Rh4BG336900

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004230
Physical Location & Seq
Forward (+)
50010931 .. 50021715
10785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG336900.1

Sequence Viewer

Length: 978 bp
ATGAAGAGTCTGAGGAAAGAAGAATCAGCAGATGCATCCATTCTCCTCTATTCTATGCTGACTTCTCTATTTTTCCTTGCAGTTCAAGCAAGAAGTCATAAACCAGCGTGTGCTTCTTCGTGTGGAGATATCGAAAACATCAGCTACCCTTTTCGCCTAAAGGTTGATCCAGTAGGCTGCGGTGACCCTGACTATGAACTATCATGTGTGAACAACAAAACCATCCTCGAAATATTTCCAGGCAAATACTATGTCAAGAACATTTCTTATGATGAACATATTATCCGTTTGGTTGATGTCAACTTTGCCAATGGAAGCAGCTGCAGCCTTCCATCGGGATCTGTTGTATCGAGGGATGGATATGTCAATGATTTTCGCTTCTTGGCACTTGGCACAAATCAAACTTACACACAGTTTGTGAAGTGCTCCAGAAACATCAGCCAGGCTAATGTAGCTGCTTATAACTACACGGCAGTTCCATGTTTAACTAGAGTTAATGGGACTAATTACTTCTATGCTGTTTATGATGGCTACTACTTAGCTCGTCCTCGTCAACCACCATGCTCGCTTATTTCAGTGGCTATTTCAGATTATCATGAAAATTTTGTTGAGCCCCTATCTTATGAAGCCATCATGAAGCAATTGGCGGCAGGGTTTGATCTGGGATGGTCCGTTGAGTGTCGCGACTGCTCTTTAGCTGGAAAATCATGTGGAGTAGATGTAAGTGATCTAAATTTGGGTACTAAGCCACTCCCCTATCAATGTTATGAAGGTACAGAGTACAAAGAACCCACTGAGCTCCAAATAATCTTAATATTTGTGGGAATTGCTTTGGGAGGTATAAATTATATAAGTTGCCTTACACTTTCTGAAAGACATATTTTATTGTGTTTCAGAATCTCAGATAGCGTGTCACCTCCAAAACAGAACCACGACGCGTCTAGGCAACTGGCTGGTATACTGGTAAACACCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

325

Amino Acids

35.99

Weight (kDa)

5.45

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 99 1.1e-19 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 462
AccI GTMKAC 1 cut(s) 958
AccII CGCG 2 cut(s) 684, 938
AciI CCGC 2 cut(s) 180, 647
AclWI GGATC 2 cut(s) 161, 346
AcsI RAATTY 2 cut(s) 601, 733
AfaI GTAC 3 cut(s) 742, 775, 782
AfiI CCNNNNNNNGG 1 cut(s) 334
AflIII ACRYGT 1 cut(s) 936
AgsI TTSAA 1 cut(s) 86
AjnI CCWGG 2 cut(s) 238, 441
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 6 cut(s) 144, 321, 455, 542, 698, 799
AluI AGCT 6 cut(s) 144, 321, 455, 542, 698, 799
Alw21I GWGCWC 2 cut(s) 428, 801
AlwI GGATC 2 cut(s) 161, 346
ApeKI GCWGC 5 cut(s) 177, 318, 321, 324, 455
ApoI RAATTY 2 cut(s) 601, 733
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
Asp700I GAANNNNTTC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 669
AsuHPI GGTGA 2 cut(s) 194, 906
AvaII GGWCC 1 cut(s) 669
BanII GRGCYC 2 cut(s) 615, 801
Bbv12I GWGCWC 2 cut(s) 428, 801
BbvI GCAGC 5 cut(s) 164, 308, 330, 336, 442
BccI CCATC 6 cut(s) 230, 340, 350, 521, 638, 660
BceAI ACGGC 1 cut(s) 486
BciT130I CCWGG 2 cut(s) 240, 443
BfaI CTAG 2 cut(s) 489, 942
BfmI CTRYAG 1 cut(s) 322
BisI GCNGC 6 cut(s) 178, 319, 322, 325, 456, 648
BlsI GCNGC 6 cut(s) 179, 320, 323, 326, 457, 649
Bme1390I CCNGG 2 cut(s) 240, 443
Bme18I GGWCC 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 669
BmrFI CCNGG 2 cut(s) 240, 443
BmsI GCATC 2 cut(s) 22, 44
BpmI CTGGAG 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 3 cut(s) 170, 954, 966
BseBI CCWGG 2 cut(s) 240, 443
BseGI GGATG 4 cut(s) 35, 222, 361, 671
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMII CTCAG 2 cut(s) 786, 915
BseNI ACTGG 3 cut(s) 170, 954, 966
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 5 cut(s) 164, 308, 330, 336, 442
Bsh1236I CGCG 2 cut(s) 684, 938
BsiHKAI GWGCWC 2 cut(s) 428, 801
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 334
BsmFI GGGAC 1 cut(s) 514
Bsp1286I GDGCHC 3 cut(s) 428, 615, 801
Bsp143I GATC 4 cut(s) 166, 338, 658, 727
Bsp68I TCGCGA 1 cut(s) 684
BspACI CCGC 2 cut(s) 180, 647
BspCNI CTCAG 2 cut(s) 787, 914
BspFNI CGCG 2 cut(s) 684, 938
BspHI TCATGA 2 cut(s) 595, 633
BspMAI CTGCAG 1 cut(s) 326
BspPI GGATC 2 cut(s) 161, 346
BsrI ACTGG 3 cut(s) 170, 954, 966
BssMI GATC 4 cut(s) 166, 338, 658, 727
BssNAI GTATAC 1 cut(s) 959
Bst1107I GTATAC 1 cut(s) 959
Bst2UI CCWGG 2 cut(s) 240, 443
Bst4CI ACNGT 1 cut(s) 414
BstC8I GCNNGC 1 cut(s) 566
BstDEI CTNAG 5 cut(s) 11, 538, 744, 795, 901
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 4 cut(s) 35, 222, 361, 671
BstFNI CGCG 2 cut(s) 684, 938
BstKTI GATC 4 cut(s) 169, 341, 661, 730
BstMBI GATC 4 cut(s) 166, 338, 658, 727
BstMWI GCNNNNNNNGC 3 cut(s) 86, 324, 452
BstNI CCWGG 2 cut(s) 240, 443
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 238, 441
BstSFI CTRYAG 1 cut(s) 322
BstUI CGCG 2 cut(s) 684, 938
BstV1I GCAGC 5 cut(s) 164, 308, 330, 336, 442
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BstZ17I GTATAC 1 cut(s) 959
BtsCI GGATG 4 cut(s) 35, 222, 361, 671
BtsIMutI CAGTG 2 cut(s) 582, 792
BtuMI TCGCGA 1 cut(s) 684
Cac8I GCNNGC 1 cut(s) 566
CciI TCATGA 2 cut(s) 595, 633
Cfr13I GGNCC 1 cut(s) 669
CseI GACGC 2 cut(s) 927, 944
Csp6I GTAC 3 cut(s) 741, 774, 781
CviAII CATG 6 cut(s) 204, 480, 561, 596, 634, 708
CviQI GTAC 3 cut(s) 741, 774, 781
DdeI CTNAG 5 cut(s) 11, 538, 744, 795, 901
DpnI GATC 4 cut(s) 168, 340, 660, 729
DpnII GATC 4 cut(s) 166, 338, 658, 727
Ecl136II GAGCTC 1 cut(s) 799
Eco24I GRGCYC 2 cut(s) 615, 801
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 669
Eco53kI GAGCTC 1 cut(s) 799
Eco91I GGTNACC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 799
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 238, 441
EcoRV GATATC 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 37
EcoT38I GRGCYC 2 cut(s) 615, 801
FaeI CATG 6 cut(s) 207, 483, 564, 599, 637, 711
FaqI GGGAC 1 cut(s) 514
FatI CATG 6 cut(s) 203, 479, 560, 595, 633, 707
FblI GTMKAC 1 cut(s) 958
Fnu4HI GCNGC 6 cut(s) 178, 319, 322, 325, 456, 648
FokI GGATG 4 cut(s) 22, 209, 368, 678
FriOI GRGCYC 2 cut(s) 615, 801
Fsp4HI GCNGC 6 cut(s) 178, 319, 322, 325, 456, 648
FspBI CTAG 2 cut(s) 489, 942
GluI GCNGC 6 cut(s) 178, 319, 322, 325, 456, 648
GsuI CTGGAG 1 cut(s) 412
HgaI GACGC 2 cut(s) 927, 944
Hin1II CATG 6 cut(s) 207, 483, 564, 599, 637, 711
HincII GTYRAC 2 cut(s) 301, 554
HindII GTYRAC 2 cut(s) 301, 554
HinfI GANTC 3 cut(s) 7, 23, 897
HphI GGTGA 2 cut(s) 194, 906
Hpy166II GTNNAC 5 cut(s) 211, 301, 554, 959, 967
Hpy188I TCNGA 5 cut(s) 12, 589, 871, 896, 904
Hpy188III TCNNGA 6 cut(s) 256, 336, 429, 596, 634, 683
Hpy8I GTNNAC 5 cut(s) 211, 301, 554, 959, 967
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 2 cut(s) 338, 764
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4V TGCA 3 cut(s) 35, 80, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 324, 452
HpyF3I CTNAG 5 cut(s) 11, 538, 744, 795, 901
Hsp92II CATG 6 cut(s) 207, 483, 564, 599, 637, 711
Kzo9I GATC 4 cut(s) 166, 338, 658, 727
LmnI GCTCC 2 cut(s) 431, 804
Lsp1109I GCAGC 5 cut(s) 164, 308, 330, 336, 442
LweI GCATC 2 cut(s) 22, 44
MaeI CTAG 2 cut(s) 489, 942
MaeIII GTNAC 2 cut(s) 182, 912
MalI GATC 4 cut(s) 168, 340, 660, 729
MboI GATC 4 cut(s) 166, 338, 658, 727
MboII GAAGA 3 cut(s) 16, 32, 108
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 3 cut(s) 428, 615, 801
MluCI AATT 6 cut(s) 505, 601, 641, 733, 825, 844
MluI ACGCGT 1 cut(s) 936
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 7 cut(s) 6, 56, 236, 345, 558, 830, 927
Mph1103I ATGCAT 1 cut(s) 37
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 3 cut(s) 485, 495, 812
MspA1I CMGCKG 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 240, 443
MunI CAATTG 1 cut(s) 641
MvaI CCWGG 2 cut(s) 240, 443
MvnI CGCG 2 cut(s) 684, 938
MwoI GCNNNNNNNGC 3 cut(s) 86, 324, 452
NdeII GATC 4 cut(s) 166, 338, 658, 727
NlaIII CATG 6 cut(s) 207, 483, 564, 599, 637, 711
NmuCI GTSAC 2 cut(s) 182, 912
NruI TCGCGA 1 cut(s) 684
NsiI ATGCAT 1 cut(s) 37
PagI TCATGA 2 cut(s) 595, 633
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 2 cut(s) 23, 897
PkrI GCNGC 6 cut(s) 179, 320, 323, 326, 457, 649
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PsiI TTATAA 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 801
Psp6I CCWGG 2 cut(s) 238, 441
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 238, 441
PspPI GGNCC 1 cut(s) 669
PstI CTGCAG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 321
RruI TCGCGA 1 cut(s) 684
RsaI GTAC 3 cut(s) 742, 775, 782
RsaNI GTAC 3 cut(s) 741, 774, 781
SacI GAGCTC 1 cut(s) 801
SaqAI TTAA 3 cut(s) 485, 495, 812
SatI GCNGC 6 cut(s) 178, 319, 322, 325, 456, 648
Sau3AI GATC 4 cut(s) 166, 338, 658, 727
Sau96I GGNCC 1 cut(s) 669
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 240, 443
SduI GDGCHC 3 cut(s) 428, 615, 801
SfaNI GCATC 2 cut(s) 22, 44
SfcI CTRYAG 1 cut(s) 322
SinI GGWCC 1 cut(s) 669
Sse9I AATT 6 cut(s) 505, 601, 641, 733, 825, 844
SsiI CCGC 2 cut(s) 180, 647
SspI AATATT 2 cut(s) 234, 816
SspMI CTAG 2 cut(s) 489, 942
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 2 cut(s) 238, 441
TaaI ACNGT 1 cut(s) 414
TaqI TCGA 3 cut(s) 132, 228, 350
TasI AATT 6 cut(s) 505, 601, 641, 733, 825, 844
TatI WGTACW 1 cut(s) 780
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 2 cut(s) 23, 897
Tru1I TTAA 3 cut(s) 485, 495, 812
Tru9I TTAA 3 cut(s) 485, 495, 812
TscAI CASTG 2 cut(s) 582, 799
TseFI GTSAC 2 cut(s) 182, 912
TseI GCWGC 5 cut(s) 177, 318, 321, 324, 455
Tsp45I GTSAC 2 cut(s) 182, 912
TspDTI ATGAA 7 cut(s) 17, 210, 288, 612, 639, 650, 783
TspGWI ACGGA 2 cut(s) 275, 661
TspRI CASTG 2 cut(s) 582, 799
VpaK11BI GGWCC 1 cut(s) 669
XapI RAATTY 2 cut(s) 601, 733
XmiI GTMKAC 1 cut(s) 958
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 2 cut(s) 489, 942
Zsp2I ATGCAT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.