Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003721

Physical Location & Seq

Genomic Coordinates Forward (+)

13549687 .. 13554667

Gene Structure 4981 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1164000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGAGCTTGTTTCTCTTTTCTCCAGGTGCATCTCTCTCACATTGCATTTCAAATGGAGGGTTCTTTCTAAAAATTAAAAATTTGTTGTGGGCTTTGTTTTTGAGGTTGGAATTGATGTGGGTTTCTGAGAAAGTTGAGATTTTTGTTATGTTTGGAAGGTATGTTGCTCGAGGAAATGGGATTCTACAACCCCATCAAATGATAAGCGAGCTCGAAAACGTGATCATGGACGATGAAGGCATGCAGAAGCTCAAAGATAGCTCCTTCAGCAAGGTCTTACAGTCTGCCCAGGAAGCAATTGTTCTTGCTCCTTTCGTGGCTCTTGCACTTCGTCCAAGACCTGGTGTTTGGGAGTATGTTCGAGTTAATGTGTATGAACTCAGTGTGGATCATTTGAGTGTTGCAGAATATCTTCGGTTCAAGGAAGAGCTTATTGACGGAGAGTGCAACGACAAGTATGTGCTTGAACTTGATCTGGAGCCATTTAATGCATCATTCCCTCGACCAACCCGTTCGTCATCAATTGGAAATGGGGTTCAATTCCTTAACCGTCATTTATCTTCAAATATGTTCCGTAACAAAGAAAGTTTGGAGCCTCTTCTCGACTTTCTTCGTACGCACAAACATGATGGTCATGCAATGATGCTAAATGATCGGATTCAGAGCATACCCAGACTTCAGTCTGCTTTAGCAAAGGCAGAGGAATATCTTTCTAAGTTCCCATCAACTACACCATATTCTGAGTTTGAATTTGACTTACAAGGAATGGGATTTGAGAGAGGGTGGGGTGACACAGCACAACGGGTGTCAGAGATGGTGCATCTTCTCCTGGAAATTCTTCAGGCTCCTGATCCCTCTACCCTGGAAAACTTTCTTGGGAGGATCCCTATGGTGTTCAATGTTGTCATAGTGTCTCCACACGGCTACTTTGGCCAAGCTAACGTCTTGGGTTTGCCTGACACTGGAGGGCAGGTTGTTTATATACTGGACCAAGTGCGTGCCTTGGAGAGTGAGATGCTTCTTAGAATACAAAACCAAGGATTGGATGTTATTCCAAAAATTCTGATTGTTACCCGACTGATACCTGATGCAAAAAGGACAACATGCAACCAAAGATTGGAAAGAGTCAGTGGTACAGAACACACACATATCTTGCGGGTACCTTTCAGGACTGAGAATGGAATTCTGCGGAAATGGATTTCAAGATTTGATGTGTGGCCTTACTTGGAGAACTTTGCAGAAAGTCAAAAGGATAGGACTTTTCATTTTCATTTTTGCCATAACTTTATGATGCACACTGATGCCTCGAATGAAATTGCTGCTGAGTTGCAGGGTGTTCCAGATCTAATCATTGGAAACTACAGTGATGGAAATCTTGTTGCAACTCTGCTATCTTATAAACTGGGAATCACACAGTGCAACATTGCTCATGCATTGGAGAAAACAAAGTACCCAGATTCTGATATATTTTGGAAGAAGCATGAAGATAAGTACCACTTTTCAAGTCAATTCACAGCCGATCTCATTGCAATGAACAATGCAGATTTCATAATCACCAGTACATACCAAGAGATTGCCGGAAGCAAGAATAACGTTGGACAGTATGAGAGCCACACTGCCTTCACTCTTCCTGGGCTGTATCGAGTTGTTCACGGGATTGATGTTTTTGATCCCAAGTTTAATATTGTCTCCCCGGGAGCAGATATGTGTATATACTTTCCATATTCTGACAAGGACAAAAGGCTTACTGCTCTACATGGGTCCATTGAAGAACTCTTATATGGTGCTGAGCAGAATGACGAGCATATTGGGCTGTTGAGTGATCGATCAAAGCCTATTGTCTTTTCCATGGCAAGACTTGATAGAGTGAAAAATTTAACCGGACTTGTTGAGTGCTATGCTAAGAGCACCAAGCTAAGAGAAATGGTAAACCTTGTTGTGGTTGGTGGTTACATGGATGTCAAGAACTCTAGGGATAGAGAAGAAATTACAGAGATTGAAAAGATGCATGACCTCATTAAGAACTACAACTTGAGTGGCCAGTTCCGATGGATAGCAGCACAGATGAACCGTGCTCGTAATGGTGAGCTCTACCGCTACATTGCAGACACAAAAGGCATCTTTGTGCAGCCTGCTTTCTATGAAGCTTTTGGCCTCACCGTGGTGGAAGCCATGACTTGTGGCCTTCCTACTTTTGCAACTTGTCATGGTGGCCCCGCTGAGATCATTGAGCACGGTACCTCAGGGTTCCATGTTGATCCCTATAACCCTGACCAAGTGGCTGAACTCCTGACTGACTTCTTTGATCAGTGCCAGAAGGATCCTGGCTACTGGGAAAAGATATCTCAAGCAGGGCTTAAACGAATTTATGAAAGGTACACTTGGAAGATATATTCAGAGAGGCTGCTCACATTAGCTGGGGTTTATGGCTTCTGGAAGCATGCGTCTAAGCTTGAGAGGCGAGAGACAAGGCGATATCTGGAGATGTTTTACACTCTCAAGTACCGGAATCTGGTGAAGTCCATTCCGCTGGCTGTTGATGAGCAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0006073 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009311 GO:0009505 GO:0009628 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016020 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0035251 GO:0036293 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070482 GO:0071695 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

851

Amino Acids

97.38

Weight (kDa)

5.99

Isoelectric Point (pI)

35.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	42 - 142	1.9e-39	Sucrose synthase N-terminal
SUS_EPBD	PF24862	177 - 264	1.9e-35	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	287 - 594	8.1e-162	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	604 - 770	4.5e-31	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	612 - 766	4.4e-15	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49190 AT5G49190 AT5G49190
fragaria_vesca	FvH4_4g18710
malus_domestica	MD13G1164000.v1.1 MD13G1164100.v1.1 MD13G1164200.v1.1 MD16G1164000.v1.1
prunus_persica	Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1 Prupe.1G131700_v2.0.a1
pyrus_communis	pycom13g14010 pycom16g13860 pycom16g13870
rosa_chinensis	RchiOBHm_Chr4g0423411
rosa_laevigata	RLG00000007559
rosa_multiflora	Rmu_sc0000893.1_g000010 Rmu_ssc0000174.1_g000013
rosa_roxburghii	Rroxscaffold_4G00291020 Rroxscaffold_5G00365630
rosa_rugosa	Rorug04G0187400 Rorug04G0187500 Rorug04G0187600 Rorug04G0187700 Rorug07G0218200
rosa_samantha	Rh3BG353800 Rh4AG246400 Rh4BG251800 Rh4CG260300 Rh4DG247200 Rh6BG052100
rosa_wichuraiana	Rw1G026460 Rw4G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1398
Acc36I	ACCTGC	1 cut(s)	961
Acc65I	GGTACC	2 cut(s)	1159, 2237
AccB1I	GGYRCC	2 cut(s)	1159, 2237
AccB7I	CCANNNNNTGG	3 cut(s)	341, 1042, 1117
AciI	CCGC	5 cut(s)	1156, 1189, 2095, 2217, 2528
AclI	AACGTT	1 cut(s)	1593
AclWI	GGATC	8 cut(s)	396, 845, 877, 890, 1664, 2252, 2315, 2328
AcoI	YGGCCR	2 cut(s)	931, 2038
AcsI	RAATTY	7 cut(s)	79, 749, 834, 1059, 1182, 1873, 2364
AcuI	CTGAAG	3 cut(s)	250, 662, 824
AdeI	CACNNNGTG	1 cut(s)	1416
AfaI	GTAC	9 cut(s)	616, 1135, 1161, 1451, 1493, 1561, 2239, 2378, 2504
AfiI	CCNNNNNNNGG	7 cut(s)	341, 962, 1042, 1117, 1939, 2161, 2512
AjnI	CCWGG	7 cut(s)	22, 288, 340, 828, 861, 1630, 2323
AjuI	GAANNNNNNNTTGG	2 cut(s)	858, 890
AleI	CACNNNNGTG	1 cut(s)	2162
AloI	GAACNNNNNNTCC	2 cut(s)	519, 551
Alw21I	GWGCWC	5 cut(s)	213, 1910, 2077, 2091, 2235
Alw26I	GTCTC	3 cut(s)	918, 1693, 2459
AlwI	GGATC	8 cut(s)	396, 845, 877, 890, 1664, 2252, 2315, 2328
AlwNI	CAGNNNCTG	1 cut(s)	1460
Ama87I	CYCGRG	2 cut(s)	168, 1693
AoxI	GGCC	6 cut(s)	931, 1217, 2038, 2152, 2182, 2212
ApeKI	GCWGC	5 cut(s)	1319, 2057, 2128, 2404, 2545
ApoI	RAATTY	7 cut(s)	79, 749, 834, 1059, 1182, 1873, 2364
ArsI	GACNNNNNNTTYG	2 cut(s)	330, 362
Asp700I	GAANNNNTTC	3 cut(s)	411, 837, 870
Asp718I	GGTACC	2 cut(s)	1159, 2237
AspS9I	GGNCC	3 cut(s)	988, 1761, 2213
AsuC2I	CCSGG	2 cut(s)	1694, 1695
AsuHPI	GGTGA	5 cut(s)	800, 1546, 2096, 2149, 2527
AvaI	CYCGRG	2 cut(s)	168, 1693
AvaII	GGWCC	2 cut(s)	988, 1761
AxyI	CCTNAGG	1 cut(s)	2242
BalI	TGGCCA	2 cut(s)	933, 2040
BamHI	GGATCC	2 cut(s)	882, 2320
BanI	GGYRCC	2 cut(s)	1159, 2237
BanII	GRGCYC	2 cut(s)	213, 2091
Bbv12I	GWGCWC	5 cut(s)	213, 1910, 2077, 2091, 2235
BbvI	GCAGC	4 cut(s)	1306, 2069, 2140, 2391
BccI	CCATC	6 cut(s)	201, 623, 730, 808, 1361, 2043
BceAI	ACGGC	1 cut(s)	937
BcgI	CGANNNNNNTGC	2 cut(s)	1508, 1542
BciT130I	CCWGG	7 cut(s)	24, 290, 342, 830, 863, 1632, 2325
BclI	TGATCA	2 cut(s)	222, 2305
BcnI	CCSGG	2 cut(s)	1694, 1695
BcoDI	GTCTC	3 cut(s)	918, 1693, 2459
BfaI	CTAG	1 cut(s)	1971
BfmI	CTRYAG	1 cut(s)	1360
BfuAI	ACCTGC	1 cut(s)	961
BglII	AGATCT	1 cut(s)	1342
BisI	GCNGC	5 cut(s)	1320, 2058, 2129, 2405, 2546
BlpI	GCTNAGC	1 cut(s)	1788
BlsI	GCNGC	5 cut(s)	1321, 2059, 2130, 2406, 2547
Bme1390I	CCNGG	9 cut(s)	24, 290, 342, 830, 863, 1632, 1694, 1695, 2325
Bme18I	GGWCC	2 cut(s)	988, 1761
BmeT110I	CYCGRG	2 cut(s)	168, 1693
BmgT120I	GGNCC	3 cut(s)	988, 1761, 2213
BmrFI	CCNGG	9 cut(s)	24, 290, 342, 830, 863, 1632, 1694, 1695, 2325
BmrI	ACTGGG	2 cut(s)	1412, 2341
BmuI	ACTGGG	2 cut(s)	1412, 2341
BoxI	GACNNNNGTC	1 cut(s)	679
BpmI	CTGGAG	4 cut(s)	6, 497, 984, 2501
Bpu1102I	GCTNAGC	1 cut(s)	1788
BpuEI	CTTGAG	4 cut(s)	2053, 2331, 2474, 2483
BpuMI	CCSGG	2 cut(s)	1694, 1695
Bsa29I	ATCGAT	1 cut(s)	1825
BsaBI	GATNNNNATC	1 cut(s)	1371
BsaJI	CCNNGG	9 cut(s)	288, 861, 1002, 1036, 1631, 1692, 1693, 1848, 2160
BsaWI	WCCGGW	2 cut(s)	1880, 2505
BsaXI	ACNNNNNCTCC	2 cut(s)	957, 987
Bsc4I	CCNNNNNNNGG	7 cut(s)	341, 962, 1042, 1117, 1939, 2161, 2512
Bse1I	ACTGG	6 cut(s)	967, 990, 1407, 1557, 2041, 2336
Bse21I	CCTNAGG	1 cut(s)	2242
Bse3DI	GCAATG	6 cut(s)	40, 645, 1422, 1524, 1536, 2100
Bse8I	GATNNNNATC	1 cut(s)	1371
BseBI	CCWGG	7 cut(s)	24, 290, 342, 830, 863, 1632, 2325
BseCI	ATCGAT	1 cut(s)	1825
BseDI	CCNNGG	9 cut(s)	288, 861, 1002, 1036, 1631, 1692, 1693, 1848, 2160
BseGI	GGATG	2 cut(s)	1051, 1963
BseJI	GATNNNNATC	1 cut(s)	1371
BseLI	CCNNNNNNNGG	7 cut(s)	341, 962, 1042, 1117, 1939, 2161, 2512
BseMI	GCAATG	6 cut(s)	40, 645, 1422, 1524, 1536, 2100
BseMII	CTCAG	8 cut(s)	117, 394, 732, 1164, 1314, 1779, 2211, 2256
BseNI	ACTGG	6 cut(s)	967, 990, 1407, 1557, 2041, 2336
BseXI	GCAGC	4 cut(s)	1306, 2069, 2140, 2391
BseYI	CCCAGC	1 cut(s)	2417
BsgI	GTGCAG	1 cut(s)	2147
BshFI	GGCC	6 cut(s)	933, 1219, 2040, 2154, 2184, 2214
BshNI	GGYRCC	2 cut(s)	1159, 2237
BshVI	ATCGAT	1 cut(s)	1825
BsiHKAI	GWGCWC	5 cut(s)	213, 1910, 2077, 2091, 2235
BsiHKCI	CYCGRG	2 cut(s)	168, 1693
BsiSI	CCGG	4 cut(s)	1578, 1694, 1881, 2506
BsiWI	CGTACG	1 cut(s)	614
BslI	CCNNNNNNNGG	7 cut(s)	341, 962, 1042, 1117, 1939, 2161, 2512
BsmAI	GTCTC	3 cut(s)	918, 1693, 2459
BsnI	GGCC	6 cut(s)	933, 1219, 2040, 2154, 2184, 2214
BsoBI	CYCGRG	2 cut(s)	168, 1693
Bsp1286I	GDGCHC	5 cut(s)	213, 1910, 2077, 2091, 2235
Bsp1720I	GCTNAGC	1 cut(s)	1788
Bsp19I	CCATGG	1 cut(s)	1848
BspACI	CCGC	5 cut(s)	1156, 1189, 2095, 2217, 2528
BspANI	GGCC	6 cut(s)	933, 1219, 2040, 2154, 2184, 2214
BspCNI	CTCAG	8 cut(s)	118, 393, 733, 1165, 1315, 1780, 2212, 2255
BspDI	ATCGAT	1 cut(s)	1825
BspMI	ACCTGC	1 cut(s)	961
BspPI	GGATC	8 cut(s)	396, 845, 877, 890, 1664, 2252, 2315, 2328
BspQI	GCTCTTC	1 cut(s)	420
BspT107I	GGYRCC	2 cut(s)	1159, 2237
BsrDI	GCAATG	6 cut(s)	40, 645, 1422, 1524, 1536, 2100
BsrI	ACTGG	6 cut(s)	967, 990, 1407, 1557, 2041, 2336
BssECI	CCNNGG	9 cut(s)	288, 861, 1002, 1036, 1631, 1692, 1693, 1848, 2160
BssT1I	CCWWGG	3 cut(s)	1002, 1036, 1848
Bst2UI	CCWGG	7 cut(s)	24, 290, 342, 830, 863, 1632, 2325
Bst4CI	ACNGT	8 cut(s)	282, 551, 1364, 1416, 1602, 2072, 2161, 2237
Bst6I	CTCTTC	3 cut(s)	420, 603, 1632
BstC8I	GCNNGC	6 cut(s)	209, 242, 999, 2133, 2442, 2532
BstDSI	CCRYGG	2 cut(s)	1848, 2160
BstF5I	GGATG	2 cut(s)	1051, 1963
BstMAI	GTCTC	3 cut(s)	918, 1693, 2459
BstMWI	GCNNNNNNNGC	5 cut(s)	267, 293, 930, 1810, 2458
BstNI	CCWGG	7 cut(s)	24, 290, 342, 830, 863, 1632, 2325
BstNSI	RCATGY	3 cut(s)	244, 1107, 2444
BstPAI	GACNNNNGTC	1 cut(s)	679
BstSCI	CCNGG	9 cut(s)	22, 288, 340, 828, 861, 1630, 1692, 1693, 2323
BstSFI	CTRYAG	1 cut(s)	1360
BstV1I	GCAGC	4 cut(s)	1306, 2069, 2140, 2391
BstX2I	RGATCY	3 cut(s)	882, 1342, 2320
BstXI	CCANNNNNNTGG	1 cut(s)	2530
BstYI	RGATCY	3 cut(s)	882, 1342, 2320
Bsu15I	ATCGAT	1 cut(s)	1825
Bsu36I	CCTNAGG	1 cut(s)	2242
BsuRI	GGCC	6 cut(s)	933, 1219, 2040, 2154, 2184, 2214
BsuTUI	ATCGAT	1 cut(s)	1825
BtgI	CCRYGG	2 cut(s)	1848, 2160
BtsCI	GGATG	2 cut(s)	1051, 1963
BtsI	GCAGTG	1 cut(s)	1614
BtsIMutI	CAGTG	8 cut(s)	388, 960, 1135, 1296, 1369, 1421, 1614, 2315
BveI	ACCTGC	1 cut(s)	961
Cac8I	GCNNGC	6 cut(s)	209, 242, 999, 2133, 2442, 2532
CaiI	CAGNNNCTG	1 cut(s)	1460
Cfr13I	GGNCC	3 cut(s)	988, 1761, 2213
Cfr9I	CCCGGG	1 cut(s)	1693
ClaI	ATCGAT	1 cut(s)	1825
CseI	GACGC	1 cut(s)	2433
CsiI	ACCWGGT	1 cut(s)	340
Csp6I	GTAC	9 cut(s)	615, 1134, 1160, 1450, 1492, 1560, 2238, 2377, 2503
CspCI	CAANNNNNGTGG	2 cut(s)	2017, 2052
CviQI	GTAC	9 cut(s)	615, 1134, 1160, 1450, 1492, 1560, 2238, 2377, 2503
DraIII	CACNNNGTG	1 cut(s)	1416
EaeI	YGGCCR	2 cut(s)	931, 2038
Eam1104I	CTCTTC	3 cut(s)	420, 603, 1632
EarI	CTCTTC	3 cut(s)	420, 603, 1632
Ecl136II	GAGCTC	2 cut(s)	211, 2089
Eco130I	CCWWGG	3 cut(s)	1002, 1036, 1848
Eco24I	GRGCYC	2 cut(s)	213, 2091
Eco32I	GATATC	2 cut(s)	2343, 2477
Eco47I	GGWCC	2 cut(s)	988, 1761
Eco53kI	GAGCTC	2 cut(s)	211, 2089
Eco57I	CTGAAG	3 cut(s)	250, 662, 824
Eco81I	CCTNAGG	1 cut(s)	2242
Eco88I	CYCGRG	2 cut(s)	168, 1693
EcoICRI	GAGCTC	2 cut(s)	211, 2089
EcoRI	GAATTC	1 cut(s)	1182
EcoRII	CCWGG	7 cut(s)	22, 288, 340, 828, 861, 1630, 2323
EcoRV	GATATC	2 cut(s)	2343, 2477
EcoT14I	CCWWGG	3 cut(s)	1002, 1036, 1848
EcoT22I	ATGCAT	3 cut(s)	493, 1435, 2010
EcoT38I	GRGCYC	2 cut(s)	213, 2091
ErhI	CCWWGG	3 cut(s)	1002, 1036, 1848
FalI	AAGNNNNNCTT	6 cut(s)	1481, 1513, 2340, 2372, 2365, 2397
FauI	CCCGC	2 cut(s)	1149, 2224
FbaI	TGATCA	2 cut(s)	222, 2305
Fnu4HI	GCNGC	5 cut(s)	1320, 2058, 2129, 2405, 2546
FokI	GGATG	2 cut(s)	1058, 1970
FriOI	GRGCYC	2 cut(s)	213, 2091
Fsp4HI	GCNGC	5 cut(s)	1320, 2058, 2129, 2405, 2546
FspBI	CTAG	1 cut(s)	1971
GluI	GCNGC	5 cut(s)	1320, 2058, 2129, 2405, 2546
GsaI	CCCAGC	1 cut(s)	2421
GsuI	CTGGAG	4 cut(s)	6, 497, 984, 2501
HaeIII	GGCC	6 cut(s)	933, 1219, 2040, 2154, 2184, 2214
HapII	CCGG	4 cut(s)	1578, 1694, 1881, 2506
HgaI	GACGC	1 cut(s)	2433
HindIII	AAGCTT	2 cut(s)	2145, 2450
HinfI	GANTC	6 cut(s)	181, 658, 1125, 1407, 1457, 2509
HpaII	CCGG	4 cut(s)	1578, 1694, 1881, 2506
HphI	GGTGA	5 cut(s)	800, 1546, 2096, 2149, 2527
Hpy166II	GTNNAC	3 cut(s)	1651, 1930, 2379
Hpy8I	GTNNAC	3 cut(s)	1651, 1930, 2379
HpyAV	CCTTC	6 cut(s)	150, 230, 274, 1630, 2195, 2311
HpyCH4III	ACNGT	8 cut(s)	282, 551, 1364, 1416, 1602, 2072, 2161, 2237
HpyCH4IV	ACGT	3 cut(s)	219, 942, 1593
HpyF10VI	GCNNNNNNNGC	5 cut(s)	267, 293, 930, 1810, 2458
HpySE526I	ACGT	3 cut(s)	219, 942, 1593
KpnI	GGTACC	2 cut(s)	1163, 2241
Ksp22I	TGATCA	2 cut(s)	222, 2305
LguI	GCTCTTC	1 cut(s)	420
LmnI	GCTCC	6 cut(s)	266, 313, 478, 592, 850, 1697
Lsp1109I	GCAGC	4 cut(s)	1306, 2069, 2140, 2391
MabI	ACCWGGT	1 cut(s)	340
MaeI	CTAG	1 cut(s)	1971
MaeII	ACGT	3 cut(s)	219, 942, 1593
MaeIII	GTNAC	4 cut(s)	575, 788, 1069, 1949
MfeI	CAATTG	2 cut(s)	297, 522
MflI	RGATCY	3 cut(s)	882, 1342, 2320
MhlI	GDGCHC	5 cut(s)	213, 1910, 2077, 2091, 2235
MlsI	TGGCCA	2 cut(s)	933, 2040
MluNI	TGGCCA	2 cut(s)	933, 2040
MlyI	GAGTC	1 cut(s)	1134
MmeI	TCCRAC	2 cut(s)	87, 1576
Mox20I	TGGCCA	2 cut(s)	933, 2040
Mph1103I	ATGCAT	3 cut(s)	493, 1435, 2010
MroXI	GAANNNNTTC	3 cut(s)	411, 837, 870
MscI	TGGCCA	2 cut(s)	933, 2040
MseI	TTAA	8 cut(s)	75, 366, 486, 546, 1680, 1877, 2019, 2358
MslI	CAYNNNNRTG	6 cut(s)	396, 624, 1299, 1529, 2123, 2162
Msp20I	TGGCCA	2 cut(s)	933, 2040
MspA1I	CMGCKG	2 cut(s)	2219, 2530
MspI	CCGG	4 cut(s)	1578, 1694, 1881, 2506
MspR9I	CCNGG	9 cut(s)	24, 290, 342, 830, 863, 1632, 1694, 1695, 2325
MunI	CAATTG	2 cut(s)	297, 522
MvaI	CCWGG	7 cut(s)	24, 290, 342, 830, 863, 1632, 2325
MwoI	GCNNNNNNNGC	5 cut(s)	267, 293, 930, 1810, 2458
NciI	CCSGG	2 cut(s)	1694, 1695
NcoI	CCATGG	1 cut(s)	1848
NmuCI	GTSAC	1 cut(s)	788
NsiI	ATGCAT	3 cut(s)	493, 1435, 2010
NspI	RCATGY	3 cut(s)	244, 1107, 2444
OliI	CACNNNNGTG	1 cut(s)	2162
PaeI	GCATGC	2 cut(s)	244, 2444
PaeR7I	CTCGAG	1 cut(s)	168
PciSI	GCTCTTC	1 cut(s)	420
PcsI	WCGNNNNNNNCGW	1 cut(s)	508
PdmI	GAANNNNTTC	3 cut(s)	411, 837, 870
PfeI	GAWTC	5 cut(s)	181, 658, 1407, 1457, 2509
Pfl23II	CGTACG	1 cut(s)	614
PflMI	CCANNNNNTGG	3 cut(s)	341, 1042, 1117
PfoI	TCCNGGA	1 cut(s)	828
PkrI	GCNGC	5 cut(s)	1321, 2059, 2130, 2406, 2547
PleI	GAGTC	1 cut(s)	1133
PpsI	GAGTC	1 cut(s)	1133
PshAI	GACNNNNGTC	1 cut(s)	679
PsiI	TTATAA	1 cut(s)	1398
Psp124BI	GAGCTC	2 cut(s)	213, 2091
Psp1406I	AACGTT	1 cut(s)	1593
Psp6I	CCWGG	7 cut(s)	22, 288, 340, 828, 861, 1630, 2323
PspFI	CCCAGC	1 cut(s)	2417
PspGI	CCWGG	7 cut(s)	22, 288, 340, 828, 861, 1630, 2323
PspLI	CGTACG	1 cut(s)	614
PspPI	GGNCC	3 cut(s)	988, 1761, 2213
PspXI	VCTCGAGB	1 cut(s)	168
PstNI	CAGNNNCTG	1 cut(s)	1460
PsuI	RGATCY	3 cut(s)	882, 1342, 2320
RsaI	GTAC	9 cut(s)	616, 1135, 1161, 1451, 1493, 1561, 2239, 2378, 2504
RsaNI	GTAC	9 cut(s)	615, 1134, 1160, 1450, 1492, 1560, 2238, 2377, 2503
RseI	CAYNNNNRTG	6 cut(s)	396, 624, 1299, 1529, 2123, 2162
SacI	GAGCTC	2 cut(s)	213, 2091
SapI	GCTCTTC	1 cut(s)	420
SaqAI	TTAA	8 cut(s)	75, 366, 486, 546, 1680, 1877, 2019, 2358
SatI	GCNGC	5 cut(s)	1320, 2058, 2129, 2405, 2546
Sau96I	GGNCC	3 cut(s)	988, 1761, 2213
SchI	GAGTC	1 cut(s)	1134
ScrFI	CCNGG	9 cut(s)	24, 290, 342, 830, 863, 1632, 1694, 1695, 2325
SduI	GDGCHC	5 cut(s)	213, 1910, 2077, 2091, 2235
SexAI	ACCWGGT	1 cut(s)	340
SfcI	CTRYAG	1 cut(s)	1360
Sfr274I	CTCGAG	1 cut(s)	168
SinI	GGWCC	2 cut(s)	988, 1761
SlaI	CTCGAG	1 cut(s)	168
SmaI	CCCGGG	1 cut(s)	1695
SmiMI	CAYNNNNRTG	6 cut(s)	396, 624, 1299, 1529, 2123, 2162
SmlI	CTYRAG	5 cut(s)	168, 2032, 2346, 2453, 2498
SmoI	CTYRAG	5 cut(s)	168, 2032, 2346, 2453, 2498
SphI	GCATGC	2 cut(s)	244, 2444
SsiI	CCGC	5 cut(s)	1156, 1189, 2095, 2217, 2528
SspI	AATATT	1 cut(s)	1684
SspMI	CTAG	1 cut(s)	1971
SstI	GAGCTC	2 cut(s)	213, 2091
StyD4I	CCNGG	9 cut(s)	22, 288, 340, 828, 861, 1630, 1692, 1693, 2323
StyI	CCWWGG	3 cut(s)	1002, 1036, 1848
TaaI	ACNGT	8 cut(s)	282, 551, 1364, 1416, 1602, 2072, 2161, 2237
TaiI	ACGT	3 cut(s)	222, 945, 1596
TaqI	TCGA	8 cut(s)	169, 213, 361, 502, 603, 1307, 1642, 1825
TatI	WGTACW	1 cut(s)	1559
TfiI	GAWTC	5 cut(s)	181, 658, 1407, 1457, 2509
Tru1I	TTAA	8 cut(s)	75, 366, 486, 546, 1680, 1877, 2019, 2358
Tru9I	TTAA	8 cut(s)	75, 366, 486, 546, 1680, 1877, 2019, 2358
TscAI	CASTG	8 cut(s)	388, 967, 1135, 1303, 1369, 1421, 1621, 2315
TseFI	GTSAC	1 cut(s)	788
TseI	GCWGC	5 cut(s)	1319, 2057, 2128, 2404, 2545
Tsp45I	GTSAC	1 cut(s)	788
TspGWI	ACGGA	2 cut(s)	453, 563
TspMI	CCCGGG	1 cut(s)	1693
TspRI	CASTG	8 cut(s)	388, 967, 1135, 1303, 1369, 1421, 1621, 2315
Van91I	CCANNNNNTGG	3 cut(s)	341, 1042, 1117
VpaK11BI	GGWCC	2 cut(s)	988, 1761
XapI	RAATTY	7 cut(s)	79, 749, 834, 1059, 1182, 1873, 2364
XceI	RCATGY	3 cut(s)	244, 1107, 2444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2321
XhoI	CTCGAG	1 cut(s)	168
XmaI	CCCGGG	1 cut(s)	1693
XmnI	GAANNNNTTC	3 cut(s)	411, 837, 870
XspI	CTAG	1 cut(s)	1971
Zsp2I	ATGCAT	3 cut(s)	493, 1435, 2010

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.