FvH4_4g26500

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004230
Physical Location & Seq
Forward (+)
27880008 .. 27881624
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26500.t2

Sequence Viewer

Length: 942 bp
ATGAGGAAACGCTCTACATCAGTCGCATCAATCATTCTTCTCCCTGTCCTTATCTTCTCCCTTTTTCTTATCCATGTTCAAGGGAAGTTGAATGAAGCTGGCTGTTCTTCTTCCTGTGGAGATATTGACAACATCAAGTACCCTTTTCGCGTTAGAGGCGATCCATCCGGCTGCGGTGACCCCGACTATGAATTCTCATGTGTCAACAACAAAACCATCCTCGAAATATTTCCGGGCAAATACTATGTCAAGAGCATTTCATACACTGATAATCTACTCCATTTAGTTGACGTCAACTTCGCAAACGGAAGCTGCAGCCTCCCATCTGGTTCTCTTAAAAATAGGGATGGTGTTGCCCAAGACTTTCGTTACAAGGGTGAAGTCGACTTCGCAACTGAATCTCTTATCCGATTTGTCAAGTGCTCTAGTAATGTCACACTGCAGTTTAATCAGACACTGCATTATAACTACACGGAGGTTCCTTGTTTGTCAAGAAATGGAACTTACATGTATGCTGTTGTAGATGGAAGGTACGAGGCTAACAATTATCCACGGACGTGCTCGCTTGTTTCGCTGGCTCCGGTGAATTATCATGATGAACTTTATAAGAAGATCCCTACTTATGAAGCCGTAGCGAAAGCGTTGCAGGCCGGGTTTGATATCGGATGGTCTGTTGAGTGCAGGGATTGTTCTTTGGCTGGTAAGTATTGTGTATACGGTCCCAAAGCATCGGACACTAGGCCTATCATATTCGAATGTCAAAAGGAGTACCATGAAATCACAAGGTTTGAAGGAAATTTGATCATTGCGGGAATCGTTGTGGGAGGTGTAATTGGTCTAGTTATTATAGTGGCTGTGTTGGTGTGTGTTATCTGCAAATGTAAGAAACGAAAACAGCAGCCGAAGAAGAATCTGCAGAACCAGCAATCATCGACACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

314

Amino Acids

34.87

Weight (kDa)

8.53

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 97 9.1e-18 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 465, 606
AatII GACGTC 1 cut(s) 294
AccI GTMKAC 2 cut(s) 384, 714
AccII CGCG 1 cut(s) 150
AciI CCGC 2 cut(s) 174, 809
AclWI GGATC 2 cut(s) 155, 607
AcsI RAATTY 2 cut(s) 191, 796
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 3 cut(s) 140, 533, 770
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 3 cut(s) 80, 91, 791
AjiI CACGTC 1 cut(s) 558
AleI CACNNNNGTG 1 cut(s) 556
AluBI AGCT 2 cut(s) 98, 312
AluI AGCT 2 cut(s) 98, 312
Alw21I GWGCWC 2 cut(s) 425, 563
AlwI GGATC 2 cut(s) 155, 607
AlwNI CAGNNNCTG 1 cut(s) 457
AoxI GGCC 2 cut(s) 648, 740
ApeKI GCWGC 4 cut(s) 171, 312, 315, 898
ApoI RAATTY 2 cut(s) 191, 796
ArsI GACNNNNNNTTYG 4 cut(s) 231, 263, 281, 313
Asp700I GAANNNNTTC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 719
AsuC2I CCSGG 2 cut(s) 234, 652
AsuHPI GGTGA 3 cut(s) 188, 389, 595
AsuII TTCGAA 1 cut(s) 753
AvaII GGWCC 1 cut(s) 719
Bbv12I GWGCWC 2 cut(s) 425, 563
BbvI GCAGC 4 cut(s) 158, 299, 327, 910
BccI CCATC 6 cut(s) 172, 224, 331, 341, 518, 660
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 625, 659
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 2 cut(s) 234, 652
BfaI CTAG 3 cut(s) 426, 738, 839
BfmI CTRYAG 3 cut(s) 313, 440, 914
BisI GCNGC 4 cut(s) 172, 313, 316, 899
BlsI GCNGC 4 cut(s) 173, 314, 317, 900
Bme1390I CCNGG 2 cut(s) 234, 652
Bme18I GGWCC 1 cut(s) 719
BmgBI CACGTC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 3 cut(s) 480, 579, 721
BmrFI CCNGG 2 cut(s) 234, 652
BmsI GCATC 2 cut(s) 35, 737
Bpu14I TTCGAA 1 cut(s) 753
BpuMI CCSGG 2 cut(s) 234, 652
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 551
BsaWI WCCGGW 1 cut(s) 580
Bse3DI GCAATG 1 cut(s) 804
BseDI CCNNGG 1 cut(s) 551
BseGI GGATG 4 cut(s) 164, 216, 352, 671
BseMI GCAATG 1 cut(s) 804
BseXI GCAGC 4 cut(s) 158, 299, 327, 910
BsgI GTGCAG 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 650, 742
BsiHKAI GWGCWC 2 cut(s) 425, 563
BsiSI CCGG 4 cut(s) 168, 233, 581, 651
BslFI GGGAC 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 705
BsnI GGCC 2 cut(s) 650, 742
Bsp119I TTCGAA 1 cut(s) 753
Bsp1286I GDGCHC 2 cut(s) 425, 563
Bsp143I GATC 3 cut(s) 160, 612, 801
BspACI CCGC 2 cut(s) 174, 809
BspANI GGCC 2 cut(s) 650, 742
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 3 cut(s) 480, 579, 721
BspMAI CTGCAG 3 cut(s) 317, 444, 918
BspPI GGATC 2 cut(s) 155, 607
BspT104I TTCGAA 1 cut(s) 753
BsrDI GCAATG 1 cut(s) 804
BssECI CCNNGG 1 cut(s) 551
BssMI GATC 3 cut(s) 160, 612, 801
BssNAI GTATAC 1 cut(s) 715
BssNI GRCGYC 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 715
Bst4CI ACNGT 1 cut(s) 719
BstACI GRCGYC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 753
BstC8I GCNNGC 4 cut(s) 100, 563, 576, 648
BstDSI CCRYGG 1 cut(s) 551
BstEII GGTNACC 1 cut(s) 176
BstF5I GGATG 4 cut(s) 164, 216, 352, 671
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 3 cut(s) 163, 615, 804
BstMBI GATC 3 cut(s) 160, 612, 801
BstMWI GCNNNNNNNGC 4 cut(s) 156, 571, 647, 922
BstNSI RCATGY 1 cut(s) 511
BstPI GGTNACC 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 232, 650
BstSFI CTRYAG 3 cut(s) 313, 440, 914
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 4 cut(s) 158, 299, 327, 910
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
BstZ17I GTATAC 1 cut(s) 715
BsuRI GGCC 2 cut(s) 650, 742
BtgI CCRYGG 1 cut(s) 551
BtrI CACGTC 1 cut(s) 558
BtsCI GGATG 4 cut(s) 164, 216, 352, 671
BtsI GCAGTG 2 cut(s) 437, 455
BtsIMutI CAGTG 3 cut(s) 264, 437, 455
Cac8I GCNNGC 4 cut(s) 100, 563, 576, 648
CaiI CAGNNNCTG 1 cut(s) 457
CciI TCATGA 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 719
Csp6I GTAC 3 cut(s) 139, 532, 769
CviAII CATG 5 cut(s) 74, 198, 508, 593, 773
CviQI GTAC 3 cut(s) 139, 532, 769
DpnI GATC 3 cut(s) 162, 614, 803
DpnII GATC 3 cut(s) 160, 612, 801
Eco147I AGGCCT 1 cut(s) 742
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 719
Eco91I GGTNACC 1 cut(s) 176
EcoO65I GGTNACC 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 191
EcoRV GATATC 1 cut(s) 661
FaeI CATG 5 cut(s) 77, 201, 511, 596, 776
FaqI GGGAC 1 cut(s) 705
FatI CATG 5 cut(s) 73, 197, 507, 592, 772
FauI CCCGC 1 cut(s) 802
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 2 cut(s) 384, 714
Fnu4HI GCNGC 4 cut(s) 172, 313, 316, 899
FokI GGATG 4 cut(s) 151, 203, 359, 678
Fsp4HI GCNGC 4 cut(s) 172, 313, 316, 899
FspBI CTAG 3 cut(s) 426, 738, 839
GluI GCNGC 4 cut(s) 172, 313, 316, 899
HaeIII GGCC 2 cut(s) 650, 742
HapII CCGG 4 cut(s) 168, 233, 581, 651
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 5 cut(s) 77, 201, 511, 596, 776
HincII GTYRAC 4 cut(s) 205, 289, 295, 385
HindII GTYRAC 4 cut(s) 205, 289, 295, 385
HinfI GANTC 3 cut(s) 398, 813, 910
HpaII CCGG 4 cut(s) 168, 233, 581, 651
HphI GGTGA 3 cut(s) 188, 389, 595
Hpy166II GTNNAC 5 cut(s) 205, 289, 295, 385, 715
Hpy188I TCNGA 5 cut(s) 410, 453, 665, 733, 941
Hpy188III TCNNGA 3 cut(s) 250, 492, 593
Hpy8I GTNNAC 5 cut(s) 205, 289, 295, 385, 715
HpyAV CCTTC 2 cut(s) 522, 785
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 2 cut(s) 291, 557
HpyCH4V TGCA 7 cut(s) 315, 442, 460, 646, 681, 876, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 571, 647, 922
HpySE526I ACGT 2 cut(s) 291, 557
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 5 cut(s) 77, 201, 511, 596, 776
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 3 cut(s) 160, 612, 801
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 4 cut(s) 158, 299, 327, 910
LweI GCATC 2 cut(s) 35, 737
MaeI CTAG 3 cut(s) 426, 738, 839
MaeII ACGT 2 cut(s) 291, 557
MaeIII GTNAC 3 cut(s) 176, 368, 433
MalI GATC 3 cut(s) 162, 614, 803
MboI GATC 3 cut(s) 160, 612, 801
MboII GAAGA 7 cut(s) 29, 46, 99, 102, 622, 916, 919
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 2 cut(s) 425, 563
MluCI AATT 5 cut(s) 191, 544, 586, 796, 831
MnlI CCTC 6 cut(s) 149, 230, 329, 469, 529, 818
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 2 cut(s) 336, 447
MslI CAYNNNNRTG 1 cut(s) 556
MspI CCGG 4 cut(s) 168, 233, 581, 651
MspR9I CCNGG 2 cut(s) 234, 652
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 4 cut(s) 156, 571, 647, 922
NciI CCSGG 2 cut(s) 234, 652
NdeII GATC 3 cut(s) 160, 612, 801
NlaIII CATG 5 cut(s) 77, 201, 511, 596, 776
NlaIV GGNNCC 3 cut(s) 480, 579, 721
NmuCI GTSAC 2 cut(s) 176, 433
NspI RCATGY 1 cut(s) 511
NspV TTCGAA 1 cut(s) 753
OliI CACNNNNGTG 1 cut(s) 556
PagI TCATGA 1 cut(s) 592
PceI AGGCCT 1 cut(s) 742
PciI ACATGT 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 3 cut(s) 398, 813, 910
PkrI GCNGC 4 cut(s) 173, 314, 317, 900
PscI ACATGT 1 cut(s) 507
PsiI TTATAA 2 cut(s) 465, 606
PspEI GGTNACC 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 480, 579, 721
PspPI GGNCC 1 cut(s) 719
PstI CTGCAG 3 cut(s) 317, 444, 918
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 612
RsaI GTAC 3 cut(s) 140, 533, 770
RsaNI GTAC 3 cut(s) 139, 532, 769
RseI CAYNNNNRTG 1 cut(s) 556
SalI GTCGAC 1 cut(s) 383
SaqAI TTAA 2 cut(s) 336, 447
SatI GCNGC 4 cut(s) 172, 313, 316, 899
Sau3AI GATC 3 cut(s) 160, 612, 801
Sau96I GGNCC 1 cut(s) 719
ScrFI CCNGG 2 cut(s) 234, 652
SduI GDGCHC 2 cut(s) 425, 563
SetI ASST 8 cut(s) 100, 294, 314, 480, 533, 560, 788, 829
SfaNI GCATC 2 cut(s) 35, 737
SfcI CTRYAG 3 cut(s) 313, 440, 914
SfuI TTCGAA 1 cut(s) 753
SinI GGWCC 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 556
Sse9I AATT 5 cut(s) 191, 544, 586, 796, 831
SseBI AGGCCT 1 cut(s) 742
SsiI CCGC 2 cut(s) 174, 809
SspI AATATT 1 cut(s) 228
SspMI CTAG 3 cut(s) 426, 738, 839
StuI AGGCCT 1 cut(s) 742
StyD4I CCNGG 2 cut(s) 232, 650
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 2 cut(s) 294, 560
TaqI TCGA 4 cut(s) 222, 384, 753, 932
TasI AATT 5 cut(s) 191, 544, 586, 796, 831
TfiI GAWTC 3 cut(s) 398, 813, 910
Tru1I TTAA 2 cut(s) 336, 447
Tru9I TTAA 2 cut(s) 336, 447
TscAI CASTG 3 cut(s) 271, 444, 462
TseFI GTSAC 2 cut(s) 176, 433
TseI GCWGC 4 cut(s) 171, 312, 315, 898
Tsp45I GTSAC 2 cut(s) 176, 433
TspDTI ATGAA 6 cut(s) 108, 204, 249, 612, 639, 789
TspGWI ACGGA 3 cut(s) 321, 488, 568
TspRI CASTG 3 cut(s) 271, 444, 462
VpaK11BI GGWCC 1 cut(s) 719
XapI RAATTY 2 cut(s) 191, 796
XceI RCATGY 1 cut(s) 511
XmiI GTMKAC 2 cut(s) 384, 714
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 3 cut(s) 426, 738, 839
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.