RchiOBHm_Chr4g0433211

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002719
Physical Location & Seq
Reverse (-)
57381107 .. 57382354
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40173

Sequence Viewer

Length: 897 bp
ATGTACTCAAGTGCTTCTGATCAGAAGTACTTATTCCGTCCTAAAGCAGGACTACGCATTCCTTGTTCAGCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTTAAGCTCCGCGGGGTGAACTATTTCAAAGACAAACAGAAGTACCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTATTGATTTGTTTATCTGCCCAAGGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAGAACCACATGACAAAGTTCCTTCCCTCCTTATTGTTAATATACAGTTTCCTACATATCCTATTACAATGTTCCCTAGTGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTCAGTGAAAATTTTGACAAAGACATCTCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGGATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCGTCGGTTCCTTTCAGGGAAAGGCTAAAAATTATGGCTGGATTGGTTAATCCAGACGAGGTCCAACTGAGTTCTGCTGAGAAGAAGCTTTTAAGTGCTTATAATGATAAACCAGTGCTTTCGCGCCCTCAACACAATTTCTTCAGGGGACCTAATTATTTTGAGATTGACCTAGACATACACACGTTCAGCTACATCTCCAGGAAAGGGCTTGAATCATCCCGAGACAGATTGAAGAATGGGGTATTTAATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAATTACCGGAGCAAGTCTTGTGTTGTATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTCACTTGGACCAGCACCTGTGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

298

Amino Acids

33.94

Weight (kDa)

6.61

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 33 - 274 3.1e-60 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AccII CGCG 2 cut(s) 132, 613
AciI CCGC 3 cut(s) 71, 130, 132
AclWI GGATC 2 cut(s) 87, 100
AcsI RAATTY 2 cut(s) 397, 431
AcuI CTGAAG 2 cut(s) 99, 616
AcyI GRCGYC 1 cut(s) 890
AfaI GTAC 4 cut(s) 5, 29, 164, 192
AfiI CCNNNNNNNGG 3 cut(s) 47, 89, 696
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 5 cut(s) 148, 428, 704, 724, 811
AjnI CCWGG 4 cut(s) 88, 95, 689, 756
AluBI AGCT 5 cut(s) 127, 183, 256, 577, 681
AluI AGCT 5 cut(s) 127, 183, 256, 577, 681
Alw26I GTCTC 1 cut(s) 708
AlwI GGATC 2 cut(s) 87, 100
AlwNI CAGNNNCTG 1 cut(s) 884
Ama87I CYCGRG 1 cut(s) 711
ApoI RAATTY 2 cut(s) 397, 431
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 2 cut(s) 615, 807
AspS9I GGNCC 3 cut(s) 550, 638, 875
AsuHPI GGTGA 2 cut(s) 148, 368
AvaI CYCGRG 1 cut(s) 711
AvaII GGWCC 3 cut(s) 550, 638, 875
BaeI ACNNNNGTAYC 2 cut(s) 146, 179
BamHI GGATCC 1 cut(s) 92
BccI CCATC 1 cut(s) 347
BciT130I CCWGG 4 cut(s) 90, 97, 691, 758
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 708
BfaI CTAG 2 cut(s) 342, 662
BfmI CTRYAG 2 cut(s) 178, 264
BlpI GCTNAGC 1 cut(s) 243
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 4 cut(s) 90, 97, 691, 758
Bme18I GGWCC 3 cut(s) 550, 638, 875
BmeT110I CYCGRG 1 cut(s) 711
BmgT120I GGNCC 3 cut(s) 550, 638, 875
BmiI GGNNCC 3 cut(s) 94, 498, 639
BmrFI CCNGG 4 cut(s) 90, 97, 691, 758
BmsI GCATC 1 cut(s) 256
BpmI CTGGAG 2 cut(s) 72, 673
Bpu1102I GCTNAGC 1 cut(s) 243
BpuEI CTTGAG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 890
BsaJI CCNNGG 4 cut(s) 130, 221, 832, 858
BsaWI WCCGGW 1 cut(s) 781
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 89, 696
Bse1I ACTGG 1 cut(s) 602
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 4 cut(s) 90, 97, 691, 758
BseDI CCNNGG 4 cut(s) 130, 221, 832, 858
BseGI GGATG 3 cut(s) 460, 707, 868
BseLI CCNNNNNNNGG 3 cut(s) 47, 89, 696
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 4 cut(s) 257, 434, 548, 558
BseNI ACTGG 1 cut(s) 602
BseRI GAGGAG 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 132, 613
BsiHKCI CYCGRG 1 cut(s) 711
BsiSI CCGG 1 cut(s) 782
BslFI GGGAC 1 cut(s) 651
BslI CCNNNNNNNGG 3 cut(s) 47, 89, 696
BsmAI GTCTC 1 cut(s) 708
BsmFI GGGAC 1 cut(s) 651
BsmI GAATGC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 711
Bsp143I GATC 3 cut(s) 19, 92, 232
Bsp1720I GCTNAGC 1 cut(s) 243
Bsp19I CCATGG 2 cut(s) 832, 858
BspACI CCGC 3 cut(s) 71, 130, 132
BspCNI CTCAG 4 cut(s) 256, 433, 549, 559
BspFNI CGCG 2 cut(s) 132, 613
BspLI GGNNCC 3 cut(s) 94, 498, 639
BspPI GGATC 2 cut(s) 87, 100
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 602
BssECI CCNNGG 4 cut(s) 130, 221, 832, 858
BssMI GATC 3 cut(s) 19, 92, 232
BssNI GRCGYC 1 cut(s) 890
BssT1I CCWWGG 3 cut(s) 221, 832, 858
Bst2UI CCWGG 4 cut(s) 90, 97, 691, 758
Bst4CI ACNGT 1 cut(s) 312
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 890
BstDEI CTNAG 4 cut(s) 243, 420, 557, 567
BstDSI CCRYGG 3 cut(s) 130, 832, 858
BstENI CCTNNNNNAGG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 460, 707, 868
BstFNI CGCG 2 cut(s) 132, 613
BstHHI GCGC 2 cut(s) 615, 807
BstKTI GATC 3 cut(s) 22, 95, 235
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 3 cut(s) 19, 92, 232
BstMWI GCNNNNNNNGC 2 cut(s) 253, 887
BstNI CCWGG 4 cut(s) 90, 97, 691, 758
BstSCI CCNGG 4 cut(s) 88, 95, 689, 756
BstSFI CTRYAG 2 cut(s) 178, 264
BstUI CGCG 2 cut(s) 132, 613
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 96
BstYI RGATCY 1 cut(s) 92
BtgI CCRYGG 3 cut(s) 130, 832, 858
BtsCI GGATG 3 cut(s) 460, 707, 868
BtsIMutI CAGTG 2 cut(s) 397, 609
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 2 cut(s) 615, 807
Cfr13I GGNCC 3 cut(s) 550, 638, 875
Cfr42I CCGCGG 1 cut(s) 133
CseI GACGC 1 cut(s) 879
Csp6I GTAC 4 cut(s) 4, 28, 163, 191
CviAII CATG 4 cut(s) 275, 364, 833, 859
CviJI RGCY 9 cut(s) 82, 127, 183, 256, 514, 527, 577, 681, 700
CviKI_1 RGCY 9 cut(s) 82, 127, 183, 256, 514, 527, 577, 681, 700
CviQI GTAC 4 cut(s) 4, 28, 163, 191
DdeI CTNAG 4 cut(s) 243, 420, 557, 567
DpnI GATC 3 cut(s) 21, 94, 234
DpnII GATC 3 cut(s) 19, 92, 232
DraI TTTAAA 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 3 cut(s) 221, 832, 858
Eco47I GGWCC 3 cut(s) 550, 638, 875
Eco57I CTGAAG 2 cut(s) 99, 616
Eco88I CYCGRG 1 cut(s) 711
EcoNI CCTNNNNNAGG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 4 cut(s) 88, 95, 689, 756
EcoT14I CCWWGG 3 cut(s) 221, 832, 858
ErhI CCWWGG 3 cut(s) 221, 832, 858
FaeI CATG 4 cut(s) 278, 367, 836, 862
FaqI GGGAC 1 cut(s) 651
FatI CATG 4 cut(s) 274, 363, 832, 858
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 187
FbaI TGATCA 1 cut(s) 19
FokI GGATG 3 cut(s) 467, 694, 875
FspBI CTAG 2 cut(s) 342, 662
GlaI GCGC 2 cut(s) 614, 806
GsuI CTGGAG 2 cut(s) 72, 673
HapII CCGG 1 cut(s) 782
HgaI GACGC 1 cut(s) 879
HhaI GCGC 2 cut(s) 615, 807
Hin1I GRCGYC 1 cut(s) 890
Hin1II CATG 4 cut(s) 278, 367, 836, 862
Hin6I GCGC 2 cut(s) 613, 805
HinP1I GCGC 2 cut(s) 613, 805
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 4 cut(s) 367, 488, 704, 866
HpaII CCGG 1 cut(s) 782
HphI GGTGA 2 cut(s) 148, 368
Hpy166II GTNNAC 3 cut(s) 139, 480, 829
Hpy188I TCNGA 3 cut(s) 19, 24, 103
Hpy188III TCNNGA 6 cut(s) 251, 428, 439, 542, 711, 894
Hpy8I GTNNAC 3 cut(s) 139, 480, 829
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 4 cut(s) 123, 270, 297, 353
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4IV ACGT 1 cut(s) 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 887
HpyF3I CTNAG 4 cut(s) 243, 420, 557, 567
HpySE526I ACGT 1 cut(s) 674
Hsp92I GRCGYC 1 cut(s) 890
Hsp92II CATG 4 cut(s) 278, 367, 836, 862
HspAI GCGC 2 cut(s) 613, 805
Ksp22I TGATCA 1 cut(s) 19
KspI CCGCGG 1 cut(s) 133
Kzo9I GATC 3 cut(s) 19, 92, 232
LmnI GCTCC 3 cut(s) 132, 175, 784
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 342, 662
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 1 cut(s) 853
MalI GATC 3 cut(s) 21, 94, 234
MboI GATC 3 cut(s) 19, 92, 232
MboII GAAGA 5 cut(s) 238, 583, 622, 736, 785
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 1 cut(s) 92
MluCI AATT 8 cut(s) 195, 397, 431, 519, 625, 643, 739, 776
MlyI GAGTC 3 cut(s) 376, 497, 860
MmeI TCCRAC 1 cut(s) 577
MnlI CCTC 5 cut(s) 302, 429, 445, 541, 627
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 8 cut(s) 123, 303, 384, 471, 537, 581, 738, 749
MspA1I CMGCKG 2 cut(s) 71, 132
MspI CCGG 1 cut(s) 782
MspR9I CCNGG 4 cut(s) 90, 97, 691, 758
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 57
MvaI CCWGG 4 cut(s) 90, 97, 691, 758
MvnI CGCG 2 cut(s) 132, 613
MwoI GCNNNNNNNGC 2 cut(s) 253, 887
NcoI CCATGG 2 cut(s) 832, 858
NdeI CATATG 1 cut(s) 187
NdeII GATC 3 cut(s) 19, 92, 232
NlaIII CATG 4 cut(s) 278, 367, 836, 862
NlaIV GGNNCC 3 cut(s) 94, 498, 639
PctI GAATGC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 704
PflFI GACNNNGTC 1 cut(s) 548
PfoI TCCNGGA 2 cut(s) 88, 689
PleI GAGTC 3 cut(s) 375, 496, 860
PpsI GAGTC 3 cut(s) 375, 496, 860
PpuMI RGGWCCY 1 cut(s) 638
PsiI TTATAA 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 4 cut(s) 88, 95, 689, 756
PspGI CCWGG 4 cut(s) 88, 95, 689, 756
PspN4I GGNNCC 3 cut(s) 94, 498, 639
PspPI GGNCC 3 cut(s) 550, 638, 875
PspPPI RGGWCCY 1 cut(s) 638
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 1 cut(s) 92
PsyI GACNNNGTC 1 cut(s) 548
RsaI GTAC 4 cut(s) 5, 29, 164, 192
RsaNI GTAC 4 cut(s) 4, 28, 163, 191
SacII CCGCGG 1 cut(s) 133
SaqAI TTAA 8 cut(s) 123, 303, 384, 471, 537, 581, 738, 749
Sau3AI GATC 3 cut(s) 19, 92, 232
Sau96I GGNCC 3 cut(s) 550, 638, 875
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 3 cut(s) 376, 497, 860
ScrFI CCNGG 4 cut(s) 90, 97, 691, 758
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 178, 264
Sfr303I CCGCGG 1 cut(s) 133
SgrBI CCGCGG 1 cut(s) 133
SinI GGWCC 3 cut(s) 550, 638, 875
SmlI CTYRAG 2 cut(s) 7, 251
SmoI CTYRAG 2 cut(s) 7, 251
Sse9I AATT 8 cut(s) 195, 397, 431, 519, 625, 643, 739, 776
SsiI CCGC 3 cut(s) 71, 130, 132
SspMI CTAG 2 cut(s) 342, 662
StyD4I CCNGG 4 cut(s) 88, 95, 689, 756
StyI CCWWGG 3 cut(s) 221, 832, 858
TaaI ACNGT 1 cut(s) 312
TaiI ACGT 1 cut(s) 677
TasI AATT 8 cut(s) 195, 397, 431, 519, 625, 643, 739, 776
TatI WGTACW 2 cut(s) 3, 27
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 8 cut(s) 123, 303, 384, 471, 537, 581, 738, 749
Tru9I TTAA 8 cut(s) 123, 303, 384, 471, 537, 581, 738, 749
TscAI CASTG 2 cut(s) 397, 609
TspDTI ATGAA 1 cut(s) 424
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 2 cut(s) 397, 609
Tth111I GACNNNGTC 1 cut(s) 548
VpaK11BI GGWCC 3 cut(s) 550, 638, 875
XagI CCTNNNNNAGG 1 cut(s) 45
XapI RAATTY 2 cut(s) 397, 431
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 2 cut(s) 342, 662
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.