Rmu_co8066912.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8066912.1
OG0003111
Physical Location & Seq
Forward (+)
13 .. 573
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8066912.1_g000001.1.cds

Sequence Viewer

Length: 561 bp
atgaggaagaccggtccggctggtgagctgcaggagaatcctggccgccggcggctttgccaatttggaagtggggccaaggagaccgtcctgcagggtcttgaagatgtgttgcttgagccaccaagacgtgctttggcaaaatcaagccagtacttgaagaagatgaagttcctccctgacctgttggggaattctctagaaaattacgagcgccgtgtgtttgttaatgtggacttgaatgagaaaaaaggtggcgcaattgaatggtttcatcaaaactatccgaaaatgaatacggattttgaagtacacaaccttgaaatagaagaacaacaagacgaagaagcttcaagcaagccgcagactgatgtttcaggttggctaaaggagaatgtgaaggaagaggactatgttgtgatgaaagcagaagcagaagttgttgtggatatgatgcagaagagcacaatctgtctggtggatgagttgtttttggagtgtaacaatgaatggtggcagaatggaaagagtgacaagagcaagagagcttattgggagtgc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

21.69

Weight (kDa)

5.01

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 46, 52, 362
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 1 cut(s) 193
AfaI GTAC 2 cut(s) 155, 312
AgeI ACCGGT 1 cut(s) 11
AgsI TTSAA 7 cut(s) 104, 160, 241, 266, 308, 323, 354
AjiI CACGTC 1 cut(s) 131
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 3 cut(s) 28, 350, 548
AluI AGCT 3 cut(s) 28, 350, 548
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 1 cut(s) 77
AoxI GGCC 2 cut(s) 43, 75
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 193
AsiGI ACCGGT 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 2 cut(s) 216, 260
AspS9I GGNCC 2 cut(s) 14, 75
AsuHPI GGTGA 1 cut(s) 35
AvaII GGWCC 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 1 cut(s) 15
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 2 cut(s) 29, 92
BfoI RGCGCY 1 cut(s) 217
BisI GCNGC 4 cut(s) 29, 46, 53, 362
BlsI GCNGC 4 cut(s) 30, 47, 54, 363
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 14
BmgBI CACGTC 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 14, 75
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 444
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 11
Bse118I RCCGGY 2 cut(s) 11, 48
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 487
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 15
BshFI GGCC 2 cut(s) 45, 77
BshTI ACCGGT 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 467
BsiSI CCGG 3 cut(s) 12, 17, 49
BsmAI GTCTC 1 cut(s) 77
BsnI GGCC 2 cut(s) 45, 77
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 467
BspACI CCGC 3 cut(s) 46, 52, 362
BspANI GGCC 2 cut(s) 45, 77
BspLI GGNNCC 1 cut(s) 76
BspMAI CTGCAG 2 cut(s) 33, 96
BspQI GCTCTTC 1 cut(s) 455
BspTNI GGTCTC 1 cut(s) 77
BsrFI RCCGGY 2 cut(s) 11, 48
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 2 cut(s) 11, 48
BssECI CCNNGG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 2 cut(s) 399, 455
BstC8I GCNNGC 2 cut(s) 50, 359
BstF5I GGATG 1 cut(s) 487
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 2 cut(s) 216, 260
BstMAI GTCTC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 2 cut(s) 29, 92
BstV1I GCAGC 1 cut(s) 15
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 45, 77
BtrI CACGTC 1 cut(s) 131
BtsCI GGATG 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 50, 359
CfoI GCGC 2 cut(s) 216, 260
Cfr10I RCCGGY 2 cut(s) 11, 48
Cfr13I GGNCC 2 cut(s) 14, 75
CpoI CGGWCCG 1 cut(s) 14
Csp6I GTAC 2 cut(s) 154, 311
CspAI ACCGGT 1 cut(s) 11
CspI CGGWCCG 1 cut(s) 14
CviQI GTAC 2 cut(s) 154, 311
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 2 cut(s) 399, 455
EarI CTCTTC 2 cut(s) 399, 455
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 14
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaiI YATR 2 cut(s) 414, 452
FalI AAGNNNNNCTT 2 cut(s) 118, 150
Fnu4HI GCNGC 4 cut(s) 29, 46, 53, 362
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 4 cut(s) 29, 46, 53, 362
FspBI CTAG 1 cut(s) 200
GlaI GCGC 2 cut(s) 215, 259
GluI GCNGC 4 cut(s) 29, 46, 53, 362
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 2 cut(s) 45, 77
HapII CCGG 3 cut(s) 12, 17, 49
HhaI GCGC 2 cut(s) 216, 260
Hin6I GCGC 2 cut(s) 214, 258
HinP1I GCGC 2 cut(s) 214, 258
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 1 cut(s) 37
HpaII CCGG 3 cut(s) 12, 17, 49
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 2 cut(s) 235, 313
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 2 cut(s) 101, 200
Hpy8I GTNNAC 2 cut(s) 235, 313
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 3 cut(s) 31, 94, 457
HpySE526I ACGT 1 cut(s) 130
HspAI GCGC 2 cut(s) 214, 258
KroI GCCGGC 1 cut(s) 48
KroNI GCCGGC 1 cut(s) 50
LguI GCTCTTC 1 cut(s) 455
Lsp1109I GCAGC 1 cut(s) 15
LweI GCATC 1 cut(s) 444
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 500, 530
MboII GAAGA 8 cut(s) 19, 116, 172, 175, 341, 356, 416, 472
MfeI CAATTG 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 4 cut(s) 62, 193, 205, 261
MnlI CCTC 2 cut(s) 185, 400
MreI CGCCGGCG 1 cut(s) 48
MroNI GCCGGC 1 cut(s) 48
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 1 cut(s) 228
MspI CCGG 3 cut(s) 12, 17, 49
MspR9I CCNGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 42
NaeI GCCGGC 1 cut(s) 50
NgoMIV GCCGGC 1 cut(s) 48
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 530
PciSI GCTCTTC 1 cut(s) 455
PdiI GCCGGC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 4 cut(s) 30, 47, 54, 363
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 2 cut(s) 14, 75
PstI CTGCAG 2 cut(s) 33, 96
RsaI GTAC 2 cut(s) 155, 312
RsaNI GTAC 2 cut(s) 154, 311
Rsr2I CGGWCCG 1 cut(s) 14
RsrII CGGWCCG 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 29, 46, 53, 362
Sau96I GGNCC 2 cut(s) 14, 75
SbfI CCTGCAGG 1 cut(s) 96
ScaI AGTACT 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 42
SdaI CCTGCAGG 1 cut(s) 96
SduI GDGCHC 1 cut(s) 467
SetI ASST 8 cut(s) 30, 133, 186, 256, 321, 352, 382, 550
SfaNI GCATC 1 cut(s) 444
SfcI CTRYAG 2 cut(s) 29, 92
SgrAI CRCCGGYG 1 cut(s) 48
SinI GGWCC 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse8387I CCTGCAGG 1 cut(s) 96
Sse9I AATT 4 cut(s) 62, 193, 205, 261
SsiI CCGC 3 cut(s) 46, 52, 362
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 133
TasI AATT 4 cut(s) 62, 193, 205, 261
TatI WGTACW 2 cut(s) 153, 310
TauI GCSGC 3 cut(s) 48, 55, 364
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 5 cut(s) 182, 263, 308, 437, 522
TspGWI ACGGA 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 1 cut(s) 193
XbaI TCTAGA 1 cut(s) 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.