FvH4_4g19630

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003111
Physical Location & Seq
Forward (+)
23219706 .. 23222609
2904 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19630.t1

Sequence Viewer

Length: 699 bp
ATGGCTCGGATAAAGCCGCAGGCGCTGCTACTTCAGAGCAAGAAGAAGAAGGGGCCGACTCGAATCAGTGTCGCTACCATAGTTATGTGCAATCTTATAATCGCTTTGATCGCCTTGTCCTTGGTCGCCACTTACCGCTACTGGTCCAGAAGGTCACTGGACCATTCCGGGTCGGACTTTTCTGCTTCAGGGGAAATGGATGCGTTTGCCAAGAAGGATGATCTTCCTGGTTACGCTATTTTAAACACATCAAAAGGTTATATCACAGTGGAACTTTACAAGGATGGTTCTCCAGAGGTTGTGGATAGATTTCTTGACTTATGTCAAAAGGGACACTTCAAAGGGATGCTCTTCAGTCATGTCATTAAACACTACGTAATTAAGGGAGGAGATTCCCAAGGACTTGGAGTAGCTGAAGATTGGATATCAAAAGGGAAGCTTCGTAGTCAACTTGTGACAAGTCCAAAGCATGAAGCATTTATGCTTGGAACTACAAAGAATAAACTAGAGAACAAAGGATTTGAGCTTGTTATCACGACGGCACCTATACCTGATTTAAATGACAAGCTTATTGTGTTTGGACGTGTCGTCAAAGGAGAGGATGTGGTGCAGGAAATTGAAGAGGTTGATACAGATGAACATTATCGGCCTAAATCTCCAGTAGGGATCACCAGCGTGACTCTAAAACGCGAGATATAA

Protein Analysis

233

Amino Acids

25.77

Weight (kDa)

9.04

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 81 - 227 7.7e-30 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 541
AccII CGCG 1 cut(s) 690
AciI CCGC 2 cut(s) 17, 136
AclWI GGATC 1 cut(s) 674
AcuI CTGAAG 4 cut(s) 17, 171, 337, 435
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 2 cut(s) 340, 620
AhdI GACNNNNNGTC 1 cut(s) 587
AjiI CACGTC 1 cut(s) 584
AjnI CCWGG 1 cut(s) 226
AleI CACNNNNGTG 1 cut(s) 674
AluBI AGCT 4 cut(s) 413, 439, 526, 568
AluI AGCT 4 cut(s) 413, 439, 526, 568
AlwI GGATC 1 cut(s) 674
AlwNI CAGNNNCTG 1 cut(s) 25
AoxI GGCC 2 cut(s) 53, 647
ApeKI GCWGC 1 cut(s) 25
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 3 cut(s) 53, 144, 160
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 661
AvaII GGWCC 2 cut(s) 144, 160
BanI GGYRCC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 12
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 228
BcnI CCSGG 1 cut(s) 169
BfaI CTAG 1 cut(s) 506
BfoI RGCGCY 1 cut(s) 26
BisI GCNGC 2 cut(s) 17, 26
BlsI GCNGC 2 cut(s) 18, 27
Bme1390I CCNGG 2 cut(s) 169, 228
Bme18I GGWCC 2 cut(s) 144, 160
BmeRI GACNNNNNGTC 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 584
BmgT120I GGNCC 3 cut(s) 53, 144, 160
BmiI GGNNCC 2 cut(s) 54, 543
BmrFI CCNGG 2 cut(s) 169, 228
BmsI GCATC 2 cut(s) 190, 336
BoxI GACNNNNGTC 1 cut(s) 321
BpmI CTGGAG 2 cut(s) 276, 642
BpuMI CCSGG 1 cut(s) 169
BsaAI YACGTR 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 120, 397
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bse1I ACTGG 3 cut(s) 146, 162, 659
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 120, 397
BseGI GGATG 5 cut(s) 205, 223, 289, 351, 607
BseNI ACTGG 3 cut(s) 146, 162, 659
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 690
BshFI GGCC 2 cut(s) 55, 649
BshNI GGYRCC 1 cut(s) 541
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 345
BsnI GGCC 2 cut(s) 55, 649
Bsp143I GATC 3 cut(s) 108, 220, 666
BspACI CCGC 2 cut(s) 17, 136
BspANI GGCC 2 cut(s) 55, 649
BspFNI CGCG 1 cut(s) 690
BspLI GGNNCC 2 cut(s) 54, 543
BspPI GGATC 1 cut(s) 674
BspQI GCTCTTC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 541
BsrI ACTGG 3 cut(s) 146, 162, 659
BssECI CCNNGG 2 cut(s) 120, 397
BssMI GATC 3 cut(s) 108, 220, 666
BssT1I CCWWGG 2 cut(s) 120, 397
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 2 cut(s) 356, 615
BstAPI GCANNNNNTGC 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 21
BstF5I GGATG 5 cut(s) 205, 223, 289, 351, 607
BstFNI CGCG 1 cut(s) 690
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 3 cut(s) 111, 223, 669
BstMBI GATC 3 cut(s) 108, 220, 666
BstMWI GCNNNNNNNGC 3 cut(s) 22, 25, 110
BstNI CCWGG 1 cut(s) 228
BstPAI GACNNNNGTC 1 cut(s) 321
BstSCI CCNGG 2 cut(s) 167, 226
BstSNI TACGTA 1 cut(s) 376
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 1 cut(s) 12
BstXI CCANNNNNNTGG 1 cut(s) 404
BsuRI GGCC 2 cut(s) 55, 649
BtrI CACGTC 1 cut(s) 584
BtsCI GGATG 5 cut(s) 205, 223, 289, 351, 607
BtsIMutI CAGTG 3 cut(s) 73, 155, 273
Cac8I GCNNGC 1 cut(s) 21
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 53, 144, 160
CviAII CATG 2 cut(s) 359, 470
CviJI RGCY 8 cut(s) 5, 16, 55, 413, 439, 526, 568, 649
CviKI_1 RGCY 8 cut(s) 5, 16, 55, 413, 439, 526, 568, 649
DpnI GATC 3 cut(s) 110, 222, 668
DpnII GATC 3 cut(s) 108, 220, 666
DraI TTTAAA 2 cut(s) 243, 558
DriI GACNNNNNGTC 1 cut(s) 587
Eam1104I CTCTTC 2 cut(s) 356, 615
Eam1105I GACNNNNNGTC 1 cut(s) 587
EarI CTCTTC 2 cut(s) 356, 615
Eco105I TACGTA 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 120, 397
Eco32I GATATC 1 cut(s) 426
Eco47I GGWCC 2 cut(s) 144, 160
Eco57I CTGAAG 4 cut(s) 17, 171, 337, 435
EcoRII CCWGG 1 cut(s) 226
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 2 cut(s) 120, 397
ErhI CCWWGG 2 cut(s) 120, 397
FaeI CATG 2 cut(s) 362, 473
FalI AAGNNNNNCTT 4 cut(s) 320, 352, 423, 455
FaqI GGGAC 1 cut(s) 345
FatI CATG 2 cut(s) 358, 469
Fnu4HI GCNGC 2 cut(s) 17, 26
FokI GGATG 5 cut(s) 212, 230, 296, 358, 614
Fsp4HI GCNGC 2 cut(s) 17, 26
FspBI CTAG 1 cut(s) 506
GlaI GCGC 1 cut(s) 24
GluI GCNGC 2 cut(s) 17, 26
GsuI CTGGAG 2 cut(s) 276, 642
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 2 cut(s) 55, 649
HapII CCGG 1 cut(s) 168
HhaI GCGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 362, 473
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 1 cut(s) 449
HindII GTYRAC 1 cut(s) 449
HindIII AAGCTT 2 cut(s) 437, 566
HinfI GANTC 4 cut(s) 58, 63, 392, 679
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 3 cut(s) 9, 36, 175
Hpy188III TCNNGA 4 cut(s) 147, 293, 314, 535
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 43, 144, 208
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 2 cut(s) 375, 583
HpyCH4V TGCA 2 cut(s) 90, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 25, 110
HpySE526I ACGT 2 cut(s) 375, 583
Hsp92II CATG 2 cut(s) 362, 473
HspAI GCGC 1 cut(s) 23
Kzo9I GATC 3 cut(s) 108, 220, 666
LguI GCTCTTC 1 cut(s) 356
Lsp1109I GCAGC 1 cut(s) 12
LweI GCATC 2 cut(s) 190, 336
MaeI CTAG 1 cut(s) 506
MaeII ACGT 2 cut(s) 375, 583
MaeIII GTNAC 4 cut(s) 153, 230, 454, 676
MalI GATC 3 cut(s) 110, 222, 668
MboI GATC 3 cut(s) 108, 220, 666
MboII GAAGA 6 cut(s) 55, 58, 215, 343, 428, 632
MluCI AATT 2 cut(s) 378, 615
MlyI GAGTC 2 cut(s) 52, 673
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 289, 380, 592, 616
MseI TTAA 4 cut(s) 242, 366, 381, 557
MslI CAYNNNNRTG 2 cut(s) 83, 674
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 2 cut(s) 169, 228
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 3 cut(s) 22, 25, 110
NciI CCSGG 1 cut(s) 169
NdeII GATC 3 cut(s) 108, 220, 666
NlaIII CATG 2 cut(s) 362, 473
NlaIV GGNNCC 2 cut(s) 54, 543
NmuCI GTSAC 3 cut(s) 153, 454, 676
OliI CACNNNNGTG 1 cut(s) 674
PciSI GCTCTTC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 63, 392
PkrI GCNGC 2 cut(s) 18, 27
PleI GAGTC 2 cut(s) 52, 673
PpsI GAGTC 2 cut(s) 52, 673
Ppu21I YACGTR 1 cut(s) 376
PshAI GACNNNNGTC 1 cut(s) 321
PsiI TTATAA 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 2 cut(s) 54, 543
PspPI GGNCC 3 cut(s) 53, 144, 160
PstNI CAGNNNCTG 1 cut(s) 25
RseI CAYNNNNRTG 2 cut(s) 83, 674
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 4 cut(s) 242, 366, 381, 557
SatI GCNGC 2 cut(s) 17, 26
Sau3AI GATC 3 cut(s) 108, 220, 666
Sau96I GGNCC 3 cut(s) 53, 144, 160
SchI GAGTC 2 cut(s) 52, 673
ScrFI CCNGG 2 cut(s) 169, 228
SfaNI GCATC 2 cut(s) 190, 336
SinI GGWCC 2 cut(s) 144, 160
SmiI ATTTAAAT 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 83, 674
SnaBI TACGTA 1 cut(s) 376
Sse9I AATT 2 cut(s) 378, 615
SsiI CCGC 2 cut(s) 17, 136
SspMI CTAG 1 cut(s) 506
StyD4I CCNGG 2 cut(s) 167, 226
StyI CCWWGG 2 cut(s) 120, 397
SwaI ATTTAAAT 1 cut(s) 558
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 2 cut(s) 378, 586
TaqI TCGA 1 cut(s) 61
TasI AATT 2 cut(s) 378, 615
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 2 cut(s) 63, 392
Tru1I TTAA 4 cut(s) 242, 366, 381, 557
Tru9I TTAA 4 cut(s) 242, 366, 381, 557
TscAI CASTG 3 cut(s) 73, 162, 273
TseFI GTSAC 3 cut(s) 153, 454, 676
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 3 cut(s) 153, 454, 676
TspDTI ATGAA 2 cut(s) 486, 651
TspRI CASTG 3 cut(s) 73, 162, 273
VpaK11BI GGWCC 2 cut(s) 144, 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XspI CTAG 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.