Rroxscaffold_5G00375520

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004230
Physical Location & Seq
Forward (+)
56321685 .. 56330338
8654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375520.1

Sequence Viewer

Length: 1107 bp
ATGAAGAGTCTGAGGAAAGAAGAATCCGCAGCTGCATCCATTCTCCTTTATTCTGTGCTGACTTCTCTATTTTTCCTTGCAGTTCAAGCAAGAAGTCATAAACCAGGGTGTGCTTCTTCGTATGGAGATATCGAAAACATCAGCTACCCTTTTCGCCTAAAGGTTGATCCAGTAGGCTGCGGTGACCCTGACTATGAACTATCATGTGTGAACAACAAAACCATCCTCAAAATATTTCCAGGCAAGTACTATGTCAAGAACATTTCTTATGATGAACATATTATCCGTTTGGTTGATGTCAACTTTGCCAATAGAAGCAGCTGCAGCCTTCCATCGGGATCTGTTGTATCGAGGGATGGATATTTTGTGAAGGGCTCCAGAAACATCAGCCAGGCTAATGTAGCTGCTTATAACTACACGGCTGTTCCATGTTTAACTAGAGTTAATGGGACTAATTACTTCTATGCTGTTTATGATGGCTACTACTTAGCTCGTCCTCGTCAACAACCATGCTCGCTTATTTCAGTGGCTATTTCAGATTATCATGAAAATTTTGTTGAGCCCCTATCTTATGAAGCCATCATGAAACAATTGGAGGCAGGGTTTGATCTGGGATGGTCCGTTGAGTGTCGCGACTGCTCTTTAGCTGGAAAATCATGTGGAGTAGATGTAAGTGATCTAAATTTGGGTACTAAGCCACTCCCCTATCAATGTTATGAAGGTACAGAGTACAAAGAACCCACTGAGCTCCAAATAATCTTAATATTTGTGGGAATTGCTTTGGGAGGGTTTGATCTTGGATGGTCCCTCAAGTGTCGTGACTGCTGCTTATCTGGAAATACTTTCTCACAAGTTCATGGGATAAGCCAGTCACAAATGTATGCGAAAAAGAAAAACTCTTTCACATACACGTCACCTACAATTGCTAAGAAGGTCCTTGTTCATAATCAACGACTTACTGATGTGCTCCACTACTCATGCAACTATACTACGAAGTTGTACTCAAATTATACTATGAAAGATGAATTTACTGTTTACACTGTTCATGTAACCTGGTCTTCTACCATAAGTTCACGTACGCCAAAGAAGATCGGTAGTTCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

368

Amino Acids

41.07

Weight (kDa)

8.4

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 99 7.6e-17 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AccII CGCG 1 cut(s) 633
AciI CCGC 2 cut(s) 27, 180
AclWI GGATC 2 cut(s) 161, 346
AcsI RAATTY 3 cut(s) 550, 682, 1025
AfaI GTAC 6 cut(s) 248, 691, 724, 731, 1001, 1078
AfiI CCNNNNNNNGG 1 cut(s) 334
AflIII ACRYGT 1 cut(s) 909
AgsI TTSAA 1 cut(s) 86
AjiI CACGTC 1 cut(s) 912
AjnI CCWGG 4 cut(s) 103, 238, 390, 1052
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 7 cut(s) 32, 144, 321, 404, 491, 647, 748
AluI AGCT 7 cut(s) 32, 144, 321, 404, 491, 647, 748
Alw21I GWGCWC 2 cut(s) 750, 969
AlwI GGATC 2 cut(s) 161, 346
ApeKI GCWGC 8 cut(s) 29, 32, 177, 318, 321, 324, 404, 825
ApoI RAATTY 3 cut(s) 550, 682, 1025
AspS9I GGNCC 3 cut(s) 618, 804, 934
AsuHPI GGTGA 2 cut(s) 194, 906
AvaII GGWCC 3 cut(s) 618, 804, 934
BanII GRGCYC 3 cut(s) 377, 564, 750
BbsI GAAGAC 1 cut(s) 1050
Bbv12I GWGCWC 2 cut(s) 750, 969
BbvI GCAGC 8 cut(s) 19, 41, 164, 308, 330, 336, 391, 812
BccI CCATC 7 cut(s) 230, 340, 350, 470, 587, 609, 795
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 4 cut(s) 105, 240, 392, 1054
BfaI CTAG 1 cut(s) 438
BfmI CTRYAG 1 cut(s) 322
BisI GCNGC 8 cut(s) 30, 33, 178, 319, 322, 325, 405, 826
BlsI GCNGC 8 cut(s) 31, 34, 179, 320, 323, 326, 406, 827
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 4 cut(s) 105, 240, 392, 1054
Bme18I GGWCC 3 cut(s) 618, 804, 934
BmgBI CACGTC 1 cut(s) 912
BmgT120I GGNCC 3 cut(s) 618, 804, 934
BmiI GGNNCC 2 cut(s) 376, 806
BmrFI CCNGG 4 cut(s) 105, 240, 392, 1054
BmsI GCATC 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 1050
BpmI CTGGAG 1 cut(s) 361
BpuEI CTTGAG 1 cut(s) 794
BsaAI YACGTR 1 cut(s) 1076
BsaJI CCNNGG 1 cut(s) 104
BsaXI ACNNNNNCTCC 4 cut(s) 587, 617, 654, 684
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 170, 868
BseBI CCWGG 4 cut(s) 105, 240, 392, 1054
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 5 cut(s) 35, 222, 361, 620, 806
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMII CTCAG 1 cut(s) 735
BseNI ACTGG 2 cut(s) 170, 868
BseXI GCAGC 8 cut(s) 19, 41, 164, 308, 330, 336, 391, 812
Bsh1236I CGCG 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 750, 969
BsiWI CGTACG 1 cut(s) 1076
BslFI GGGAC 2 cut(s) 463, 790
BslI CCNNNNNNNGG 1 cut(s) 334
BsmFI GGGAC 2 cut(s) 463, 790
Bsp1286I GDGCHC 4 cut(s) 377, 564, 750, 969
Bsp143I GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
Bsp68I TCGCGA 1 cut(s) 633
BspACI CCGC 2 cut(s) 27, 180
BspCNI CTCAG 1 cut(s) 736
BspFNI CGCG 1 cut(s) 633
BspHI TCATGA 2 cut(s) 544, 582
BspLI GGNNCC 2 cut(s) 376, 806
BspMAI CTGCAG 1 cut(s) 326
BspPI GGATC 2 cut(s) 161, 346
BsrI ACTGG 2 cut(s) 170, 868
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
Bst2UI CCWGG 4 cut(s) 105, 240, 392, 1054
Bst4CI ACNGT 2 cut(s) 1033, 1042
BstBAI YACGTR 1 cut(s) 1076
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 5 cut(s) 11, 487, 693, 744, 927
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 5 cut(s) 35, 222, 361, 620, 806
BstFNI CGCG 1 cut(s) 633
BstKTI GATC 6 cut(s) 169, 341, 610, 679, 796, 1092
BstMBI GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
BstMWI GCNNNNNNNGC 3 cut(s) 86, 324, 401
BstNI CCWGG 4 cut(s) 105, 240, 392, 1054
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 4 cut(s) 103, 238, 390, 1052
BstSFI CTRYAG 1 cut(s) 322
BstUI CGCG 1 cut(s) 633
BstV1I GCAGC 8 cut(s) 19, 41, 164, 308, 330, 336, 391, 812
BstV2I GAAGAC 1 cut(s) 1050
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 5 cut(s) 35, 222, 361, 620, 806
BtsIMutI CAGTG 3 cut(s) 531, 741, 1038
BtuMI TCGCGA 1 cut(s) 633
Cac8I GCNNGC 1 cut(s) 515
CciI TCATGA 2 cut(s) 544, 582
Cfr13I GGNCC 3 cut(s) 618, 804, 934
CsiI ACCWGGT 1 cut(s) 1052
Csp6I GTAC 6 cut(s) 247, 690, 723, 730, 1000, 1077
CviAII CATG 9 cut(s) 204, 429, 510, 545, 583, 657, 857, 978, 1046
CviQI GTAC 6 cut(s) 247, 690, 723, 730, 1000, 1077
DdeI CTNAG 5 cut(s) 11, 487, 693, 744, 927
DpnI GATC 6 cut(s) 168, 340, 609, 678, 795, 1091
DpnII GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
Ecl136II GAGCTC 1 cut(s) 748
Eco24I GRGCYC 3 cut(s) 377, 564, 750
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 3 cut(s) 618, 804, 934
Eco53kI GAGCTC 1 cut(s) 748
Eco91I GGTNACC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 748
EcoO109I RGGNCCY 1 cut(s) 934
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 4 cut(s) 103, 238, 390, 1052
EcoRV GATATC 1 cut(s) 130
EcoT38I GRGCYC 3 cut(s) 377, 564, 750
FaeI CATG 9 cut(s) 207, 432, 513, 548, 586, 660, 860, 981, 1049
FaqI GGGAC 2 cut(s) 463, 790
FatI CATG 9 cut(s) 203, 428, 509, 544, 582, 656, 856, 977, 1045
Fnu4HI GCNGC 8 cut(s) 30, 33, 178, 319, 322, 325, 405, 826
FokI GGATG 5 cut(s) 22, 209, 368, 627, 813
FriOI GRGCYC 3 cut(s) 377, 564, 750
Fsp4HI GCNGC 8 cut(s) 30, 33, 178, 319, 322, 325, 405, 826
FspBI CTAG 1 cut(s) 438
GluI GCNGC 8 cut(s) 30, 33, 178, 319, 322, 325, 405, 826
GsuI CTGGAG 1 cut(s) 361
Hin1II CATG 9 cut(s) 207, 432, 513, 548, 586, 660, 860, 981, 1049
HincII GTYRAC 2 cut(s) 301, 503
HindII GTYRAC 2 cut(s) 301, 503
HinfI GANTC 2 cut(s) 7, 23
HphI GGTGA 2 cut(s) 194, 906
Hpy166II GTNNAC 5 cut(s) 211, 301, 503, 1036, 1073
Hpy188I TCNGA 2 cut(s) 12, 538
Hpy188III TCNNGA 8 cut(s) 256, 336, 378, 545, 583, 632, 818, 834
Hpy8I GTNNAC 5 cut(s) 211, 301, 503, 1036, 1073
HpyAV CCTTC 4 cut(s) 338, 364, 713, 925
HpyCH4III ACNGT 2 cut(s) 1033, 1042
HpyCH4IV ACGT 2 cut(s) 911, 1075
HpyCH4V TGCA 4 cut(s) 35, 80, 324, 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 324, 401
HpyF3I CTNAG 5 cut(s) 11, 487, 693, 744, 927
HpySE526I ACGT 2 cut(s) 911, 1075
Hsp92II CATG 9 cut(s) 207, 432, 513, 548, 586, 660, 860, 981, 1049
Kzo9I GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
LmnI GCTCC 3 cut(s) 380, 753, 972
Lsp1109I GCAGC 8 cut(s) 19, 41, 164, 308, 330, 336, 391, 812
LweI GCATC 1 cut(s) 44
MabI ACCWGGT 1 cut(s) 1052
MaeI CTAG 1 cut(s) 438
MaeII ACGT 2 cut(s) 911, 1075
MaeIII GTNAC 5 cut(s) 182, 818, 870, 912, 1048
MalI GATC 6 cut(s) 168, 340, 609, 678, 795, 1091
MboI GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
MboII GAAGA 5 cut(s) 16, 32, 108, 1050, 1099
MfeI CAATTG 2 cut(s) 590, 921
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 4 cut(s) 377, 564, 750, 969
MluCI AATT 8 cut(s) 454, 550, 590, 682, 774, 921, 1006, 1025
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 7 cut(s) 6, 236, 345, 507, 589, 779, 818
MseI TTAA 3 cut(s) 434, 444, 761
MspA1I CMGCKG 2 cut(s) 32, 321
MspR9I CCNGG 4 cut(s) 105, 240, 392, 1054
MunI CAATTG 2 cut(s) 590, 921
MvaI CCWGG 4 cut(s) 105, 240, 392, 1054
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 3 cut(s) 86, 324, 401
NdeII GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
NlaIII CATG 9 cut(s) 207, 432, 513, 548, 586, 660, 860, 981, 1049
NlaIV GGNNCC 2 cut(s) 376, 806
NmuCI GTSAC 4 cut(s) 182, 818, 870, 912
NruI TCGCGA 1 cut(s) 633
PagI TCATGA 2 cut(s) 544, 582
PfeI GAWTC 1 cut(s) 23
Pfl23II CGTACG 1 cut(s) 1076
PkrI GCNGC 8 cut(s) 31, 34, 179, 320, 323, 326, 406, 827
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 1076
PpuMI RGGWCCY 1 cut(s) 934
PsiI TTATAA 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 934
Psp6I CCWGG 4 cut(s) 103, 238, 390, 1052
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 4 cut(s) 103, 238, 390, 1052
PspLI CGTACG 1 cut(s) 1076
PspN4I GGNNCC 2 cut(s) 376, 806
PspPI GGNCC 3 cut(s) 618, 804, 934
PspPPI RGGWCCY 1 cut(s) 934
PstI CTGCAG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 338
PvuII CAGCTG 2 cut(s) 32, 321
RruI TCGCGA 1 cut(s) 633
RsaI GTAC 6 cut(s) 248, 691, 724, 731, 1001, 1078
RsaNI GTAC 6 cut(s) 247, 690, 723, 730, 1000, 1077
SacI GAGCTC 1 cut(s) 750
SaqAI TTAA 3 cut(s) 434, 444, 761
SatI GCNGC 8 cut(s) 30, 33, 178, 319, 322, 325, 405, 826
Sau3AI GATC 6 cut(s) 166, 338, 607, 676, 793, 1089
Sau96I GGNCC 3 cut(s) 618, 804, 934
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 4 cut(s) 105, 240, 392, 1054
SduI GDGCHC 4 cut(s) 377, 564, 750, 969
SexAI ACCWGGT 1 cut(s) 1052
SfaNI GCATC 1 cut(s) 44
SfcI CTRYAG 1 cut(s) 322
SinI GGWCC 3 cut(s) 618, 804, 934
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
Sse9I AATT 8 cut(s) 454, 550, 590, 682, 774, 921, 1006, 1025
SsiI CCGC 2 cut(s) 27, 180
SspI AATATT 2 cut(s) 234, 765
SspMI CTAG 1 cut(s) 438
SstI GAGCTC 1 cut(s) 750
StyD4I CCNGG 4 cut(s) 103, 238, 390, 1052
TaaI ACNGT 2 cut(s) 1033, 1042
TaiI ACGT 2 cut(s) 914, 1078
TaqI TCGA 2 cut(s) 132, 350
TasI AATT 8 cut(s) 454, 550, 590, 682, 774, 921, 1006, 1025
TatI WGTACW 3 cut(s) 246, 729, 999
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 3 cut(s) 434, 444, 761
Tru9I TTAA 3 cut(s) 434, 444, 761
TscAI CASTG 3 cut(s) 531, 748, 1045
TseFI GTSAC 4 cut(s) 182, 818, 870, 912
TseI GCWGC 8 cut(s) 29, 32, 177, 318, 321, 324, 404, 825
Tsp45I GTSAC 4 cut(s) 182, 818, 870, 912
TspGWI ACGGA 2 cut(s) 275, 610
TspRI CASTG 3 cut(s) 531, 748, 1045
VpaK11BI GGWCC 3 cut(s) 618, 804, 934
XapI RAATTY 3 cut(s) 550, 682, 1025
XspI CTAG 1 cut(s) 438
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.