MD16G1035600.v1.1

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004230
Physical Location & Seq
Forward (+)
2560847 .. 2562123
1277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1035600.v1.1.491

Sequence Viewer

Length: 903 bp
ATGAGGACGGTCCAATATACATCTTCTCTTCTCTATTCTCTGCTGATTCTTCTATTTTTCGTTCAAATTCAAGCACAACCCAACTGTTCGTCTTCGTGCGGAGAAATAAAAAACATCAGCTACCCTTTTCGCCTAAAGGGCGATCCATCCGGCTGTGGTGACTCCGATTACGAGCTCTCTTGTGTCGACAACAAAGCCATCCTAGAGATATTCCCCGGGAGATACTACGTGCACAACATTTCCTACCATGATCAAACAATCCGGTTGGTCGACGTCAACTTTGCAAACGGAAGCTGCGGACTTCCGTCTGGTCCTGTCGAAACAACGGACGGGTTCGTCCGGGATGTTCGGTTCAGGGGATATGGGTTGAACCCAGGAAGTCGTTTCCGGTTCGTCAAGTGCTCAACAAACATCAGCAGCGTGCCGGAAGCTGCTAATCACACAACGGTTCCTTGCTTGACAAGAAACGGGACTTACGTCTACGCCGTTTATGATGGCGATTACTCGTATTATGAACCTCGAAAGTCATGCTCTGTCATTTCGTTGGCTCCTGTCGATCTTCGTAAAGATGTTACGAAGTTCAATTCATATGAGGCTGTCATGGAACTATTGAAAGCTGGCTTTGAAATTGGATGGTCAGTTAAGTGCCGAGATTGCTCTTTGGCTGGTAAACAATGCGTAGTAAAATCAATGGATAAGCCACTCATCTATATCTGTGAAAAAGAATACAAAGAACTGACAAGAGCTGAAGGAAACTGGATAATTGCTGCAATCGTTGTGGGAGGTCTGATTGCTCTGGGAGTTATCATTGGTATAATTGTAGTTGTCATCCGAAGATGTCGGAGAAGACGAAATGCAGCAGATAACACGAACAAGAATCTGCAGAATCGGATTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

301

Amino Acids

33.49

Weight (kDa)

8.71

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 91 2.6e-19 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AatII GACGTC 1 cut(s) 276
AccI GTMKAC 3 cut(s) 186, 270, 480
AciI CCGC 2 cut(s) 99, 297
AclWI GGATC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 768
AcyI GRCGYC 1 cut(s) 273
AgsI TTSAA 6 cut(s) 65, 71, 370, 583, 613, 626
AjnI CCWGG 1 cut(s) 373
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 6 cut(s) 120, 175, 294, 431, 617, 746
AluI AGCT 6 cut(s) 120, 175, 294, 431, 617, 746
Alw21I GWGCWC 3 cut(s) 177, 234, 404
Alw44I GTGCAC 1 cut(s) 230
AlwI GGATC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 215
ApaLI GTGCAC 1 cut(s) 230
ApeKI GCWGC 5 cut(s) 294, 417, 431, 767, 857
ApoI RAATTY 1 cut(s) 66
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspS9I GGNCC 2 cut(s) 10, 311
AsuC2I CCSGG 3 cut(s) 216, 217, 341
AsuHPI GGTGA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 10, 311
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 84, 853
Bbv12I GWGCWC 3 cut(s) 177, 234, 404
BbvI GCAGC 5 cut(s) 281, 418, 429, 754, 869
BccI CCATC 4 cut(s) 154, 206, 488, 627
BceAI ACGGC 1 cut(s) 470
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 1 cut(s) 375
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 3 cut(s) 216, 217, 341
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 881
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 5 cut(s) 295, 418, 432, 768, 858
BlsI GCNGC 5 cut(s) 296, 419, 433, 769, 859
Bme1390I CCNGG 4 cut(s) 216, 217, 341, 375
Bme18I GGWCC 2 cut(s) 10, 311
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 10, 311
BmiI GGNNCC 2 cut(s) 450, 549
BmrFI CCNGG 4 cut(s) 216, 217, 341, 375
BmsI GCATC 1 cut(s) 886
BoxI GACNNNNGTC 2 cut(s) 304, 476
BpiI GAAGAC 2 cut(s) 84, 853
BpuMI CCSGG 3 cut(s) 216, 217, 341
BsaAI YACGTR 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 214, 215, 373
BsaWI WCCGGW 2 cut(s) 261, 387
Bse1I ACTGG 1 cut(s) 761
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 3 cut(s) 214, 215, 373
BseGI GGATG 5 cut(s) 146, 198, 349, 638, 828
BseNI ACTGG 1 cut(s) 761
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 5 cut(s) 281, 418, 429, 754, 869
BsiHKAI GWGCWC 3 cut(s) 177, 234, 404
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 6 cut(s) 150, 216, 262, 340, 388, 425
BslFI GGGAC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 484
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 177, 234, 404
Bsp143I GATC 3 cut(s) 142, 250, 556
BspACI CCGC 2 cut(s) 99, 297
BspLI GGNNCC 2 cut(s) 450, 549
BspMAI CTGCAG 1 cut(s) 885
BspPI GGATC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 214, 215, 373
BssMI GATC 3 cut(s) 142, 250, 556
BssNI GRCGYC 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 3 cut(s) 10, 86, 448
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 273
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 422, 619
BstF5I GGATG 5 cut(s) 146, 198, 349, 638, 828
BstKTI GATC 3 cut(s) 145, 253, 559
BstMBI GATC 3 cut(s) 142, 250, 556
BstMWI GCNNNNNNNGC 2 cut(s) 138, 654
BstNI CCWGG 1 cut(s) 375
BstPAI GACNNNNGTC 2 cut(s) 304, 476
BstSCI CCNGG 4 cut(s) 214, 215, 339, 373
BstSFI CTRYAG 1 cut(s) 881
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 5 cut(s) 281, 418, 429, 754, 869
BstV2I GAAGAC 2 cut(s) 84, 853
BtsCI GGATG 5 cut(s) 146, 198, 349, 638, 828
Cac8I GCNNGC 2 cut(s) 422, 619
Cfr13I GGNCC 2 cut(s) 10, 311
Cfr9I CCCGGG 1 cut(s) 215
CspCI CAANNNNNGTGG 2 cut(s) 759, 794
CviAII CATG 3 cut(s) 248, 528, 601
DpnI GATC 3 cut(s) 144, 252, 558
DpnII GATC 3 cut(s) 142, 250, 556
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 2 cut(s) 10, 311
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 177
FaeI CATG 3 cut(s) 251, 531, 604
FaqI GGGAC 1 cut(s) 484
FatI CATG 3 cut(s) 247, 527, 600
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 3 cut(s) 186, 270, 480
Fnu4HI GCNGC 5 cut(s) 295, 418, 432, 768, 858
FokI GGATG 5 cut(s) 133, 185, 356, 645, 815
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 5 cut(s) 295, 418, 432, 768, 858
FspBI CTAG 1 cut(s) 203
GluI GCNGC 5 cut(s) 295, 418, 432, 768, 858
HapII CCGG 6 cut(s) 150, 216, 262, 340, 388, 425
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 3 cut(s) 251, 531, 604
HincII GTYRAC 3 cut(s) 187, 271, 277
HindII GTYRAC 3 cut(s) 187, 271, 277
HinfI GANTC 4 cut(s) 46, 161, 877, 886
HpaII CCGG 6 cut(s) 150, 216, 262, 340, 388, 425
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 6 cut(s) 187, 232, 271, 277, 481, 671
Hpy188I TCNGA 5 cut(s) 166, 789, 833, 843, 891
Hpy8I GTNNAC 6 cut(s) 187, 232, 271, 277, 481, 671
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 743
HpyCH4III ACNGT 3 cut(s) 10, 86, 448
HpyCH4IV ACGT 3 cut(s) 228, 273, 477
HpyCH4V TGCA 5 cut(s) 232, 284, 770, 857, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 654
HpySE526I ACGT 3 cut(s) 228, 273, 477
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 3 cut(s) 251, 531, 604
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 3 cut(s) 142, 250, 556
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 5 cut(s) 281, 418, 429, 754, 869
LweI GCATC 1 cut(s) 886
MaeI CTAG 1 cut(s) 203
MaeII ACGT 3 cut(s) 228, 273, 477
MaeIII GTNAC 2 cut(s) 158, 571
MalI GATC 3 cut(s) 144, 252, 558
MboI GATC 3 cut(s) 142, 250, 556
MboII GAAGA 7 cut(s) 15, 20, 41, 84, 551, 846, 858
MhlI GDGCHC 3 cut(s) 177, 234, 404
MluCI AATT 5 cut(s) 66, 583, 627, 762, 816
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 821
MnlI CCTC 3 cut(s) 528, 586, 776
MseI TTAA 1 cut(s) 642
MspI CCGG 6 cut(s) 150, 216, 262, 340, 388, 425
MspR9I CCNGG 4 cut(s) 216, 217, 341, 375
MvaI CCWGG 1 cut(s) 375
MwoI GCNNNNNNNGC 2 cut(s) 138, 654
NciI CCSGG 3 cut(s) 216, 217, 341
NdeI CATATG 1 cut(s) 589
NdeII GATC 3 cut(s) 142, 250, 556
NlaIII CATG 3 cut(s) 251, 531, 604
NlaIV GGNNCC 2 cut(s) 450, 549
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 3 cut(s) 474, 483, 847
PfeI GAWTC 3 cut(s) 46, 877, 886
PfoI TCCNGGA 1 cut(s) 339
PkrI GCNGC 5 cut(s) 296, 419, 433, 769, 859
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 229
PshAI GACNNNNGTC 2 cut(s) 304, 476
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 2 cut(s) 450, 549
PspPI GGNCC 2 cut(s) 10, 311
PstI CTGCAG 1 cut(s) 885
SacI GAGCTC 1 cut(s) 177
SalI GTCGAC 2 cut(s) 185, 269
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 5 cut(s) 295, 418, 432, 768, 858
Sau3AI GATC 3 cut(s) 142, 250, 556
Sau96I GGNCC 2 cut(s) 10, 311
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 4 cut(s) 216, 217, 341, 375
SduI GDGCHC 3 cut(s) 177, 234, 404
SfaNI GCATC 1 cut(s) 886
SfcI CTRYAG 1 cut(s) 881
SinI GGWCC 2 cut(s) 10, 311
SmaI CCCGGG 1 cut(s) 217
Sse9I AATT 5 cut(s) 66, 583, 627, 762, 816
SsiI CCGC 2 cut(s) 99, 297
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 4 cut(s) 214, 215, 339, 373
TaaI ACNGT 3 cut(s) 10, 86, 448
TaiI ACGT 3 cut(s) 231, 276, 480
TaqI TCGA 5 cut(s) 186, 270, 318, 520, 555
TasI AATT 5 cut(s) 66, 583, 627, 762, 816
TfiI GAWTC 3 cut(s) 46, 877, 886
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 5 cut(s) 294, 417, 431, 767, 857
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 2 cut(s) 528, 576
TspGWI ACGGA 3 cut(s) 294, 303, 341
TspMI CCCGGG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 10, 311
XapI RAATTY 1 cut(s) 66
XmaI CCCGGG 1 cut(s) 215
XmiI GTMKAC 3 cut(s) 186, 270, 480
XspI CTAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.