MD13G1067700.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002719
Physical Location & Seq
Reverse (-)
4640599 .. 4646115
5517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1067700.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGGGCGTTTGTTACTCAAGACCACATGAAACTATCAAAGCTCATCAGAAAAAGGTCCACAGATCCGGCAAACGCCGGATTAAGGGGAGGAAGACGAAGAAGGTTGCCTCCGCTGCCATTCCCGATGCGCCTAAAAGACAGTCTGTGAGTGAGTTTGTGCATCTTGACTTCGAGAAAGGTGCTGCAACAACCTGCAAGAGATCTGAGGTTTCTAATGGAACATTTCATCTCACTCAGCTTCAGTGGAACCACAGTCAAATAGATGCAAATGGCGGGAAAGGCCCAGGGGAAGCATGGTTTGACTCACTAAGCATGATGGAATCCGATTCAGAAGACGATTTCTGCAGTGTGCATGGAGATGGGTTTCCTTTAGCAGGGAACATCCCAAATGCTCAACTGGTGCAGTATGAAACCGCATCATGCATTGTCGATAATGGATGTAAGTACGAAGGGTTCTACGAAAGTTACCTAAAAATAGATGGAAATGCACGCCATTCTGTCGAGACAACCCAAGTGAGTTTCAACGACAAGAATCAGAAGAACCCCGCGGCTGTGATTATGCTTTCTTACAAGAGGAAATCCGTTGACGACAGCGAAAGGAAAAGTGCGTCCGATCAGAAGTACTTATTCCGTCCAAGAGCAGGACTTCGAATCCCTTGCTCAACGGAAGAGAAGCCAGCACCGGGATCCTGGTCTCCAGTTCCACCCTCAGTATTCAAGCTCCGCGGCGAGAATTACTTCAAAGATAAACTGAAGTACCCGGCTCCAAACTGCAGCTCGTACGTACCAATTGGTGTCGATTTGTTCATCTGCCCGCGAAAGAGAGATCACATTGCCCAGCATCTTGAGCTTCCTTCTGTAAAACCACATGACAAAGTTCCTTCCCTCCTCATTGTTAACATCCAGTTGCCTACTTATCCTACAACAATGTTCCTCGGCGAATGCGATGGGGAAGGCATGAGTCTTGTGCTATATTTCAAAGTGAATGAAAACTTTGAAAAAGAGATATCACCTCAGTTTCAAGACAGCATCAGGAAATTTGTTGAGGATGAGACTGAGAAGGTGAAGGGGTTTGCAAAGGAGTCTGTGGTTCCTTTCAGGGAAAGGCTAAAGATCATGGCTGGATTGGTCAATCCAGATGACCTCCAGTTGAGTTCTACTGAAAGAAAGCTCATACATGCTTACAATGATAAGCCAGTTCTTTCACGCCCTCAACACAATTTCTTCAGGGGACCTAATTACTTTGAGATTGACCTAGATATACATAGGTTTAGCTACATATCCAGGAAAGGACTTGAATCATTCAGAGAGACTTTGAAGAATGGGGTACTTGATTTGGGATTAACAATCCAGGCACAAAAACAAGAGGAATTGCCAGAGCAAGTCTTGTGCTGCCTGCGCTTGAACAAGATTGAATTTGTGAACCATGGTCAAATTCCGACTCTCATAACTATGGACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

488

Amino Acids

55.17

Weight (kDa)

8.33

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 231 - 472 5.3e-61 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 200
AccII CGCG 3 cut(s) 548, 726, 817
AciI CCGC 8 cut(s) 111, 273, 414, 546, 548, 724, 726, 815
AclWI GGATC 3 cut(s) 57, 681, 694
AcsI RAATTY 3 cut(s) 1037, 1415, 1434
AcuI CTGAAG 3 cut(s) 224, 773, 1210
AfaI GTAC 6 cut(s) 446, 623, 758, 782, 786, 1329
AfiI CCNNNNNNNGG 4 cut(s) 82, 374, 641, 683
AjnI CCWGG 4 cut(s) 283, 689, 1283, 1350
AluBI AGCT 7 cut(s) 41, 238, 721, 777, 850, 1171, 1275
AluI AGCT 7 cut(s) 41, 238, 721, 777, 850, 1171, 1275
Alw26I GTCTC 4 cut(s) 497, 699, 1046, 1304
AlwI GGATC 3 cut(s) 57, 681, 694
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 4 cut(s) 113, 182, 774, 1392
ApoI RAATTY 3 cut(s) 1037, 1415, 1434
Asp700I GAANNNNTTC 1 cut(s) 737
AspLEI GCGC 2 cut(s) 130, 1401
AspS9I GGNCC 3 cut(s) 55, 281, 1232
AsuC2I CCSGG 2 cut(s) 684, 761
AsuHPI GGTGA 2 cut(s) 1002, 1075
AsuII TTCGAA 1 cut(s) 649
AvaII GGWCC 2 cut(s) 55, 1232
BaeI ACNNNNGTAYC 2 cut(s) 740, 773
BamHI GGATCC 1 cut(s) 686
BbsI GAAGAC 2 cut(s) 98, 339
BbvI GCAGC 4 cut(s) 100, 169, 786, 1379
BccI CCATC 4 cut(s) 310, 353, 473, 941
BcgI CGANNNNNNTGC 4 cut(s) 161, 195, 639, 673
BciT130I CCWGG 4 cut(s) 285, 691, 1285, 1352
BcnI CCSGG 2 cut(s) 684, 761
BcoDI GTCTC 4 cut(s) 497, 699, 1046, 1304
BfaI CTAG 1 cut(s) 1256
BfmI CTRYAG 2 cut(s) 343, 772
BfuAI ACCTGC 1 cut(s) 200
BglII AGATCT 1 cut(s) 200
BisI GCNGC 6 cut(s) 114, 183, 549, 727, 775, 1393
BlsI GCNGC 6 cut(s) 115, 184, 550, 728, 776, 1394
BmcAI AGTACT 1 cut(s) 623
Bme1390I CCNGG 6 cut(s) 285, 684, 691, 761, 1285, 1352
Bme18I GGWCC 2 cut(s) 55, 1232
BmgT120I GGNCC 3 cut(s) 55, 281, 1232
BmiI GGNNCC 5 cut(s) 248, 688, 765, 1092, 1233
BmrFI CCNGG 6 cut(s) 285, 684, 691, 761, 1285, 1352
BmsI GCATC 6 cut(s) 115, 169, 253, 425, 850, 1038
BpiI GAAGAC 2 cut(s) 98, 339
BpmI CTGGAG 2 cut(s) 681, 1130
Bpu14I TTCGAA 1 cut(s) 649
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 2 cut(s) 684, 761
BsaAI YACGTR 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 6 cut(s) 283, 284, 546, 724, 934, 1426
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 374, 641, 683
Bse1I ACTGG 5 cut(s) 402, 698, 904, 1147, 1196
Bse3DI GCAATG 1 cut(s) 831
BseBI CCWGG 4 cut(s) 285, 691, 1285, 1352
BseDI CCNNGG 6 cut(s) 283, 284, 546, 724, 934, 1426
BseGI GGATG 4 cut(s) 381, 443, 900, 1054
BseLI CCNNNNNNNGG 4 cut(s) 82, 374, 641, 683
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 5 cut(s) 195, 248, 723, 1028, 1047
BseNI ACTGG 5 cut(s) 402, 698, 904, 1147, 1196
BseRI GAGGAG 1 cut(s) 878
BseXI GCAGC 4 cut(s) 100, 169, 786, 1379
BseYI CCCAGC 1 cut(s) 837
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 3 cut(s) 548, 726, 817
BshFI GGCC 1 cut(s) 282
BsiSI CCGG 4 cut(s) 66, 76, 683, 761
BsiWI CGTACG 1 cut(s) 780
BslFI GGGAC 1 cut(s) 1245
BslI CCNNNNNNNGG 4 cut(s) 82, 374, 641, 683
BsmAI GTCTC 4 cut(s) 497, 699, 1046, 1304
BsmFI GGGAC 1 cut(s) 1245
BsmI GAATGC 1 cut(s) 947
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 699
Bsp119I TTCGAA 1 cut(s) 649
Bsp143I GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
Bsp19I CCATGG 1 cut(s) 1426
BspACI CCGC 8 cut(s) 111, 273, 414, 546, 548, 724, 726, 815
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 5 cut(s) 196, 247, 722, 1027, 1048
BspFNI CGCG 3 cut(s) 548, 726, 817
BspLI GGNNCC 5 cut(s) 248, 688, 765, 1092, 1233
BspMAI CTGCAG 2 cut(s) 347, 776
BspMI ACCTGC 1 cut(s) 200
BspPI GGATC 3 cut(s) 57, 681, 694
BspT104I TTCGAA 1 cut(s) 649
BspTNI GGTCTC 1 cut(s) 699
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 5 cut(s) 402, 698, 904, 1147, 1196
BssECI CCNNGG 6 cut(s) 283, 284, 546, 724, 934, 1426
BssMI GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
BssT1I CCWWGG 1 cut(s) 1426
Bst2UI CCWGG 4 cut(s) 285, 691, 1285, 1352
Bst4CI ACNGT 2 cut(s) 141, 254
Bst6I CTCTTC 1 cut(s) 663
BstBAI YACGTR 1 cut(s) 784
BstBI TTCGAA 1 cut(s) 649
BstC8I GCNNGC 4 cut(s) 490, 678, 815, 1397
BstDEI CTNAG 6 cut(s) 204, 234, 308, 709, 1014, 1056
BstDSI CCRYGG 3 cut(s) 546, 724, 1426
BstENI CCTNNNNNAGG 1 cut(s) 372
BstF5I GGATG 4 cut(s) 381, 443, 900, 1054
BstFNI CGCG 3 cut(s) 548, 726, 817
BstHHI GCGC 2 cut(s) 130, 1401
BstKTI GATC 6 cut(s) 65, 203, 616, 689, 829, 1116
BstMAI GTCTC 4 cut(s) 497, 699, 1046, 1304
BstMBI GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
BstMWI GCNNNNNNNGC 4 cut(s) 113, 279, 847, 1398
BstNI CCWGG 4 cut(s) 285, 691, 1285, 1352
BstNSI RCATGY 1 cut(s) 1181
BstSCI CCNGG 6 cut(s) 283, 682, 689, 759, 1283, 1350
BstSFI CTRYAG 2 cut(s) 343, 772
BstSNI TACGTA 1 cut(s) 784
BstUI CGCG 3 cut(s) 548, 726, 817
BstV1I GCAGC 4 cut(s) 100, 169, 786, 1379
BstV2I GAAGAC 2 cut(s) 98, 339
BstX2I RGATCY 3 cut(s) 62, 200, 686
BstYI RGATCY 3 cut(s) 62, 200, 686
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 3 cut(s) 546, 724, 1426
BtgZI GCGATG 1 cut(s) 960
BtsCI GGATG 4 cut(s) 381, 443, 900, 1054
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 2 cut(s) 248, 352
BveI ACCTGC 1 cut(s) 200
Cac8I GCNNGC 4 cut(s) 490, 678, 815, 1397
CfoI GCGC 2 cut(s) 130, 1401
Cfr13I GGNCC 3 cut(s) 55, 281, 1232
Cfr42I CCGCGG 2 cut(s) 549, 727
CseI GACGC 1 cut(s) 597
Csp6I GTAC 6 cut(s) 445, 622, 757, 781, 785, 1328
CviQI GTAC 6 cut(s) 445, 622, 757, 781, 785, 1328
DdeI CTNAG 6 cut(s) 204, 234, 308, 709, 1014, 1056
DpnI GATC 6 cut(s) 64, 202, 615, 688, 828, 1115
DpnII GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco105I TACGTA 1 cut(s) 784
Eco130I CCWWGG 1 cut(s) 1426
Eco31I GGTCTC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 1008
Eco47I GGWCC 2 cut(s) 55, 1232
Eco57I CTGAAG 3 cut(s) 224, 773, 1210
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 1232
EcoRII CCWGG 4 cut(s) 283, 689, 1283, 1350
EcoRV GATATC 1 cut(s) 1008
EcoT14I CCWWGG 1 cut(s) 1426
EcoT22I ATGCAT 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 1426
FaqI GGGAC 1 cut(s) 1245
FauI CCCGC 3 cut(s) 266, 553, 822
Fnu4HI GCNGC 6 cut(s) 114, 183, 549, 727, 775, 1393
FokI GGATG 4 cut(s) 368, 450, 887, 1061
Fsp4HI GCNGC 6 cut(s) 114, 183, 549, 727, 775, 1393
FspBI CTAG 1 cut(s) 1256
GlaI GCGC 2 cut(s) 129, 1400
GluI GCNGC 6 cut(s) 114, 183, 549, 727, 775, 1393
GsaI CCCAGC 1 cut(s) 841
GsuI CTGGAG 2 cut(s) 681, 1130
HaeIII GGCC 1 cut(s) 282
HapII CCGG 4 cut(s) 66, 76, 683, 761
HgaI GACGC 1 cut(s) 597
HhaI GCGC 2 cut(s) 130, 1401
Hin6I GCGC 2 cut(s) 128, 1399
HinP1I GCGC 2 cut(s) 128, 1399
HincII GTYRAC 2 cut(s) 586, 898
HindII GTYRAC 2 cut(s) 586, 898
HinfI GANTC 9 cut(s) 302, 320, 326, 532, 651, 961, 1082, 1298, 1441
HpaI GTTAAC 1 cut(s) 898
HpaII CCGG 4 cut(s) 66, 76, 683, 761
HphI GGTGA 2 cut(s) 1002, 1075
Hpy166II GTNNAC 4 cut(s) 58, 586, 898, 1423
Hpy188I TCNGA 9 cut(s) 48, 205, 325, 331, 537, 613, 618, 1307, 1440
Hpy188III TCNNGA 9 cut(s) 18, 122, 164, 172, 502, 845, 1022, 1033, 1136
Hpy8I GTNNAC 4 cut(s) 58, 586, 898, 1423
HpyAV CCTTC 7 cut(s) 94, 443, 864, 891, 947, 1054, 1060
HpyCH4III ACNGT 2 cut(s) 141, 254
HpyCH4IV ACGT 1 cut(s) 783
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 279, 847, 1398
HpyF3I CTNAG 6 cut(s) 204, 234, 308, 709, 1014, 1056
HpySE526I ACGT 1 cut(s) 783
HspAI GCGC 2 cut(s) 128, 1399
KspAI GTTAAC 1 cut(s) 898
KspI CCGCGG 2 cut(s) 549, 727
Kzo9I GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
LmnI GCTCC 2 cut(s) 726, 769
Lsp1109I GCAGC 4 cut(s) 100, 169, 786, 1379
LweI GCATC 6 cut(s) 115, 169, 253, 425, 850, 1038
MaeI CTAG 1 cut(s) 1256
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 2 cut(s) 11, 464
MalI GATC 6 cut(s) 64, 202, 615, 688, 828, 1115
MboI GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
MboII GAAGA 7 cut(s) 103, 109, 344, 550, 680, 1216, 1330
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 3 cut(s) 62, 200, 686
MluCI AATT 8 cut(s) 733, 789, 1037, 1219, 1237, 1370, 1415, 1434
MlyI GAGTC 4 cut(s) 296, 970, 1091, 1435
MmeI TCCRAC 1 cut(s) 1463
Mph1103I ATGCAT 1 cut(s) 425
MroXI GAANNNNTTC 1 cut(s) 737
MseI TTAA 4 cut(s) 81, 897, 1343, 1462
MslI CAYNNNNRTG 2 cut(s) 357, 1451
MspA1I CMGCKG 3 cut(s) 113, 548, 726
MspI CCGG 4 cut(s) 66, 76, 683, 761
MspR9I CCNGG 6 cut(s) 285, 684, 691, 761, 1285, 1352
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 947
MvaI CCWGG 4 cut(s) 285, 691, 1285, 1352
MvnI CGCG 3 cut(s) 548, 726, 817
MwoI GCNNNNNNNGC 4 cut(s) 113, 279, 847, 1398
NciI CCSGG 2 cut(s) 684, 761
NcoI CCATGG 1 cut(s) 1426
NdeII GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
NlaIV GGNNCC 5 cut(s) 248, 688, 765, 1092, 1233
NmeAIII GCCGAG 1 cut(s) 915
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 1 cut(s) 1181
NspV TTCGAA 1 cut(s) 649
PasI CCCWGGG 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 942
PctI GAATGC 1 cut(s) 947
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 5 cut(s) 320, 326, 532, 651, 1298
Pfl23II CGTACG 1 cut(s) 780
PfoI TCCNGGA 1 cut(s) 1283
PkrI GCNGC 6 cut(s) 115, 184, 550, 728, 776, 1394
PleI GAGTC 4 cut(s) 296, 969, 1090, 1435
PpsI GAGTC 4 cut(s) 296, 969, 1090, 1435
Ppu21I YACGTR 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 1232
Psp5II RGGWCCY 1 cut(s) 1232
Psp6I CCWGG 4 cut(s) 283, 689, 1283, 1350
PspFI CCCAGC 1 cut(s) 837
PspGI CCWGG 4 cut(s) 283, 689, 1283, 1350
PspLI CGTACG 1 cut(s) 780
PspN4I GGNNCC 5 cut(s) 248, 688, 765, 1092, 1233
PspPI GGNCC 3 cut(s) 55, 281, 1232
PspPPI RGGWCCY 1 cut(s) 1232
PsrI GAACNNNNNNTAC 2 cut(s) 437, 469
PstI CTGCAG 2 cut(s) 347, 776
PsuI RGATCY 3 cut(s) 62, 200, 686
RsaI GTAC 6 cut(s) 446, 623, 758, 782, 786, 1329
RsaNI GTAC 6 cut(s) 445, 622, 757, 781, 785, 1328
RseI CAYNNNNRTG 2 cut(s) 357, 1451
SacII CCGCGG 2 cut(s) 549, 727
SaqAI TTAA 4 cut(s) 81, 897, 1343, 1462
SatI GCNGC 6 cut(s) 114, 183, 549, 727, 775, 1393
Sau3AI GATC 6 cut(s) 62, 200, 613, 686, 826, 1113
Sau96I GGNCC 3 cut(s) 55, 281, 1232
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 4 cut(s) 296, 970, 1091, 1435
ScrFI CCNGG 6 cut(s) 285, 684, 691, 761, 1285, 1352
SfaNI GCATC 6 cut(s) 115, 169, 253, 425, 850, 1038
SfcI CTRYAG 2 cut(s) 343, 772
Sfr303I CCGCGG 2 cut(s) 549, 727
SfuI TTCGAA 1 cut(s) 649
SgrBI CCGCGG 2 cut(s) 549, 727
SinI GGWCC 2 cut(s) 55, 1232
SmiMI CAYNNNNRTG 2 cut(s) 357, 1451
SmlI CTYRAG 2 cut(s) 16, 845
SmoI CTYRAG 2 cut(s) 16, 845
SnaBI TACGTA 1 cut(s) 784
Sse9I AATT 8 cut(s) 733, 789, 1037, 1219, 1237, 1370, 1415, 1434
SsiI CCGC 8 cut(s) 111, 273, 414, 546, 548, 724, 726, 815
SspMI CTAG 1 cut(s) 1256
StyD4I CCNGG 6 cut(s) 283, 682, 689, 759, 1283, 1350
StyI CCWWGG 1 cut(s) 1426
TaaI ACNGT 2 cut(s) 141, 254
TaiI ACGT 1 cut(s) 786
TaqI TCGA 5 cut(s) 171, 429, 501, 649, 798
TasI AATT 8 cut(s) 733, 789, 1037, 1219, 1237, 1370, 1415, 1434
TatI WGTACW 1 cut(s) 621
TauI GCSGC 2 cut(s) 551, 729
TfiI GAWTC 5 cut(s) 320, 326, 532, 651, 1298
Tru1I TTAA 4 cut(s) 81, 897, 1343, 1462
Tru9I TTAA 4 cut(s) 81, 897, 1343, 1462
TscAI CASTG 2 cut(s) 248, 352
TseI GCWGC 4 cut(s) 113, 182, 774, 1392
TspDTI ATGAA 5 cut(s) 42, 215, 423, 796, 1002
TspGWI ACGGA 3 cut(s) 571, 620, 680
TspRI CASTG 2 cut(s) 248, 352
VpaK11BI GGWCC 2 cut(s) 55, 1232
XagI CCTNNNNNAGG 1 cut(s) 372
XapI RAATTY 3 cut(s) 1037, 1415, 1434
XceI RCATGY 1 cut(s) 1181
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 1 cut(s) 1256
ZrmI AGTACT 1 cut(s) 623
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.