3783
Sfr274I	CTCGAG	2 cut(s)	302, 1094
SinI	GGWCC	4 cut(s)	1066, 1674, 3340, 3685
SlaI	CTCGAG	2 cut(s)	302, 1094
SmiMI	CAYNNNNRTG	6 cut(s)	161, 1481, 1575, 1631, 3179, 3642
SmlI	CTYRAG	8 cut(s)	302, 1094, 1179, 1762, 2039, 2065, 2279, 3555
SmoI	CTYRAG	8 cut(s)	302, 1094, 1179, 1762, 2039, 2065, 2279, 3555
SphI	GCATGC	2 cut(s)	2952, 3227
SseBI	AGGCCT	1 cut(s)	1185
SsiI	CCGC	2 cut(s)	2636, 3129
SspI	AATATT	1 cut(s)	1603
SspMI	CTAG	7 cut(s)	873, 1208, 1496, 1982, 2606, 3602, 3741
StuI	AGGCCT	1 cut(s)	1185
StyD4I	CCNGG	9 cut(s)	1543, 1846, 2800, 3115, 3273, 3331, 3380, 3690, 3708
StyI	CCWWGG	3 cut(s)	59, 2454, 2520
TaaI	ACNGT	8 cut(s)	225, 572, 977, 1204, 1586, 1718, 2642, 2891
TaiI	ACGT	7 cut(s)	663, 1165, 1437, 2384, 2519, 3124, 3183
TaqII	GACCGA	1 cut(s)	2583
TatI	WGTACW	2 cut(s)	572, 3823
TauI	GCSGC	1 cut(s)	2638
TscAI	CASTG	5 cut(s)	529, 976, 1931, 2865, 3254
TseFI	GTSAC	4 cut(s)	971, 1403, 1530, 2701
TseI	GCWGC	7 cut(s)	473, 1250, 1639, 2425, 2656, 2659, 3779
Tsp45I	GTSAC	4 cut(s)	971, 1403, 1530, 2701
TspGWI	ACGGA	3 cut(s)	2008, 2102, 2664
TspRI	CASTG	5 cut(s)	529, 976, 1931, 2865, 3254
Van91I	CCANNNNNTGG	2 cut(s)	328, 2904
Vha464I	CTTAAG	1 cut(s)	2279
VneI	GTGCAC	1 cut(s)	2162
VpaK11BI	GGWCC	4 cut(s)	1066, 1674, 3340, 3685
XapI	RAATTY	6 cut(s)	419, 743, 1073, 2146, 2960, 3363
XceI	RCATGY	6 cut(s)	1353, 1693, 2952, 3227, 3245, 3543
XcmI	CCANNNNNNNNNTGG	2 cut(s)	277, 2185
XhoI	CTCGAG	2 cut(s)	302, 1094
XmnI	GAANNNNTTC	3 cut(s)	1115, 1335, 2751
XspI	CTAG	7 cut(s)	873, 1208, 1496, 1982, 2606, 3602, 3741
Zsp2I	ATGCAT	3 cut(s)	265, 1488, 2954

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.