Rh4DG258800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003111
Physical Location & Seq
Forward (+)
47509993 .. 47512545
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG258800.1

Sequence Viewer

Length: 1215 bp
ATGATGATGGAATTGGCCGGCGGTTTTAAGGCCAAGCACGGTCATTTCAGGGCCGGACTGGATTCCGGCGAGATTTTGGTGATCAAGCTGCCTGATATGAAGGTACTGCGTATTATTTGCCGGTCAGTTTTTCTGGCTTTGGTTATGCTCACACTGCCCTGCATTGGGTCTATTTTTAAGCGTGTAACTGTTTCTGAATTGGAAAGTAGTGGGTCTGAAATTTTCAAATTTGAGCAATTGGGTATGCTTTTCAATGATTTGGCTGGTGAAGGCCTTCGCAGGGAGGGTGACAAGTCTCTCATTGTGAGTCCTGGCAATGTTGGTATGATTCATGCTATTCGTGGTTTGAATGTTGATGATAATGGATTTGAAATTGCTATGGATTCTGATTTGGAGAGAAAGAGCTCGGTCAAGGACGAGTCTGTGGATTTTGTTTTCGCATTTGATTTGGGTGATGCTAAATTTGTGGATCGCGTCTTGAAAGTTGGTGGCATTGTTGCTGTTCCTCTAAGCAATGACCCTTCAGATGCTTTTAGCAAAAAATCCAATTACAAGATTGTGTATCTTCGCCGATACGCTTCAATTTTTGTGGCGATGAGGAAGACCGGTCCGGCTGGTGAGCTGCAGGAGAATCCTGGCCGCCGGCGGCTTTGCCAATTTGGAAGTGGGGCCAAGGAGACCGTCCTGCAGGGTCTTGAAGATGTGTTGCTTGAGCCACCAAGACGTGCTTTGGCAAAATCAAGCCAGTACTTGAAGAAGATGAAGTTCCTCCCTGACCTGTTGGGGAATTCTCTTGAAAATTACGAGCGCCGTGTGTTCGTTAATGTGGACTTGAATGAGAAAAAAGGTGGCGCAATTGAATGGTTTCATCAAAACTATCCGAAAATGAATACGGATTTTGAAGTACACAACCTTGAAATAGAAGAACAACAAGATGAAGAAGCTTCAAGCAAGCCGCAGACTGATGTTTCGGGTTGGCTAAAGGAGAATGTGAAGGAAGAGGACTATGTTGTGATGAAAGCAGAAGCAGAAGTTGTTGTGGATATGATGCAGAAGAGCACAATCTGTCTGGTGGATGAGTTGTTTTTGGAGTGTAACAATGAATGGTGGCAGAATGGAAAGAGTGACAAGAGCAAGAGAGCATATTGGGAGTGCATTGCTCTACATGGAAGGCTAAGGGATCTAGGAGTTGCTGTGCACCAGTGGTTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

45.62

Weight (kDa)

5.59

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 229 - 403 1.5e-79 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 474
AciI CCGC 4 cut(s) 21, 640, 646, 956
AclWI GGATC 2 cut(s) 477, 1188
AcoI YGGCCR 2 cut(s) 15, 637
AcsI RAATTY 4 cut(s) 219, 227, 461, 787
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 3 cut(s) 105, 749, 906
AfiI CCNNNNNNNGG 3 cut(s) 164, 165, 280
AgeI ACCGGT 1 cut(s) 605
AjiI CACGTC 1 cut(s) 725
AjnI CCWGG 2 cut(s) 310, 634
AluBI AGCT 4 cut(s) 88, 405, 622, 944
AluI AGCT 4 cut(s) 88, 405, 622, 944
Alw21I GWGCWC 3 cut(s) 407, 1061, 1200
Alw26I GTCTC 2 cut(s) 300, 671
Alw44I GTGCAC 1 cut(s) 1196
AlwI GGATC 2 cut(s) 477, 1188
AoxI GGCC 6 cut(s) 15, 30, 51, 271, 637, 669
ApaLI GTGCAC 1 cut(s) 1196
ApeKI GCWGC 2 cut(s) 88, 622
ApoI RAATTY 4 cut(s) 219, 227, 461, 787
ArsI GACNNNNNNTTYG 2 cut(s) 952, 984
AsiGI ACCGGT 1 cut(s) 605
Asp700I GAANNNNTTC 2 cut(s) 273, 864
AspLEI GCGC 2 cut(s) 810, 854
AspS9I GGNCC 3 cut(s) 51, 608, 669
AsuHPI GGTGA 5 cut(s) 91, 278, 299, 464, 629
AvaII GGWCC 1 cut(s) 608
BaeGI GKGCMC 1 cut(s) 1200
BanII GRGCYC 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 608
Bbv12I GWGCWC 3 cut(s) 407, 1061, 1200
BbvI GCAGC 2 cut(s) 75, 609
BceAI ACGGC 1 cut(s) 795
BciT130I CCWGG 2 cut(s) 312, 636
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 300, 671
BfaI CTAG 1 cut(s) 1184
BfmI CTRYAG 2 cut(s) 623, 686
BfoI RGCGCY 1 cut(s) 811
BisI GCNGC 5 cut(s) 89, 623, 640, 647, 956
BlsI GCNGC 5 cut(s) 90, 624, 641, 648, 957
BmcAI AGTACT 1 cut(s) 749
Bme1390I CCNGG 2 cut(s) 312, 636
Bme18I GGWCC 1 cut(s) 608
BmgBI CACGTC 1 cut(s) 725
BmgT120I GGNCC 3 cut(s) 51, 608, 669
BmiI GGNNCC 1 cut(s) 670
BmrFI CCNGG 2 cut(s) 312, 636
BmsI GCATC 3 cut(s) 445, 517, 1038
BpiI GAAGAC 1 cut(s) 608
Bpu10I CCTNAGC 1 cut(s) 1175
BpuEI CTTGAG 1 cut(s) 731
BsaI GGTCTC 1 cut(s) 671
BsaJI CCNNGG 1 cut(s) 672
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 2 cut(s) 1179, 1209
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 165, 280
Bse118I RCCGGY 4 cut(s) 17, 120, 605, 642
Bse1I ACTGG 3 cut(s) 63, 745, 1201
Bse3DI GCAATG 3 cut(s) 322, 520, 1155
BseBI CCWGG 2 cut(s) 312, 636
BseDI CCNNGG 1 cut(s) 672
BseGI GGATG 1 cut(s) 1081
BseLI CCNNNNNNNGG 3 cut(s) 164, 165, 280
BseMI GCAATG 3 cut(s) 322, 520, 1155
BseNI ACTGG 3 cut(s) 63, 745, 1201
BseSI GKGCMC 1 cut(s) 1200
BseXI GCAGC 2 cut(s) 75, 609
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 6 cut(s) 17, 32, 53, 273, 639, 671
BshTI ACCGGT 1 cut(s) 605
BsiHKAI GWGCWC 3 cut(s) 407, 1061, 1200
BsiSI CCGG 7 cut(s) 18, 54, 66, 121, 606, 611, 643
BslI CCNNNNNNNGG 3 cut(s) 164, 165, 280
BsmAI GTCTC 2 cut(s) 300, 671
BsnI GGCC 6 cut(s) 17, 32, 53, 273, 639, 671
Bso31I GGTCTC 1 cut(s) 671
Bsp1286I GDGCHC 3 cut(s) 407, 1061, 1200
Bsp143I GATC 3 cut(s) 81, 469, 1180
BspACI CCGC 4 cut(s) 21, 640, 646, 956
BspANI GGCC 6 cut(s) 17, 32, 53, 273, 639, 671
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 1 cut(s) 670
BspMAI CTGCAG 2 cut(s) 627, 690
BspPI GGATC 2 cut(s) 477, 1188
BspQI GCTCTTC 1 cut(s) 1049
BspTNI GGTCTC 1 cut(s) 671
BsrDI GCAATG 3 cut(s) 322, 520, 1155
BsrFI RCCGGY 4 cut(s) 17, 120, 605, 642
BsrI ACTGG 3 cut(s) 63, 745, 1201
BssAI RCCGGY 4 cut(s) 17, 120, 605, 642
BssECI CCNNGG 1 cut(s) 672
BssMI GATC 3 cut(s) 81, 469, 1180
BssT1I CCWWGG 1 cut(s) 672
Bst2UI CCWGG 2 cut(s) 312, 636
Bst4CI ACNGT 3 cut(s) 41, 190, 682
Bst6I CTCTTC 2 cut(s) 993, 1049
BstC8I GCNNGC 3 cut(s) 19, 644, 953
BstDEI CTNAG 2 cut(s) 509, 1175
BstF5I GGATG 1 cut(s) 1081
BstFNI CGCG 1 cut(s) 474
BstH2I RGCGCY 1 cut(s) 811
BstHHI GCGC 2 cut(s) 810, 854
BstKTI GATC 3 cut(s) 84, 472, 1183
BstMAI GTCTC 2 cut(s) 300, 671
BstMBI GATC 3 cut(s) 81, 469, 1180
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNI CCWGG 2 cut(s) 312, 636
BstSCI CCNGG 2 cut(s) 310, 634
BstSFI CTRYAG 2 cut(s) 623, 686
BstSLI GKGCMC 1 cut(s) 1200
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 2 cut(s) 75, 609
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 1180
BstYI RGATCY 1 cut(s) 1180
BsuRI GGCC 6 cut(s) 17, 32, 53, 273, 639, 671
BtgZI GCGATG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 725
BtsCI GGATG 1 cut(s) 1081
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 2 cut(s) 152, 1208
Cac8I GCNNGC 3 cut(s) 19, 644, 953
CfoI GCGC 2 cut(s) 810, 854
Cfr10I RCCGGY 4 cut(s) 17, 120, 605, 642
Cfr13I GGNCC 3 cut(s) 51, 608, 669
CpoI CGGWCCG 1 cut(s) 608
CseI GACGC 1 cut(s) 463
Csp6I GTAC 3 cut(s) 104, 748, 905
CspAI ACCGGT 1 cut(s) 605
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CspI CGGWCCG 1 cut(s) 608
CviAII CATG 2 cut(s) 332, 1166
CviQI GTAC 3 cut(s) 104, 748, 905
DdeI CTNAG 2 cut(s) 509, 1175
DpnI GATC 3 cut(s) 83, 471, 1182
DpnII GATC 3 cut(s) 81, 469, 1180
EaeI YGGCCR 2 cut(s) 15, 637
Eam1104I CTCTTC 2 cut(s) 993, 1049
EarI CTCTTC 2 cut(s) 993, 1049
Ecl136II GAGCTC 1 cut(s) 405
Eco130I CCWWGG 1 cut(s) 672
Eco147I AGGCCT 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 407
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 405
Eco57I CTGAAG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 310, 634
EcoT14I CCWWGG 1 cut(s) 672
EcoT38I GRGCYC 1 cut(s) 407
ErhI CCWWGG 1 cut(s) 672
FaeI CATG 2 cut(s) 335, 1169
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FatI CATG 2 cut(s) 331, 1165
FbaI TGATCA 1 cut(s) 81
Fnu4HI GCNGC 5 cut(s) 89, 623, 640, 647, 956
FokI GGATG 1 cut(s) 1088
FriOI GRGCYC 1 cut(s) 407
Fsp4HI GCNGC 5 cut(s) 89, 623, 640, 647, 956
FspBI CTAG 1 cut(s) 1184
GlaI GCGC 2 cut(s) 809, 853
GluI GCNGC 5 cut(s) 89, 623, 640, 647, 956
HaeII RGCGCY 1 cut(s) 811
HaeIII GGCC 6 cut(s) 17, 32, 53, 273, 639, 671
HapII CCGG 7 cut(s) 18, 54, 66, 121, 606, 611, 643
HgaI GACGC 1 cut(s) 463
HhaI GCGC 2 cut(s) 810, 854
Hin1II CATG 2 cut(s) 335, 1169
Hin6I GCGC 2 cut(s) 808, 852
HinP1I GCGC 2 cut(s) 808, 852
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 6 cut(s) 62, 307, 328, 383, 419, 631
HpaII CCGG 7 cut(s) 18, 54, 66, 121, 606, 611, 643
HphI GGTGA 5 cut(s) 91, 278, 299, 464, 629
Hpy166II GTNNAC 3 cut(s) 829, 907, 1198
Hpy188I TCNGA 5 cut(s) 196, 217, 388, 526, 882
Hpy188III TCNNGA 3 cut(s) 478, 695, 794
Hpy8I GTNNAC 3 cut(s) 829, 907, 1198
HpyAV CCTTC 6 cut(s) 94, 263, 284, 531, 988, 1164
HpyCH4III ACNGT 3 cut(s) 41, 190, 682
HpyCH4IV ACGT 1 cut(s) 724
HpyCH4V TGCA 6 cut(s) 162, 625, 688, 1051, 1155, 1198
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 2 cut(s) 509, 1175
HpySE526I ACGT 1 cut(s) 724
Hsp92II CATG 2 cut(s) 335, 1169
HspAI GCGC 2 cut(s) 808, 852
KroI GCCGGC 2 cut(s) 17, 642
KroNI GCCGGC 2 cut(s) 19, 644
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 3 cut(s) 81, 469, 1180
LguI GCTCTTC 1 cut(s) 1049
Lsp1109I GCAGC 2 cut(s) 75, 609
LweI GCATC 3 cut(s) 445, 517, 1038
MaeI CTAG 1 cut(s) 1184
MaeII ACGT 1 cut(s) 724
MaeIII GTNAC 4 cut(s) 184, 287, 1094, 1124
MalI GATC 3 cut(s) 83, 471, 1182
MboI GATC 3 cut(s) 81, 469, 1180
MboII GAAGA 9 cut(s) 557, 613, 710, 766, 769, 935, 950, 1010, 1066
MfeI CAATTG 2 cut(s) 236, 855
MflI RGATCY 1 cut(s) 1180
MhlI GDGCHC 3 cut(s) 407, 1061, 1200
MlyI GAGTC 2 cut(s) 316, 428
MnlI CCTC 5 cut(s) 277, 516, 591, 779, 994
MreI CGCCGGCG 1 cut(s) 642
MroNI GCCGGC 2 cut(s) 17, 642
MroXI GAANNNNTTC 2 cut(s) 273, 864
MseI TTAA 3 cut(s) 27, 177, 822
MspI CCGG 7 cut(s) 18, 54, 66, 121, 606, 611, 643
MspR9I CCNGG 2 cut(s) 312, 636
MunI CAATTG 2 cut(s) 236, 855
MvaI CCWGG 2 cut(s) 312, 636
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 154
NaeI GCCGGC 2 cut(s) 19, 644
NdeII GATC 3 cut(s) 81, 469, 1180
NgoMIV GCCGGC 2 cut(s) 17, 642
NlaIII CATG 2 cut(s) 335, 1169
NlaIV GGNNCC 1 cut(s) 670
NmuCI GTSAC 2 cut(s) 287, 1124
PceI AGGCCT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 1049
PdiI GCCGGC 2 cut(s) 19, 644
PdmI GAANNNNTTC 2 cut(s) 273, 864
PfeI GAWTC 4 cut(s) 62, 328, 383, 631
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 5 cut(s) 90, 624, 641, 648, 957
PleI GAGTC 2 cut(s) 315, 427
PpsI GAGTC 2 cut(s) 315, 427
Psp124BI GAGCTC 1 cut(s) 407
Psp6I CCWGG 2 cut(s) 310, 634
PspGI CCWGG 2 cut(s) 310, 634
PspN4I GGNNCC 1 cut(s) 670
PspPI GGNCC 3 cut(s) 51, 608, 669
PstI CTGCAG 2 cut(s) 627, 690
PsuI RGATCY 1 cut(s) 1180
RsaI GTAC 3 cut(s) 105, 749, 906
RsaNI GTAC 3 cut(s) 104, 748, 905
Rsr2I CGGWCCG 1 cut(s) 608
RsrII CGGWCCG 1 cut(s) 608
SacI GAGCTC 1 cut(s) 407
SapI GCTCTTC 1 cut(s) 1049
SaqAI TTAA 3 cut(s) 27, 177, 822
SatI GCNGC 5 cut(s) 89, 623, 640, 647, 956
Sau3AI GATC 3 cut(s) 81, 469, 1180
Sau96I GGNCC 3 cut(s) 51, 608, 669
SbfI CCTGCAGG 1 cut(s) 690
ScaI AGTACT 1 cut(s) 749
SchI GAGTC 2 cut(s) 316, 428
ScrFI CCNGG 2 cut(s) 312, 636
SdaI CCTGCAGG 1 cut(s) 690
SduI GDGCHC 3 cut(s) 407, 1061, 1200
SetI ASST 9 cut(s) 90, 105, 407, 624, 727, 780, 850, 915, 946
SfaNI GCATC 3 cut(s) 445, 517, 1038
SfcI CTRYAG 2 cut(s) 623, 686
SgrAI CRCCGGYG 1 cut(s) 642
SinI GGWCC 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
Sse8387I CCTGCAGG 1 cut(s) 690
SseBI AGGCCT 1 cut(s) 273
SsiI CCGC 4 cut(s) 21, 640, 646, 956
SspMI CTAG 1 cut(s) 1184
SstI GAGCTC 1 cut(s) 407
StuI AGGCCT 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 310, 634
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 3 cut(s) 41, 190, 682
TaiI ACGT 1 cut(s) 727
TaqII GACCGA 1 cut(s) 397
TatI WGTACW 2 cut(s) 747, 904
TauI GCSGC 3 cut(s) 642, 649, 958
TfiI GAWTC 4 cut(s) 62, 328, 383, 631
Tru1I TTAA 3 cut(s) 27, 177, 822
Tru9I TTAA 3 cut(s) 27, 177, 822
TscAI CASTG 2 cut(s) 159, 1208
TseFI GTSAC 2 cut(s) 287, 1124
TseI GCWGC 2 cut(s) 88, 622
Tsp45I GTSAC 2 cut(s) 287, 1124
TspDTI ATGAA 8 cut(s) 113, 320, 776, 857, 902, 951, 1031, 1116
TspGWI ACGGA 1 cut(s) 908
TspRI CASTG 2 cut(s) 159, 1208
VneI GTGCAC 1 cut(s) 1196
VpaK11BI GGWCC 1 cut(s) 608
XapI RAATTY 4 cut(s) 219, 227, 461, 787
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XmnI GAANNNNTTC 2 cut(s) 273, 864
XspI CTAG 1 cut(s) 1184
ZrmI AGTACT 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.