MD16G1066600.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002719
Physical Location & Seq
Reverse (-)
4644886 .. 4648466
3581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1066600.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGGCGTCTGTGTCTCGAAACCAAATGAAACTATCAACCCTCATCAGAAAAAGGTCCACAGACTCGGCAAGCGCCGGAGTAAGGGGAAGAAGACGAAGAAGGTTGCTTCCGTCGCCATCCCTGATGCGCCCAAAAGACGGTCTGTGAGTGAGTTTGTGCATCTTGACTTTGAGAAAGGTGCTGCAACCACCTGCAAGAGATCTGAGGTTTCTAATGGAACATATCATCTCACTCAGCTTCAGTGGAACCACAGTCAAATTGATGCACATGGCGGGAAAGGCCCAGGGGAAGCATGGTTTGACTCACTAAGCATTCTGGAATCTGATTCAGAAGATGATTTCTGCAGCGTGCATGGAGATGGGTTTCCTTTAGCAGGGAACATCCCAAATGCTCAATTGCTGCAGTATGAAAGTGCATCATGCATCGTCGATAATGGATGTAAGTACGAAGGGTTCTACGAAAGTTATCTAAAAATAGATGGAAATGCACGCCATTCTGTTGAGAAAACCCAAGTGAGTTTCAACGACAAGAGTCAGAAGAACCCGGCGGTGATCATGCTTTATAAGAGGAAGTCCGTTGACGACAGCGAAAGGAACGGTGCGTCCGATCAGAAGTACTTATTCCGTCCAAGAGCAGGACTTCGAATCCCTTGCTCAACTGAAGAGAAGTCAGCTCCGGGATCCTGGTCTCCAGTTCCACCTTCAGTATTCAAGCTCCGCGGCGAGAATTATTTCAAGGATAAACAGAAGTACCCTGCTGCAAACTGCAGCTCGTACGTACCAATTGGTGTCGATTTGTTCATCTGTCCCCGAAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTATGTAAAACCACATGACAAAGTTCCTTCCCTCCTCATTGTTAACATACAGTTGCCTACTTATCCTACAACAATGTTCCTCGGCGATTCAGATGGGGAAGGCATGAGTCTTGTGCTATATTTCAGAGTGAATGAAAACTTCGAAAAAGAGATATCATCTCAGTTTCAAGACAGCATCATGAAATTCATTGAGGATGAGACCGAGAAGGTGAAGGGGTTTGCAAAGGAGTCTCTGGTTCCTTTCAGGGAAAGGCTAAAGATCATGGCTGGATTGGTCAATCCAGATGATCTCCAGTTGAGTTCTACTGAAAGAAAGCTCATACATGCTTACAACGATAAGCCGGTGCTTTCACGCCCCCAACATAATTTCTTCAAGGGACCTAATTACTTTGAGATTGACCTGGATATACATAGGTTTAGCTACATATCCAGGAAAGGACTTGAATCATTCAGAGACCGTTTGAAGAATGGGGTACTTGATTTGGGATTGACAATCCAAGCACAAAAACAAGAGGAACTGCCAGAGCAAGTCCTGTGCTGTTTACGCTTGAACAAGATTGATTTCGTGAACCACGGTCAAATTCCGACTCTCGTAACTATGGACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

486

Amino Acids

54.92

Weight (kDa)

8.35

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 229 - 470 9.4e-62 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 564
AarI CACCTGC 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 200
AccII CGCG 1 cut(s) 720
AciI CCGC 4 cut(s) 273, 548, 718, 720
AclWI GGATC 2 cut(s) 675, 688
AcsI RAATTY 2 cut(s) 1031, 1428
AcuI CTGAAG 3 cut(s) 224, 681, 687
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 6 cut(s) 446, 617, 752, 776, 780, 1323
AfiI CCNNNNNNNGG 4 cut(s) 82, 138, 374, 635
AgsI TTSAA 8 cut(s) 523, 712, 736, 1016, 1222, 1292, 1312, 1399
AjnI CCWGG 4 cut(s) 283, 683, 1248, 1277
AloI GAACNNNNNNTCC 2 cut(s) 587, 619
AluBI AGCT 7 cut(s) 238, 674, 715, 771, 844, 1165, 1269
AluI AGCT 7 cut(s) 238, 674, 715, 771, 844, 1165, 1269
Alw26I GTCTC 5 cut(s) 19, 693, 1040, 1083, 1296
AlwI GGATC 2 cut(s) 675, 688
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 5 cut(s) 182, 345, 400, 758, 768
ApoI RAATTY 2 cut(s) 1031, 1428
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 2 cut(s) 75, 130
AspS9I GGNCC 3 cut(s) 55, 281, 1226
AsuC2I CCSGG 2 cut(s) 545, 678
AsuHPI GGTGA 2 cut(s) 562, 1069
AsuII TTCGAA 2 cut(s) 643, 990
AvaII GGWCC 2 cut(s) 55, 1226
BaeI ACNNNNGTAYC 2 cut(s) 734, 767
BamHI GGATCC 1 cut(s) 680
BbsI GAAGAC 1 cut(s) 98
BbvI GCAGC 5 cut(s) 169, 357, 387, 745, 780
BccI CCATC 4 cut(s) 125, 353, 473, 935
BcgI CGANNNNNNTGC 2 cut(s) 633, 667
BciT130I CCWGG 4 cut(s) 285, 685, 1250, 1279
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 2 cut(s) 545, 678
BcoDI GTCTC 5 cut(s) 19, 693, 1040, 1083, 1296
BfmI CTRYAG 3 cut(s) 343, 401, 766
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 200
BglII AGATCT 1 cut(s) 200
BisI GCNGC 6 cut(s) 183, 346, 401, 721, 759, 769
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 6 cut(s) 184, 347, 402, 722, 760, 770
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 6 cut(s) 285, 545, 678, 685, 1250, 1279
Bme18I GGWCC 2 cut(s) 55, 1226
BmgT120I GGNCC 3 cut(s) 55, 281, 1226
BmiI GGNNCC 4 cut(s) 248, 682, 1086, 1227
BmrFI CCNGG 6 cut(s) 285, 545, 678, 685, 1250, 1279
BmsI GCATC 7 cut(s) 115, 169, 253, 425, 432, 844, 1032
BoxI GACNNNNGTC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 2 cut(s) 675, 1124
Bpu1102I GCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 2 cut(s) 643, 990
BpuEI CTTGAG 1 cut(s) 860
BpuMI CCSGG 2 cut(s) 545, 678
BsaAI YACGTR 1 cut(s) 778
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 3 cut(s) 693, 1040, 1296
BsaJI CCNNGG 5 cut(s) 283, 284, 718, 928, 1420
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 138, 374, 635
Bse118I RCCGGY 1 cut(s) 1189
Bse1I ACTGG 2 cut(s) 692, 1141
Bse3DI GCAATG 1 cut(s) 825
BseBI CCWGG 4 cut(s) 285, 685, 1250, 1279
BseDI CCNNGG 5 cut(s) 283, 284, 718, 928, 1420
BseGI GGATG 4 cut(s) 117, 381, 443, 1048
BseLI CCNNNNNNNGG 4 cut(s) 82, 138, 374, 635
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 4 cut(s) 195, 248, 845, 1022
BseNI ACTGG 2 cut(s) 692, 1141
BseRI GAGGAG 1 cut(s) 872
BseXI GCAGC 5 cut(s) 169, 357, 387, 745, 780
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 1 cut(s) 282
BsiSI CCGG 4 cut(s) 76, 545, 677, 1190
BsiWI CGTACG 1 cut(s) 774
BslFI GGGAC 2 cut(s) 792, 1239
BslI CCNNNNNNNGG 4 cut(s) 82, 138, 374, 635
BsmAI GTCTC 5 cut(s) 19, 693, 1040, 1083, 1296
BsmFI GGGAC 2 cut(s) 792, 1239
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 3 cut(s) 693, 1040, 1296
Bsp119I TTCGAA 2 cut(s) 643, 990
Bsp143I GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
Bsp1720I GCTNAGC 1 cut(s) 831
BspACI CCGC 4 cut(s) 273, 548, 718, 720
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 4 cut(s) 196, 247, 844, 1021
BspFNI CGCG 1 cut(s) 720
BspHI TCATGA 1 cut(s) 1026
BspLI GGNNCC 4 cut(s) 248, 682, 1086, 1227
BspMAI CTGCAG 3 cut(s) 347, 405, 770
BspMI ACCTGC 1 cut(s) 200
BspPI GGATC 2 cut(s) 675, 688
BspT104I TTCGAA 2 cut(s) 643, 990
BspTNI GGTCTC 3 cut(s) 693, 1040, 1296
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 1189
BsrI ACTGG 2 cut(s) 692, 1141
BssAI RCCGGY 1 cut(s) 1189
BssECI CCNNGG 5 cut(s) 283, 284, 718, 928, 1420
BssMI GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 4 cut(s) 285, 685, 1250, 1279
Bst4CI ACNGT 6 cut(s) 141, 254, 599, 900, 1307, 1424
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 6
BstBAI YACGTR 1 cut(s) 778
BstBI TTCGAA 2 cut(s) 643, 990
BstC8I GCNNGC 3 cut(s) 71, 350, 490
BstDEI CTNAG 5 cut(s) 204, 234, 308, 831, 1008
BstDSI CCRYGG 2 cut(s) 718, 1420
BstENI CCTNNNNNAGG 1 cut(s) 372
BstF5I GGATG 4 cut(s) 117, 381, 443, 1048
BstFNI CGCG 1 cut(s) 720
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 75, 130
BstKTI GATC 7 cut(s) 203, 555, 610, 683, 823, 1110, 1138
BstMAI GTCTC 5 cut(s) 19, 693, 1040, 1083, 1296
BstMBI GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 113, 279, 841, 1392
BstNI CCWGG 4 cut(s) 285, 685, 1250, 1279
BstNSI RCATGY 1 cut(s) 1175
BstPAI GACNNNNGTC 1 cut(s) 531
BstSCI CCNGG 6 cut(s) 283, 543, 676, 683, 1248, 1277
BstSFI CTRYAG 3 cut(s) 343, 401, 766
BstSNI TACGTA 1 cut(s) 778
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 5 cut(s) 169, 357, 387, 745, 780
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 200, 680
BstYI RGATCY 2 cut(s) 200, 680
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 2 cut(s) 718, 1420
BtsCI GGATG 4 cut(s) 117, 381, 443, 1048
BtsIMutI CAGTG 1 cut(s) 248
BveI ACCTGC 1 cut(s) 200
Cac8I GCNNGC 3 cut(s) 71, 350, 490
CciI TCATGA 1 cut(s) 1026
CfoI GCGC 2 cut(s) 75, 130
Cfr10I RCCGGY 1 cut(s) 1189
Cfr13I GGNCC 3 cut(s) 55, 281, 1226
Cfr42I CCGCGG 1 cut(s) 721
CseI GACGC 1 cut(s) 591
Csp6I GTAC 6 cut(s) 445, 616, 751, 775, 779, 1322
CviQI GTAC 6 cut(s) 445, 616, 751, 775, 779, 1322
DdeI CTNAG 5 cut(s) 204, 234, 308, 831, 1008
DpnI GATC 7 cut(s) 202, 554, 609, 682, 822, 1109, 1137
DpnII GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
Eco105I TACGTA 1 cut(s) 778
Eco31I GGTCTC 3 cut(s) 693, 1040, 1296
Eco32I GATATC 1 cut(s) 1002
Eco47I GGWCC 2 cut(s) 55, 1226
Eco57I CTGAAG 3 cut(s) 224, 681, 687
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 1226
EcoRII CCWGG 4 cut(s) 283, 683, 1248, 1277
EcoRV GATATC 1 cut(s) 1002
EcoT22I ATGCAT 1 cut(s) 425
FaqI GGGAC 2 cut(s) 792, 1239
FauI CCCGC 1 cut(s) 266
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 6 cut(s) 183, 346, 401, 721, 759, 769
FokI GGATG 4 cut(s) 104, 368, 450, 1055
Fsp4HI GCNGC 6 cut(s) 183, 346, 401, 721, 759, 769
GlaI GCGC 2 cut(s) 74, 129
GluI GCNGC 6 cut(s) 183, 346, 401, 721, 759, 769
GsuI CTGGAG 2 cut(s) 675, 1124
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 1 cut(s) 282
HapII CCGG 4 cut(s) 76, 545, 677, 1190
HgaI GACGC 1 cut(s) 591
HhaI GCGC 2 cut(s) 75, 130
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 2 cut(s) 73, 128
HinP1I GCGC 2 cut(s) 73, 128
HincII GTYRAC 2 cut(s) 580, 892
HindII GTYRAC 2 cut(s) 580, 892
HpaI GTTAAC 1 cut(s) 892
HpaII CCGG 4 cut(s) 76, 545, 677, 1190
HphI GGTGA 2 cut(s) 562, 1069
Hpy166II GTNNAC 5 cut(s) 58, 580, 892, 1391, 1417
Hpy188III TCNNGA 8 cut(s) 16, 164, 317, 839, 1016, 1027, 1130, 1414
Hpy8I GTNNAC 5 cut(s) 58, 580, 892, 1391, 1417
Hpy99I CGWCG 2 cut(s) 116, 431
HpyAV CCTTC 7 cut(s) 94, 443, 711, 885, 941, 1048, 1054
HpyCH4III ACNGT 6 cut(s) 141, 254, 599, 900, 1307, 1424
HpyCH4IV ACGT 1 cut(s) 777
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 279, 841, 1392
HpyF3I CTNAG 5 cut(s) 204, 234, 308, 831, 1008
HpySE526I ACGT 1 cut(s) 777
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 2 cut(s) 73, 128
Ksp22I TGATCA 1 cut(s) 552
KspAI GTTAAC 1 cut(s) 892
KspI CCGCGG 1 cut(s) 721
Kzo9I GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
LmnI GCTCC 2 cut(s) 679, 720
Lsp1109I GCAGC 5 cut(s) 169, 357, 387, 745, 780
LweI GCATC 7 cut(s) 115, 169, 253, 425, 432, 844, 1032
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 1 cut(s) 1441
MalI GATC 7 cut(s) 202, 554, 609, 682, 822, 1109, 1137
MboI GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
MboII GAAGA 8 cut(s) 100, 103, 109, 344, 550, 674, 1210, 1324
MfeI CAATTG 2 cut(s) 395, 783
MflI RGATCY 2 cut(s) 200, 680
MluCI AATT 8 cut(s) 258, 395, 727, 783, 1031, 1213, 1231, 1428
MlyI GAGTC 6 cut(s) 57, 296, 541, 964, 1085, 1429
MmeI TCCRAC 1 cut(s) 1457
MnlI CCTC 8 cut(s) 51, 199, 561, 890, 893, 938, 1033, 1354
Mph1103I ATGCAT 1 cut(s) 425
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 2 cut(s) 891, 1456
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 720
MspI CCGG 4 cut(s) 76, 545, 677, 1190
MspR9I CCNGG 6 cut(s) 285, 545, 678, 685, 1250, 1279
MunI CAATTG 2 cut(s) 395, 783
Mva1269I GAATGC 1 cut(s) 312
MvaI CCWGG 4 cut(s) 285, 685, 1250, 1279
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 4 cut(s) 113, 279, 841, 1392
NciI CCSGG 2 cut(s) 545, 678
NdeII GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
NlaIV GGNNCC 4 cut(s) 248, 682, 1086, 1227
NmeAIII GCCGAG 2 cut(s) 45, 909
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 1 cut(s) 1175
NspV TTCGAA 2 cut(s) 643, 990
PagI TCATGA 1 cut(s) 1026
PaqCI CACCTGC 1 cut(s) 200
PasI CCCWGGG 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 603
PctI GAATGC 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 5 cut(s) 320, 326, 645, 935, 1292
Pfl23II CGTACG 1 cut(s) 774
PfoI TCCNGGA 2 cut(s) 676, 1277
PkrI GCNGC 6 cut(s) 184, 347, 402, 722, 760, 770
PleI GAGTC 6 cut(s) 57, 296, 540, 963, 1084, 1429
PpsI GAGTC 6 cut(s) 57, 296, 540, 963, 1084, 1429
Ppu21I YACGTR 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 1226
PshAI GACNNNNGTC 1 cut(s) 531
PsiI TTATAA 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 1226
Psp6I CCWGG 4 cut(s) 283, 683, 1248, 1277
PspGI CCWGG 4 cut(s) 283, 683, 1248, 1277
PspLI CGTACG 1 cut(s) 774
PspN4I GGNNCC 4 cut(s) 248, 682, 1086, 1227
PspPI GGNCC 3 cut(s) 55, 281, 1226
PspPPI RGGWCCY 1 cut(s) 1226
PsrI GAACNNNNNNTAC 2 cut(s) 437, 469
PstI CTGCAG 3 cut(s) 347, 405, 770
PsuI RGATCY 2 cut(s) 200, 680
RsaI GTAC 6 cut(s) 446, 617, 752, 776, 780, 1323
RsaNI GTAC 6 cut(s) 445, 616, 751, 775, 779, 1322
RseI CAYNNNNRTG 1 cut(s) 357
SacII CCGCGG 1 cut(s) 721
SaqAI TTAA 2 cut(s) 891, 1456
SatI GCNGC 6 cut(s) 183, 346, 401, 721, 759, 769
Sau3AI GATC 7 cut(s) 200, 552, 607, 680, 820, 1107, 1135
Sau96I GGNCC 3 cut(s) 55, 281, 1226
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 6 cut(s) 57, 296, 541, 964, 1085, 1429
ScrFI CCNGG 6 cut(s) 285, 545, 678, 685, 1250, 1279
SfaNI GCATC 7 cut(s) 115, 169, 253, 425, 432, 844, 1032
SfcI CTRYAG 3 cut(s) 343, 401, 766
Sfr303I CCGCGG 1 cut(s) 721
SfuI TTCGAA 2 cut(s) 643, 990
SgrBI CCGCGG 1 cut(s) 721
SinI GGWCC 2 cut(s) 55, 1226
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
SnaBI TACGTA 1 cut(s) 778
Sse9I AATT 8 cut(s) 258, 395, 727, 783, 1031, 1213, 1231, 1428
SsiI CCGC 4 cut(s) 273, 548, 718, 720
StyD4I CCNGG 6 cut(s) 283, 543, 676, 683, 1248, 1277
TaaI ACNGT 6 cut(s) 141, 254, 599, 900, 1307, 1424
TaiI ACGT 1 cut(s) 780
TaqI TCGA 5 cut(s) 17, 429, 643, 792, 990
TaqII GACCGA 1 cut(s) 1064
TasI AATT 8 cut(s) 258, 395, 727, 783, 1031, 1213, 1231, 1428
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 5 cut(s) 320, 326, 645, 935, 1292
Tru1I TTAA 2 cut(s) 891, 1456
Tru9I TTAA 2 cut(s) 891, 1456
TscAI CASTG 1 cut(s) 248
TseI GCWGC 5 cut(s) 182, 345, 400, 758, 768
TspDTI ATGAA 6 cut(s) 42, 423, 790, 996, 1024, 1043
TspGWI ACGGA 3 cut(s) 100, 565, 614
TspRI CASTG 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 55, 1226
XagI CCTNNNNNAGG 1 cut(s) 372
XapI RAATTY 2 cut(s) 1031, 1428
XceI RCATGY 1 cut(s) 1175
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 731
ZrmI AGTACT 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.