Rroxscaffold_5G00375530

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004230
Physical Location & Seq
Forward (+)
56332156 .. 56334347
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375530.1

Sequence Viewer

Length: 1095 bp
ATGAGGAAAGCCTCAACATCAATTGCATCAACCGTTCCTTTTCCTGTCCTAGTTTTTCTACTCTTCCTTGTTCAAGTTCAAGCAAGAAACTTGAATGAAGCTGATTGTTCTTCTTCCTGTGGAGATATCCACAACATCAAGTACCCTTTCCGCCTTAAAGGCGATCCATCCGGCTGCGGTGACCCCGACTTTGAATTCTCATGTGTCATCAACAAAACCATCCTCGAAATATTTCCGGTTGACGTCAACTTCGCAAACGGAAGCTGCAGCCTCCCATCTGGTTCTATAGAAAATTCAGATGGTTTCGTCCAAGACGTTCGTTACAAGGGTCATGTCAACTACACAACTGGATCTCGTATCCGATTTGTCAAGTGCTCAAGAAATGTCACCCTGCAGAATAATGAGAAGTATTATTATAACTACACGGAGGTTCCTTGTTTGACAAGAAATGGAACTTACACGTATACTGTTCTAGATGGAATGTACACGGCTATGGATTATCCACCGACGTGCTCACTTGTTTCGCTGGCTCCTGTGGATTATCATGAAGATGTTTATAAGTCCCCTACTTATGAAGCCATAGTGAAAGTGTTGCAGGCAGGGTTTGATCTCGGTTGGTCTGTTGAGTGCAGGGATTGCTCTTTGGCTGGTAAGCATTGCGTAATCGATGACAAAGCATGGGACACTAGGCCTATCATATTCCAATGTGCAAAAGATTACAAAGAACTCACACAGTTTGAAGCAAGATTGATCATTGCGGGAATCTGTGTGGCAGGTGTAATTGGTCTATTGCTTATAGTTGCTGTATTGGTGTTTGTTATTCGCAAATATAAGAAACGAAACAAGCCCTTAGTTGGAGATTCGGAGACTTGGGAATCCAATGTGTATAGTGGGGTTATGGTGCGTCAAGTCAAGGTATCCGATCATCCCTCGATTACAGAAGTTGTGGAGATGTTGAAAGGAAACATCGATAGCCTCCAAATGCCTCCTCATCCTTCTTATCATCAGCATCTTTCCACCTCTACAGAGGCAGAGGACCAATTGGATCCTTCTGATGATCAGATGCTGATTTCAAAGACAATGCAAAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

364

Amino Acids

40.75

Weight (kDa)

5.65

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 79 1.3e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 417, 558
AarI CACCTGC 1 cut(s) 764
AatII GACGTC 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 764
AccI GTMKAC 1 cut(s) 464
AciI CCGC 3 cut(s) 151, 177, 758
AclWI GGATC 4 cut(s) 158, 358, 1040, 1053
AcsI RAATTY 2 cut(s) 194, 292
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 143, 485
AfiI CCNNNNNNNGG 1 cut(s) 854
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 7 cut(s) 74, 80, 94, 194, 740, 958, 1074
AjiI CACGTC 1 cut(s) 510
AleI CACNNNNGTG 1 cut(s) 508
AluBI AGCT 3 cut(s) 101, 264, 1092
AluI AGCT 3 cut(s) 101, 264, 1092
Alw21I GWGCWC 2 cut(s) 377, 515
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 4 cut(s) 158, 358, 1040, 1053
AlwNI CAGNNNCTG 1 cut(s) 1066
AoxI GGCC 1 cut(s) 689
ApeKI GCWGC 3 cut(s) 174, 264, 267
ApoI RAATTY 2 cut(s) 194, 292
ArsI GACNNNNNNTTYG 4 cut(s) 173, 205, 233, 265
Asp700I GAANNNNTTC 1 cut(s) 231
AspS9I GGNCC 1 cut(s) 1036
AsuHPI GGTGA 2 cut(s) 191, 379
AvaII GGWCC 1 cut(s) 1036
BamHI GGATCC 1 cut(s) 1045
Bbv12I GWGCWC 2 cut(s) 377, 515
BbvI GCAGC 3 cut(s) 161, 251, 279
BccI CCATC 5 cut(s) 175, 227, 283, 293, 470
BceAI ACGGC 1 cut(s) 504
BciVI GTATCC 2 cut(s) 368, 928
BclI TGATCA 2 cut(s) 750, 1057
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 4 cut(s) 50, 473, 687, 1093
BfmI CTRYAG 4 cut(s) 265, 285, 392, 1023
BfuAI ACCTGC 1 cut(s) 764
BfuI GTATCC 2 cut(s) 368, 928
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 3 cut(s) 175, 265, 268
BlsI GCNGC 3 cut(s) 176, 266, 269
Bme18I GGWCC 1 cut(s) 1036
BmgBI CACGTC 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 1036
BmiI GGNNCC 3 cut(s) 432, 531, 1047
BmsI GCATC 3 cut(s) 35, 1018, 1053
BpuEI CTTGAG 1 cut(s) 361
Bsa29I ATCGAT 2 cut(s) 666, 969
BsaAI YACGTR 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 243
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 1 cut(s) 854
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 2 cut(s) 655, 753
BseCI ATCGAT 2 cut(s) 666, 969
BseGI GGATG 4 cut(s) 167, 219, 925, 991
BseLI CCNNNNNNNGG 1 cut(s) 854
BseMI GCAATG 2 cut(s) 655, 753
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 978
BseXI GCAGC 3 cut(s) 161, 251, 279
BsgI GTGCAG 1 cut(s) 649
BshFI GGCC 1 cut(s) 691
BshVI ATCGAT 2 cut(s) 666, 969
BsiHKAI GWGCWC 2 cut(s) 377, 515
BsiSI CCGG 2 cut(s) 171, 236
BslFI GGGAC 2 cut(s) 547, 695
BslI CCNNNNNNNGG 1 cut(s) 854
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 2 cut(s) 547, 695
BsnI GGCC 1 cut(s) 691
Bsp1286I GDGCHC 2 cut(s) 377, 515
Bsp1407I TGTACA 1 cut(s) 483
Bsp143I GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
BspACI CCGC 3 cut(s) 151, 177, 758
BspANI GGCC 1 cut(s) 691
BspDI ATCGAT 2 cut(s) 666, 969
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 3 cut(s) 432, 531, 1047
BspMAI CTGCAG 2 cut(s) 269, 396
BspMI ACCTGC 1 cut(s) 764
BspPI GGATC 4 cut(s) 158, 358, 1040, 1053
BsrDI GCAATG 2 cut(s) 655, 753
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 1 cut(s) 352
BssMI GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
BssNAI GTATAC 1 cut(s) 465
BssNI GRCGYC 1 cut(s) 243
Bst1107I GTATAC 1 cut(s) 465
Bst4CI ACNGT 3 cut(s) 34, 469, 735
Bst6I CTCTTC 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 243
BstAPI GCANNNNNTGC 1 cut(s) 636
BstAUI TGTACA 1 cut(s) 483
BstBAI YACGTR 1 cut(s) 462
BstC8I GCNNGC 2 cut(s) 528, 597
BstDEI CTNAG 1 cut(s) 850
BstEII GGTNACC 1 cut(s) 179
BstF5I GGATG 4 cut(s) 167, 219, 925, 991
BstKTI GATC 7 cut(s) 166, 353, 610, 753, 925, 1048, 1060
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 159, 636
BstPI GGTNACC 1 cut(s) 179
BstSFI CTRYAG 4 cut(s) 265, 285, 392, 1023
BstV1I GCAGC 3 cut(s) 161, 251, 279
BstX2I RGATCY 2 cut(s) 350, 1045
BstYI RGATCY 2 cut(s) 350, 1045
BstZ17I GTATAC 1 cut(s) 465
Bsu15I ATCGAT 2 cut(s) 666, 969
BsuI GTATCC 2 cut(s) 368, 928
BsuRI GGCC 1 cut(s) 691
BsuTUI ATCGAT 2 cut(s) 666, 969
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 4 cut(s) 167, 219, 925, 991
BveI ACCTGC 1 cut(s) 764
Cac8I GCNNGC 2 cut(s) 528, 597
CaiI CAGNNNCTG 1 cut(s) 1066
CciI TCATGA 1 cut(s) 544
Cfr13I GGNCC 1 cut(s) 1036
ClaI ATCGAT 2 cut(s) 666, 969
CseI GACGC 1 cut(s) 893
Csp6I GTAC 2 cut(s) 142, 484
CviAII CATG 4 cut(s) 201, 332, 545, 678
CviQI GTAC 2 cut(s) 142, 484
DdeI CTNAG 1 cut(s) 850
DpnI GATC 7 cut(s) 165, 352, 609, 752, 924, 1047, 1059
DpnII GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 140
Eco147I AGGCCT 1 cut(s) 691
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 1036
Eco91I GGTNACC 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 194
EcoRV GATATC 1 cut(s) 127
FaeI CATG 4 cut(s) 204, 335, 548, 681
FaqI GGGAC 2 cut(s) 547, 695
FatI CATG 4 cut(s) 200, 331, 544, 677
FauI CCCGC 1 cut(s) 751
FbaI TGATCA 2 cut(s) 750, 1057
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 3 cut(s) 175, 265, 268
FokI GGATG 4 cut(s) 154, 206, 912, 978
Fsp4HI GCNGC 3 cut(s) 175, 265, 268
FspBI CTAG 4 cut(s) 50, 473, 687, 1093
GluI GCNGC 3 cut(s) 175, 265, 268
HaeIII GGCC 1 cut(s) 691
HapII CCGG 2 cut(s) 171, 236
HgaI GACGC 1 cut(s) 893
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 4 cut(s) 204, 335, 548, 681
HincII GTYRAC 3 cut(s) 241, 247, 337
HindII GTYRAC 3 cut(s) 241, 247, 337
HinfI GANTC 3 cut(s) 762, 860, 875
HpaII CCGG 2 cut(s) 171, 236
HphI GGTGA 2 cut(s) 191, 379
Hpy166II GTNNAC 5 cut(s) 241, 247, 337, 465, 486
Hpy188I TCNGA 6 cut(s) 298, 362, 865, 922, 1054, 1062
Hpy188III TCNNGA 3 cut(s) 378, 473, 545
Hpy8I GTNNAC 5 cut(s) 241, 247, 337, 465, 486
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 1005, 1059
HpyCH4III ACNGT 3 cut(s) 34, 469, 735
HpyCH4IV ACGT 4 cut(s) 243, 315, 461, 509
HpyCH4V TGCA 7 cut(s) 26, 267, 394, 595, 630, 710, 1084
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 636
HpyF3I CTNAG 1 cut(s) 850
HpySE526I ACGT 4 cut(s) 243, 315, 461, 509
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 4 cut(s) 204, 335, 548, 681
Ksp22I TGATCA 2 cut(s) 750, 1057
Kzo9I GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
LmnI GCTCC 1 cut(s) 535
Lsp1109I GCAGC 3 cut(s) 161, 251, 279
LweI GCATC 3 cut(s) 35, 1018, 1053
MaeI CTAG 4 cut(s) 50, 473, 687, 1093
MaeII ACGT 4 cut(s) 243, 315, 461, 509
MaeIII GTNAC 3 cut(s) 179, 320, 385
MalI GATC 7 cut(s) 165, 352, 609, 752, 924, 1047, 1059
MboI GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
MboII GAAGA 4 cut(s) 55, 102, 105, 560
MfeI CAATTG 2 cut(s) 21, 1040
MflI RGATCY 2 cut(s) 350, 1045
MhlI GDGCHC 2 cut(s) 377, 515
MluCI AATT 5 cut(s) 21, 194, 292, 780, 1040
MmeI TCCRAC 1 cut(s) 835
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 1 cut(s) 156
MslI CAYNNNNRTG 3 cut(s) 491, 508, 549
MspI CCGG 2 cut(s) 171, 236
MunI CAATTG 2 cut(s) 21, 1040
MwoI GCNNNNNNNGC 2 cut(s) 159, 636
NdeII GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
NlaIII CATG 4 cut(s) 204, 335, 548, 681
NlaIV GGNNCC 3 cut(s) 432, 531, 1047
NmuCI GTSAC 2 cut(s) 179, 385
OliI CACNNNNGTG 1 cut(s) 508
PagI TCATGA 1 cut(s) 544
PaqCI CACCTGC 1 cut(s) 764
PceI AGGCCT 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 762, 860, 875
PkrI GCNGC 3 cut(s) 176, 266, 269
Ppu21I YACGTR 1 cut(s) 462
PsiI TTATAA 2 cut(s) 417, 558
PspEI GGTNACC 1 cut(s) 179
PspN4I GGNNCC 3 cut(s) 432, 531, 1047
PspPI GGNCC 1 cut(s) 1036
PstI CTGCAG 2 cut(s) 269, 396
PstNI CAGNNNCTG 1 cut(s) 1066
PsuI RGATCY 2 cut(s) 350, 1045
RsaI GTAC 2 cut(s) 143, 485
RsaNI GTAC 2 cut(s) 142, 484
RseI CAYNNNNRTG 3 cut(s) 491, 508, 549
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 3 cut(s) 175, 265, 268
Sau3AI GATC 7 cut(s) 163, 350, 607, 750, 922, 1045, 1057
Sau96I GGNCC 1 cut(s) 1036
SduI GDGCHC 2 cut(s) 377, 515
SfaNI GCATC 3 cut(s) 35, 1018, 1053
SfcI CTRYAG 4 cut(s) 265, 285, 392, 1023
SinI GGWCC 1 cut(s) 1036
SmiMI CAYNNNNRTG 3 cut(s) 491, 508, 549
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 5 cut(s) 21, 194, 292, 780, 1040
SseBI AGGCCT 1 cut(s) 691
SsiI CCGC 3 cut(s) 151, 177, 758
SspI AATATT 1 cut(s) 231
SspMI CTAG 4 cut(s) 50, 473, 687, 1093
StuI AGGCCT 1 cut(s) 691
TaaI ACNGT 3 cut(s) 34, 469, 735
TaiI ACGT 4 cut(s) 246, 318, 464, 512
TaqI TCGA 4 cut(s) 225, 666, 932, 969
TasI AATT 5 cut(s) 21, 194, 292, 780, 1040
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 3 cut(s) 762, 860, 875
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TseFI GTSAC 2 cut(s) 179, 385
TseI GCWGC 3 cut(s) 174, 264, 267
Tsp45I GTSAC 2 cut(s) 179, 385
TspDTI ATGAA 3 cut(s) 111, 561, 588
TspGWI ACGGA 2 cut(s) 273, 440
VpaK11BI GGWCC 1 cut(s) 1036
XapI RAATTY 2 cut(s) 194, 292
XbaI TCTAGA 1 cut(s) 472
XmiI GTMKAC 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 4 cut(s) 50, 473, 687, 1093
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.