Rroxscaffold_5G00374750

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002719
Physical Location & Seq
Reverse (-)
55731496 .. 55733158
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374750.1

Sequence Viewer

Length: 1068 bp
ATGGAATCTGATTCAGATGTTGACTTTGCTAGTGTACATGGAGATGGGTTTCCTCCTATGGTAGTAGGTAATGGAATTGGAAACATCCCAAATAGTCAATTGGTTCAGTATGAAAGTGCAGCATGCATTGTAGATAATGGATGCAAGAGTATGAAGGGTTACCTGAAAATCGATGGGGGAGGTGCTTCTGATCAGAAGTACTTATTCCGTCCTAAAGCAGGACTACGCATTCCTTGTTCACCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTTAAGCTCCGCGGGGAGAACTATTTCAAAGACAAACAGAAGTACCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTATTGATTTGTTTATCTGCCCAAGGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAGAACCACATGACAAAGTTCCTTCCCTCCTTATTGTTAATATACAGTTTCCTACATATCCTATTACAATGTTTCCTAGTGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTCAGTGAAAATTTTGACACAGACATCTCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGGATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATCGGTTCCTTTCAGGGAAAGGCTAAAAATTATGGCTGGATTGGTGAATCCAGACGAGGTCCAACTGAGTTCTGCTGAGAAGAAGCTTTTAAGTGCTTATAATGATAAACCAGTGCTTTCGCGCCCTCAACACAATTTCTTCAGGGGACCTAATTACTTTGAGATTGACCTAGACATACACACATTCAGCTACATCTCCAGGAAAGGGCTTGAATCATCCCGAGACAGATTGAAGAATGGGGTATTTAATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAATTACCGGAGCAAGTCTTGTGTTGCATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTCACTTGGACAAGCACCTGTGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

355

Amino Acids

39.8

Weight (kDa)

5.48

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 90 - 331 1.4e-60 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
AccII CGCG 2 cut(s) 303, 784
AciI CCGC 2 cut(s) 301, 303
AclWI GGATC 2 cut(s) 258, 271
AcsI RAATTY 2 cut(s) 568, 602
AcuI CTGAAG 2 cut(s) 270, 787
AcyI GRCGYC 1 cut(s) 1061
AfaI GTAC 4 cut(s) 36, 200, 335, 363
AfiI CCNNNNNNNGG 3 cut(s) 218, 260, 867
AgeI ACCGGT 1 cut(s) 241
AgsI TTSAA 5 cut(s) 319, 599, 875, 895, 982
AjnI CCWGG 4 cut(s) 259, 266, 860, 927
AluBI AGCT 5 cut(s) 298, 354, 427, 748, 852
AluI AGCT 5 cut(s) 298, 354, 427, 748, 852
Alw26I GTCTC 1 cut(s) 879
AlwI GGATC 2 cut(s) 258, 271
Ama87I CYCGRG 1 cut(s) 882
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 2 cut(s) 568, 602
AsiGI ACCGGT 1 cut(s) 241
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 2 cut(s) 786, 978
AspS9I GGNCC 2 cut(s) 721, 809
AsuHPI GGTGA 3 cut(s) 231, 539, 718
AvaI CYCGRG 1 cut(s) 882
AvaII GGWCC 2 cut(s) 721, 809
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BamHI GGATCC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 131
BccI CCATC 3 cut(s) 38, 167, 518
BciT130I CCWGG 4 cut(s) 261, 268, 862, 929
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 879
BfaI CTAG 3 cut(s) 30, 513, 833
BfmI CTRYAG 2 cut(s) 349, 435
BisI GCNGC 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 121
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 4 cut(s) 261, 268, 862, 929
Bme18I GGWCC 2 cut(s) 721, 809
BmeT110I CYCGRG 1 cut(s) 882
BmgT120I GGNCC 2 cut(s) 721, 809
BmiI GGNNCC 3 cut(s) 265, 669, 810
BmrFI CCNGG 4 cut(s) 261, 268, 862, 929
BmsI GCATC 2 cut(s) 131, 427
BpmI CTGGAG 2 cut(s) 243, 844
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 1061
BsaJI CCNNGG 4 cut(s) 301, 392, 1003, 1029
BsaWI WCCGGW 2 cut(s) 241, 952
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 260, 867
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 1 cut(s) 773
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 4 cut(s) 261, 268, 862, 929
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 4 cut(s) 301, 392, 1003, 1029
BseGI GGATG 5 cut(s) 84, 146, 631, 878, 1039
BseLI CCNNNNNNNGG 3 cut(s) 218, 260, 867
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 4 cut(s) 428, 605, 719, 729
BseNI ACTGG 1 cut(s) 773
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 1 cut(s) 131
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 2 cut(s) 303, 784
BshTI ACCGGT 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 882
BsiSI CCGG 2 cut(s) 242, 953
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 3 cut(s) 218, 260, 867
BsmAI GTCTC 1 cut(s) 879
BsmFI GGGAC 1 cut(s) 822
BsmI GAATGC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 882
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 3 cut(s) 190, 263, 403
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 1003, 1029
BspACI CCGC 2 cut(s) 301, 303
BspCNI CTCAG 4 cut(s) 427, 604, 720, 730
BspDI ATCGAT 1 cut(s) 171
BspFNI CGCG 2 cut(s) 303, 784
BspLI GGNNCC 3 cut(s) 265, 669, 810
BspPI GGATC 2 cut(s) 258, 271
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 241
BsrGI TGTACA 1 cut(s) 34
BsrI ACTGG 1 cut(s) 773
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 4 cut(s) 301, 392, 1003, 1029
BssMI GATC 3 cut(s) 190, 263, 403
BssNI GRCGYC 1 cut(s) 1061
BssT1I CCWWGG 3 cut(s) 392, 1003, 1029
Bst2UI CCWGG 4 cut(s) 261, 268, 862, 929
Bst4CI ACNGT 1 cut(s) 483
Bst6I CTCTTC 1 cut(s) 392
BstACI GRCGYC 1 cut(s) 1061
BstAUI TGTACA 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 124, 974
BstDEI CTNAG 4 cut(s) 414, 591, 728, 738
BstDSI CCRYGG 3 cut(s) 301, 1003, 1029
BstEII GGTNACC 1 cut(s) 158
BstENI CCTNNNNNAGG 1 cut(s) 216
BstF5I GGATG 5 cut(s) 84, 146, 631, 878, 1039
BstFNI CGCG 2 cut(s) 303, 784
BstHHI GCGC 2 cut(s) 786, 978
BstKTI GATC 3 cut(s) 193, 266, 406
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 3 cut(s) 190, 263, 403
BstMWI GCNNNNNNNGC 2 cut(s) 424, 1058
BstNI CCWGG 4 cut(s) 261, 268, 862, 929
BstNSI RCATGY 2 cut(s) 126, 976
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 4 cut(s) 259, 266, 860, 927
BstSFI CTRYAG 2 cut(s) 349, 435
BstUI CGCG 2 cut(s) 303, 784
BstV1I GCAGC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 1 cut(s) 263
Bsu15I ATCGAT 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 3 cut(s) 301, 1003, 1029
BtsCI GGATG 5 cut(s) 84, 146, 631, 878, 1039
BtsIMutI CAGTG 2 cut(s) 568, 780
Cac8I GCNNGC 2 cut(s) 124, 974
CfoI GCGC 2 cut(s) 786, 978
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 2 cut(s) 721, 809
Cfr42I CCGCGG 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 1 cut(s) 1050
Csp6I GTAC 4 cut(s) 35, 199, 334, 362
CspAI ACCGGT 1 cut(s) 241
CviAII CATG 7 cut(s) 38, 123, 446, 535, 973, 1004, 1030
CviJI RGCY 9 cut(s) 253, 298, 354, 427, 685, 698, 748, 852, 871
CviKI_1 RGCY 9 cut(s) 253, 298, 354, 427, 685, 698, 748, 852, 871
CviQI GTAC 4 cut(s) 35, 199, 334, 362
DdeI CTNAG 4 cut(s) 414, 591, 728, 738
DpnI GATC 3 cut(s) 192, 265, 405
DpnII GATC 3 cut(s) 190, 263, 403
DraI TTTAAA 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco130I CCWWGG 3 cut(s) 392, 1003, 1029
Eco47I GGWCC 2 cut(s) 721, 809
Eco57I CTGAAG 2 cut(s) 270, 787
Eco88I CYCGRG 1 cut(s) 882
Eco91I GGTNACC 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 4 cut(s) 259, 266, 860, 927
EcoT14I CCWWGG 3 cut(s) 392, 1003, 1029
EcoT22I ATGCAT 1 cut(s) 128
ErhI CCWWGG 3 cut(s) 392, 1003, 1029
FaeI CATG 7 cut(s) 41, 126, 449, 538, 976, 1007, 1033
FaqI GGGAC 1 cut(s) 822
FatI CATG 7 cut(s) 37, 122, 445, 534, 972, 1003, 1029
FauI CCCGC 1 cut(s) 296
FauNDI CATATG 1 cut(s) 358
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 5 cut(s) 71, 153, 638, 865, 1046
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 3 cut(s) 30, 513, 833
GlaI GCGC 2 cut(s) 785, 977
GluI GCNGC 1 cut(s) 120
GsuI CTGGAG 2 cut(s) 243, 844
HapII CCGG 2 cut(s) 242, 953
HgaI GACGC 1 cut(s) 1050
HhaI GCGC 2 cut(s) 786, 978
Hin1I GRCGYC 1 cut(s) 1061
Hin1II CATG 7 cut(s) 41, 126, 449, 538, 976, 1007, 1033
Hin6I GCGC 2 cut(s) 784, 976
HinP1I GCGC 2 cut(s) 784, 976
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 7 cut(s) 5, 11, 538, 659, 709, 875, 1037
HpaII CCGG 2 cut(s) 242, 953
HphI GGTGA 3 cut(s) 231, 539, 718
Hpy166II GTNNAC 5 cut(s) 22, 35, 239, 651, 1000
Hpy188I TCNGA 5 cut(s) 10, 16, 190, 195, 274
Hpy188III TCNNGA 6 cut(s) 422, 599, 610, 713, 882, 1065
Hpy8I GTNNAC 5 cut(s) 22, 35, 239, 651, 1000
HpyAV CCTTC 5 cut(s) 148, 294, 441, 468, 524
HpyCH4III ACNGT 1 cut(s) 483
HpyCH4V TGCA 4 cut(s) 119, 126, 144, 972
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 1058
HpyF3I CTNAG 4 cut(s) 414, 591, 728, 738
Hsp92I GRCGYC 1 cut(s) 1061
Hsp92II CATG 7 cut(s) 41, 126, 449, 538, 976, 1007, 1033
HspAI GCGC 2 cut(s) 784, 976
Ksp22I TGATCA 1 cut(s) 190
KspI CCGCGG 1 cut(s) 304
Kzo9I GATC 3 cut(s) 190, 263, 403
LmnI GCTCC 3 cut(s) 303, 346, 955
Lsp1109I GCAGC 1 cut(s) 131
LweI GCATC 2 cut(s) 131, 427
MaeI CTAG 3 cut(s) 30, 513, 833
MaeIII GTNAC 2 cut(s) 158, 1024
MalI GATC 3 cut(s) 192, 265, 405
MboI GATC 3 cut(s) 190, 263, 403
MboII GAAGA 5 cut(s) 409, 754, 793, 907, 956
MfeI CAATTG 2 cut(s) 98, 366
MflI RGATCY 1 cut(s) 263
MlyI GAGTC 3 cut(s) 547, 668, 1031
MmeI TCCRAC 1 cut(s) 748
MnlI CCTC 7 cut(s) 63, 173, 473, 600, 616, 712, 798
Mph1103I ATGCAT 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 7 cut(s) 294, 474, 555, 642, 752, 909, 920
MslI CAYNNNNRTG 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 2 cut(s) 242, 953
MspR9I CCNGG 4 cut(s) 261, 268, 862, 929
MunI CAATTG 2 cut(s) 98, 366
Mva1269I GAATGC 1 cut(s) 228
MvaI CCWGG 4 cut(s) 261, 268, 862, 929
MvnI CGCG 2 cut(s) 303, 784
MwoI GCNNNNNNNGC 2 cut(s) 424, 1058
NcoI CCATGG 2 cut(s) 1003, 1029
NdeI CATATG 1 cut(s) 358
NdeII GATC 3 cut(s) 190, 263, 403
NlaIII CATG 7 cut(s) 41, 126, 449, 538, 976, 1007, 1033
NlaIV GGNNCC 3 cut(s) 265, 669, 810
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 2 cut(s) 126, 976
PaeI GCATGC 2 cut(s) 126, 976
PctI GAATGC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 4 cut(s) 5, 11, 709, 875
PflFI GACNNNGTC 1 cut(s) 719
PfoI TCCNGGA 2 cut(s) 259, 860
PinAI ACCGGT 1 cut(s) 241
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 3 cut(s) 546, 667, 1031
PpsI GAGTC 3 cut(s) 546, 667, 1031
PpuMI RGGWCCY 1 cut(s) 809
PsiI TTATAA 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 4 cut(s) 259, 266, 860, 927
PspEI GGTNACC 1 cut(s) 158
PspGI CCWGG 4 cut(s) 259, 266, 860, 927
PspN4I GGNNCC 3 cut(s) 265, 669, 810
PspPI GGNCC 2 cut(s) 721, 809
PspPPI RGGWCCY 1 cut(s) 809
PsuI RGATCY 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 4 cut(s) 36, 200, 335, 363
RsaNI GTAC 4 cut(s) 35, 199, 334, 362
RseI CAYNNNNRTG 1 cut(s) 42
SacII CCGCGG 1 cut(s) 304
SaqAI TTAA 7 cut(s) 294, 474, 555, 642, 752, 909, 920
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 3 cut(s) 190, 263, 403
Sau96I GGNCC 2 cut(s) 721, 809
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 3 cut(s) 547, 668, 1031
ScrFI CCNGG 4 cut(s) 261, 268, 862, 929
SfaNI GCATC 2 cut(s) 131, 427
SfcI CTRYAG 2 cut(s) 349, 435
Sfr303I CCGCGG 1 cut(s) 304
SgrBI CCGCGG 1 cut(s) 304
SinI GGWCC 2 cut(s) 721, 809
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SphI GCATGC 2 cut(s) 126, 976
SsiI CCGC 2 cut(s) 301, 303
SspMI CTAG 3 cut(s) 30, 513, 833
StyD4I CCNGG 4 cut(s) 259, 266, 860, 927
StyI CCWWGG 3 cut(s) 392, 1003, 1029
TaaI ACNGT 1 cut(s) 483
TaqI TCGA 1 cut(s) 171
TatI WGTACW 2 cut(s) 34, 198
TfiI GAWTC 4 cut(s) 5, 11, 709, 875
Tru1I TTAA 7 cut(s) 294, 474, 555, 642, 752, 909, 920
Tru9I TTAA 7 cut(s) 294, 474, 555, 642, 752, 909, 920
TscAI CASTG 2 cut(s) 568, 780
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 3 cut(s) 126, 167, 595
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 2 cut(s) 568, 780
Tth111I GACNNNGTC 1 cut(s) 719
VpaK11BI GGWCC 2 cut(s) 721, 809
XagI CCTNNNNNAGG 1 cut(s) 216
XapI RAATTY 2 cut(s) 568, 602
XceI RCATGY 2 cut(s) 126, 976
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 3 cut(s) 30, 513, 833
ZrmI AGTACT 1 cut(s) 200
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.