RchiOBHm_Chr4g0434121

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004230
Physical Location & Seq
Forward (+)
58001488 .. 58002453
966 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40259

Sequence Viewer

Length: 966 bp
ATGTTTTTTTTTTTTTTTTTTTTAAAATTCTTGTTCAGTATATATATAGAGCTGCTGTGTAACATTAGGCTAATCCAAGTTCTCAACTTCATCATGAAGAGTCTGAGGAAAGAAGAATCAGCAGCTGCATCCATTCTCCTCTATTCTGTGCTGACTTCTCTATTTTTCCTTGCAGTTCAAGCAAGAAGTCATAAACCAGGGTGTGCTTCTTCGTGTGGAGATATCGAAAACATCAGCTACCCTTTTCGCCTAAAGGTTGATCCAGTAGGCTGCGGTGACCCTGACTATGAACTATCATGTGTGAACAACAAAACCATCCTCGAAATATTTCCAGGCAAATACTATGTCAAGAACATTTCTTATGATGAACATATTATCCGTTTGGTTGATGTCAACTTTGCCGATAGAAGCAGCTGCAGCCTTCCATCGGGATCTGTTGTATCGAGGGATGGATATGTCAATGATTTTCGCTTCTTGGCACTTGGCACAAATCAAACTTACACACAGTTTGTGAAGTGCTCCAGAAACATCAGCCAGGCTAATGTAGCTGCTTACAACTACACGGCTGTTCCATGTTTAACTAGAGTTAATGGGACTAATTACTTCTATGCTGTTTATGATGGCTACTACTTAGCTCGTCCTCGTCAACCACCATGCTCGCTTATTTCAGTGGCTATTTCAGATTATCATGAAAATTTTGTTGAGCCCCTATCTTATGAAGCCATCATGAAGCAATTGGAGGCAGGGTTTGATCTGGGATGGTCCGTTGAGTGTCGCGACTGCTCTTTAGCTGGCAAATCATGTGGAGTAGATGTAAGTGATCTAAATTTGGGTACTAAGCCACTCCCCTATCAATGTTATGAAGGTCAGTTTACTATAATATGCCTATTCTATCTTTATCATGAAGGTCAGTTTACTATATATCATACTATATTCTACAGGTACAGAGTACAAAGAACCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

321

Amino Acids

36.71

Weight (kDa)

6.58

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 68 - 130 1.1e-19 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 777
AciI CCGC 1 cut(s) 273
AclWI GGATC 2 cut(s) 254, 439
AcsI RAATTY 3 cut(s) 26, 694, 826
AfaI GTAC 3 cut(s) 835, 944, 951
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 3 cut(s) 196, 331, 534
AloI GAACNNNNNNTCC 2 cut(s) 360, 392
AluBI AGCT 7 cut(s) 52, 125, 237, 414, 548, 635, 791
AluI AGCT 7 cut(s) 52, 125, 237, 414, 548, 635, 791
Alw21I GWGCWC 1 cut(s) 521
AlwI GGATC 2 cut(s) 254, 439
AlwNI CAGNNNCTG 1 cut(s) 125
ApeKI GCWGC 8 cut(s) 52, 122, 125, 270, 411, 414, 417, 548
ApoI RAATTY 3 cut(s) 26, 694, 826
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
Asp700I GAANNNNTTC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 762
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 762
BanII GRGCYC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 8 cut(s) 39, 112, 134, 257, 401, 423, 429, 535
BccI CCATC 6 cut(s) 323, 433, 443, 614, 731, 753
BceAI ACGGC 1 cut(s) 579
BciT130I CCWGG 3 cut(s) 198, 333, 536
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 2 cut(s) 415, 937
BisI GCNGC 8 cut(s) 53, 123, 126, 271, 412, 415, 418, 549
BlsI GCNGC 8 cut(s) 54, 124, 127, 272, 413, 416, 419, 550
Bme1390I CCNGG 3 cut(s) 198, 333, 536
Bme18I GGWCC 1 cut(s) 762
BmgT120I GGNCC 1 cut(s) 762
BmrFI CCNGG 3 cut(s) 198, 333, 536
BmsI GCATC 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 197
BsaXI ACNNNNNCTCC 4 cut(s) 731, 761, 798, 828
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 1 cut(s) 263
BseBI CCWGG 3 cut(s) 198, 333, 536
BseDI CCNNGG 1 cut(s) 197
BseGI GGATG 4 cut(s) 128, 315, 454, 764
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 8 cut(s) 39, 112, 134, 257, 401, 423, 429, 535
Bsh1236I CGCG 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 521
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 1 cut(s) 427
BsmFI GGGAC 1 cut(s) 607
Bsp1286I GDGCHC 2 cut(s) 521, 708
Bsp143I GATC 4 cut(s) 259, 431, 751, 820
Bsp68I TCGCGA 1 cut(s) 777
BspACI CCGC 1 cut(s) 273
BspCNI CTCAG 1 cut(s) 96
BspFNI CGCG 1 cut(s) 777
BspHI TCATGA 4 cut(s) 93, 688, 726, 901
BspMAI CTGCAG 1 cut(s) 419
BspPI GGATC 2 cut(s) 254, 439
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 197
BssMI GATC 4 cut(s) 259, 431, 751, 820
Bst2UI CCWGG 3 cut(s) 198, 333, 536
Bst4CI ACNGT 1 cut(s) 507
Bst6I CTCTTC 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 659, 793
BstDEI CTNAG 3 cut(s) 104, 631, 837
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 4 cut(s) 128, 315, 454, 764
BstFNI CGCG 1 cut(s) 777
BstKTI GATC 4 cut(s) 262, 434, 754, 823
BstMBI GATC 4 cut(s) 259, 431, 751, 820
BstMWI GCNNNNNNNGC 3 cut(s) 179, 417, 545
BstNI CCWGG 3 cut(s) 198, 333, 536
BstPI GGTNACC 1 cut(s) 275
BstSCI CCNGG 3 cut(s) 196, 331, 534
BstSFI CTRYAG 2 cut(s) 415, 937
BstUI CGCG 1 cut(s) 777
BstV1I GCAGC 8 cut(s) 39, 112, 134, 257, 401, 423, 429, 535
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BtsCI GGATG 4 cut(s) 128, 315, 454, 764
BtsIMutI CAGTG 2 cut(s) 675, 961
BtuMI TCGCGA 1 cut(s) 777
Cac8I GCNNGC 2 cut(s) 659, 793
CaiI CAGNNNCTG 1 cut(s) 125
CciI TCATGA 4 cut(s) 93, 688, 726, 901
Cfr13I GGNCC 1 cut(s) 762
Csp6I GTAC 3 cut(s) 834, 943, 950
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 8 cut(s) 94, 297, 573, 654, 689, 727, 801, 902
CviQI GTAC 3 cut(s) 834, 943, 950
DdeI CTNAG 3 cut(s) 104, 631, 837
DpnI GATC 4 cut(s) 261, 433, 753, 822
DpnII GATC 4 cut(s) 259, 431, 751, 820
DraI TTTAAA 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 708
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 762
Eco91I GGTNACC 1 cut(s) 275
EcoO65I GGTNACC 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 196, 331, 534
EcoRV GATATC 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 708
FaeI CATG 8 cut(s) 97, 300, 576, 657, 692, 730, 804, 905
FaqI GGGAC 1 cut(s) 607
FatI CATG 8 cut(s) 93, 296, 572, 653, 688, 726, 800, 901
Fnu4HI GCNGC 8 cut(s) 53, 123, 126, 271, 412, 415, 418, 549
FokI GGATG 4 cut(s) 115, 302, 461, 771
FriOI GRGCYC 1 cut(s) 708
Fsp4HI GCNGC 8 cut(s) 53, 123, 126, 271, 412, 415, 418, 549
FspBI CTAG 1 cut(s) 582
GluI GCNGC 8 cut(s) 53, 123, 126, 271, 412, 415, 418, 549
GsuI CTGGAG 1 cut(s) 505
Hin1II CATG 8 cut(s) 97, 300, 576, 657, 692, 730, 804, 905
HincII GTYRAC 2 cut(s) 394, 647
HindII GTYRAC 2 cut(s) 394, 647
HinfI GANTC 2 cut(s) 100, 116
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 5 cut(s) 304, 394, 647, 873, 915
Hpy188I TCNGA 2 cut(s) 105, 682
Hpy188III TCNNGA 8 cut(s) 94, 349, 429, 522, 689, 727, 776, 902
Hpy8I GTNNAC 5 cut(s) 304, 394, 647, 873, 915
HpyAV CCTTC 3 cut(s) 431, 857, 899
HpyCH4III ACNGT 1 cut(s) 507
HpyCH4V TGCA 3 cut(s) 128, 173, 417
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 417, 545
HpyF3I CTNAG 3 cut(s) 104, 631, 837
Hsp92II CATG 8 cut(s) 97, 300, 576, 657, 692, 730, 804, 905
Kzo9I GATC 4 cut(s) 259, 431, 751, 820
LmnI GCTCC 1 cut(s) 524
Lsp1109I GCAGC 8 cut(s) 39, 112, 134, 257, 401, 423, 429, 535
LweI GCATC 1 cut(s) 137
MaeI CTAG 1 cut(s) 582
MaeIII GTNAC 2 cut(s) 59, 275
MalI GATC 4 cut(s) 261, 433, 753, 822
MboI GATC 4 cut(s) 259, 431, 751, 820
MboII GAAGA 3 cut(s) 109, 125, 201
MfeI CAATTG 1 cut(s) 734
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 2 cut(s) 521, 708
MluCI AATT 5 cut(s) 26, 598, 694, 734, 826
MlyI GAGTC 1 cut(s) 109
MnlI CCTC 6 cut(s) 99, 149, 329, 438, 651, 733
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 3 cut(s) 23, 578, 588
MspA1I CMGCKG 2 cut(s) 125, 414
MspR9I CCNGG 3 cut(s) 198, 333, 536
MunI CAATTG 1 cut(s) 734
MvaI CCWGG 3 cut(s) 198, 333, 536
MvnI CGCG 1 cut(s) 777
MwoI GCNNNNNNNGC 3 cut(s) 179, 417, 545
NdeII GATC 4 cut(s) 259, 431, 751, 820
NlaIII CATG 8 cut(s) 97, 300, 576, 657, 692, 730, 804, 905
NmuCI GTSAC 1 cut(s) 275
NruI TCGCGA 1 cut(s) 777
PagI TCATGA 4 cut(s) 93, 688, 726, 901
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 8 cut(s) 54, 124, 127, 272, 413, 416, 419, 550
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp6I CCWGG 3 cut(s) 196, 331, 534
PspEI GGTNACC 1 cut(s) 275
PspGI CCWGG 3 cut(s) 196, 331, 534
PspPI GGNCC 1 cut(s) 762
PstI CTGCAG 1 cut(s) 419
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 1 cut(s) 431
PvuII CAGCTG 2 cut(s) 125, 414
RruI TCGCGA 1 cut(s) 777
RsaI GTAC 3 cut(s) 835, 944, 951
RsaNI GTAC 3 cut(s) 834, 943, 950
SaqAI TTAA 3 cut(s) 23, 578, 588
SatI GCNGC 8 cut(s) 53, 123, 126, 271, 412, 415, 418, 549
Sau3AI GATC 4 cut(s) 259, 431, 751, 820
Sau96I GGNCC 1 cut(s) 762
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 3 cut(s) 198, 333, 536
SduI GDGCHC 2 cut(s) 521, 708
SfaNI GCATC 1 cut(s) 137
SfcI CTRYAG 2 cut(s) 415, 937
SinI GGWCC 1 cut(s) 762
Sse9I AATT 5 cut(s) 26, 598, 694, 734, 826
SsiI CCGC 1 cut(s) 273
SspI AATATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 582
StyD4I CCNGG 3 cut(s) 196, 331, 534
TaaI ACNGT 1 cut(s) 507
TaqI TCGA 3 cut(s) 225, 321, 443
TasI AATT 5 cut(s) 26, 598, 694, 734, 826
TatI WGTACW 1 cut(s) 949
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 3 cut(s) 23, 578, 588
Tru9I TTAA 3 cut(s) 23, 578, 588
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 8 cut(s) 52, 122, 125, 270, 411, 414, 417, 548
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 9 cut(s) 79, 110, 303, 381, 705, 732, 743, 876, 918
TspGWI ACGGA 2 cut(s) 368, 754
TspRI CASTG 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 762
XapI RAATTY 3 cut(s) 26, 694, 826
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.