Rh4BG337000

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004230
Physical Location & Seq
Forward (+)
50021913 .. 50023497
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG337000.1

Sequence Viewer

Length: 822 bp
ATGAGGAAAGGCTCAACATCAATTGCATCAACCGTTCTTTTTCCTGTCCTAGTTTTTCTACTCTTCCTTGTTCAAGTTCAAGCAAGAAACTTGAATGAAGCAGCTGATTGTTCTTCTTCCTGTGGAGATATCCACAACATCAAGTACCCTTTCCGCCTTAAAGGCGATCCATCCGGCTGCGGTGACCCCGACTTTGAATTCTCATGTGTCAACAACAAAACCATCCTCGAAATATTTCCGGGTAAATACTACGTCAAAAACATTTCATACACTGACAATCTACTCCGTTTAGTTGATGTCAACTTCGCAAACGGAAGCTGCAGCCTCCCATCTGGTTCTATAGAAAATTCAGATGGTTTCGTCCAAGACATTCGTTACAAGGGTCATGTCAACTACACAACTGGATCTCGTATCCGATTTGTCAAGTGCTCAAGAAATGTCACCCTGCAGAATAATGAGAAGTATTATTATAACTACACGGAGGTTCCTTGTTTGACAAGAAATGGAACTTACACGTATGCTGTTCTAGATGGAATGTACACGGCTATGGATTATCCACCGACGTGCTCACTTGTTTCGCTGGCTCCTGTGGATTATCATGAAGATGTTTATAAGTCCCCTACTTATGAAGCCATAGTGAAAGTGTTGCAGGCAGGGTTTGATCTCGGTTGGTCTGTTGAGTGCAGGGATTGCTCTTTGGCTGGTAAGCATTGCGTAATCGATGACAAAGCATGGGACACTAGGCCTATCATATTCCAATGTGCAAAAGGTATGTGCCTAAGTTTATGCTCTTCACCCTTATGTGTTTCGAACTGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

273

Amino Acids

30.48

Weight (kDa)

6.08

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 99 9.6e-20 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 471, 612
AciI CCGC 2 cut(s) 154, 180
AclWI GGATC 2 cut(s) 161, 412
AcsI RAATTY 2 cut(s) 197, 346
AfaI GTAC 2 cut(s) 146, 539
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 4 cut(s) 74, 80, 94, 197
AjiI CACGTC 1 cut(s) 564
AleI CACNNNNGTG 1 cut(s) 562
AluBI AGCT 2 cut(s) 104, 318
AluI AGCT 2 cut(s) 104, 318
Alw21I GWGCWC 2 cut(s) 431, 569
AlwI GGATC 2 cut(s) 161, 412
AoxI GGCC 1 cut(s) 743
ApeKI GCWGC 4 cut(s) 101, 177, 318, 321
ApoI RAATTY 2 cut(s) 197, 346
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
Asp700I GAANNNNTTC 1 cut(s) 234
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 194, 433, 786
AsuII TTCGAA 1 cut(s) 809
Bbv12I GWGCWC 2 cut(s) 431, 569
BbvI GCAGC 4 cut(s) 113, 164, 305, 333
BccI CCATC 5 cut(s) 178, 230, 337, 347, 524
BceAI ACGGC 1 cut(s) 558
BciVI GTATCC 1 cut(s) 422
BcnI CCSGG 1 cut(s) 240
BfaI CTAG 3 cut(s) 50, 527, 741
BfmI CTRYAG 3 cut(s) 319, 339, 446
BfuI GTATCC 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 162
BisI GCNGC 4 cut(s) 102, 178, 319, 322
BlsI GCNGC 4 cut(s) 103, 179, 320, 323
Bme1390I CCNGG 1 cut(s) 240
BmgBI CACGTC 1 cut(s) 564
BmiI GGNNCC 2 cut(s) 486, 585
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 516
Bse1I ACTGG 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 709
BseCI ATCGAT 1 cut(s) 720
BseGI GGATG 2 cut(s) 170, 222
BseMI GCAATG 1 cut(s) 709
BseNI ACTGG 1 cut(s) 406
BseXI GCAGC 4 cut(s) 113, 164, 305, 333
BsgI GTGCAG 1 cut(s) 703
BshFI GGCC 1 cut(s) 745
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 2 cut(s) 431, 569
BsiSI CCGG 2 cut(s) 174, 239
BslFI GGGAC 2 cut(s) 601, 749
BsmFI GGGAC 2 cut(s) 601, 749
BsnI GGCC 1 cut(s) 745
Bsp119I TTCGAA 1 cut(s) 809
Bsp1286I GDGCHC 2 cut(s) 431, 569
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 3 cut(s) 166, 404, 661
BspACI CCGC 2 cut(s) 154, 180
BspANI GGCC 1 cut(s) 745
BspDI ATCGAT 1 cut(s) 720
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 2 cut(s) 486, 585
BspMAI CTGCAG 2 cut(s) 323, 450
BspPI GGATC 2 cut(s) 161, 412
BspQI GCTCTTC 1 cut(s) 796
BspT104I TTCGAA 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 709
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 1 cut(s) 406
BssMI GATC 3 cut(s) 166, 404, 661
Bst4CI ACNGT 2 cut(s) 34, 815
Bst6I CTCTTC 2 cut(s) 68, 796
BstAPI GCANNNNNTGC 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 516
BstBI TTCGAA 1 cut(s) 809
BstC8I GCNNGC 2 cut(s) 582, 651
BstDEI CTNAG 1 cut(s) 779
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 2 cut(s) 170, 222
BstKTI GATC 3 cut(s) 169, 407, 664
BstMBI GATC 3 cut(s) 166, 404, 661
BstMWI GCNNNNNNNGC 2 cut(s) 162, 690
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 3 cut(s) 319, 339, 446
BstV1I GCAGC 4 cut(s) 113, 164, 305, 333
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
Bsu15I ATCGAT 1 cut(s) 720
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 745
BsuTUI ATCGAT 1 cut(s) 720
BtrI CACGTC 1 cut(s) 564
BtsCI GGATG 2 cut(s) 170, 222
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 2 cut(s) 582, 651
CciI TCATGA 1 cut(s) 598
ClaI ATCGAT 1 cut(s) 720
Csp6I GTAC 2 cut(s) 145, 538
CviAII CATG 4 cut(s) 204, 386, 599, 732
CviQI GTAC 2 cut(s) 145, 538
DdeI CTNAG 1 cut(s) 779
DpnI GATC 3 cut(s) 168, 406, 663
DpnII GATC 3 cut(s) 166, 404, 661
Eam1104I CTCTTC 2 cut(s) 68, 796
EarI CTCTTC 2 cut(s) 68, 796
EciI GGCGGA 1 cut(s) 143
Eco147I AGGCCT 1 cut(s) 745
Eco32I GATATC 1 cut(s) 130
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 197
EcoRV GATATC 1 cut(s) 130
FaeI CATG 4 cut(s) 207, 389, 602, 735
FaqI GGGAC 2 cut(s) 601, 749
FatI CATG 4 cut(s) 203, 385, 598, 731
Fnu4HI GCNGC 4 cut(s) 102, 178, 319, 322
FokI GGATG 2 cut(s) 157, 209
Fsp4HI GCNGC 4 cut(s) 102, 178, 319, 322
FspBI CTAG 3 cut(s) 50, 527, 741
GluI GCNGC 4 cut(s) 102, 178, 319, 322
HaeIII GGCC 1 cut(s) 745
HapII CCGG 2 cut(s) 174, 239
Hin1II CATG 4 cut(s) 207, 389, 602, 735
HincII GTYRAC 3 cut(s) 211, 301, 391
HindII GTYRAC 3 cut(s) 211, 301, 391
HpaII CCGG 2 cut(s) 174, 239
HphI GGTGA 3 cut(s) 194, 433, 786
Hpy166II GTNNAC 4 cut(s) 211, 301, 391, 540
Hpy188I TCNGA 2 cut(s) 352, 416
Hpy188III TCNNGA 3 cut(s) 432, 527, 599
Hpy8I GTNNAC 4 cut(s) 211, 301, 391, 540
Hpy99I CGWCG 1 cut(s) 565
HpyCH4III ACNGT 2 cut(s) 34, 815
HpyCH4IV ACGT 3 cut(s) 252, 515, 563
HpyCH4V TGCA 6 cut(s) 26, 321, 448, 649, 684, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 690
HpyF3I CTNAG 1 cut(s) 779
HpySE526I ACGT 3 cut(s) 252, 515, 563
Hsp92II CATG 4 cut(s) 207, 389, 602, 735
Kzo9I GATC 3 cut(s) 166, 404, 661
LguI GCTCTTC 1 cut(s) 796
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 4 cut(s) 113, 164, 305, 333
LweI GCATC 1 cut(s) 35
MaeI CTAG 3 cut(s) 50, 527, 741
MaeII ACGT 3 cut(s) 252, 515, 563
MaeIII GTNAC 3 cut(s) 182, 374, 439
MalI GATC 3 cut(s) 168, 406, 663
MboI GATC 3 cut(s) 166, 404, 661
MboII GAAGA 5 cut(s) 55, 105, 108, 614, 783
MfeI CAATTG 1 cut(s) 21
MflI RGATCY 1 cut(s) 404
MhlI GDGCHC 2 cut(s) 431, 569
MluCI AATT 3 cut(s) 21, 197, 346
MnlI CCTC 3 cut(s) 236, 335, 475
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 4 cut(s) 545, 562, 603, 799
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 2 cut(s) 174, 239
MspR9I CCNGG 1 cut(s) 240
MunI CAATTG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 162, 690
NciI CCSGG 1 cut(s) 240
NdeII GATC 3 cut(s) 166, 404, 661
NlaIII CATG 4 cut(s) 207, 389, 602, 735
NlaIV GGNNCC 2 cut(s) 486, 585
NmuCI GTSAC 2 cut(s) 182, 439
NspV TTCGAA 1 cut(s) 809
OliI CACNNNNGTG 1 cut(s) 562
PagI TCATGA 1 cut(s) 598
PceI AGGCCT 1 cut(s) 745
PciSI GCTCTTC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 234
PkrI GCNGC 4 cut(s) 103, 179, 320, 323
Ppu21I YACGTR 1 cut(s) 516
PsiI TTATAA 2 cut(s) 471, 612
PspEI GGTNACC 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 486, 585
PstI CTGCAG 2 cut(s) 323, 450
PsuI RGATCY 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 146, 539
RsaNI GTAC 2 cut(s) 145, 538
RseI CAYNNNNRTG 4 cut(s) 545, 562, 603, 799
SapI GCTCTTC 1 cut(s) 796
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 4 cut(s) 102, 178, 319, 322
Sau3AI GATC 3 cut(s) 166, 404, 661
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 2 cut(s) 431, 569
SetI ASST 7 cut(s) 106, 255, 320, 486, 518, 566, 772
SfaNI GCATC 1 cut(s) 35
SfcI CTRYAG 3 cut(s) 319, 339, 446
SfuI TTCGAA 1 cut(s) 809
SmiMI CAYNNNNRTG 4 cut(s) 545, 562, 603, 799
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 3 cut(s) 21, 197, 346
SseBI AGGCCT 1 cut(s) 745
SsiI CCGC 2 cut(s) 154, 180
SspI AATATT 1 cut(s) 234
SspMI CTAG 3 cut(s) 50, 527, 741
StuI AGGCCT 1 cut(s) 745
StyD4I CCNGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 34, 815
TaiI ACGT 3 cut(s) 255, 518, 566
TaqI TCGA 3 cut(s) 228, 720, 809
TasI AATT 3 cut(s) 21, 197, 346
TatI WGTACW 1 cut(s) 537
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 2 cut(s) 182, 439
TseI GCWGC 4 cut(s) 101, 177, 318, 321
Tsp45I GTSAC 2 cut(s) 182, 439
TspDTI ATGAA 4 cut(s) 111, 255, 615, 642
TspGWI ACGGA 3 cut(s) 275, 327, 494
TspRI CASTG 1 cut(s) 277
XapI RAATTY 2 cut(s) 197, 346
XbaI TCTAGA 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 3 cut(s) 50, 527, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.