pycom13g13200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003111
Physical Location & Seq
Reverse (-)
9341735 .. 9342969
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g13200.2

Sequence Viewer

Length: 1185 bp
ATGGAATTGGCTGACGGGTTTGCAGCCAAGGATCAAAACAAGGTGAAGCACCTTCATGGTGGACAGATGGGATTGGATTCCGGGACTTACTTGGTGATCAATCTTCCCGATTCCAAGGCATTGCGGATTATTCCGTGCGCCGTTTTTCTTGGTTTAGTTATTCTTACACTGCCCTGCATTGGAACAATTCTGAGGGGATTAAAAGTTTCCCGATTGGATTTGAATTTCGAATCCGAAAATTTCAATTTCAAGCAGTTGGATCTGCTTTTTCGTGATTTGGCCGGCGAAGGCCTCCGCAGAAAGGGTGATACATCTCTCATTGTGGCTCCTGGCAATGCCGGTACGCTTCAAAATCTTCACCCTTTTGATTTCAATGGATTTGATTTCGTTGTGGATTCGGAGTCGGTGCCCAAAAGCTCGTTCTTGGACGAGTCTATGGATTTCGTTTTCGCATTCAACTTGGCCGATGCTAAATTTGCTGATCGCATTCTGAAGATTGGCGGCATTGTTGTCGTTCCAATAAGCAACGACCCTTCGAACGCCTTTAGGCCAAAACCCAATTACAAAATCGTGTATCTCCGCCGATACGCTGCCACTTTTGTGGCAATGAGGAAGATAAGTCCGGCTTATGACTTGCCCGTGACGTCCCGGCGGCTTTGTCAGTTGGAAACCGAGGAAAAGAAGACGGTGTTAGAGGGTCTGGAAGACGTGCTGCTGGAACCGCCGAGGCTCATTTTGGCCAAATCGAATGAATACTTGAAGAAAATCAAGTTCCTCCCCGAGTTGTTGGGTGTTTCCCTCGACAGTTACAACCGCCGAGTGTTTGTCAATGTAGAGCTAAACGAGGAAAACCGCGGCATGATCGAATGGTTTGATCAAAACTACCCGAAAATGAACAATGAATTCGAGGTCTACAACCTCGAGGTAGAGCCCGAAGAGGCTTCAAGCACCCCTGAAAACGATGTTTCGGACTGGTTGAAAGACAATGTGAGGGAAGAGGACTACGTTGTGATGAAAGCGGAAGCGGAAGTGGTGGTGGACATGCAACGGATGGTGGCAGAGTGGGAGGAGGAATGGTGGGAGCAAGAGAGCTTATTGGGAATGCATTGCTTTGTATGGGAGGCTGAGGGATCTTGGAGTTGCTGTGCACCAGTGGTTCATGTGATTAAATTGAAGAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

395

Amino Acids

44.86

Weight (kDa)

4.88

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 647
AbsI CCTCGAGG 1 cut(s) 920
AccB1I GGYRCC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 912
AccII CGCG 1 cut(s) 855
AclWI GGATC 3 cut(s) 39, 267, 1138
AcoI YGGCCR 3 cut(s) 279, 462, 738
AcsI RAATTY 5 cut(s) 223, 238, 473, 902, 1179
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 2 cut(s) 179, 301
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 3 cut(s) 417, 838, 1092
AluI AGCT 3 cut(s) 417, 838, 1092
Alw21I GWGCWC 1 cut(s) 1150
Alw44I GTGCAC 1 cut(s) 1146
AlwI GGATC 3 cut(s) 39, 267, 1138
Ama87I CYCGRG 2 cut(s) 779, 920
AoxI GGCC 5 cut(s) 279, 289, 462, 548, 738
ApaLI GTGCAC 1 cut(s) 1146
ApeKI GCWGC 3 cut(s) 23, 590, 712
ApoI RAATTY 5 cut(s) 223, 238, 473, 902, 1179
AspLEI GCGC 1 cut(s) 140
AsuC2I CCSGG 2 cut(s) 82, 649
AsuHPI GGTGA 4 cut(s) 55, 106, 317, 350
AsuII TTCGAA 2 cut(s) 228, 536
AvaI CYCGRG 2 cut(s) 779, 920
BaeGI GKGCMC 2 cut(s) 411, 1150
BalI TGGCCA 1 cut(s) 740
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 933
BbsI GAAGAC 2 cut(s) 689, 711
Bbv12I GWGCWC 1 cut(s) 1150
BbvCI CCTCAGC 1 cut(s) 1125
BbvI GCAGC 3 cut(s) 35, 577, 699
BccI CCATC 2 cut(s) 61, 1045
BceAI ACGGC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 1 cut(s) 330
BclI TGATCA 2 cut(s) 96, 874
BcnI CCSGG 2 cut(s) 82, 649
BisI GCNGC 6 cut(s) 24, 502, 591, 653, 713, 856
BlsI GCNGC 6 cut(s) 25, 503, 592, 654, 714, 857
Bme1390I CCNGG 3 cut(s) 82, 330, 649
BmeT110I CYCGRG 2 cut(s) 779, 920
BmgBI CACGTC 1 cut(s) 709
BmiI GGNNCC 3 cut(s) 327, 408, 720
BmrFI CCNGG 3 cut(s) 82, 330, 649
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 2 cut(s) 689, 711
Bpu10I CCTNAGC 1 cut(s) 1125
Bpu14I TTCGAA 2 cut(s) 228, 536
BpuMI CCSGG 2 cut(s) 82, 649
BsaHI GRCGYC 1 cut(s) 644
BsaJI CCNNGG 5 cut(s) 27, 114, 672, 725, 853
BsaXI ACNNNNNCTCC 4 cut(s) 1061, 1091, 1129, 1159
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 301
Bse118I RCCGGY 2 cut(s) 281, 338
Bse1I ACTGG 2 cut(s) 977, 1151
Bse3DI GCAATG 4 cut(s) 119, 340, 612, 1105
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 27, 114, 672, 725, 853
BseGI GGATG 1 cut(s) 1056
BseLI CCNNNNNNNGG 2 cut(s) 179, 301
BseMI GCAATG 4 cut(s) 119, 340, 612, 1105
BseMII CTCAG 2 cut(s) 182, 1116
BseNI ACTGG 2 cut(s) 977, 1151
BseRI GAGGAG 1 cut(s) 1082
BseSI GKGCMC 2 cut(s) 411, 1150
BseXI GCAGC 3 cut(s) 35, 577, 699
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 5 cut(s) 281, 291, 464, 550, 740
BshNI GGYRCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 1150
BsiHKCI CYCGRG 2 cut(s) 779, 920
BsiSI CCGG 5 cut(s) 81, 282, 339, 623, 649
BslFI GGGAC 2 cut(s) 97, 631
BslI CCNNNNNNNGG 2 cut(s) 179, 301
BsmFI GGGAC 2 cut(s) 97, 631
BsmI GAATGC 3 cut(s) 452, 486, 1107
BsnI GGCC 5 cut(s) 281, 291, 464, 550, 740
BsoBI CYCGRG 2 cut(s) 779, 920
Bsp119I TTCGAA 2 cut(s) 228, 536
Bsp1286I GDGCHC 3 cut(s) 411, 933, 1150
Bsp143I GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
BspANI GGCC 5 cut(s) 281, 291, 464, 550, 740
BspCNI CTCAG 2 cut(s) 183, 1117
BspFNI CGCG 1 cut(s) 855
BspLI GGNNCC 3 cut(s) 327, 408, 720
BspPI GGATC 3 cut(s) 39, 267, 1138
BspT104I TTCGAA 2 cut(s) 228, 536
BspT107I GGYRCC 1 cut(s) 406
BsrDI GCAATG 4 cut(s) 119, 340, 612, 1105
BsrFI RCCGGY 2 cut(s) 281, 338
BsrI ACTGG 2 cut(s) 977, 1151
BssAI RCCGGY 2 cut(s) 281, 338
BssECI CCNNGG 5 cut(s) 27, 114, 672, 725, 853
BssMI GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
BssNI GRCGYC 1 cut(s) 644
BssT1I CCWWGG 2 cut(s) 27, 114
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 688, 806
Bst6I CTCTTC 2 cut(s) 930, 990
BstACI GRCGYC 1 cut(s) 644
BstBI TTCGAA 2 cut(s) 228, 536
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 2 cut(s) 191, 1125
BstDSI CCRYGG 1 cut(s) 853
BstF5I GGATG 1 cut(s) 1056
BstFNI CGCG 1 cut(s) 855
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 7 cut(s) 34, 99, 262, 484, 864, 877, 1133
BstMBI GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
BstMWI GCNNNNNNNGC 2 cut(s) 476, 721
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 3 cut(s) 80, 328, 647
BstSLI GKGCMC 2 cut(s) 411, 1150
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 3 cut(s) 35, 577, 699
BstV2I GAAGAC 2 cut(s) 689, 711
BstX2I RGATCY 2 cut(s) 259, 1130
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 2 cut(s) 259, 1130
BsuRI GGCC 5 cut(s) 281, 291, 464, 550, 740
BtgI CCRYGG 1 cut(s) 853
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 1 cut(s) 1056
BtsI GCAGTG 1 cut(s) 167
BtsIMutI CAGTG 2 cut(s) 167, 1158
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 2 cut(s) 281, 338
Cfr42I CCGCGG 1 cut(s) 856
Csp6I GTAC 1 cut(s) 342
CviAII CATG 4 cut(s) 56, 859, 1042, 1160
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 2 cut(s) 191, 1125
DpnI GATC 7 cut(s) 33, 98, 261, 483, 863, 876, 1132
DpnII GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
EaeI YGGCCR 3 cut(s) 279, 462, 738
Eam1104I CTCTTC 2 cut(s) 930, 990
EarI CTCTTC 2 cut(s) 930, 990
EciI GGCGGA 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 27, 114
Eco147I AGGCCT 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 933
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 2 cut(s) 779, 920
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 27, 114
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 1 cut(s) 933
ErhI CCWWGG 2 cut(s) 27, 114
FaeI CATG 4 cut(s) 59, 862, 1045, 1163
FaiI YATR 7 cut(s) 57, 437, 630, 860, 1043, 1117, 1161
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 2 cut(s) 97, 631
FatI CATG 4 cut(s) 55, 858, 1041, 1159
FbaI TGATCA 2 cut(s) 96, 874
FblI GTMKAC 1 cut(s) 912
Fnu4HI GCNGC 6 cut(s) 24, 502, 591, 653, 713, 856
FokI GGATG 1 cut(s) 1063
FriOI GRGCYC 1 cut(s) 933
Fsp4HI GCNGC 6 cut(s) 24, 502, 591, 653, 713, 856
GlaI GCGC 1 cut(s) 139
GluI GCNGC 6 cut(s) 24, 502, 591, 653, 713, 856
HaeIII GGCC 5 cut(s) 281, 291, 464, 550, 740
HapII CCGG 5 cut(s) 81, 282, 339, 623, 649
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 644
Hin1II CATG 4 cut(s) 59, 862, 1045, 1163
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 6 cut(s) 77, 110, 230, 395, 401, 431
HpaII CCGG 5 cut(s) 81, 282, 339, 623, 649
HphI GGTGA 4 cut(s) 55, 106, 317, 350
Hpy166II GTNNAC 4 cut(s) 62, 913, 1039, 1148
Hpy188I TCNGA 5 cut(s) 192, 235, 400, 492, 970
Hpy188III TCNNGA 4 cut(s) 107, 210, 272, 701
Hpy8I GTNNAC 4 cut(s) 62, 913, 1039, 1148
HpyAV CCTTC 3 cut(s) 62, 281, 543
HpyCH4III ACNGT 2 cut(s) 688, 806
HpyCH4IV ACGT 3 cut(s) 644, 708, 1005
HpyCH4V TGCA 5 cut(s) 23, 177, 1045, 1105, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 721
HpyF3I CTNAG 2 cut(s) 191, 1125
HpySE526I ACGT 3 cut(s) 644, 708, 1005
Hsp92I GRCGYC 1 cut(s) 644
Hsp92II CATG 4 cut(s) 59, 862, 1045, 1163
HspAI GCGC 1 cut(s) 138
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 96, 874
KspI CCGCGG 1 cut(s) 856
Kzo9I GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
LmnI GCTCC 2 cut(s) 331, 1081
Lsp1109I GCAGC 3 cut(s) 35, 577, 699
LweI GCATC 1 cut(s) 457
MaeII ACGT 3 cut(s) 644, 708, 1005
MaeIII GTNAC 2 cut(s) 640, 806
MalI GATC 7 cut(s) 33, 98, 261, 483, 863, 876, 1132
MboI GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
MboII GAAGA 9 cut(s) 95, 347, 505, 625, 694, 716, 772, 947, 1007
MflI RGATCY 2 cut(s) 259, 1130
MhlI GDGCHC 3 cut(s) 411, 933, 1150
MlsI TGGCCA 1 cut(s) 740
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 2 cut(s) 410, 440
MmeI TCCRAC 2 cut(s) 237, 645
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 1 cut(s) 1107
MroNI GCCGGC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 740
MseI TTAA 2 cut(s) 200, 1167
MslI CAYNNNNRTG 2 cut(s) 54, 599
Msp20I TGGCCA 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 5 cut(s) 81, 282, 339, 623, 649
MspR9I CCNGG 3 cut(s) 82, 330, 649
Mva1269I GAATGC 3 cut(s) 452, 486, 1107
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 2 cut(s) 476, 721
NaeI GCCGGC 1 cut(s) 283
NciI CCSGG 2 cut(s) 82, 649
NdeII GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 4 cut(s) 59, 862, 1045, 1163
NlaIV GGNNCC 3 cut(s) 327, 408, 720
NmeAIII GCCGAG 2 cut(s) 750, 842
NmuCI GTSAC 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 1 cut(s) 1045
NspV TTCGAA 2 cut(s) 228, 536
OliI CACNNNNGTG 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 920
PceI AGGCCT 1 cut(s) 291
PctI GAATGC 3 cut(s) 452, 486, 1107
PdiI GCCGGC 1 cut(s) 283
PfeI GAWTC 4 cut(s) 77, 110, 230, 395
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 6 cut(s) 25, 503, 592, 654, 714, 857
PleI GAGTC 2 cut(s) 409, 439
PpsI GAGTC 2 cut(s) 409, 439
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 3 cut(s) 327, 408, 720
PspXI VCTCGAGB 1 cut(s) 920
PsuI RGATCY 2 cut(s) 259, 1130
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 2 cut(s) 54, 599
SacII CCGCGG 1 cut(s) 856
SaqAI TTAA 2 cut(s) 200, 1167
SatI GCNGC 6 cut(s) 24, 502, 591, 653, 713, 856
Sau3AI GATC 7 cut(s) 31, 96, 259, 481, 861, 874, 1130
SchI GAGTC 2 cut(s) 410, 440
ScrFI CCNGG 3 cut(s) 82, 330, 649
SduI GDGCHC 3 cut(s) 411, 933, 1150
SfaNI GCATC 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 920
Sfr303I CCGCGG 1 cut(s) 856
SfuI TTCGAA 2 cut(s) 228, 536
SgrBI CCGCGG 1 cut(s) 856
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 2 cut(s) 54, 599
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SseBI AGGCCT 1 cut(s) 291
StuI AGGCCT 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 80, 328, 647
StyI CCWWGG 2 cut(s) 27, 114
TaaI ACNGT 2 cut(s) 688, 806
TaiI ACGT 3 cut(s) 647, 711, 1008
TaqI TCGA 7 cut(s) 228, 536, 746, 801, 864, 906, 921
TauI GCSGC 3 cut(s) 504, 655, 858
TfiI GAWTC 4 cut(s) 77, 110, 230, 395
Tru1I TTAA 2 cut(s) 200, 1167
Tru9I TTAA 2 cut(s) 200, 1167
TscAI CASTG 2 cut(s) 174, 1158
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 3 cut(s) 23, 590, 712
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 6 cut(s) 44, 765, 908, 915, 1028, 1148
TspGWI ACGGA 2 cut(s) 123, 1063
TspRI CASTG 2 cut(s) 174, 1158
VneI GTGCAC 1 cut(s) 1146
XapI RAATTY 5 cut(s) 223, 238, 473, 902, 1179
XceI RCATGY 1 cut(s) 1045
XhoI CTCGAG 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 912
ZraI GACGTC 1 cut(s) 645
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.