Rw0G007430

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0002719
Physical Location & Seq
Reverse (-)
225776 .. 227841
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007430.1

Sequence Viewer

Length: 1383 bp
ATGGGTGTATGTGTCTCAAAGCCAAGTGAAACTATCAGAGCTCAAAAGAAAAAAGTCGACAGAGTCGGCAAAGGGCGGCGCCGGGGGAGGAAGAGCAAGAAGGTAGCCACCGCCGTTATAAACTCAGAGATTCCTGCTCCGACGAGACAGTCTGTTAGTGAGTTTGTGCGTCTTGACTTTGAGAAAGGTGCTGCAACCACTTGCAGAAGATCTGAGGTTTCCAATGGCACATTTCATCTAACTCAACTTCAATGGAACCACAGTCAGATTGATGGGAATGGTGGGAAATGCCAAGGACCTGAAACCTGGTTTGACTCCCATAGTATAATGGAATCTGATTCAGATGATGACTTTGCTAGTGTACATGGAGTAGGTAATGGAATTGGAAACATCCCAAATAGTCAATTAGTCCAGTATGAAAGTGCAGCATGCATTGTAGATAATGGATGCAAGTATGAAGGGTTTTATGAAAGTTATCTGAAAATCGATGGGGCAGGTGCACGTCATTCCCTAGAGAAAACCCAAGTCAGTTACAATGACAAAAATCAGCCCAAGCAATCAGTTATCATGCTTTCCTACAAGAGAAAATCCATCGACAACCCTGAAAGGATTTCATGTGTTTCTGATCAGAAGTACTTATTCCGTCCTAAATCAGGACAACGGATTCCTTGCTCACCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAATCTTTAACTCATATGTACCAATTGGTATTGATATGTTTATCTGCCCAAGGAAGAGGGATCACATTGCTCAGTATCTTGAGCTTCCTTCTGTAAAACCACATGATAAAGTTCCTTCCCTCCTCATTGTTAATATACAGTTGCCTACATATCCAACTACAATGTTCCTTGGTGAAAGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTTAGTGAAAATTTTGACACAGACATATCTCCTCAGTTTCAAGAATTTATCAAGAGATTTGTTGAGGATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATTGGTTCCTTTCAGGGAAAGGCTAAAAATTTTGGCTGGATTGGTGAATCCAGACGAGCTCCAACTGAGTTCTGCGGAGAAGAAGCTGATAAGTGCTTATAATGATAAACCAGTGCTTTCGCGTCCTCAACACAATTTCTTCAGGGGGTTCAGCTACATCTCCAGGAAAGGGCTTGAATCATTCAGAGACAGATTGAAGAATGGGGTACTTCATGTGGGATTAACAATCCAGGCACAAAAACAAGAAGAACTACCGGAGCAAGTCTTGTGTTGTATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGAATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

460

Amino Acids

51.82

Weight (kDa)

8.64

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 246 - 445 8e-36 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 119, 1140
AarI CACCTGC 1 cut(s) 485
AasI GACNNNNNNGTC 1 cut(s) 148
Acc36I ACCTGC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 57
AccII CGCG 1 cut(s) 1162
AciI CCGC 3 cut(s) 76, 111, 1115
AclWI GGATC 3 cut(s) 693, 706, 789
AcsI RAATTY 3 cut(s) 946, 980, 1068
AcuI CTGAAG 1 cut(s) 1165
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 4 cut(s) 363, 635, 741, 1248
AfiI CCNNNNNNNGG 3 cut(s) 653, 695, 1209
AgeI ACCGGT 1 cut(s) 676
AgsI TTSAA 6 cut(s) 251, 723, 977, 1217, 1237, 1324
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 5 cut(s) 305, 694, 701, 1202, 1269
AluBI AGCT 5 cut(s) 41, 805, 1099, 1126, 1194
AluI AGCT 5 cut(s) 41, 805, 1099, 1126, 1194
Alw21I GWGCWC 3 cut(s) 43, 502, 1101
Alw26I GTCTC 3 cut(s) 19, 139, 1221
Alw44I GTGCAC 1 cut(s) 498
AlwI GGATC 3 cut(s) 693, 706, 789
ApaLI GTGCAC 1 cut(s) 498
ApeKI GCWGC 2 cut(s) 191, 425
ApoI RAATTY 3 cut(s) 946, 980, 1068
AsiGI ACCGGT 1 cut(s) 676
AspLEI GCGC 2 cut(s) 81, 1320
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 4 cut(s) 666, 905, 917, 1096
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 502
BamHI GGATCC 1 cut(s) 698
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 2 cut(s) 43, 1101
Bbv12I GWGCWC 3 cut(s) 43, 502, 1101
BbvI GCAGC 2 cut(s) 178, 437
BccI CCATC 4 cut(s) 266, 482, 599, 896
BceAI ACGGC 1 cut(s) 98
BciT130I CCWGG 5 cut(s) 307, 696, 703, 1204, 1271
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 3 cut(s) 19, 139, 1221
BfaI CTAG 2 cut(s) 357, 512
BfoI RGCGCY 1 cut(s) 82
BfuAI ACCTGC 1 cut(s) 485
BglII AGATCT 1 cut(s) 209
BisI GCNGC 3 cut(s) 77, 192, 426
BlsI GCNGC 3 cut(s) 78, 193, 427
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 6 cut(s) 83, 307, 696, 703, 1204, 1271
Bme18I GGWCC 1 cut(s) 296
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 4 cut(s) 80, 257, 700, 1047
BmrFI CCNGG 6 cut(s) 83, 307, 696, 703, 1204, 1271
BmsI GCATC 1 cut(s) 437
BpmI CTGGAG 2 cut(s) 678, 1186
BpuEI CTTGAG 1 cut(s) 821
BpuMI CCSGG 1 cut(s) 83
Bsa29I ATCGAT 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 79
BsaJI CCNNGG 5 cut(s) 82, 292, 770, 889, 1345
BsaWI WCCGGW 2 cut(s) 676, 1294
Bsc4I CCNNNNNNNGG 3 cut(s) 653, 695, 1209
Bse118I RCCGGY 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 412, 1151
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 5 cut(s) 307, 696, 703, 1204, 1271
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 5 cut(s) 82, 292, 770, 889, 1345
BseGI GGATG 3 cut(s) 390, 452, 1009
BseLI CCNNNNNNNGG 3 cut(s) 653, 695, 1209
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 5 cut(s) 138, 204, 806, 983, 1097
BseNI ACTGG 2 cut(s) 412, 1151
BseRI GAGGAG 2 cut(s) 833, 957
BseSI GKGCMC 1 cut(s) 502
BseXI GCAGC 2 cut(s) 178, 437
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 1162
BshNI GGYRCC 1 cut(s) 78
BshTI ACCGGT 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 486
BsiHKAI GWGCWC 3 cut(s) 43, 502, 1101
BsiSI CCGG 3 cut(s) 82, 677, 1295
BslI CCNNNNNNNGG 3 cut(s) 653, 695, 1209
BsmAI GTCTC 3 cut(s) 19, 139, 1221
Bsp1286I GDGCHC 3 cut(s) 43, 502, 1101
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 4 cut(s) 209, 625, 698, 781
Bsp19I CCATGG 1 cut(s) 1345
BspACI CCGC 3 cut(s) 76, 111, 1115
BspCNI CTCAG 5 cut(s) 137, 205, 805, 982, 1098
BspDI ATCGAT 1 cut(s) 486
BspFNI CGCG 1 cut(s) 1162
BspLI GGNNCC 4 cut(s) 80, 257, 700, 1047
BspMI ACCTGC 1 cut(s) 485
BspPI GGATC 3 cut(s) 693, 706, 789
BspQI GCTCTTC 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 676
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 2 cut(s) 412, 1151
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 5 cut(s) 82, 292, 770, 889, 1345
BssMI GATC 4 cut(s) 209, 625, 698, 781
BssNI GRCGYC 1 cut(s) 79
BssT1I CCWWGG 4 cut(s) 292, 770, 889, 1345
Bst2UI CCWGG 5 cut(s) 307, 696, 703, 1204, 1271
Bst4CI ACNGT 3 cut(s) 150, 263, 861
Bst6I CTCTTC 2 cut(s) 86, 770
BstACI GRCGYC 1 cut(s) 79
BstAUI TGTACA 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 5 cut(s) 124, 213, 792, 969, 1106
BstDSI CCRYGG 1 cut(s) 1345
BstENI CCTNNNNNAGG 1 cut(s) 651
BstF5I GGATG 3 cut(s) 390, 452, 1009
BstFNI CGCG 1 cut(s) 1162
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 2 cut(s) 81, 1320
BstKTI GATC 4 cut(s) 212, 628, 701, 784
BstMAI GTCTC 3 cut(s) 19, 139, 1221
BstMBI GATC 4 cut(s) 209, 625, 698, 781
BstNI CCWGG 5 cut(s) 307, 696, 703, 1204, 1271
BstNSI RCATGY 1 cut(s) 432
BstSCI CCNGG 6 cut(s) 81, 305, 694, 701, 1202, 1269
BstSLI GKGCMC 1 cut(s) 502
BstUI CGCG 1 cut(s) 1162
BstV1I GCAGC 2 cut(s) 178, 437
BstX2I RGATCY 2 cut(s) 209, 698
BstXI CCANNNNNNTGG 1 cut(s) 702
BstYI RGATCY 2 cut(s) 209, 698
Bsu15I ATCGAT 1 cut(s) 486
BsuTUI ATCGAT 1 cut(s) 486
BtgI CCRYGG 1 cut(s) 1345
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 3 cut(s) 390, 452, 1009
BtsIMutI CAGTG 1 cut(s) 1158
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 1 cut(s) 430
CfoI GCGC 2 cut(s) 81, 1320
Cfr10I RCCGGY 1 cut(s) 676
Cfr13I GGNCC 1 cut(s) 296
ClaI ATCGAT 1 cut(s) 486
CseI GACGC 2 cut(s) 158, 1151
CsiI ACCWGGT 1 cut(s) 305
Csp6I GTAC 4 cut(s) 362, 634, 740, 1247
CspAI ACCGGT 1 cut(s) 676
CviAII CATG 9 cut(s) 365, 429, 568, 615, 824, 913, 1253, 1346, 1372
CviQI GTAC 4 cut(s) 362, 634, 740, 1247
DdeI CTNAG 5 cut(s) 124, 213, 792, 969, 1106
DinI GGCGCC 1 cut(s) 80
DpnI GATC 4 cut(s) 211, 627, 700, 783
DpnII GATC 4 cut(s) 209, 625, 698, 781
DraI TTTAAA 1 cut(s) 934
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 86, 770
EarI CTCTTC 2 cut(s) 86, 770
Ecl136II GAGCTC 2 cut(s) 41, 1099
Eco130I CCWWGG 4 cut(s) 292, 770, 889, 1345
Eco24I GRGCYC 2 cut(s) 43, 1101
Eco47I GGWCC 1 cut(s) 296
Eco53kI GAGCTC 2 cut(s) 41, 1099
Eco57I CTGAAG 1 cut(s) 1165
EcoICRI GAGCTC 2 cut(s) 41, 1099
EcoNI CCTNNNNNAGG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 296
EcoRII CCWGG 5 cut(s) 305, 694, 701, 1202, 1269
EcoT14I CCWWGG 4 cut(s) 292, 770, 889, 1345
EcoT22I ATGCAT 1 cut(s) 434
EcoT38I GRGCYC 2 cut(s) 43, 1101
EgeI GGCGCC 1 cut(s) 80
EheI GGCGCC 1 cut(s) 80
ErhI CCWWGG 4 cut(s) 292, 770, 889, 1345
FaeI CATG 9 cut(s) 368, 432, 571, 618, 827, 916, 1256, 1349, 1375
FatI CATG 9 cut(s) 364, 428, 567, 614, 823, 912, 1252, 1345, 1371
FauNDI CATATG 1 cut(s) 736
FbaI TGATCA 1 cut(s) 625
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 77, 192, 426
FokI GGATG 3 cut(s) 377, 459, 1016
FriOI GRGCYC 2 cut(s) 43, 1101
Fsp4HI GCNGC 3 cut(s) 77, 192, 426
FspBI CTAG 2 cut(s) 357, 512
GlaI GCGC 2 cut(s) 80, 1319
GluI GCNGC 3 cut(s) 77, 192, 426
GsuI CTGGAG 2 cut(s) 678, 1186
HaeII RGCGCY 1 cut(s) 82
HapII CCGG 3 cut(s) 82, 677, 1295
HgaI GACGC 2 cut(s) 158, 1151
HhaI GCGC 2 cut(s) 81, 1320
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 9 cut(s) 368, 432, 571, 618, 827, 916, 1256, 1349, 1375
Hin6I GCGC 2 cut(s) 79, 1318
HinP1I GCGC 2 cut(s) 79, 1318
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HpaII CCGG 3 cut(s) 82, 677, 1295
HphI GGTGA 4 cut(s) 666, 905, 917, 1096
Hpy166II GTNNAC 5 cut(s) 58, 362, 500, 1029, 1342
Hpy188III TCNNGA 6 cut(s) 173, 654, 800, 977, 988, 1091
Hpy8I GTNNAC 5 cut(s) 58, 362, 500, 1029, 1342
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 6 cut(s) 94, 452, 729, 819, 846, 902
HpyCH4III ACNGT 3 cut(s) 150, 263, 861
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 6 cut(s) 194, 204, 425, 432, 450, 500
HpyF3I CTNAG 5 cut(s) 124, 213, 792, 969, 1106
HpySE526I ACGT 1 cut(s) 502
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 9 cut(s) 368, 432, 571, 618, 827, 916, 1256, 1349, 1375
HspAI GCGC 2 cut(s) 79, 1318
KasI GGCGCC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 4 cut(s) 209, 625, 698, 781
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 3 cut(s) 142, 1104, 1297
Lsp1109I GCAGC 2 cut(s) 178, 437
LweI GCATC 1 cut(s) 437
MabI ACCWGGT 1 cut(s) 305
MaeI CTAG 2 cut(s) 357, 512
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 2 cut(s) 530, 1366
MalI GATC 4 cut(s) 211, 627, 700, 783
MboI GATC 4 cut(s) 209, 625, 698, 781
MboII GAAGA 7 cut(s) 103, 219, 787, 1132, 1171, 1249, 1298
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 2 cut(s) 209, 698
MhlI GDGCHC 3 cut(s) 43, 502, 1101
MluCI AATT 7 cut(s) 381, 404, 744, 946, 980, 1068, 1174
Mly113I GGCGCC 1 cut(s) 79
MlyI GAGTC 4 cut(s) 72, 308, 925, 1046
MmeI TCCRAC 3 cut(s) 164, 899, 1126
MnlI CCTC 8 cut(s) 81, 208, 771, 851, 854, 978, 994, 1176
Mph1103I ATGCAT 1 cut(s) 434
MseI TTAA 5 cut(s) 729, 852, 933, 1020, 1262
MspI CCGG 3 cut(s) 82, 677, 1295
MspR9I CCNGG 6 cut(s) 83, 307, 696, 703, 1204, 1271
MunI CAATTG 1 cut(s) 744
MvaI CCWGG 5 cut(s) 307, 696, 703, 1204, 1271
MvnI CGCG 1 cut(s) 1162
NarI GGCGCC 1 cut(s) 79
NciI CCSGG 1 cut(s) 83
NcoI CCATGG 1 cut(s) 1345
NdeI CATATG 1 cut(s) 736
NdeII GATC 4 cut(s) 209, 625, 698, 781
NlaIII CATG 9 cut(s) 368, 432, 571, 618, 827, 916, 1256, 1349, 1375
NlaIV GGNNCC 4 cut(s) 80, 257, 700, 1047
NsiI ATGCAT 1 cut(s) 434
NspI RCATGY 1 cut(s) 432
PaeI GCATGC 1 cut(s) 432
PaqCI CACCTGC 1 cut(s) 485
PciSI GCTCTTC 1 cut(s) 86
PfeI GAWTC 6 cut(s) 130, 332, 338, 664, 1087, 1217
PflFI GACNNNGTC 1 cut(s) 62
PfoI TCCNGGA 2 cut(s) 694, 1202
PinAI ACCGGT 1 cut(s) 676
PkrI GCNGC 3 cut(s) 78, 193, 427
PleI GAGTC 4 cut(s) 71, 308, 924, 1045
PluTI GGCGCC 1 cut(s) 82
PpsI GAGTC 4 cut(s) 71, 308, 924, 1045
PpuMI RGGWCCY 1 cut(s) 296
PsiI TTATAA 2 cut(s) 119, 1140
Psp124BI GAGCTC 2 cut(s) 43, 1101
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 5 cut(s) 305, 694, 701, 1202, 1269
PspGI CCWGG 5 cut(s) 305, 694, 701, 1202, 1269
PspN4I GGNNCC 4 cut(s) 80, 257, 700, 1047
PspPI GGNCC 1 cut(s) 296
PspPPI RGGWCCY 1 cut(s) 296
PsuI RGATCY 2 cut(s) 209, 698
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 4 cut(s) 363, 635, 741, 1248
RsaNI GTAC 4 cut(s) 362, 634, 740, 1247
SacI GAGCTC 2 cut(s) 43, 1101
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 5 cut(s) 729, 852, 933, 1020, 1262
SatI GCNGC 3 cut(s) 77, 192, 426
Sau3AI GATC 4 cut(s) 209, 625, 698, 781
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 635
SchI GAGTC 4 cut(s) 72, 308, 925, 1046
ScrFI CCNGG 6 cut(s) 83, 307, 696, 703, 1204, 1271
SduI GDGCHC 3 cut(s) 43, 502, 1101
SexAI ACCWGGT 1 cut(s) 305
SfaNI GCATC 1 cut(s) 437
SfoI GGCGCC 1 cut(s) 80
SinI GGWCC 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
SphI GCATGC 1 cut(s) 432
Sse9I AATT 7 cut(s) 381, 404, 744, 946, 980, 1068, 1174
SsiI CCGC 3 cut(s) 76, 111, 1115
SspDI GGCGCC 1 cut(s) 78
SspMI CTAG 2 cut(s) 357, 512
SstI GAGCTC 2 cut(s) 43, 1101
StyD4I CCNGG 6 cut(s) 81, 305, 694, 701, 1202, 1269
StyI CCWWGG 4 cut(s) 292, 770, 889, 1345
TaaI ACNGT 3 cut(s) 150, 263, 861
TaiI ACGT 1 cut(s) 505
TaqI TCGA 3 cut(s) 57, 486, 594
TasI AATT 7 cut(s) 381, 404, 744, 946, 980, 1068, 1174
TatI WGTACW 2 cut(s) 361, 633
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 6 cut(s) 130, 332, 338, 664, 1087, 1217
Tru1I TTAA 5 cut(s) 729, 852, 933, 1020, 1262
Tru9I TTAA 5 cut(s) 729, 852, 933, 1020, 1262
TscAI CASTG 1 cut(s) 1158
TseI GCWGC 2 cut(s) 191, 425
TspDTI ATGAA 6 cut(s) 224, 432, 471, 483, 603, 1241
TspGWI ACGGA 2 cut(s) 632, 676
TspRI CASTG 1 cut(s) 1158
Tth111I GACNNNGTC 1 cut(s) 62
VneI GTGCAC 1 cut(s) 498
VpaK11BI GGWCC 1 cut(s) 296
XagI CCTNNNNNAGG 1 cut(s) 651
XapI RAATTY 3 cut(s) 946, 980, 1068
XceI RCATGY 1 cut(s) 432
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 2 cut(s) 357, 512
ZrmI AGTACT 1 cut(s) 635
Zsp2I ATGCAT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.