Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0003721

Physical Location & Seq

Genomic Coordinates Reverse (-)

42175615 .. 42181180

Gene Structure 5566 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG251800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGCGGGAGAGGATTCAGGACACGCTCGCCGCACACCGGAACGAACTTGTTTCTCTTCTCTCCAGGTATGTTGCTCGAGGAAATGGGATACTACAGCCTCATCAGATAATCAACGAGCTCGAAAAGGTGATCGAAGAGGATGAGAAGATGAAGAAGCTAAAGGATAGCCCCTTCAGCAAAGTTCTACAGTCTGCACAGGAAGCAATTGTTCTGCCTCCTTTTGTGGCTTTTGCTCTTCGTCCAAGACCTGGTGTATGGGAATATGTCCGTGTCAATGTATATGAACTCAGTGTGGATCATCTGAGTGTTGCAGAGTATCTCCTGTTCAAAGAAGAACTTCTGGATGGAGAGTGCAATGACAATTATGTGCTCGAACTTGATTTGGAGCCATTTAATGCAACATTCCCTCGGCCAACCCGTTCATCATCCATTGGGAATGGGGTTCAATTCCTTAACCGTCACTTATCTTCAATTATGTTCCGTAACAAAGAAAGTTTGGAGCCTCTTCTTGATTTCCTTCGTACGCATAAACATGATGGCCATGCAATGATGCTGAACGATCGAATTCAGAGCATACCCAAGCTTCAGTCTGCTTTAGCCAAGGCAGAGGAATATCTTTCTAAGTTCCCCCCAATGACACCATATTCTGAGTTTGAGTTTGAGTTACAAGGAATGGGATTTGAGAGAGGGTGGGGTGACACTGTAGGACGGATGTCAGAGATGGTGCATCTACTCTTGGAAATTCTTCAGGCTCCAGATCCTTCTACACTCGAAACATTTCTTGGCAGGATTCCTATGGTGTTCAATGTTGTTATTGTGTCTCCACACGGCTACTTTGGCCAAGCAAATGTATTGGGTTTGCCGGACACAGGTGGGCAGGTTGTTTATATACTGGATCAAGTGCGGGCCCTGGAGAATGAGATGCTTCTTAGAATACAGAAGCAAGGACTGGATGTTATTCCAAAGATTCTGATTGTTACTCGACTGCTACCTGATGCAAAAGGAACAACATGCAACCAGAGATTGGAAAGAGTTAGTGGTACAGAAAACACATATATCTTGCGGGTACCCTTCAGGACTAAGAATGGAATTCTCCGGAAATGGATTTCAAGGTTTGATGTCTGGCCTTACTTGGAGACCTTTGCAGAGGATGCATCAAACGAAATTGCCGCTGAGCTACAGGGTGTTCCAGATCTGATCATTGGAAACTACAGTGATGGAAATCTTGTTTCAACTTTGTTATCTTATAAACTAGGAATCACACAGTGCAACATTGCTCATGCATTGGAGAAGACAAAGTACCCAGATTCTGATATATTTTGGAAAAGTTACGAGGACAAGTACCATTTCTCAAGTCAATTCACTGCCGATCTCATTGCAATGAACAATGCAGATTTCATAGTCACCAGTACATACCAAGAGATTGCGGGAAGCCAGAACAATGTTGGACAGTATGAGAGCCACACAGCTTTCACTCTTCCTGGGCTGTATCGAGTTGTTCACGGGGTTGATGTTTTTGATCCTAAGTTTAATATTGTGTCTCCAGGAGCAGATATGTGCATATATTTTTCATATTCTGACAGGGAAAAAAGACTTACAGCACTGCATGGCACAATTGAAGAACTTATATATGGGGCTGAGCAGAATGATGAGCATATTGGGTTCTTGGCTGATCGATCAAAGCCTACTGTGTTTTCCATGGCAAGACTTGATAGAGTGAAAAATTTAACTGGACTCGTTGAGTGCTATGGTAAGAGCACCAAACTAAGAGAAACGGTAAATCTTGTTGTGGTTGGTGGTTACATGGACTCCAAGAACTCTAGGGATAGAGAAGAAATGGAAGAGATTGAAAAGATGCATGACCTCATTAAGAAGTATGACTTGAATGGCCAGTTTCGATGGATAGCGGCCCAAATGAACCGGGCCCGTAATGGTGAGCTGTACCGCTACATTGCAGACACAAGAGGCGTCTTTGTGCAGCCTGCTTTCTACGAAGCTTTTGGCCTCACTGTTGTGGAAGCCATGACTTGTGGCCTTCCTACTTTTGCCACTAGTCATGGTGGCCCTGCTGAGATCATTGAGCATGGTGTCTCAGGGTTCCATATTGATCCCTATCACCCTGATCAAGTGGCTGAAACTCTGATCGACTTCTTTGGTCAGTGCCAAAAGGATCCTAGCCTCTGGGAAAAGATATCTGGAGCCGGGCTTAAACGAATTTATGAAAGGTATCATATTCTTGGCCGACTTTCAGATAGACCATCTTGGAGTCTTGGACAATGTTTGTCAACCTCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0006073 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009311 GO:0009505 GO:0009628 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016020 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0035251 GO:0036293 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070482 GO:0071695 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

765

Amino Acids

86.84

Weight (kDa)

5.5

Isoelectric Point (pI)

38.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	1 - 111	1e-47	Sucrose synthase N-terminal
SUS_EPBD	PF24862	146 - 233	2.5e-35	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	256 - 544	4.9e-165	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	557 - 717	4e-30	Glycosyl transferases group 1
GT4-conflict	PF20706	558 - 703	1.4e-06	Family 4 Glycosyltransferase in conflict systems
Glyco_trans_1_4	PF13692	562 - 716	4.6e-16	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49190 AT5G49190 AT5G49190
fragaria_vesca	FvH4_4g18710
malus_domestica	MD13G1164000.v1.1 MD13G1164100.v1.1 MD13G1164200.v1.1 MD16G1164000.v1.1
prunus_persica	Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1
pyrus_communis	pycom13g14010 pycom16g13860 pycom16g13870
rosa_chinensis	RchiOBHm_Chr4g0423411
rosa_laevigata	RLG00000007559
rosa_multiflora	Rmu_sc0000893.1_g000010 Rmu_ssc0000174.1_g000013
rosa_roxburghii	Rroxscaffold_4G00291020 Rroxscaffold_5G00365630
rosa_rugosa	Rorug04G0187400 Rorug04G0187500 Rorug04G0187600 Rorug04G0187700 Rorug07G0218200
rosa_samantha	Rh3BG353800 Rh4AG246400 Rh4BG251800 Rh4CG260300 Rh4DG247200 Rh6BG052100
rosa_wichuraiana	Rw1G026460 Rw4G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1248
Acc36I	ACCTGC	1 cut(s)	868
Acc65I	GGTACC	1 cut(s)	1066
AccB1I	GGYRCC	1 cut(s)	1066
AccB7I	CCANNNNNTGG	2 cut(s)	248, 1024
AccIII	TCCGGA	1 cut(s)	1095
AciI	CCGC	8 cut(s)	4, 30, 904, 1063, 1170, 1427, 1907, 1945
AclWI	GGATC	7 cut(s)	303, 752, 903, 1514, 2102, 2165, 2178
AcoI	YGGCCR	5 cut(s)	410, 538, 838, 1888, 2239
AcsI	RAATTY	5 cut(s)	564, 741, 1089, 1723, 2214
AcuI	CTGAAG	4 cut(s)	157, 569, 731, 1057
AcyI	GRCGYC	1 cut(s)	1968
AdeI	CACNNNGTG	1 cut(s)	1266
AfaI	GTAC	7 cut(s)	523, 1042, 1068, 1301, 1343, 1411, 1943
AfiI	CCNNNNNNNGG	4 cut(s)	36, 248, 869, 1024
AgsI	TTSAA	9 cut(s)	328, 446, 471, 805, 1110, 1233, 1619, 1850, 1885
AhlI	ACTAGT	1 cut(s)	2051
AjnI	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
AjuI	GAANNNNNNNTTGG	2 cut(s)	765, 797
AleI	CACNNNNGTG	1 cut(s)	2012
AluBI	AGCT	7 cut(s)	118, 157, 583, 1177, 1469, 1939, 1997
AluI	AGCT	7 cut(s)	118, 157, 583, 1177, 1469, 1939, 1997
Alw21I	GWGCWC	3 cut(s)	120, 372, 1760
Alw26I	GTCTC	4 cut(s)	825, 1130, 1545, 2095
AlwI	GGATC	7 cut(s)	303, 752, 903, 1514, 2102, 2165, 2178
AlwNI	CAGNNNCTG	1 cut(s)	1310
Ama87I	CYCGRG	1 cut(s)	75
Aor13HI	TCCGGA	1 cut(s)	1095
ApaI	GGGCCC	2 cut(s)	910, 1927
ApeKI	GCWGC	1 cut(s)	1978
ApoI	RAATTY	5 cut(s)	564, 741, 1089, 1723, 2214
Asp700I	GAANNNNTTC	3 cut(s)	336, 744, 777
Asp718I	GGTACC	1 cut(s)	1066
AspS9I	GGNCC	6 cut(s)	906, 907, 1909, 1923, 1924, 2063
AsuC2I	CCSGG	2 cut(s)	1922, 2203
AsuHPI	GGTGA	5 cut(s)	139, 707, 1396, 1946, 2108
AvaI	CYCGRG	1 cut(s)	75
BaeGI	GKGCMC	2 cut(s)	910, 1927
BalI	TGGCCA	3 cut(s)	540, 840, 1890
BamHI	GGATCC	1 cut(s)	2170
BanI	GGYRCC	1 cut(s)	1066
BanII	GRGCYC	3 cut(s)	120, 910, 1927
BarI	GAAGNNNNNNTAC	2 cut(s)	1283, 1315
BbsI	GAAGAC	1 cut(s)	1298
Bbv12I	GWGCWC	3 cut(s)	120, 372, 1760
BbvI	GCAGC	1 cut(s)	1990
BccI	CCATC	6 cut(s)	338, 530, 715, 1211, 1893, 2266
BceAI	ACGGC	1 cut(s)	844
BcgI	CGANNNNNNTGC	2 cut(s)	1358, 1392
BciT130I	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
BciVI	GTATCC	1 cut(s)	81
BclI	TGATCA	2 cut(s)	1197, 2122
BcnI	CCSGG	2 cut(s)	1922, 2203
BcoDI	GTCTC	4 cut(s)	825, 1130, 1545, 2095
BcuI	ACTAGT	1 cut(s)	2051
BfaI	CTAG	4 cut(s)	1253, 1821, 2052, 2175
BfmI	CTRYAG	5 cut(s)	92, 185, 702, 1178, 1210
BfuAI	ACCTGC	1 cut(s)	868
BfuI	GTATCC	1 cut(s)	81
BglII	AGATCT	1 cut(s)	1192
BisI	GCNGC	4 cut(s)	30, 1170, 1908, 1979
BlpI	GCTNAGC	2 cut(s)	1173, 1638
BlsI	GCNGC	4 cut(s)	31, 1171, 1909, 1980
Bme1390I	CCNGG	7 cut(s)	64, 249, 911, 1482, 1545, 1922, 2203
BmeT110I	CYCGRG	1 cut(s)	75
BmgT120I	GGNCC	6 cut(s)	906, 907, 1909, 1923, 1924, 2063
BmiI	GGNNCC	9 cut(s)	387, 501, 753, 908, 1068, 1925, 2099, 2172, 2200
BmrFI	CCNGG	7 cut(s)	64, 249, 911, 1482, 1545, 1922, 2203
BmsI	GCATC	7 cut(s)	540, 736, 912, 985, 1141, 1163, 1845
BoxI	GACNNNNGTC	1 cut(s)	712
BpiI	GAAGAC	1 cut(s)	1298
BpmI	CTGGAG	5 cut(s)	46, 738, 932, 1527, 2217
Bpu1102I	GCTNAGC	2 cut(s)	1173, 1638
BpuEI	CTTGAG	1 cut(s)	1336
BpuMI	CCSGG	2 cut(s)	1922, 2203
Bsa29I	ATCGAT	1 cut(s)	1675
BsaBI	GATNNNNATC	2 cut(s)	1221, 2112
BsaHI	GRCGYC	1 cut(s)	1968
BsaI	GGTCTC	1 cut(s)	1130
BsaJI	CCNNGG	5 cut(s)	407, 600, 909, 1481, 1698
BsaWI	WCCGGW	2 cut(s)	36, 1095
BsaXI	ACNNNNNCTCC	2 cut(s)	1793, 1823
Bsc4I	CCNNNNNNNGG	4 cut(s)	36, 248, 869, 1024
Bse1I	ACTGG	5 cut(s)	897, 954, 1407, 1735, 1891
Bse3DI	GCAATG	6 cut(s)	361, 552, 1272, 1374, 1386, 1950
Bse8I	GATNNNNATC	2 cut(s)	1221, 2112
BseAI	TCCGGA	1 cut(s)	1095
BseBI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
BseCI	ATCGAT	1 cut(s)	1675
BseDI	CCNNGG	5 cut(s)	407, 600, 909, 1481, 1698
BseGI	GGATG	6 cut(s)	145, 349, 425, 717, 958, 1156
BseJI	GATNNNNATC	2 cut(s)	1221, 2112
BseLI	CCNNNNNNNGG	4 cut(s)	36, 248, 869, 1024
BseMI	GCAATG	6 cut(s)	361, 552, 1272, 1374, 1386, 1950
BseMII	CTCAG	7 cut(s)	293, 301, 639, 1164, 1629, 2061, 2106
BseNI	ACTGG	5 cut(s)	897, 954, 1407, 1735, 1891
BseSI	GKGCMC	2 cut(s)	910, 1927
BseXI	GCAGC	1 cut(s)	1990
BsgI	GTGCAG	2 cut(s)	177, 1997
Bsh1285I	CGRYCG	1 cut(s)	562
BshNI	GGYRCC	1 cut(s)	1066
BshVI	ATCGAT	1 cut(s)	1675
BsiEI	CGRYCG	1 cut(s)	562
BsiHKAI	GWGCWC	3 cut(s)	120, 372, 1760
BsiHKCI	CYCGRG	1 cut(s)	75
BsiSI	CCGG	5 cut(s)	37, 863, 1096, 1921, 2202
BsiWI	CGTACG	1 cut(s)	521
BslI	CCNNNNNNNGG	4 cut(s)	36, 248, 869, 1024
BsmAI	GTCTC	4 cut(s)	825, 1130, 1545, 2095
Bso31I	GGTCTC	1 cut(s)	1130
BsoBI	CYCGRG	1 cut(s)	75
Bsp120I	GGGCCC	2 cut(s)	906, 1923
Bsp1286I	GDGCHC	5 cut(s)	120, 372, 910, 1760, 1927
Bsp13I	TCCGGA	1 cut(s)	1095
Bsp1720I	GCTNAGC	2 cut(s)	1173, 1638
Bsp19I	CCATGG	1 cut(s)	1698
BspACI	CCGC	8 cut(s)	4, 30, 904, 1063, 1170, 1427, 1907, 1945
BspCNI	CTCAG	7 cut(s)	294, 300, 640, 1165, 1630, 2062, 2105
BspDI	ATCGAT	1 cut(s)	1675
BspEI	TCCGGA	1 cut(s)	1095
BspLI	GGNNCC	9 cut(s)	387, 501, 753, 908, 1068, 1925, 2099, 2172, 2200
BspMI	ACCTGC	1 cut(s)	868
BspPI	GGATC	7 cut(s)	303, 752, 903, 1514, 2102, 2165, 2178
BspQI	GCTCTTC	1 cut(s)	240
BspT107I	GGYRCC	1 cut(s)	1066
BspTNI	GGTCTC	1 cut(s)	1130
BsrDI	GCAATG	6 cut(s)	361, 552, 1272, 1374, 1386, 1950
BsrI	ACTGG	5 cut(s)	897, 954, 1407, 1735, 1891
BssECI	CCNNGG	5 cut(s)	407, 600, 909, 1481, 1698
BssNI	GRCGYC	1 cut(s)	1968
BssT1I	CCWWGG	2 cut(s)	600, 1698
Bst2UI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
Bst4CI	ACNGT	9 cut(s)	189, 458, 703, 1214, 1266, 1452, 1690, 1777, 2011
Bst6I	CTCTTC	6 cut(s)	60, 129, 240, 510, 1482, 1836
BstACI	GRCGYC	1 cut(s)	1968
BstAPI	GCANNNNNTGC	1 cut(s)	1151
BstC8I	GCNNGC	3 cut(s)	27, 906, 1983
BstDSI	CCRYGG	1 cut(s)	1698
BstF5I	GGATG	6 cut(s)	145, 349, 425, 717, 958, 1156
BstMAI	GTCTC	4 cut(s)	825, 1130, 1545, 2095
BstMCI	CGRYCG	1 cut(s)	562
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 200, 837, 1151
BstNI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
BstNSI	RCATGY	1 cut(s)	1014
BstPAI	GACNNNNGTC	1 cut(s)	712
BstSCI	CCNGG	7 cut(s)	62, 247, 909, 1480, 1543, 1920, 2201
BstSFI	CTRYAG	5 cut(s)	92, 185, 702, 1178, 1210
BstSLI	GKGCMC	2 cut(s)	910, 1927
BstV1I	GCAGC	1 cut(s)	1990
BstV2I	GAAGAC	1 cut(s)	1298
BstX2I	RGATCY	3 cut(s)	757, 1192, 2170
BstYI	RGATCY	3 cut(s)	757, 1192, 2170
Bsu15I	ATCGAT	1 cut(s)	1675
BsuI	GTATCC	1 cut(s)	81
BsuTUI	ATCGAT	1 cut(s)	1675
BtgI	CCRYGG	1 cut(s)	1698
BtsCI	GGATG	6 cut(s)	145, 349, 425, 717, 958, 1156
BtsI	GCAGTG	2 cut(s)	1362, 1601
BtsIMutI	CAGTG	8 cut(s)	295, 699, 1219, 1271, 1362, 1601, 2007, 2165
BveI	ACCTGC	1 cut(s)	868
Cac8I	GCNNGC	3 cut(s)	27, 906, 1983
CaiI	CAGNNNCTG	1 cut(s)	1310
Cfr13I	GGNCC	6 cut(s)	906, 907, 1909, 1923, 1924, 2063
ClaI	ATCGAT	1 cut(s)	1675
CseI	GACGC	1 cut(s)	1957
CsiI	ACCWGGT	1 cut(s)	247
Csp6I	GTAC	7 cut(s)	522, 1041, 1067, 1300, 1342, 1410, 1942
CviQI	GTAC	7 cut(s)	522, 1041, 1067, 1300, 1342, 1410, 1942
DraIII	CACNNNGTG	1 cut(s)	1266
EaeI	YGGCCR	5 cut(s)	410, 538, 838, 1888, 2239
Eam1104I	CTCTTC	6 cut(s)	60, 129, 240, 510, 1482, 1836
EarI	CTCTTC	6 cut(s)	60, 129, 240, 510, 1482, 1836
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	2 cut(s)	600, 1698
Eco24I	GRGCYC	3 cut(s)	120, 910, 1927
Eco31I	GGTCTC	1 cut(s)	1130
Eco32I	GATATC	1 cut(s)	2193
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	4 cut(s)	157, 569, 731, 1057
Eco88I	CYCGRG	1 cut(s)	75
EcoICRI	GAGCTC	1 cut(s)	118
EcoO109I	RGGNCCY	1 cut(s)	907
EcoRI	GAATTC	2 cut(s)	564, 1089
EcoRII	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
EcoRV	GATATC	1 cut(s)	2193
EcoT14I	CCWWGG	2 cut(s)	600, 1698
EcoT22I	ATGCAT	3 cut(s)	1156, 1285, 1860
EcoT38I	GRGCYC	3 cut(s)	120, 910, 1927
ErhI	CCWWGG	2 cut(s)	600, 1698
FalI	AAGNNNNNCTT	4 cut(s)	321, 353, 1865, 1897
FauI	CCCGC	3 cut(s)	897, 1056, 1420
FbaI	TGATCA	2 cut(s)	1197, 2122
Fnu4HI	GCNGC	4 cut(s)	30, 1170, 1908, 1979
FokI	GGATG	6 cut(s)	152, 356, 412, 724, 965, 1163
FriOI	GRGCYC	3 cut(s)	120, 910, 1927
Fsp4HI	GCNGC	4 cut(s)	30, 1170, 1908, 1979
FspBI	CTAG	4 cut(s)	1253, 1821, 2052, 2175
GluI	GCNGC	4 cut(s)	30, 1170, 1908, 1979
GsuI	CTGGAG	5 cut(s)	46, 738, 932, 1527, 2217
HapII	CCGG	5 cut(s)	37, 863, 1096, 1921, 2202
HgaI	GACGC	1 cut(s)	1957
Hin1I	GRCGYC	1 cut(s)	1968
HincII	GTYRAC	1 cut(s)	2286
HindII	GTYRAC	1 cut(s)	2286
HindIII	AAGCTT	2 cut(s)	581, 1995
HinfI	GANTC	8 cut(s)	13, 790, 967, 1257, 1307, 1734, 1808, 2266
HpaII	CCGG	5 cut(s)	37, 863, 1096, 1921, 2202
HphI	GGTGA	5 cut(s)	139, 707, 1396, 1946, 2108
Hpy166II	GTNNAC	2 cut(s)	1501, 2286
Hpy188III	TCNNGA	8 cut(s)	17, 341, 509, 755, 1075, 1096, 1190, 2196
Hpy8I	GTNNAC	2 cut(s)	1501, 2286
HpyAV	CCTTC	5 cut(s)	181, 527, 771, 1081, 2045
HpyCH4III	ACNGT	9 cut(s)	189, 458, 703, 1214, 1266, 1452, 1690, 1777, 2011
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 200, 837, 1151
Hsp92I	GRCGYC	1 cut(s)	1968
Kpn2I	TCCGGA	1 cut(s)	1095
KpnI	GGTACC	1 cut(s)	1070
Ksp22I	TGATCA	2 cut(s)	1197, 2122
LguI	GCTCTTC	1 cut(s)	240
LmnI	GCTCC	5 cut(s)	385, 499, 757, 1547, 2198
Lsp1109I	GCAGC	1 cut(s)	1990
LweI	GCATC	7 cut(s)	540, 736, 912, 985, 1141, 1163, 1845
MabI	ACCWGGT	1 cut(s)	247
MaeI	CTAG	4 cut(s)	1253, 1821, 2052, 2175
MaeIII	GTNAC	8 cut(s)	458, 482, 663, 695, 976, 1328, 1402, 1799
MfeI	CAATTG	2 cut(s)	204, 1614
MflI	RGATCY	3 cut(s)	757, 1192, 2170
MhlI	GDGCHC	5 cut(s)	120, 372, 910, 1760, 1927
MlsI	TGGCCA	3 cut(s)	540, 840, 1890
MluNI	TGGCCA	3 cut(s)	540, 840, 1890
MlyI	GAGTC	3 cut(s)	1728, 1802, 2275
MmeI	TCCRAC	1 cut(s)	1426
Mox20I	TGGCCA	3 cut(s)	540, 840, 1890
Mph1103I	ATGCAT	3 cut(s)	1156, 1285, 1860
MroI	TCCGGA	1 cut(s)	1095
MroXI	GAANNNNTTC	3 cut(s)	336, 744, 777
MscI	TGGCCA	3 cut(s)	540, 840, 1890
MseI	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
MslI	CAYNNNNRTG	4 cut(s)	303, 531, 1379, 2012
Msp20I	TGGCCA	3 cut(s)	540, 840, 1890
MspA1I	CMGCKG	1 cut(s)	1172
MspI	CCGG	5 cut(s)	37, 863, 1096, 1921, 2202
MspR9I	CCNGG	7 cut(s)	64, 249, 911, 1482, 1545, 1922, 2203
MunI	CAATTG	2 cut(s)	204, 1614
MvaI	CCWGG	5 cut(s)	64, 249, 911, 1482, 1545
MwoI	GCNNNNNNNGC	4 cut(s)	174, 200, 837, 1151
NciI	CCSGG	2 cut(s)	1922, 2203
NcoI	CCATGG	1 cut(s)	1698
NlaIV	GGNNCC	9 cut(s)	387, 501, 753, 908, 1068, 1925, 2099, 2172, 2200
NmeAIII	GCCGAG	1 cut(s)	388
NmuCI	GTSAC	3 cut(s)	458, 695, 1402
NsiI	ATGCAT	3 cut(s)	1156, 1285, 1860
NspI	RCATGY	1 cut(s)	1014
OliI	CACNNNNGTG	1 cut(s)	2012
PaeR7I	CTCGAG	1 cut(s)	75
PciSI	GCTCTTC	1 cut(s)	240
PcsI	WCGNNNNNNNCGW	1 cut(s)	415
PdmI	GAANNNNTTC	3 cut(s)	336, 744, 777
PfeI	GAWTC	5 cut(s)	13, 790, 967, 1257, 1307
Pfl23II	CGTACG	1 cut(s)	521
PflMI	CCANNNNNTGG	2 cut(s)	248, 1024
PfoI	TCCNGGA	1 cut(s)	1543
PkrI	GCNGC	4 cut(s)	31, 1171, 1909, 1980
Ple19I	CGATCG	1 cut(s)	562
PleI	GAGTC	3 cut(s)	1728, 1802, 2274
PpsI	GAGTC	3 cut(s)	1728, 1802, 2274
PshAI	GACNNNNGTC	1 cut(s)	712
PsiI	TTATAA	1 cut(s)	1248
Psp124BI	GAGCTC	1 cut(s)	120
Psp6I	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
PspGI	CCWGG	5 cut(s)	62, 247, 909, 1480, 1543
PspLI	CGTACG	1 cut(s)	521
PspN4I	GGNNCC	9 cut(s)	387, 501, 753, 908, 1068, 1925, 2099, 2172, 2200
PspOMI	GGGCCC	2 cut(s)	906, 1923
PspPI	GGNCC	6 cut(s)	906, 907, 1909, 1923, 1924, 2063
PspXI	VCTCGAGB	1 cut(s)	75
PsrI	GAACNNNNNNTAC	2 cut(s)	308, 340
PstNI	CAGNNNCTG	1 cut(s)	1310
PsuI	RGATCY	3 cut(s)	757, 1192, 2170
PvuI	CGATCG	1 cut(s)	562
RsaI	GTAC	7 cut(s)	523, 1042, 1068, 1301, 1343, 1411, 1943
RsaNI	GTAC	7 cut(s)	522, 1041, 1067, 1300, 1342, 1410, 1942
RseI	CAYNNNNRTG	4 cut(s)	303, 531, 1379, 2012
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	240
SaqAI	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
SatI	GCNGC	4 cut(s)	30, 1170, 1908, 1979
Sau96I	GGNCC	6 cut(s)	906, 907, 1909, 1923, 1924, 2063
SchI	GAGTC	3 cut(s)	1728, 1802, 2275
ScrFI	CCNGG	7 cut(s)	64, 249, 911, 1482, 1545, 1922, 2203
SduI	GDGCHC	5 cut(s)	120, 372, 910, 1760, 1927
SexAI	ACCWGGT	1 cut(s)	247
SfaNI	GCATC	7 cut(s)	540, 736, 912, 985, 1141, 1163, 1845
SfcI	CTRYAG	5 cut(s)	92, 185, 702, 1178, 1210
Sfr274I	CTCGAG	1 cut(s)	75
SlaI	CTCGAG	1 cut(s)	75
SmiMI	CAYNNNNRTG	4 cut(s)	303, 531, 1379, 2012
SmlI	CTYRAG	2 cut(s)	75, 1351
SmoI	CTYRAG	2 cut(s)	75, 1351
SpeI	ACTAGT	1 cut(s)	2051
SsiI	CCGC	8 cut(s)	4, 30, 904, 1063, 1170, 1427, 1907, 1945
SspI	AATATT	1 cut(s)	1534
SspMI	CTAG	4 cut(s)	1253, 1821, 2052, 2175
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	7 cut(s)	62, 247, 909, 1480, 1543, 1920, 2201
StyI	CCWWGG	2 cut(s)	600, 1698
TaaI	ACNGT	9 cut(s)	189, 458, 703, 1214, 1266, 1452, 1690, 1777, 2011
TatI	WGTACW	1 cut(s)	1409
TauI	GCSGC	3 cut(s)	32, 1172, 1910
TfiI	GAWTC	5 cut(s)	13, 790, 967, 1257, 1307
Tru1I	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
Tru9I	TTAA	6 cut(s)	393, 453, 1530, 1727, 1869, 2208
TscAI	CASTG	8 cut(s)	295, 706, 1219, 1271, 1369, 1608, 2014, 2165
TseFI	GTSAC	3 cut(s)	458, 695, 1402
TseI	GCWGC	1 cut(s)	1978
Tsp45I	GTSAC	3 cut(s)	458, 695, 1402
TspDTI	ATGAA	8 cut(s)	164, 297, 411, 1387, 1397, 1560, 1931, 2235
TspGWI	ACGGA	3 cut(s)	257, 470, 724
TspRI	CASTG	8 cut(s)	295, 706, 1219, 1271, 1369, 1608, 2014, 2165
Van91I	CCANNNNNTGG	2 cut(s)	248, 1024
XapI	RAATTY	5 cut(s)	564, 741, 1089, 1723, 2214
XceI	RCATGY	1 cut(s)	1014
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1442
XhoI	CTCGAG	1 cut(s)	75
XmnI	GAANNNNTTC	3 cut(s)	336, 744, 777
XspI	CTAG	4 cut(s)	1253, 1821, 2052, 2175
Zsp2I	ATGCAT	3 cut(s)	1156, 1285, 1860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.