MD13G1155200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003111
Physical Location & Seq
Reverse (-)
12098634 .. 12099848
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1155200.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGAATTGGCTGACGGGTTTGCAGCCAAGGATCAAAACAAGGTGAAGTACCTTCATGGTGGACAGATGGGATTGGATTCCGGGACTTACTTGGTGATCAATCTTCCGGATTCCAAGGCATTGCGGATTATTTGGTGCTCCGTTTTTCTGGGTTTAGTTATTCTTTCACTGCCCTGCTTTGGAACAATTCAGAGGGGATTAAAAGTTTCCCAATTGGATTTGAATTTCGAATCCGAAAATTTCAATTTCAAGCAGTTGGGTCTGCTTTTTCGTGATTTGGCCGGCGAAGGCCTGCGCAGAAAGGGTGATACATCTCTCATTGTGGCTCCTGGCAACGCCGGTACGCTTCAAAATCTTCACCCTTTTGATTTCAATGGATTTGATTTCGTTGTGGATTCGGAGTCGGTGCCGAAAAGCTCGTTCTTGGACGAGTCTATGGATTTCGTTTTCGCATTCAACTTGGCCGATGCTAAATTTGCTGATCGCATTCTGAAGATTGGCGGCATTGTTGCCGTTCCAATAAGCATCGACCCTTCGAACGCCTTTAGGCCAAAACCCAATTACAAAATCGTGTATCTCCGCCGATACGCTGCCACTTTTGTGGCAATGAGGAAGATAAGTCCGGCTTATGACTTGCCCGTGACGTCCCGGCGGCTTTGTCAGTTGGAAACCGAGGAAAAGAAGACGGTGTTAGAGGGTCTGGAAGACGTGCTGCTGGAACCGCCGAGGCTCGTTTTGGCCAAATCGAATGAATACTTGAAGAAAATCAAGTTCCTCCCCGAGTTGTTGGGTGATTCCCTCGACAGTTACAACCGCCGAGTGTTTGTCAATGTAGACCTAAACGAGGAAAACCGCGGCATGATCGAATGGTTTGATCAAAACTACCCGAAAATGAACAATGAATTCGAGGTCTACAACCTCGAGGTAGAGCCCGAAGAGGCTTCAAGCACCCCTGAAAACGACGTTTCGGACTGGTTGAAAGACAATGTGAGGGAAGAGGACTACGTTGTGATGAAAGCGGAAGCGGAAGTGGTGGTGGACATGGTGAGGAAGAGGACAATCTGTCTGGTGGACGAGCTGTTCTTGGAGTGCAGCAACGGATGGTGGCAGAGTGGGAGGAGGAATGGTGGGAGCAAGAGAGCTTATTGGGAATGCATTGCTTTGTATGGGAGGCTGAGGGATCTTGGAGTTGCTGTGCATCAGTGGTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

46.01

Weight (kDa)

5.08

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 231 - 403 7.1e-79 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 647
AbsI CCTCGAGG 1 cut(s) 920
Acc16I TGCGCA 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 406
AccI GTMKAC 2 cut(s) 834, 912
AccII CGCG 1 cut(s) 855
AccIII TCCGGA 1 cut(s) 106
AclWI GGATC 2 cut(s) 39, 1188
AcoI YGGCCR 3 cut(s) 279, 462, 738
AcsI RAATTY 4 cut(s) 223, 238, 473, 902
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 2 cut(s) 50, 343
AfiI CCNNNNNNNGG 2 cut(s) 179, 844
AgsI TTSAA 9 cut(s) 223, 244, 250, 350, 373, 457, 760, 945, 979
AhdI GACNNNNNGTC 1 cut(s) 1062
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 1 cut(s) 328
AleI CACNNNNGTG 1 cut(s) 599
AloI GAACNNNNNNTCC 2 cut(s) 1064, 1096
AluBI AGCT 3 cut(s) 417, 1078, 1142
AluI AGCT 3 cut(s) 417, 1078, 1142
Alw21I GWGCWC 1 cut(s) 140
AlwI GGATC 2 cut(s) 39, 1188
Ama87I CYCGRG 2 cut(s) 779, 920
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 5 cut(s) 279, 289, 462, 548, 738
ApeKI GCWGC 4 cut(s) 23, 590, 712, 1092
ApoI RAATTY 4 cut(s) 223, 238, 473, 902
AspLEI GCGC 1 cut(s) 297
AsuC2I CCSGG 2 cut(s) 82, 649
AsuHPI GGTGA 6 cut(s) 55, 106, 317, 350, 803, 1057
AsuII TTCGAA 2 cut(s) 228, 536
AvaI CYCGRG 2 cut(s) 779, 920
BalI TGGCCA 1 cut(s) 740
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 933
BbsI GAAGAC 2 cut(s) 689, 711
Bbv12I GWGCWC 1 cut(s) 140
BbvCI CCTCAGC 1 cut(s) 1175
BbvI GCAGC 4 cut(s) 35, 577, 699, 1104
BccI CCATC 2 cut(s) 61, 1095
BceAI ACGGC 1 cut(s) 497
BciT130I CCWGG 1 cut(s) 330
BclI TGATCA 2 cut(s) 96, 874
BcnI CCSGG 2 cut(s) 82, 649
BisI GCNGC 7 cut(s) 24, 502, 591, 653, 713, 856, 1093
BlsI GCNGC 7 cut(s) 25, 503, 592, 654, 714, 857, 1094
Bme1390I CCNGG 3 cut(s) 82, 330, 649
BmeRI GACNNNNNGTC 1 cut(s) 1062
BmeT110I CYCGRG 2 cut(s) 779, 920
BmgBI CACGTC 1 cut(s) 709
BmiI GGNNCC 3 cut(s) 327, 408, 720
BmrFI CCNGG 3 cut(s) 82, 330, 649
BmsI GCATC 3 cut(s) 457, 534, 1207
BpiI GAAGAC 2 cut(s) 689, 711
Bpu10I CCTNAGC 1 cut(s) 1175
Bpu14I TTCGAA 2 cut(s) 228, 536
BpuMI CCSGG 2 cut(s) 82, 649
BsaHI GRCGYC 1 cut(s) 644
BsaJI CCNNGG 5 cut(s) 27, 114, 672, 725, 853
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 4 cut(s) 1111, 1141, 1179, 1209
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 844
Bse118I RCCGGY 2 cut(s) 281, 338
Bse1I ACTGG 1 cut(s) 977
Bse3DI GCAATG 3 cut(s) 119, 612, 1155
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 27, 114, 672, 725, 853
BseGI GGATG 1 cut(s) 1106
BseLI CCNNNNNNNGG 2 cut(s) 179, 844
BseMI GCAATG 3 cut(s) 119, 612, 1155
BseMII CTCAG 1 cut(s) 1166
BseNI ACTGG 1 cut(s) 977
BseRI GAGGAG 1 cut(s) 1132
BseXI GCAGC 4 cut(s) 35, 577, 699, 1104
BsgI GTGCAG 1 cut(s) 1111
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 5 cut(s) 281, 291, 464, 550, 740
BshNI GGYRCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 2 cut(s) 779, 920
BsiSI CCGG 6 cut(s) 81, 107, 282, 339, 623, 649
BslFI GGGAC 2 cut(s) 97, 631
BslI CCNNNNNNNGG 2 cut(s) 179, 844
BsmFI GGGAC 2 cut(s) 97, 631
BsmI GAATGC 3 cut(s) 452, 486, 1157
BsnI GGCC 5 cut(s) 281, 291, 464, 550, 740
BsoBI CYCGRG 2 cut(s) 779, 920
Bsp119I TTCGAA 2 cut(s) 228, 536
Bsp1286I GDGCHC 2 cut(s) 140, 933
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
BspANI GGCC 5 cut(s) 281, 291, 464, 550, 740
BspCNI CTCAG 1 cut(s) 1167
BspEI TCCGGA 1 cut(s) 106
BspFNI CGCG 1 cut(s) 855
BspLI GGNNCC 3 cut(s) 327, 408, 720
BspPI GGATC 2 cut(s) 39, 1188
BspT104I TTCGAA 2 cut(s) 228, 536
BspT107I GGYRCC 1 cut(s) 406
BsrDI GCAATG 3 cut(s) 119, 612, 1155
BsrFI RCCGGY 2 cut(s) 281, 338
BsrI ACTGG 1 cut(s) 977
BssAI RCCGGY 2 cut(s) 281, 338
BssECI CCNNGG 5 cut(s) 27, 114, 672, 725, 853
BssMI GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
BssNI GRCGYC 1 cut(s) 644
BssT1I CCWWGG 2 cut(s) 27, 114
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 688, 806
Bst6I CTCTTC 3 cut(s) 930, 990, 1046
BstACI GRCGYC 1 cut(s) 644
BstBI TTCGAA 2 cut(s) 228, 536
BstC8I GCNNGC 2 cut(s) 283, 293
BstDEI CTNAG 1 cut(s) 1175
BstDSI CCRYGG 1 cut(s) 853
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 1 cut(s) 1106
BstFNI CGCG 1 cut(s) 855
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 6 cut(s) 34, 99, 484, 864, 877, 1183
BstMBI GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
BstMWI GCNNNNNNNGC 2 cut(s) 476, 721
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 3 cut(s) 80, 328, 647
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 4 cut(s) 35, 577, 699, 1104
BstV2I GAAGAC 2 cut(s) 689, 711
BstX2I RGATCY 1 cut(s) 1180
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 1180
BsuRI GGCC 5 cut(s) 281, 291, 464, 550, 740
BtgI CCRYGG 1 cut(s) 853
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 1 cut(s) 1106
BtsI GCAGTG 1 cut(s) 167
BtsIMutI CAGTG 2 cut(s) 167, 1208
Cac8I GCNNGC 2 cut(s) 283, 293
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 2 cut(s) 281, 338
Cfr42I CCGCGG 1 cut(s) 856
Csp6I GTAC 2 cut(s) 49, 342
CviAII CATG 4 cut(s) 56, 859, 1042, 1210
CviQI GTAC 2 cut(s) 49, 342
DdeI CTNAG 1 cut(s) 1175
DpnI GATC 6 cut(s) 33, 98, 483, 863, 876, 1182
DpnII GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
DriI GACNNNNNGTC 1 cut(s) 1062
EaeI YGGCCR 3 cut(s) 279, 462, 738
Eam1104I CTCTTC 3 cut(s) 930, 990, 1046
Eam1105I GACNNNNNGTC 1 cut(s) 1062
EarI CTCTTC 3 cut(s) 930, 990, 1046
EciI GGCGGA 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 27, 114
Eco147I AGGCCT 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 933
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 2 cut(s) 779, 920
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 27, 114
EcoT22I ATGCAT 1 cut(s) 1157
EcoT38I GRGCYC 1 cut(s) 933
ErhI CCWWGG 2 cut(s) 27, 114
FaeI CATG 4 cut(s) 59, 862, 1045, 1213
FaiI YATR 7 cut(s) 57, 437, 630, 860, 1043, 1167, 1211
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 2 cut(s) 97, 631
FatI CATG 4 cut(s) 55, 858, 1041, 1209
FbaI TGATCA 2 cut(s) 96, 874
FblI GTMKAC 2 cut(s) 834, 912
Fnu4HI GCNGC 7 cut(s) 24, 502, 591, 653, 713, 856, 1093
FokI GGATG 1 cut(s) 1113
FriOI GRGCYC 1 cut(s) 933
Fsp4HI GCNGC 7 cut(s) 24, 502, 591, 653, 713, 856, 1093
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 1 cut(s) 296
GluI GCNGC 7 cut(s) 24, 502, 591, 653, 713, 856, 1093
HaeIII GGCC 5 cut(s) 281, 291, 464, 550, 740
HapII CCGG 6 cut(s) 81, 107, 282, 339, 623, 649
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 644
Hin1II CATG 4 cut(s) 59, 862, 1045, 1213
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HinfI GANTC 7 cut(s) 77, 110, 230, 395, 401, 431, 794
HpaII CCGG 6 cut(s) 81, 107, 282, 339, 623, 649
HphI GGTGA 6 cut(s) 55, 106, 317, 350, 803, 1057
Hpy166II GTNNAC 5 cut(s) 62, 835, 913, 1039, 1072
Hpy188I TCNGA 5 cut(s) 192, 235, 400, 492, 970
Hpy188III TCNNGA 3 cut(s) 107, 272, 701
Hpy8I GTNNAC 5 cut(s) 62, 835, 913, 1039, 1072
Hpy99I CGWCG 1 cut(s) 965
HpyAV CCTTC 3 cut(s) 62, 281, 543
HpyCH4III ACNGT 2 cut(s) 688, 806
HpyCH4IV ACGT 4 cut(s) 644, 708, 963, 1005
HpyCH4V TGCA 4 cut(s) 23, 1092, 1155, 1198
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 721
HpyF3I CTNAG 1 cut(s) 1175
HpySE526I ACGT 4 cut(s) 644, 708, 963, 1005
Hsp92I GRCGYC 1 cut(s) 644
Hsp92II CATG 4 cut(s) 59, 862, 1045, 1213
HspAI GCGC 1 cut(s) 295
Kpn2I TCCGGA 1 cut(s) 106
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 96, 874
KspI CCGCGG 1 cut(s) 856
Kzo9I GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
LmnI GCTCC 3 cut(s) 143, 331, 1131
Lsp1109I GCAGC 4 cut(s) 35, 577, 699, 1104
LweI GCATC 3 cut(s) 457, 534, 1207
MaeII ACGT 4 cut(s) 644, 708, 963, 1005
MaeIII GTNAC 2 cut(s) 640, 806
MalI GATC 6 cut(s) 33, 98, 483, 863, 876, 1182
MboI GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 1 cut(s) 1180
MhlI GDGCHC 2 cut(s) 140, 933
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 9 cut(s) 5, 186, 212, 223, 238, 244, 473, 559, 902
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 2 cut(s) 410, 440
MmeI TCCRAC 1 cut(s) 645
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 1 cut(s) 1157
MroI TCCGGA 1 cut(s) 106
MroNI GCCGGC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 740
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 599
Msp20I TGGCCA 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 6 cut(s) 81, 107, 282, 339, 623, 649
MspR9I CCNGG 3 cut(s) 82, 330, 649
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 3 cut(s) 452, 486, 1157
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 2 cut(s) 476, 721
NaeI GCCGGC 1 cut(s) 283
NciI CCSGG 2 cut(s) 82, 649
NdeII GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 4 cut(s) 59, 862, 1045, 1213
NlaIV GGNNCC 3 cut(s) 327, 408, 720
NmeAIII GCCGAG 2 cut(s) 750, 842
NmuCI GTSAC 1 cut(s) 640
NsbI TGCGCA 1 cut(s) 296
NsiI ATGCAT 1 cut(s) 1157
NspV TTCGAA 2 cut(s) 228, 536
OliI CACNNNNGTG 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 920
PceI AGGCCT 1 cut(s) 291
PctI GAATGC 3 cut(s) 452, 486, 1157
PdiI GCCGGC 1 cut(s) 283
PfeI GAWTC 5 cut(s) 77, 110, 230, 395, 794
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 7 cut(s) 25, 503, 592, 654, 714, 857, 1094
PleI GAGTC 2 cut(s) 409, 439
PpsI GAGTC 2 cut(s) 409, 439
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 3 cut(s) 327, 408, 720
PspXI VCTCGAGB 1 cut(s) 920
PsuI RGATCY 1 cut(s) 1180
RsaI GTAC 2 cut(s) 50, 343
RsaNI GTAC 2 cut(s) 49, 342
RseI CAYNNNNRTG 1 cut(s) 599
SacII CCGCGG 1 cut(s) 856
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 7 cut(s) 24, 502, 591, 653, 713, 856, 1093
Sau3AI GATC 6 cut(s) 31, 96, 481, 861, 874, 1180
SchI GAGTC 2 cut(s) 410, 440
ScrFI CCNGG 3 cut(s) 82, 330, 649
SduI GDGCHC 2 cut(s) 140, 933
SfaNI GCATC 3 cut(s) 457, 534, 1207
Sfr274I CTCGAG 1 cut(s) 920
Sfr303I CCGCGG 1 cut(s) 856
SfuI TTCGAA 2 cut(s) 228, 536
SgrBI CCGCGG 1 cut(s) 856
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 9 cut(s) 5, 186, 212, 223, 238, 244, 473, 559, 902
SseBI AGGCCT 1 cut(s) 291
StuI AGGCCT 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 80, 328, 647
StyI CCWWGG 2 cut(s) 27, 114
TaaI ACNGT 2 cut(s) 688, 806
TaiI ACGT 4 cut(s) 647, 711, 966, 1008
TaqI TCGA 8 cut(s) 228, 528, 536, 746, 801, 864, 906, 921
TasI AATT 9 cut(s) 5, 186, 212, 223, 238, 244, 473, 559, 902
TauI GCSGC 3 cut(s) 504, 655, 858
TfiI GAWTC 5 cut(s) 77, 110, 230, 395, 794
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 2 cut(s) 174, 1208
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 4 cut(s) 23, 590, 712, 1092
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 6 cut(s) 44, 765, 908, 915, 1028, 1198
TspGWI ACGGA 2 cut(s) 130, 1113
TspRI CASTG 2 cut(s) 174, 1208
XagI CCTNNNNNAGG 1 cut(s) 842
XapI RAATTY 4 cut(s) 223, 238, 473, 902
XhoI CTCGAG 1 cut(s) 920
XmiI GTMKAC 2 cut(s) 834, 912
ZraI GACGTC 1 cut(s) 645
Zsp2I ATGCAT 1 cut(s) 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.