Rorug04G0200300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003111
Physical Location & Seq
Reverse (-)
34804348 .. 34807428
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0200300.1

Sequence Viewer

Length: 1725 bp
ATGGCCAACTCCTACTACGAGTCTCCGGCCACCCAAACTCCACTACTACTCGACGACGACGTGGTGGAGGCCGCCGTTGACTACCAAGGACACCCGGTCCTCAGGTCCACCACAGGCGGTTGGCGTTCCGCCTCATTCATAATAGGTGTGGAGATTGCGGAGAGGGTCGCCTACTATGGTATTGCCTCCAACCTTATCTCCTATCTGACAGGGCCTCTGGGTCAGTCAACGGCCACGGCGGCTCAAAATATCAACGTTTGGGACGGAACGGCGTCGTTGCTCACTTTGTTGGGAGCGTTCTTGGCCGATTCTTACCTCGGACGCTACCGCACCATCGTTGTTGCTTCTCTCCTCTACATTTTGGGACTTGGCTTGTTGACCCTCTCGGCCTTGCTTCCTTTTCTAAGTGCTTCTGACTGTGAAACAACCAATCAGTCCACATCATGTTCTTCTCGGCTTCAAGAATTGTTATTCTTCTTCTCTATATATCTAGTAGCTGTGGGGCAAGGTGGACACAAGCCTTGCGTTCAGGCTTTTGGAGCTGATCAGTTTGATGGACAAGATCCAGAGGAATGCAAAGCCAAAAGCTCATTCTTCAATTGGTGGTATTTCGGTATATGTGCAGGTCCCTTGTGTACATTTTCCTTATTAGCCTACATACAGGACAACCTGAGCTGGGGTCTTGGTTTTGGAATTCCTTGTATTGTGATGGTCCTCGCATTGTCTATTTTCCTTCTTGGAACTAGAACTTACCGGTATAGCGTCAGAGGGAATGAGGAAAGTCCGTTTGTGAGAATTGGTAAGGTGTTTGTTGTATCATTAAGGAATTGGCGAACTTGTTCTTCAGCAATAGCTTCTGAAGAGGAATCTCGAGGAATTCTGCCTCACCAAAGTTCTGAACAATTCAAGTTCCTCAACAAAGCCTTGCTTGCTCCAGATGATTTGAAGGGAAATGGGAAGGTGTGTACCGTCGTCGAGGTTGAAGAAGCAAAAAGCATTCTTCGGCTATTTCCAATATGGGCTACATGCTTGGCATATGCTATTGTGTTTGCACAGTTTTCGACCTTCTTCACCAAGCAAGGTGCTACCATGAACAGAACAATCGTGCCAGGTTTTGACATACCACCAGCTTCCCTTCAGACTTTTATCAGCCTAGCAACTATCATCTTCATTCCCATTTATGACCGCATTTTTGTACCAATGACTAGAGCTTATACTAGGAAACCCTCTGGCATCACAATGCTACAGAGAATTGGAACAGGGATGTGTTTATGTGCTATTTCCATGGTTGTTGCAGCTCTGGTTGAGATGACAAGGCTGCAAACTGCGAAAGATTATGGTCTGGTTGATAATCCAAGTGCCACAGTTCCAATGAGTATTTGGTGGTTGGCTCCTCAGTACTTGTTGATGGGATTAGCCGATGTTTTCACAATGGTTGGTTTGCAAGAGTTTTTCTATGACCAGGTGCCAACTGAGTTAAGGAGTGTAGGACTTGCCCTCTACCTCAGTATATTTGGTGTTGGAAGCTTTTTCAGCAGCTTTCTTATCTCGGCTATTGAGAAAGCTACCAGTGGGATGGGTCAAGATAGCTGGTTTTCAAATAATTTGAACCGTGCACATCTTGATTACTTCTATTGGCTACTTGCTGGACTCAGTGTGGTAGAATTGGCTATCTTTGTGTATTTTGCAAAATCTTACATTTATAATAGGAGAGGTACAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

574

Amino Acids

63.26

Weight (kDa)

5.57

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 61 - 512 1e-14 Major Facilitator Superfamily
PTR2 PF00854 109 - 531 2.2e-97 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1704
Acc36I ACCTGC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 1465
AciI CCGC 7 cut(s) 72, 117, 129, 158, 239, 328, 1186
AclI AACGTT 1 cut(s) 255
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 4 cut(s) 3, 27, 231, 303
AcsI RAATTY 2 cut(s) 693, 876
AcuI CTGAAG 3 cut(s) 828, 879, 1121
AcyI GRCGYC 1 cut(s) 272
AfaI GTAC 5 cut(s) 637, 967, 1197, 1400, 1717
AfiI CCNNNNNNNGG 1 cut(s) 676
AgeI ACCGGT 1 cut(s) 753
AgsI TTSAA 7 cut(s) 461, 598, 907, 946, 983, 1599, 1609
AhdI GACNNNNNGTC 1 cut(s) 95
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 2 cut(s) 1108, 1461
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
Alw21I GWGCWC 1 cut(s) 1618
Alw26I GTCTC 1 cut(s) 27
Alw44I GTGCAC 1 cut(s) 1614
AlwI GGATC 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 870
AoxI GGCC 7 cut(s) 3, 27, 69, 212, 231, 303, 387
ApaLI GTGCAC 1 cut(s) 1614
ApeKI GCWGC 3 cut(s) 1295, 1318, 1536
ApoI RAATTY 2 cut(s) 693, 876
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AsiGI ACCGGT 1 cut(s) 753
Asp700I GAANNNNTTC 1 cut(s) 838
AspS9I GGNCC 5 cut(s) 97, 105, 212, 626, 712
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 878, 1063
AvaI CYCGRG 1 cut(s) 870
AvaII GGWCC 4 cut(s) 97, 105, 626, 712
AxyI CCTNAGG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 1618
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1465
Bbv12I GWGCWC 1 cut(s) 1618
BbvI GCAGC 3 cut(s) 1305, 1307, 1548
BccI CCATC 5 cut(s) 341, 548, 703, 1402, 1570
BceAI ACGGC 4 cut(s) 59, 246, 252, 285
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 2 cut(s) 1110, 1463
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 5 cut(s) 491, 744, 1154, 1206, 1218
BfmI CTRYAG 1 cut(s) 1244
BfuAI ACCTGC 1 cut(s) 614
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 5 cut(s) 72, 240, 1296, 1319, 1537
BlsI GCNGC 5 cut(s) 73, 241, 1297, 1320, 1538
BmcAI AGTACT 1 cut(s) 1400
Bme1390I CCNGG 3 cut(s) 95, 1110, 1463
Bme18I GGWCC 4 cut(s) 97, 105, 626, 712
BmeRI GACNNNNNGTC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 870
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 5 cut(s) 97, 105, 212, 626, 712
BmiI GGNNCC 3 cut(s) 628, 1392, 1467
BmrFI CCNGG 3 cut(s) 95, 1110, 1463
BmsI GCATC 1 cut(s) 1242
BpmI CTGGAG 1 cut(s) 918
Bpu10I CCTNAGC 1 cut(s) 671
BpuMI CCSGG 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 272
BsaJI CCNNGG 4 cut(s) 85, 234, 316, 1284
BsaWI WCCGGW 1 cut(s) 753
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse118I RCCGGY 1 cut(s) 753
Bse1I ACTGG 1 cut(s) 1569
Bse21I CCTNAGG 1 cut(s) 101
BseBI CCWGG 2 cut(s) 1110, 1463
BseDI CCNNGG 4 cut(s) 85, 234, 316, 1284
BseGI GGATG 2 cut(s) 1269, 1581
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMII CTCAG 6 cut(s) 115, 662, 1409, 1464, 1519, 1666
BseNI ACTGG 1 cut(s) 1569
BseRI GAGGAG 2 cut(s) 341, 1383
BseSI GKGCMC 1 cut(s) 1618
BseXI GCAGC 3 cut(s) 1305, 1307, 1548
BseYI CCCAGC 1 cut(s) 675
BsgI GTGCAG 1 cut(s) 642
BshFI GGCC 7 cut(s) 5, 29, 71, 214, 233, 305, 389
BshNI GGYRCC 1 cut(s) 1465
BshTI ACCGGT 1 cut(s) 753
BsiHKAI GWGCWC 1 cut(s) 1618
BsiHKCI CYCGRG 1 cut(s) 870
BsiSI CCGG 3 cut(s) 26, 95, 754
BslFI GGGAC 3 cut(s) 275, 378, 612
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 3 cut(s) 275, 378, 612
BsmI GAATGC 2 cut(s) 578, 996
BsnI GGCC 7 cut(s) 5, 29, 71, 214, 233, 305, 389
BsoBI CYCGRG 1 cut(s) 870
Bsp1286I GDGCHC 1 cut(s) 1618
Bsp1407I TGTACA 1 cut(s) 635
Bsp143I GATC 2 cut(s) 544, 562
Bsp19I CCATGG 1 cut(s) 1284
BspACI CCGC 7 cut(s) 72, 117, 129, 158, 239, 328, 1186
BspANI GGCC 7 cut(s) 5, 29, 71, 214, 233, 305, 389
BspCNI CTCAG 6 cut(s) 114, 663, 1408, 1465, 1518, 1665
BspLI GGNNCC 3 cut(s) 628, 1392, 1467
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 1 cut(s) 557
BspT107I GGYRCC 1 cut(s) 1465
BsrFI RCCGGY 1 cut(s) 753
BsrGI TGTACA 1 cut(s) 635
BsrI ACTGG 1 cut(s) 1569
BssAI RCCGGY 1 cut(s) 753
BssECI CCNNGG 4 cut(s) 85, 234, 316, 1284
BssMI GATC 2 cut(s) 544, 562
BssNI GRCGYC 1 cut(s) 272
BssT1I CCWWGG 2 cut(s) 85, 1284
Bst2UI CCWGG 2 cut(s) 1110, 1463
Bst4CI ACNGT 5 cut(s) 419, 970, 1056, 1366, 1613
Bst6I CTCTTC 1 cut(s) 855
BstACI GRCGYC 1 cut(s) 272
BstAUI TGTACA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 930
BstDEI CTNAG 7 cut(s) 101, 404, 671, 1395, 1473, 1505, 1652
BstDSI CCRYGG 2 cut(s) 234, 1284
BstF5I GGATG 2 cut(s) 1269, 1581
BstKTI GATC 2 cut(s) 547, 565
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 2 cut(s) 544, 562
BstMWI GCNNNNNNNGC 5 cut(s) 239, 302, 539, 929, 1533
BstNI CCWGG 2 cut(s) 1110, 1463
BstNSI RCATGY 1 cut(s) 1029
BstSCI CCNGG 3 cut(s) 93, 1108, 1461
BstSFI CTRYAG 1 cut(s) 1244
BstSLI GKGCMC 1 cut(s) 1618
BstV1I GCAGC 3 cut(s) 1305, 1307, 1548
BstX2I RGATCY 1 cut(s) 562
BstXI CCANNNNNNTGG 1 cut(s) 1576
BstYI RGATCY 1 cut(s) 562
Bsu36I CCTNAGG 1 cut(s) 101
BsuRI GGCC 7 cut(s) 5, 29, 71, 214, 233, 305, 389
BtgI CCRYGG 2 cut(s) 234, 1284
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 1269, 1581
BtsIMutI CAGTG 2 cut(s) 1576, 1660
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 1 cut(s) 930
Cfr10I RCCGGY 1 cut(s) 753
Cfr13I GGNCC 5 cut(s) 97, 105, 212, 626, 712
CseI GACGC 3 cut(s) 261, 330, 751
CsiI ACCWGGT 1 cut(s) 1461
Csp6I GTAC 5 cut(s) 636, 966, 1196, 1399, 1716
CspAI ACCGGT 1 cut(s) 753
CviAII CATG 4 cut(s) 444, 1026, 1090, 1285
CviQI GTAC 5 cut(s) 636, 966, 1196, 1399, 1716
DdeI CTNAG 7 cut(s) 101, 404, 671, 1395, 1473, 1505, 1652
DpnI GATC 2 cut(s) 546, 564
DpnII GATC 2 cut(s) 544, 562
DriI GACNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 4 cut(s) 3, 27, 231, 303
Eam1104I CTCTTC 1 cut(s) 855
Eam1105I GACNNNNNGTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 855
EciI GGCGGA 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 85, 1284
Eco47I GGWCC 4 cut(s) 97, 105, 626, 712
Eco57I CTGAAG 3 cut(s) 828, 879, 1121
Eco81I CCTNAGG 1 cut(s) 101
Eco88I CYCGRG 1 cut(s) 870
EcoO109I RGGNCCY 2 cut(s) 212, 626
EcoRI GAATTC 2 cut(s) 693, 876
EcoRII CCWGG 2 cut(s) 1108, 1461
EcoT14I CCWWGG 2 cut(s) 85, 1284
ErhI CCWWGG 2 cut(s) 85, 1284
FaeI CATG 4 cut(s) 447, 1029, 1093, 1288
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 3 cut(s) 275, 378, 612
FatI CATG 4 cut(s) 443, 1025, 1089, 1284
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 5 cut(s) 72, 240, 1296, 1319, 1537
FokI GGATG 2 cut(s) 1276, 1588
Fsp4HI GCNGC 5 cut(s) 72, 240, 1296, 1319, 1537
FspBI CTAG 5 cut(s) 491, 744, 1154, 1206, 1218
GluI GCNGC 5 cut(s) 72, 240, 1296, 1319, 1537
GsaI CCCAGC 1 cut(s) 679
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 7 cut(s) 5, 29, 71, 214, 233, 305, 389
HapII CCGG 3 cut(s) 26, 95, 754
HgaI GACGC 3 cut(s) 261, 330, 751
Hin1I GRCGYC 1 cut(s) 272
Hin1II CATG 4 cut(s) 447, 1029, 1093, 1288
HincII GTYRAC 3 cut(s) 79, 228, 378
HindII GTYRAC 3 cut(s) 79, 228, 378
HindIII AAGCTT 1 cut(s) 1525
HinfI GANTC 4 cut(s) 20, 308, 866, 1650
HpaII CCGG 3 cut(s) 26, 95, 754
HphI GGTGA 2 cut(s) 878, 1063
Hpy166II GTNNAC 9 cut(s) 79, 108, 228, 378, 438, 512, 636, 966, 1616
Hpy188I TCNGA 7 cut(s) 207, 320, 415, 767, 859, 898, 1140
Hpy188III TCNNGA 6 cut(s) 461, 566, 870, 935, 1583, 1622
Hpy8I GTNNAC 9 cut(s) 79, 108, 228, 378, 438, 512, 636, 966, 1616
Hpy99I CGWCG 6 cut(s) 56, 59, 62, 277, 974, 977
HpyAV CCTTC 5 cut(s) 743, 940, 952, 1075, 1145
HpyCH4III ACNGT 5 cut(s) 419, 970, 1056, 1366, 1613
HpyCH4IV ACGT 2 cut(s) 60, 255
HpyCH4V TGCA 8 cut(s) 576, 623, 1052, 1295, 1321, 1444, 1616, 1688
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 302, 539, 929, 1533
HpyF3I CTNAG 7 cut(s) 101, 404, 671, 1395, 1473, 1505, 1652
HpySE526I ACGT 2 cut(s) 60, 255
Hsp92I GRCGYC 1 cut(s) 272
Hsp92II CATG 4 cut(s) 447, 1029, 1093, 1288
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 2 cut(s) 544, 562
LmnI GCTCC 4 cut(s) 293, 539, 937, 1396
Lsp1109I GCAGC 3 cut(s) 1305, 1307, 1548
LweI GCATC 1 cut(s) 1242
MabI ACCWGGT 1 cut(s) 1461
MaeI CTAG 5 cut(s) 491, 744, 1154, 1206, 1218
MaeII ACGT 2 cut(s) 60, 255
MalI GATC 2 cut(s) 546, 564
MboI GATC 2 cut(s) 544, 562
MfeI CAATTG 1 cut(s) 598
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 1 cut(s) 1618
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 29, 1644
MmeI TCCRAC 2 cut(s) 213, 1501
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 838
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 821, 1478
MslI CAYNNNNRTG 1 cut(s) 1238
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 26, 95, 754
MspR9I CCNGG 3 cut(s) 95, 1110, 1463
MunI CAATTG 1 cut(s) 598
Mva1269I GAATGC 2 cut(s) 578, 996
MvaI CCWGG 2 cut(s) 1110, 1463
MwoI GCNNNNNNNGC 5 cut(s) 239, 302, 539, 929, 1533
NciI CCSGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 1284
NdeI CATATG 1 cut(s) 1036
NdeII GATC 2 cut(s) 544, 562
NlaIII CATG 4 cut(s) 447, 1029, 1093, 1288
NlaIV GGNNCC 3 cut(s) 628, 1392, 1467
NmeAIII GCCGAG 3 cut(s) 365, 433, 1529
NspI RCATGY 1 cut(s) 1029
PaeR7I CTCGAG 1 cut(s) 870
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 2 cut(s) 578, 996
PdmI GAANNNNTTC 1 cut(s) 838
PfeI GAWTC 2 cut(s) 308, 866
PinAI ACCGGT 1 cut(s) 753
PkrI GCNGC 5 cut(s) 73, 241, 1297, 1320, 1538
PleI GAGTC 2 cut(s) 28, 1644
PpsI GAGTC 2 cut(s) 28, 1644
PpuMI RGGWCCY 1 cut(s) 626
PsiI TTATAA 1 cut(s) 1704
Psp1406I AACGTT 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 626
Psp6I CCWGG 2 cut(s) 1108, 1461
PspFI CCCAGC 1 cut(s) 675
PspGI CCWGG 2 cut(s) 1108, 1461
PspN4I GGNNCC 3 cut(s) 628, 1392, 1467
PspPI GGNCC 5 cut(s) 97, 105, 212, 626, 712
PspPPI RGGWCCY 1 cut(s) 626
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 5 cut(s) 637, 967, 1197, 1400, 1717
RsaNI GTAC 5 cut(s) 636, 966, 1196, 1399, 1716
RseI CAYNNNNRTG 1 cut(s) 1238
SaqAI TTAA 2 cut(s) 821, 1478
SatI GCNGC 5 cut(s) 72, 240, 1296, 1319, 1537
Sau3AI GATC 2 cut(s) 544, 562
Sau96I GGNCC 5 cut(s) 97, 105, 212, 626, 712
ScaI AGTACT 1 cut(s) 1400
SchI GAGTC 2 cut(s) 29, 1644
ScrFI CCNGG 3 cut(s) 95, 1110, 1463
SduI GDGCHC 1 cut(s) 1618
SexAI ACCWGGT 1 cut(s) 1461
SfaNI GCATC 1 cut(s) 1242
SfcI CTRYAG 1 cut(s) 1244
Sfr274I CTCGAG 1 cut(s) 870
SinI GGWCC 4 cut(s) 97, 105, 626, 712
SlaI CTCGAG 1 cut(s) 870
SmiMI CAYNNNNRTG 1 cut(s) 1238
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
SsiI CCGC 7 cut(s) 72, 117, 129, 158, 239, 328, 1186
SspMI CTAG 5 cut(s) 491, 744, 1154, 1206, 1218
StyD4I CCNGG 3 cut(s) 93, 1108, 1461
StyI CCWWGG 2 cut(s) 85, 1284
TaaI ACNGT 5 cut(s) 419, 970, 1056, 1366, 1613
TaiI ACGT 2 cut(s) 63, 258
TaqI TCGA 4 cut(s) 51, 871, 975, 1061
TatI WGTACW 2 cut(s) 635, 1398
TauI GCSGC 2 cut(s) 74, 242
TfiI GAWTC 2 cut(s) 308, 866
Tru1I TTAA 2 cut(s) 821, 1478
Tru9I TTAA 2 cut(s) 821, 1478
TscAI CASTG 2 cut(s) 1576, 1660
TseI GCWGC 3 cut(s) 1295, 1318, 1536
TspDTI ATGAA 3 cut(s) 127, 1106, 1159
TspGWI ACGGA 2 cut(s) 279, 774
TspRI CASTG 2 cut(s) 1576, 1660
VneI GTGCAC 1 cut(s) 1614
VpaK11BI GGWCC 4 cut(s) 97, 105, 626, 712
XapI RAATTY 2 cut(s) 693, 876
XceI RCATGY 1 cut(s) 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 1377
XhoI CTCGAG 1 cut(s) 870
XmnI GAANNNNTTC 1 cut(s) 838
XspI CTAG 5 cut(s) 491, 744, 1154, 1206, 1218
ZrmI AGTACT 1 cut(s) 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.