FvH4_4g30650

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002719
Physical Location & Seq
Reverse (-)
30179162 .. 30182901
3740 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30650.t4

Sequence Viewer

Length: 1506 bp
ATGGGTGTTTGTGTCTCAAAGCCAAGTGAGGCTCTCAGAGCACAGACGAAAAGAGCCGAGAGACACGGCAGAGGCAGACTCCGACGCCGGGGGAGGAAGAGCAAGAAGGTAGCCTCCACCGTTATAAGCTCGGAGATTGCTGCTCCGACGAGACGGTCTGTTAGTGAGTTTGTGCATCTAAACTTCGAGAAAGGTGCTGCAACCACTTGCAAAAGATCTGAGGTTTCCAACGGCACATATCATCTAACTCAGCTTCAATGGAATCACAGTCAGATTGATGCCAATGGTGGGAAATGCCAAGGACCTGAAACATGGTTCGACTCGCATAGTATAATGGATTCTGATTCAGATGATGACTTTTCTAGTACACAAGGAGACACATTTCCTCCTACGATAGTAGGTAATGGAATTGGAAACATCCCAAATAGTCAATTGGTCCAGTATGAAAGTGCAGCATGTATTGTAGATAATGGAGGCAAGTATGAAGGGTTTTATGAAAGTTATCTGAAAATTGATGGTCCAGGTGCACGACATTCATCAGAGAAAACACAAGTCAGTTACAATGACAATTATCAGCCCACGCATTCCGCAGTTATAATGCTTTCATATAAGAGAAAATCCATTGACAACCCTGATAGGACATCATGTGCTTCTGATCAGAAGTACTTATTCCGTCCTAAAGCAGGACAACGCATTCCTTGTTCACCGATAGAGAAGACAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTCAAGCTACGCGGGGAGAACTATTTCAAGGACAGACAGAAGTACCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTGTTGATATGTTTATCTGCCCAAAGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAAAACCACATGACAAAGTTCCTTCCCTCCTCATTGTTAATATACAGTTGCCTACATATCCTACTTCAATGTTCCTTGGGGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTTAGTGAAAGTTTTGACACAGACATATCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGGATGACAGCGAAACGGTTAAGGGGTTCACATCGAACTCATCAGTTCCTTTCAGGGAAAGGCTAAAAATTTTGGCTGGATTGGTGAATCCAGACGAGCTCCAACTGAGTGCTGCTGAGAAGAAGCTTATTAATGCTTATAATGATAAACCAGTGCTTTCCCGCCCTCAACACAATTTCTTCAGGGGGCCTAATTACTTTGAGATTGACCTAGACATACACAGGTTCAGCTACATCTCTAGGAAAGGGCTTGAATCATTCAGAGACAGATTGAAGAATGGGGTAGTTGATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAACTACCTGAGCAAGTCTTGTGCTGTATGCAGTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

502

Amino Acids

56.42

Weight (kDa)

7.94

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 188 - 429 7.1e-62 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 125, 596, 1227
AasI GACNNNNNNGTC 1 cut(s) 154
AccII CGCG 1 cut(s) 768
AciI CCGC 3 cut(s) 588, 768, 1249
AclWI GGATC 2 cut(s) 723, 736
AcsI RAATTY 2 cut(s) 1067, 1155
AcuI CTGAAG 2 cut(s) 735, 1252
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 4 cut(s) 367, 665, 800, 828
AfiI CCNNNNNNNGG 3 cut(s) 88, 288, 683
AgsI TTSAA 8 cut(s) 257, 760, 784, 969, 1064, 1340, 1360, 1447
AjnI CCWGG 4 cut(s) 520, 724, 731, 1392
AluBI AGCT 8 cut(s) 129, 253, 763, 819, 892, 1186, 1213, 1317
AluI AGCT 8 cut(s) 129, 253, 763, 819, 892, 1186, 1213, 1317
Alw21I GWGCWC 3 cut(s) 43, 529, 1188
Alw26I GTCTC 5 cut(s) 19, 55, 145, 369, 1344
Alw44I GTGCAC 1 cut(s) 525
AlwI GGATC 2 cut(s) 723, 736
AoxI GGCC 1 cut(s) 1274
ApaLI GTGCAC 1 cut(s) 525
ApeKI GCWGC 4 cut(s) 140, 197, 452, 1199
ApoI RAATTY 2 cut(s) 1067, 1155
AseI ATTAAT 1 cut(s) 1218
Asp700I GAANNNNTTC 1 cut(s) 779
AspS9I GGNCC 4 cut(s) 302, 436, 518, 1274
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 3 cut(s) 696, 1004, 1183
AvaII GGWCC 3 cut(s) 302, 436, 518
BaeGI GKGCMC 1 cut(s) 529
BaeI ACNNNNGTAYC 2 cut(s) 782, 815
BamHI GGATCC 1 cut(s) 728
BanII GRGCYC 1 cut(s) 1188
BbsI GAAGAC 2 cut(s) 722, 748
Bbv12I GWGCWC 3 cut(s) 43, 529, 1188
BbvI GCAGC 4 cut(s) 127, 184, 464, 1186
BccI CCATC 2 cut(s) 509, 983
BceAI ACGGC 2 cut(s) 82, 247
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 4 cut(s) 522, 726, 733, 1394
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 5 cut(s) 19, 55, 145, 369, 1344
BfaI CTAG 3 cut(s) 363, 1298, 1326
BfmI CTRYAG 1 cut(s) 814
BglII AGATCT 1 cut(s) 215
BisI GCNGC 4 cut(s) 141, 198, 453, 1200
BlpI GCTNAGC 1 cut(s) 879
BlsI GCNGC 4 cut(s) 142, 199, 454, 1201
BmcAI AGTACT 1 cut(s) 665
Bme1390I CCNGG 5 cut(s) 89, 522, 726, 733, 1394
Bme18I GGWCC 3 cut(s) 302, 436, 518
BmgT120I GGNCC 4 cut(s) 302, 436, 518, 1274
BmiI GGNNCC 2 cut(s) 730, 1275
BmrFI CCNGG 5 cut(s) 89, 522, 726, 733, 1394
BmsI GCATC 3 cut(s) 184, 268, 892
BpiI GAAGAC 2 cut(s) 722, 748
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 1 cut(s) 708
Bpu10I CCTNAGC 1 cut(s) 1419
Bpu1102I GCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 908
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 5 cut(s) 88, 298, 976, 1468, 1494
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 288, 683
Bse1I ACTGG 2 cut(s) 439, 1238
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 4 cut(s) 522, 726, 733, 1394
BseDI CCNNGG 5 cut(s) 88, 298, 976, 1468, 1494
BseGI GGATG 3 cut(s) 417, 1096, 1504
BseLI CCNNNNNNNGG 3 cut(s) 88, 288, 683
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 8 cut(s) 49, 210, 263, 893, 1070, 1184, 1194, 1410
BseNI ACTGG 2 cut(s) 439, 1238
BseRI GAGGAG 2 cut(s) 920, 1044
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 4 cut(s) 127, 184, 464, 1186
BsgI GTGCAG 1 cut(s) 471
Bsh1236I CGCG 1 cut(s) 768
BshFI GGCC 1 cut(s) 1276
BsiHKAI GWGCWC 3 cut(s) 43, 529, 1188
BsiSI CCGG 1 cut(s) 88
BslI CCNNNNNNNGG 3 cut(s) 88, 288, 683
BsmAI GTCTC 5 cut(s) 19, 55, 145, 369, 1344
BsmBI CGTCTC 1 cut(s) 145
BsmI GAATGC 2 cut(s) 583, 693
BsnI GGCC 1 cut(s) 1276
Bsp1286I GDGCHC 3 cut(s) 43, 529, 1188
Bsp143I GATC 4 cut(s) 215, 655, 728, 868
Bsp1720I GCTNAGC 1 cut(s) 879
Bsp19I CCATGG 2 cut(s) 1468, 1494
BspACI CCGC 3 cut(s) 588, 768, 1249
BspANI GGCC 1 cut(s) 1276
BspCNI CTCAG 8 cut(s) 48, 211, 262, 892, 1069, 1185, 1195, 1411
BspFNI CGCG 1 cut(s) 768
BspLI GGNNCC 2 cut(s) 730, 1275
BspPI GGATC 2 cut(s) 723, 736
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 873
BsrI ACTGG 2 cut(s) 439, 1238
BssECI CCNNGG 5 cut(s) 88, 298, 976, 1468, 1494
BssMI GATC 4 cut(s) 215, 655, 728, 868
BssNI GRCGYC 1 cut(s) 85
BssT1I CCWWGG 4 cut(s) 298, 976, 1468, 1494
Bst2UI CCWGG 4 cut(s) 522, 726, 733, 1394
Bst4CI ACNGT 5 cut(s) 121, 156, 269, 948, 1105
Bst6I CTCTTC 2 cut(s) 92, 857
BstACI GRCGYC 1 cut(s) 85
BstDEI CTNAG 8 cut(s) 35, 219, 249, 879, 1056, 1193, 1203, 1419
BstDSI CCRYGG 2 cut(s) 1468, 1494
BstENI CCTNNNNNAGG 1 cut(s) 681
BstF5I GGATG 3 cut(s) 417, 1096, 1504
BstFNI CGCG 1 cut(s) 768
BstKTI GATC 4 cut(s) 218, 658, 731, 871
BstMAI GTCTC 5 cut(s) 19, 55, 145, 369, 1344
BstMBI GATC 4 cut(s) 215, 655, 728, 868
BstMWI GCNNNNNNNGC 2 cut(s) 38, 889
BstNI CCWGG 4 cut(s) 522, 726, 733, 1394
BstNSI RCATGY 1 cut(s) 459
BstSCI CCNGG 5 cut(s) 87, 520, 724, 731, 1392
BstSFI CTRYAG 1 cut(s) 814
BstSLI GKGCMC 1 cut(s) 529
BstUI CGCG 1 cut(s) 768
BstV1I GCAGC 4 cut(s) 127, 184, 464, 1186
BstV2I GAAGAC 2 cut(s) 722, 748
BstX2I RGATCY 2 cut(s) 215, 728
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 2 cut(s) 215, 728
BsuRI GGCC 1 cut(s) 1276
BtgI CCRYGG 2 cut(s) 1468, 1494
BtsCI GGATG 3 cut(s) 417, 1096, 1504
BtsIMutI CAGTG 1 cut(s) 1245
Cfr13I GGNCC 4 cut(s) 302, 436, 518, 1274
CseI GACGC 1 cut(s) 93
Csp6I GTAC 4 cut(s) 366, 664, 799, 827
CviAII CATG 7 cut(s) 312, 456, 645, 911, 1000, 1469, 1495
CviQI GTAC 4 cut(s) 366, 664, 799, 827
DdeI CTNAG 8 cut(s) 35, 219, 249, 879, 1056, 1193, 1203, 1419
DpnI GATC 4 cut(s) 217, 657, 730, 870
DpnII GATC 4 cut(s) 215, 655, 728, 868
DraI TTTAAA 1 cut(s) 1021
DrdI GACNNNNNNGTC 1 cut(s) 154
DseDI GACNNNNNNGTC 1 cut(s) 154
Eam1104I CTCTTC 2 cut(s) 92, 857
EarI CTCTTC 2 cut(s) 92, 857
Ecl136II GAGCTC 1 cut(s) 1186
Eco130I CCWWGG 4 cut(s) 298, 976, 1468, 1494
Eco24I GRGCYC 1 cut(s) 1188
Eco47I GGWCC 3 cut(s) 302, 436, 518
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 2 cut(s) 735, 1252
EcoICRI GAGCTC 1 cut(s) 1186
EcoNI CCTNNNNNAGG 1 cut(s) 681
EcoO109I RGGNCCY 2 cut(s) 302, 1274
EcoRI GAATTC 1 cut(s) 1067
EcoRII CCWGG 4 cut(s) 520, 724, 731, 1392
EcoT14I CCWWGG 4 cut(s) 298, 976, 1468, 1494
EcoT38I GRGCYC 1 cut(s) 1188
ErhI CCWWGG 4 cut(s) 298, 976, 1468, 1494
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 7 cut(s) 315, 459, 648, 914, 1003, 1472, 1498
FatI CATG 7 cut(s) 311, 455, 644, 910, 999, 1468, 1494
FauI CCCGC 2 cut(s) 761, 1256
FauNDI CATATG 1 cut(s) 823
FbaI TGATCA 1 cut(s) 655
Fnu4HI GCNGC 4 cut(s) 141, 198, 453, 1200
FokI GGATG 2 cut(s) 404, 1103
FriOI GRGCYC 1 cut(s) 1188
Fsp4HI GCNGC 4 cut(s) 141, 198, 453, 1200
FspBI CTAG 3 cut(s) 363, 1298, 1326
GluI GCNGC 4 cut(s) 141, 198, 453, 1200
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 1 cut(s) 1276
HapII CCGG 1 cut(s) 88
HgaI GACGC 1 cut(s) 93
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 7 cut(s) 315, 459, 648, 914, 1003, 1472, 1498
HindIII AAGCTT 1 cut(s) 1211
HinfI GANTC 8 cut(s) 78, 262, 320, 338, 344, 1003, 1174, 1340
HpaII CCGG 1 cut(s) 88
HphI GGTGA 3 cut(s) 696, 1004, 1183
Hpy166II GTNNAC 5 cut(s) 368, 527, 704, 1116, 1465
Hpy188III TCNNGA 5 cut(s) 187, 887, 1064, 1075, 1178
Hpy8I GTNNAC 5 cut(s) 368, 527, 704, 1116, 1465
Hpy99I CGWCG 2 cut(s) 87, 151
HpyAV CCTTC 6 cut(s) 100, 479, 759, 906, 933, 989
HpyCH4III ACNGT 5 cut(s) 121, 156, 269, 948, 1105
HpyCH4V TGCA 6 cut(s) 175, 200, 210, 452, 527, 1441
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 889
HpyF3I CTNAG 8 cut(s) 35, 219, 249, 879, 1056, 1193, 1203, 1419
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 7 cut(s) 315, 459, 648, 914, 1003, 1472, 1498
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 4 cut(s) 215, 655, 728, 868
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 3 cut(s) 148, 811, 1191
Lsp1109I GCAGC 4 cut(s) 127, 184, 464, 1186
LweI GCATC 3 cut(s) 184, 268, 892
MaeI CTAG 3 cut(s) 363, 1298, 1326
MaeIII GTNAC 2 cut(s) 557, 1489
MalI GATC 4 cut(s) 217, 657, 730, 870
MboI GATC 4 cut(s) 215, 655, 728, 868
MboII GAAGA 8 cut(s) 109, 727, 748, 874, 1219, 1258, 1372, 1421
MfeI CAATTG 2 cut(s) 431, 831
MflI RGATCY 2 cut(s) 215, 728
MhlI GDGCHC 3 cut(s) 43, 529, 1188
MluCI AATT 9 cut(s) 408, 431, 510, 568, 831, 1067, 1155, 1261, 1279
MlyI GAGTC 3 cut(s) 72, 314, 1012
MmeI TCCRAC 4 cut(s) 106, 170, 252, 1213
MroXI GAANNNNTTC 1 cut(s) 779
MseI TTAA 5 cut(s) 939, 1020, 1107, 1218, 1385
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 5 cut(s) 89, 522, 726, 733, 1394
MunI CAATTG 2 cut(s) 431, 831
Mva1269I GAATGC 2 cut(s) 583, 693
MvaI CCWGG 4 cut(s) 522, 726, 733, 1394
MvnI CGCG 1 cut(s) 768
MwoI GCNNNNNNNGC 2 cut(s) 38, 889
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 2 cut(s) 1468, 1494
NdeI CATATG 1 cut(s) 823
NdeII GATC 4 cut(s) 215, 655, 728, 868
NlaIII CATG 7 cut(s) 315, 459, 648, 914, 1003, 1472, 1498
NlaIV GGNNCC 2 cut(s) 730, 1275
NmeAIII GCCGAG 1 cut(s) 82
NspI RCATGY 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 92
PctI GAATGC 2 cut(s) 583, 693
PdmI GAANNNNTTC 1 cut(s) 779
PfeI GAWTC 5 cut(s) 262, 338, 344, 1174, 1340
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 4 cut(s) 142, 199, 454, 1201
PleI GAGTC 3 cut(s) 72, 314, 1011
PpsI GAGTC 3 cut(s) 72, 314, 1011
PpuMI RGGWCCY 1 cut(s) 302
PshBI ATTAAT 1 cut(s) 1218
PsiI TTATAA 3 cut(s) 125, 596, 1227
Psp124BI GAGCTC 1 cut(s) 1188
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 4 cut(s) 520, 724, 731, 1392
PspGI CCWGG 4 cut(s) 520, 724, 731, 1392
PspN4I GGNNCC 2 cut(s) 730, 1275
PspPI GGNCC 4 cut(s) 302, 436, 518, 1274
PspPPI RGGWCCY 1 cut(s) 302
PsuI RGATCY 2 cut(s) 215, 728
RsaI GTAC 4 cut(s) 367, 665, 800, 828
RsaNI GTAC 4 cut(s) 366, 664, 799, 827
SacI GAGCTC 1 cut(s) 1188
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 939, 1020, 1107, 1218, 1385
SatI GCNGC 4 cut(s) 141, 198, 453, 1200
Sau3AI GATC 4 cut(s) 215, 655, 728, 868
Sau96I GGNCC 4 cut(s) 302, 436, 518, 1274
ScaI AGTACT 1 cut(s) 665
SchI GAGTC 3 cut(s) 72, 314, 1012
ScrFI CCNGG 5 cut(s) 89, 522, 726, 733, 1394
SduI GDGCHC 3 cut(s) 43, 529, 1188
SfaNI GCATC 3 cut(s) 184, 268, 892
SfcI CTRYAG 1 cut(s) 814
SinI GGWCC 3 cut(s) 302, 436, 518
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 9 cut(s) 408, 431, 510, 568, 831, 1067, 1155, 1261, 1279
SsiI CCGC 3 cut(s) 588, 768, 1249
SspMI CTAG 3 cut(s) 363, 1298, 1326
SstI GAGCTC 1 cut(s) 1188
StyD4I CCNGG 5 cut(s) 87, 520, 724, 731, 1392
StyI CCWWGG 4 cut(s) 298, 976, 1468, 1494
TaaI ACNGT 5 cut(s) 121, 156, 269, 948, 1105
TaqI TCGA 3 cut(s) 186, 318, 1121
TasI AATT 9 cut(s) 408, 431, 510, 568, 831, 1067, 1155, 1261, 1279
TatI WGTACW 2 cut(s) 365, 663
TfiI GAWTC 5 cut(s) 262, 338, 344, 1174, 1340
Tru1I TTAA 5 cut(s) 939, 1020, 1107, 1218, 1385
Tru9I TTAA 5 cut(s) 939, 1020, 1107, 1218, 1385
TscAI CASTG 1 cut(s) 1245
TseI GCWGC 4 cut(s) 140, 197, 452, 1199
TspDTI ATGAA 6 cut(s) 459, 498, 510, 525, 594, 1060
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 1 cut(s) 1245
VneI GTGCAC 1 cut(s) 525
VpaK11BI GGWCC 3 cut(s) 302, 436, 518
VspI ATTAAT 1 cut(s) 1218
XagI CCTNNNNNAGG 1 cut(s) 681
XapI RAATTY 2 cut(s) 1067, 1155
XceI RCATGY 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 779
XspI CTAG 3 cut(s) 363, 1298, 1326
ZrmI AGTACT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.