Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004230

Physical Location & Seq

Genomic Coordinates Forward (+)

2374346 .. 2375389

Gene Structure 1044 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1033800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 870 bp

ATGAGGACGGTCCAATATACATCTTCTCTTTTCTGTTCTCTGCTGATTCTGCTATTTTTCGTTCAAATTCAAGCACAAACCAACTGTTGGTCTTCCTGCGGAGAAATAAAAAACATCAGCTACCCTTTTCGCCTCAAGGGCGATCCATCCGGCTGCGGCGACTCCGATTACGAGCTCTCTTGTGTCGACAACAAACCCATCCTAGAGATTTTCCCCGGGAAATACTACGTGCGCAACATTTCCTACTATGATCACACACTCCGTTTGGTCGACGTCAACTTCGCCAACGGAACCTGCGGCCTTCCGTCTGGTCCTGTCGAAACAACGAACGGGTTTGTCCGGGATGCTCGATTCATGGGATATGGGTGGAACCCAGGAAGTCGTTTCCGGTTCGTCAAGTGCTCAACAAACATCAGCAGCATGCCGGAAGCTGCTAATCACACAGTGGTTCCTTGCCTGACAAGAAACGGGACTTACGTCTATGCTGTTTATGATGGCGATTACTCGTATTATGAGCCTCAAAAATCATGCTCTGTCATTTCGTTGGCTCCTGTCGATCTTCGTAAAGATGTTACGAAGTTCAATTCTTATGAGGCCGTCATGGAACTATTGAAAGCTGGCTTTGAAGTTGAATGGTCAGTTACGTGCAGAGATTGCTCTTTGGCTAGTAAACAGTGCGTAGTAAAATCAATGGATAAGCCATTCACCTATATCTGCAAAAAAGAGTACAAAGAACTGACAAGAGCTGAAGGAAACTGGATAATTGCTGCAATCGTTGTGGGAGGTAATATTACTGTCGCATTTGTACAAACATTGACAAACTTGTTATTTTCTTTCAATTTTTCGATCTCTTTCCCGAGTTGCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind WAK_assoc

Protein Analysis

290

Amino Acids

32.44

Weight (kDa)

7.37

Isoelectric Point (pI)

32.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	29 - 91	2.9e-18	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	164 - 240	8.7e-06	Wall-associated receptor kinase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004230)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g26480 FvH4_4g26500 FvH4_4g26500
malus_domestica	MD13G1033700.v1.1 MD13G1033800.v1.1 MD16G1035600.v1.1
prunus_persica	Prupe.1G319000_v2.0.a1 Prupe.1G319100_v2.0.a1
pyrus_communis	pycom13g02910 pycom13g02920 pycom16g03050
rosa_chinensis	RchiOBHm_Chr4g0434121 RchiOBHm_Chr4g0434131
rosa_laevigata	RLG00000006697 RLG00000006698
rosa_multiflora	Rmu_sc0000352.1_g000036
rosa_roxburghii	Rroxscaffold_5G00375520 Rroxscaffold_5G00375530
rosa_rugosa	Rorug04G0274500 Rorug04G0274500 Rorug04G0274500 Rorug04G0274500 Rorug04G0274500
rosa_samantha	Rh4AG329400 Rh4AG329500 Rh4BG336900 Rh4BG337000 Rh4CG352400 Rh4CG352500 Rh4DG333000 Rh4DG333100
rosa_wichuraiana	Rw4G028560 Rw4G028570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	276
Acc16I	TGCGCA	1 cut(s)	233
Acc36I	ACCTGC	1 cut(s)	302
AccB7I	CCANNNNNTGG	1 cut(s)	87
AccI	GTMKAC	2 cut(s)	186, 270
AciI	CCGC	3 cut(s)	99, 156, 297
AclWI	GGATC	1 cut(s)	137
AcsI	RAATTY	1 cut(s)	66
AcuI	CTGAAG	1 cut(s)	768
AcyI	GRCGYC	1 cut(s)	273
AdeI	CACNNNGTG	1 cut(s)	445
AfaI	GTAC	2 cut(s)	728, 807
AfiI	CCNNNNNNNGG	1 cut(s)	87
AgsI	TTSAA	7 cut(s)	65, 71, 583, 613, 626, 632, 838
AjnI	CCWGG	1 cut(s)	373
AluBI	AGCT	6 cut(s)	120, 175, 431, 617, 746, 867
AluI	AGCT	6 cut(s)	120, 175, 431, 617, 746, 867
Alw21I	GWGCWC	2 cut(s)	177, 404
AlwI	GGATC	1 cut(s)	137
Ama87I	CYCGRG	2 cut(s)	215, 856
AoxI	GGCC	2 cut(s)	298, 594
ApeKI	GCWGC	5 cut(s)	153, 417, 431, 767, 864
ApoI	RAATTY	1 cut(s)	66
ArsI	GACNNNNNNTTYG	2 cut(s)	263, 295
AspLEI	GCGC	1 cut(s)	234
AspS9I	GGNCC	2 cut(s)	10, 311
AsuC2I	CCSGG	3 cut(s)	216, 217, 341
AsuHPI	GGTGA	1 cut(s)	697
AvaI	CYCGRG	2 cut(s)	215, 856
AvaII	GGWCC	2 cut(s)	10, 311
BanII	GRGCYC	1 cut(s)	177
BbsI	GAAGAC	1 cut(s)	84
Bbv12I	GWGCWC	2 cut(s)	177, 404
BbvI	GCAGC	4 cut(s)	140, 418, 429, 754
BccI	CCATC	3 cut(s)	154, 206, 488
BceAI	ACGGC	1 cut(s)	581
BciT130I	CCWGG	1 cut(s)	375
BclI	TGATCA	1 cut(s)	250
BcnI	CCSGG	3 cut(s)	216, 217, 341
BfaI	CTAG	2 cut(s)	203, 666
BfuAI	ACCTGC	1 cut(s)	302
BglI	GCCNNNNNGGC	1 cut(s)	138
BisI	GCNGC	7 cut(s)	154, 157, 298, 418, 432, 768, 865
BlsI	GCNGC	7 cut(s)	155, 158, 299, 419, 433, 769, 866
Bme1390I	CCNGG	4 cut(s)	216, 217, 341, 375
Bme18I	GGWCC	2 cut(s)	10, 311
BmeT110I	CYCGRG	2 cut(s)	215, 856
BmgT120I	GGNCC	2 cut(s)	10, 311
BmiI	GGNNCC	4 cut(s)	292, 371, 450, 549
BmrFI	CCNGG	4 cut(s)	216, 217, 341, 375
BmsI	GCATC	1 cut(s)	334
BoxI	GACNNNNGTC	1 cut(s)	476
BpiI	GAAGAC	1 cut(s)	84
BpuEI	CTTGAG	1 cut(s)	119
BpuMI	CCSGG	3 cut(s)	216, 217, 341
BsaAI	YACGTR	2 cut(s)	229, 645
BsaHI	GRCGYC	1 cut(s)	273
BsaJI	CCNNGG	3 cut(s)	214, 215, 373
BsaWI	WCCGGW	1 cut(s)	387
Bsc4I	CCNNNNNNNGG	1 cut(s)	87
Bse1I	ACTGG	1 cut(s)	761
BseBI	CCWGG	1 cut(s)	375
BseDI	CCNNGG	3 cut(s)	214, 215, 373
BseGI	GGATG	3 cut(s)	146, 198, 349
BseLI	CCNNNNNNNGG	1 cut(s)	87
BseNI	ACTGG	1 cut(s)	761
BseXI	GCAGC	4 cut(s)	140, 418, 429, 754
BsgI	GTGCAG	1 cut(s)	667
BshFI	GGCC	2 cut(s)	300, 596
BsiHKAI	GWGCWC	2 cut(s)	177, 404
BsiHKCI	CYCGRG	2 cut(s)	215, 856
BsiSI	CCGG	5 cut(s)	150, 216, 340, 388, 425
BslFI	GGGAC	1 cut(s)	484
BslI	CCNNNNNNNGG	1 cut(s)	87
BsmFI	GGGAC	1 cut(s)	484
BsnI	GGCC	2 cut(s)	300, 596
BsoBI	CYCGRG	2 cut(s)	215, 856
Bsp1286I	GDGCHC	2 cut(s)	177, 404
Bsp1407I	TGTACA	1 cut(s)	805
Bsp143I	GATC	4 cut(s)	142, 250, 556, 846
BspACI	CCGC	3 cut(s)	99, 156, 297
BspANI	GGCC	2 cut(s)	300, 596
BspLI	GGNNCC	4 cut(s)	292, 371, 450, 549
BspMI	ACCTGC	1 cut(s)	302
BspPI	GGATC	1 cut(s)	137
BsrGI	TGTACA	1 cut(s)	805
BsrI	ACTGG	1 cut(s)	761
BssECI	CCNNGG	3 cut(s)	214, 215, 373
BssMI	GATC	4 cut(s)	142, 250, 556, 846
BssNI	GRCGYC	1 cut(s)	273
Bst2UI	CCWGG	1 cut(s)	375
Bst4CI	ACNGT	5 cut(s)	10, 86, 445, 675, 796
BstACI	GRCGYC	1 cut(s)	273
BstAPI	GCANNNNNTGC	1 cut(s)	654
BstAUI	TGTACA	1 cut(s)	805
BstBAI	YACGTR	2 cut(s)	229, 645
BstC8I	GCNNGC	2 cut(s)	422, 619
BstF5I	GGATG	3 cut(s)	146, 198, 349
BstHHI	GCGC	1 cut(s)	234
BstKTI	GATC	4 cut(s)	145, 253, 559, 849
BstMBI	GATC	4 cut(s)	142, 250, 556, 846
BstMWI	GCNNNNNNNGC	3 cut(s)	49, 138, 654
BstNI	CCWGG	1 cut(s)	375
BstNSI	RCATGY	1 cut(s)	424
BstPAI	GACNNNNGTC	1 cut(s)	476
BstSCI	CCNGG	4 cut(s)	214, 215, 339, 373
BstV1I	GCAGC	4 cut(s)	140, 418, 429, 754
BstV2I	GAAGAC	1 cut(s)	84
BsuRI	GGCC	2 cut(s)	300, 596
BtsCI	GGATG	3 cut(s)	146, 198, 349
BtsIMutI	CAGTG	2 cut(s)	450, 680
BveI	ACCTGC	1 cut(s)	302
Cac8I	GCNNGC	2 cut(s)	422, 619
CfoI	GCGC	1 cut(s)	234
Cfr13I	GGNCC	2 cut(s)	10, 311
Cfr9I	CCCGGG	1 cut(s)	215
Csp6I	GTAC	2 cut(s)	727, 806
CspCI	CAANNNNNGTGG	2 cut(s)	759, 794
CviAII	CATG	4 cut(s)	355, 421, 528, 601
CviQI	GTAC	2 cut(s)	727, 806
DpnI	GATC	4 cut(s)	144, 252, 558, 848
DpnII	GATC	4 cut(s)	142, 250, 556, 846
DraIII	CACNNNGTG	1 cut(s)	445
Ecl136II	GAGCTC	1 cut(s)	175
Eco24I	GRGCYC	1 cut(s)	177
Eco47I	GGWCC	2 cut(s)	10, 311
Eco53kI	GAGCTC	1 cut(s)	175
Eco57I	CTGAAG	1 cut(s)	768
Eco88I	CYCGRG	2 cut(s)	215, 856
EcoICRI	GAGCTC	1 cut(s)	175
EcoRII	CCWGG	1 cut(s)	373
EcoT38I	GRGCYC	1 cut(s)	177
FaeI	CATG	4 cut(s)	358, 424, 531, 604
FaqI	GGGAC	1 cut(s)	484
FatI	CATG	4 cut(s)	354, 420, 527, 600
FbaI	TGATCA	1 cut(s)	250
FblI	GTMKAC	2 cut(s)	186, 270
Fnu4HI	GCNGC	7 cut(s)	154, 157, 298, 418, 432, 768, 865
FokI	GGATG	3 cut(s)	133, 185, 356
FriOI	GRGCYC	1 cut(s)	177
Fsp4HI	GCNGC	7 cut(s)	154, 157, 298, 418, 432, 768, 865
FspBI	CTAG	2 cut(s)	203, 666
FspI	TGCGCA	1 cut(s)	233
GlaI	GCGC	1 cut(s)	233
GluI	GCNGC	7 cut(s)	154, 157, 298, 418, 432, 768, 865
HaeIII	GGCC	2 cut(s)	300, 596
HapII	CCGG	5 cut(s)	150, 216, 340, 388, 425
HhaI	GCGC	1 cut(s)	234
Hin1I	GRCGYC	1 cut(s)	273
Hin1II	CATG	4 cut(s)	358, 424, 531, 604
Hin6I	GCGC	1 cut(s)	232
HinP1I	GCGC	1 cut(s)	232
HincII	GTYRAC	3 cut(s)	187, 271, 277
HindII	GTYRAC	3 cut(s)	187, 271, 277
HinfI	GANTC	3 cut(s)	46, 161, 351
HpaII	CCGG	5 cut(s)	150, 216, 340, 388, 425
HphI	GGTGA	1 cut(s)	697
Hpy166II	GTNNAC	4 cut(s)	187, 271, 277, 671
Hpy188I	TCNGA	1 cut(s)	166
Hpy188III	TCNNGA	1 cut(s)	856
Hpy8I	GTNNAC	4 cut(s)	187, 271, 277, 671
Hpy99I	CGWCG	1 cut(s)	275
HpyAV	CCTTC	2 cut(s)	311, 743
HpyCH4III	ACNGT	5 cut(s)	10, 86, 445, 675, 796
HpyCH4IV	ACGT	4 cut(s)	228, 273, 477, 644
HpyCH4V	TGCA	4 cut(s)	648, 717, 770, 864
HpyF10VI	GCNNNNNNNGC	3 cut(s)	49, 138, 654
HpySE526I	ACGT	4 cut(s)	228, 273, 477, 644
Hsp92I	GRCGYC	1 cut(s)	273
Hsp92II	CATG	4 cut(s)	358, 424, 531, 604
HspAI	GCGC	1 cut(s)	232
Ksp22I	TGATCA	1 cut(s)	250
Kzo9I	GATC	4 cut(s)	142, 250, 556, 846
LmnI	GCTCC	1 cut(s)	553
Lsp1109I	GCAGC	4 cut(s)	140, 418, 429, 754
LweI	GCATC	1 cut(s)	334
MaeI	CTAG	2 cut(s)	203, 666
MaeII	ACGT	4 cut(s)	228, 273, 477, 644
MaeIII	GTNAC	2 cut(s)	571, 640
MalI	GATC	4 cut(s)	144, 252, 558, 848
MboI	GATC	4 cut(s)	142, 250, 556, 846
MboII	GAAGA	3 cut(s)	15, 84, 551
MhlI	GDGCHC	2 cut(s)	177, 404
MluCI	AATT	4 cut(s)	66, 583, 762, 838
MlyI	GAGTC	1 cut(s)	155
MnlI	CCTC	4 cut(s)	143, 528, 586, 776
MspA1I	CMGCKG	1 cut(s)	867
MspI	CCGG	5 cut(s)	150, 216, 340, 388, 425
MspR9I	CCNGG	4 cut(s)	216, 217, 341, 375
MvaI	CCWGG	1 cut(s)	375
MwoI	GCNNNNNNNGC	3 cut(s)	49, 138, 654
NciI	CCSGG	3 cut(s)	216, 217, 341
NdeII	GATC	4 cut(s)	142, 250, 556, 846
NlaIII	CATG	4 cut(s)	358, 424, 531, 604
NlaIV	GGNNCC	4 cut(s)	292, 371, 450, 549
NsbI	TGCGCA	1 cut(s)	233
NspI	RCATGY	1 cut(s)	424
PaeI	GCATGC	1 cut(s)	424
PcsI	WCGNNNNNNNCGW	1 cut(s)	474
PfeI	GAWTC	2 cut(s)	46, 351
PflMI	CCANNNNNTGG	1 cut(s)	87
PfoI	TCCNGGA	1 cut(s)	339
PkrI	GCNGC	7 cut(s)	155, 158, 299, 419, 433, 769, 866
PleI	GAGTC	1 cut(s)	155
PpsI	GAGTC	1 cut(s)	155
Ppu21I	YACGTR	2 cut(s)	229, 645
PshAI	GACNNNNGTC	1 cut(s)	476
Psp124BI	GAGCTC	1 cut(s)	177
Psp6I	CCWGG	1 cut(s)	373
PspGI	CCWGG	1 cut(s)	373
PspN4I	GGNNCC	4 cut(s)	292, 371, 450, 549
PspPI	GGNCC	2 cut(s)	10, 311
PvuII	CAGCTG	1 cut(s)	867
RsaI	GTAC	2 cut(s)	728, 807
RsaNI	GTAC	2 cut(s)	727, 806
SacI	GAGCTC	1 cut(s)	177
SalI	GTCGAC	2 cut(s)	185, 269
SatI	GCNGC	7 cut(s)	154, 157, 298, 418, 432, 768, 865
Sau3AI	GATC	4 cut(s)	142, 250, 556, 846
Sau96I	GGNCC	2 cut(s)	10, 311
SchI	GAGTC	1 cut(s)	155
ScrFI	CCNGG	4 cut(s)	216, 217, 341, 375
SduI	GDGCHC	2 cut(s)	177, 404
SfaNI	GCATC	1 cut(s)	334
SinI	GGWCC	2 cut(s)	10, 311
SmaI	CCCGGG	1 cut(s)	217
SmlI	CTYRAG	1 cut(s)	134
SmoI	CTYRAG	1 cut(s)	134
SphI	GCATGC	1 cut(s)	424
Sse9I	AATT	4 cut(s)	66, 583, 762, 838
SsiI	CCGC	3 cut(s)	99, 156, 297
SspI	AATATT	1 cut(s)	790
SspMI	CTAG	2 cut(s)	203, 666
SstI	GAGCTC	1 cut(s)	177
StyD4I	CCNGG	4 cut(s)	214, 215, 339, 373
TaaI	ACNGT	5 cut(s)	10, 86, 445, 675, 796
TaiI	ACGT	4 cut(s)	231, 276, 480, 647
TaqI	TCGA	6 cut(s)	186, 270, 318, 349, 555, 845
TasI	AATT	4 cut(s)	66, 583, 762, 838
TatI	WGTACW	2 cut(s)	726, 805
TauI	GCSGC	2 cut(s)	159, 300
TfiI	GAWTC	2 cut(s)	46, 351
TscAI	CASTG	2 cut(s)	450, 680
TseI	GCWGC	5 cut(s)	153, 417, 431, 767, 864
TspDTI	ATGAA	1 cut(s)	343
TspGWI	ACGGA	3 cut(s)	251, 294, 303
TspMI	CCCGGG	1 cut(s)	215
TspRI	CASTG	2 cut(s)	450, 680
Van91I	CCANNNNNTGG	1 cut(s)	87
VpaK11BI	GGWCC	2 cut(s)	10, 311
XapI	RAATTY	1 cut(s)	66
XceI	RCATGY	1 cut(s)	424
XmaI	CCCGGG	1 cut(s)	215
XmiI	GTMKAC	2 cut(s)	186, 270
XspI	CTAG	2 cut(s)	203, 666
ZraI	GACGTC	1 cut(s)	274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.