FvH4_4g26480

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004230
Physical Location & Seq
Forward (+)
27872183 .. 27873836
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26480.t1

Sequence Viewer

Length: 972 bp
ATGAGGAGTCTGAGGAAAGAAGAATCAGCAGCTGCGTTAACCATTCTCCTCTATTCTGTGCTGACTTCTCTATTTTTCCTTGCAGTTCAAGCAAGAAGTCATAAACAGGCATGTCGTTCTTCGTGTGGAGGTATTGAAAACATCAGCTATCCTTTTCGCCTAAAGGGTGATCCGGTAGGCTGCGGTGATCCTGACTATGAGCTATCATGTGTTAACAACAAAACCATCCTAGAAATATTTCCAGGAAAATACTATGTAAAGAACATTTCTTATGATGAGAATGTTATCCGTTTGGTCGATGTCAACTTTGCCAATGGTAGCAGCTGCAGCCTTCCATCAGGATCTGTTGTAACGAGGAATGGAGATGTCAAGGATTTGCGCTTTGTGAGGCTTGGCGCCGAGCGTACTTACACACAGTTTGTGAAGTGCTCCAGAAACATCAGCCAGGCTGATGTAACAGCTTTTAACTACACAGCCGTTCCATGTTTGACTGGAGTTAATGGGACTAATTACTTCTATGCTGTTTATGATGGTGACTACTTAACTGGTCATCGTAAACCACCATGCTCGCTTATTTCAGTGGCTTCATCAGATAATCATGAAAATCTTGTTAAGCCCCTTTCTTATGAAGCCATCATGAAGCACTTGGAGGCAGGGTTTGATCTCGCATGGTCCGTTGAGTGTCGCGATTGTTCTTTAGCTGGCAAATATTGTGGAGTAGATGTAAGTGATCTGAATTTGGGTACTAAGCCACTCCCCTATCAATGTTCTGAAGATTCAGAGTACAAAGAACTCACAGAGCTCCAAATGATCTTAATATATGTGGGAATTTCTTTGGGAGCTCTATTTGCTTTTGTGCTTATAGTGGTTTTATTGGTGTTACGCATCCGCAAATGTATGAGACAAAGACAGCGGGAGAGGAATCTGCAGAATCAGCAGCAGTCATCGACACCAGCACTCCACAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

324

Amino Acids

35.95

Weight (kDa)

7.89

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 100 1.3e-19 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 395
AccII CGCG 1 cut(s) 687
AciI CCGC 3 cut(s) 183, 889, 913
AclWI GGATC 3 cut(s) 164, 182, 349
AcsI RAATTY 2 cut(s) 736, 828
AcuI CTGAAG 1 cut(s) 792
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 3 cut(s) 406, 745, 785
AgsI TTSAA 2 cut(s) 89, 137
AjnI CCWGG 2 cut(s) 241, 444
AluBI AGCT 8 cut(s) 32, 147, 202, 324, 461, 701, 802, 842
AluI AGCT 8 cut(s) 32, 147, 202, 324, 461, 701, 802, 842
Alw21I GWGCWC 3 cut(s) 431, 804, 844
Alw26I GTCTC 1 cut(s) 895
AlwI GGATC 3 cut(s) 164, 182, 349
AlwNI CAGNNNCTG 2 cut(s) 32, 344
ApeKI GCWGC 7 cut(s) 29, 32, 180, 321, 324, 327, 937
ApoI RAATTY 2 cut(s) 736, 828
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 2 cut(s) 381, 398
AspS9I GGNCC 1 cut(s) 672
AsuHPI GGTGA 3 cut(s) 179, 197, 545
AvaII GGWCC 1 cut(s) 672
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 2 cut(s) 804, 844
Bbv12I GWGCWC 3 cut(s) 431, 804, 844
BbvI GCAGC 7 cut(s) 19, 41, 167, 311, 333, 339, 949
BccI CCATC 4 cut(s) 233, 343, 524, 641
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 2 cut(s) 243, 446
BcoDI GTCTC 1 cut(s) 895
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 2 cut(s) 325, 926
BfoI RGCGCY 1 cut(s) 399
BisI GCNGC 7 cut(s) 30, 33, 181, 322, 325, 328, 938
BlsI GCNGC 7 cut(s) 31, 34, 182, 323, 326, 329, 939
Bme1390I CCNGG 2 cut(s) 243, 446
Bme18I GGWCC 1 cut(s) 672
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 243, 446
BmsI GCATC 1 cut(s) 894
BpmI CTGGAG 2 cut(s) 415, 513
BsaHI GRCGYC 1 cut(s) 396
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 6 cut(s) 641, 671, 708, 738, 942, 972
Bse1I ACTGG 2 cut(s) 496, 550
BseBI CCWGG 2 cut(s) 243, 446
BseGI GGATG 2 cut(s) 225, 885
BseNI ACTGG 2 cut(s) 496, 550
BseRI GAGGAG 2 cut(s) 19, 38
BseXI GCAGC 7 cut(s) 19, 41, 167, 311, 333, 339, 949
Bsh1236I CGCG 1 cut(s) 687
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 3 cut(s) 431, 804, 844
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 517
BsmAI GTCTC 1 cut(s) 895
BsmFI GGGAC 1 cut(s) 517
Bsp1286I GDGCHC 3 cut(s) 431, 804, 844
Bsp143I GATC 6 cut(s) 169, 187, 341, 661, 730, 810
Bsp68I TCGCGA 1 cut(s) 687
BspACI CCGC 3 cut(s) 183, 889, 913
BspFNI CGCG 1 cut(s) 687
BspHI TCATGA 2 cut(s) 598, 636
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 2 cut(s) 329, 930
BspPI GGATC 3 cut(s) 164, 182, 349
BspT107I GGYRCC 1 cut(s) 395
BsrI ACTGG 2 cut(s) 496, 550
BssMI GATC 6 cut(s) 169, 187, 341, 661, 730, 810
BssNI GRCGYC 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 243, 446
Bst4CI ACNGT 2 cut(s) 417, 965
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 2 cut(s) 569, 703
BstDEI CTNAG 2 cut(s) 11, 747
BstF5I GGATG 2 cut(s) 225, 885
BstFNI CGCG 1 cut(s) 687
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 381, 398
BstKTI GATC 6 cut(s) 172, 190, 344, 664, 733, 813
BstMAI GTCTC 1 cut(s) 895
BstMBI GATC 6 cut(s) 169, 187, 341, 661, 730, 810
BstMWI GCNNNNNNNGC 4 cut(s) 89, 327, 848, 934
BstNI CCWGG 2 cut(s) 243, 446
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 2 cut(s) 241, 444
BstSFI CTRYAG 2 cut(s) 325, 926
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 7 cut(s) 19, 41, 167, 311, 333, 339, 949
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
BtsCI GGATG 2 cut(s) 225, 885
BtsIMutI CAGTG 1 cut(s) 585
BtuMI TCGCGA 1 cut(s) 687
Cac8I GCNNGC 2 cut(s) 569, 703
CaiI CAGNNNCTG 2 cut(s) 32, 344
CciI TCATGA 2 cut(s) 598, 636
CfoI GCGC 2 cut(s) 381, 398
Cfr13I GGNCC 1 cut(s) 672
Csp6I GTAC 3 cut(s) 405, 744, 784
CspCI CAANNNNNGTGG 3 cut(s) 694, 729, 950
CviAII CATG 7 cut(s) 111, 207, 483, 564, 599, 637, 669
CviQI GTAC 3 cut(s) 405, 744, 784
DdeI CTNAG 2 cut(s) 11, 747
DinI GGCGCC 1 cut(s) 397
DpnI GATC 6 cut(s) 171, 189, 343, 663, 732, 812
DpnII GATC 6 cut(s) 169, 187, 341, 661, 730, 810
Ecl136II GAGCTC 2 cut(s) 802, 842
Eco24I GRGCYC 2 cut(s) 804, 844
Eco47I GGWCC 1 cut(s) 672
Eco53kI GAGCTC 2 cut(s) 802, 842
Eco57I CTGAAG 1 cut(s) 792
EcoICRI GAGCTC 2 cut(s) 802, 842
EcoRII CCWGG 2 cut(s) 241, 444
EcoT38I GRGCYC 2 cut(s) 804, 844
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
FaeI CATG 7 cut(s) 114, 210, 486, 567, 602, 640, 672
FaqI GGGAC 1 cut(s) 517
FatI CATG 7 cut(s) 110, 206, 482, 563, 598, 636, 668
FauI CCCGC 1 cut(s) 906
Fnu4HI GCNGC 7 cut(s) 30, 33, 181, 322, 325, 328, 938
FokI GGATG 2 cut(s) 212, 872
FriOI GRGCYC 2 cut(s) 804, 844
Fsp4HI GCNGC 7 cut(s) 30, 33, 181, 322, 325, 328, 938
FspBI CTAG 1 cut(s) 230
GlaI GCGC 2 cut(s) 380, 397
GluI GCNGC 7 cut(s) 30, 33, 181, 322, 325, 328, 938
GsuI CTGGAG 2 cut(s) 415, 513
HaeII RGCGCY 1 cut(s) 399
HapII CCGG 1 cut(s) 173
HhaI GCGC 2 cut(s) 381, 398
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 7 cut(s) 114, 210, 486, 567, 602, 640, 672
Hin6I GCGC 2 cut(s) 379, 396
HinP1I GCGC 2 cut(s) 379, 396
HincII GTYRAC 3 cut(s) 39, 214, 304
HindII GTYRAC 3 cut(s) 39, 214, 304
HinfI GANTC 5 cut(s) 7, 23, 776, 922, 931
HpaI GTTAAC 2 cut(s) 39, 214
HpaII CCGG 1 cut(s) 173
HphI GGTGA 3 cut(s) 179, 197, 545
Hpy166II GTNNAC 4 cut(s) 39, 214, 304, 557
Hpy188I TCNGA 5 cut(s) 12, 592, 735, 772, 781
Hpy188III TCNNGA 7 cut(s) 191, 339, 432, 599, 637, 686, 969
Hpy8I GTNNAC 4 cut(s) 39, 214, 304, 557
HpyAV CCTTC 1 cut(s) 341
HpyCH4III ACNGT 2 cut(s) 417, 965
HpyCH4V TGCA 3 cut(s) 83, 327, 928
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 327, 848, 934
HpyF3I CTNAG 2 cut(s) 11, 747
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 7 cut(s) 114, 210, 486, 567, 602, 640, 672
HspAI GCGC 2 cut(s) 379, 396
KasI GGCGCC 1 cut(s) 395
KspAI GTTAAC 2 cut(s) 39, 214
Kzo9I GATC 6 cut(s) 169, 187, 341, 661, 730, 810
LmnI GCTCC 3 cut(s) 434, 807, 839
Lsp1109I GCAGC 7 cut(s) 19, 41, 167, 311, 333, 339, 949
LweI GCATC 1 cut(s) 894
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 4 cut(s) 349, 454, 533, 879
MalI GATC 6 cut(s) 171, 189, 343, 663, 732, 812
MboI GATC 6 cut(s) 169, 187, 341, 661, 730, 810
MboII GAAGA 3 cut(s) 32, 111, 785
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 3 cut(s) 431, 804, 844
MluCI AATT 3 cut(s) 508, 736, 828
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 7 cut(s) 6, 59, 122, 348, 381, 643, 912
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 7 cut(s) 38, 213, 465, 498, 542, 612, 815
MspA1I CMGCKG 3 cut(s) 32, 324, 913
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 2 cut(s) 243, 446
MvaI CCWGG 2 cut(s) 243, 446
MvnI CGCG 1 cut(s) 687
MwoI GCNNNNNNNGC 4 cut(s) 89, 327, 848, 934
NarI GGCGCC 1 cut(s) 396
NdeII GATC 6 cut(s) 169, 187, 341, 661, 730, 810
NlaIII CATG 7 cut(s) 114, 210, 486, 567, 602, 640, 672
NlaIV GGNNCC 1 cut(s) 397
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 533
NruI TCGCGA 1 cut(s) 687
NspI RCATGY 1 cut(s) 114
PagI TCATGA 2 cut(s) 598, 636
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 4 cut(s) 23, 776, 922, 931
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 7 cut(s) 31, 34, 182, 323, 326, 329, 939
PleI GAGTC 1 cut(s) 15
PluTI GGCGCC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 15
Psp124BI GAGCTC 2 cut(s) 804, 844
Psp6I CCWGG 2 cut(s) 241, 444
PspGI CCWGG 2 cut(s) 241, 444
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 1 cut(s) 672
PstI CTGCAG 2 cut(s) 329, 930
PstNI CAGNNNCTG 2 cut(s) 32, 344
PsuI RGATCY 1 cut(s) 341
PvuII CAGCTG 2 cut(s) 32, 324
RruI TCGCGA 1 cut(s) 687
RsaI GTAC 3 cut(s) 406, 745, 785
RsaNI GTAC 3 cut(s) 405, 744, 784
SacI GAGCTC 2 cut(s) 804, 844
SaqAI TTAA 7 cut(s) 38, 213, 465, 498, 542, 612, 815
SatI GCNGC 7 cut(s) 30, 33, 181, 322, 325, 328, 938
Sau3AI GATC 6 cut(s) 169, 187, 341, 661, 730, 810
Sau96I GGNCC 1 cut(s) 672
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 243, 446
SduI GDGCHC 3 cut(s) 431, 804, 844
SetI ASST 9 cut(s) 34, 133, 149, 204, 326, 463, 703, 804, 844
SfaNI GCATC 1 cut(s) 894
SfcI CTRYAG 2 cut(s) 325, 926
SfoI GGCGCC 1 cut(s) 397
SinI GGWCC 1 cut(s) 672
Sse9I AATT 3 cut(s) 508, 736, 828
SsiI CCGC 3 cut(s) 183, 889, 913
SspDI GGCGCC 1 cut(s) 395
SspI AATATT 2 cut(s) 237, 710
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 2 cut(s) 804, 844
StyD4I CCNGG 2 cut(s) 241, 444
TaaI ACNGT 2 cut(s) 417, 965
TaqI TCGA 2 cut(s) 297, 947
TasI AATT 3 cut(s) 508, 736, 828
TatI WGTACW 1 cut(s) 783
TfiI GAWTC 4 cut(s) 23, 776, 922, 931
Tru1I TTAA 7 cut(s) 38, 213, 465, 498, 542, 612, 815
Tru9I TTAA 7 cut(s) 38, 213, 465, 498, 542, 612, 815
TscAI CASTG 1 cut(s) 585
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 7 cut(s) 29, 32, 180, 321, 324, 327, 937
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 4 cut(s) 576, 615, 642, 653
TspGWI ACGGA 2 cut(s) 278, 664
TspRI CASTG 1 cut(s) 585
VpaK11BI GGWCC 1 cut(s) 672
XapI RAATTY 2 cut(s) 736, 828
XceI RCATGY 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.