Rw0G016980

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0002719
Physical Location & Seq
Reverse (-)
638666 .. 640731
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016980.1

Sequence Viewer

Length: 1461 bp
ATGGGTGTATGTGTCTCAAAGCCAAGTGAAACTATCAGAGCTCAAAAAAAAAAGAAGAGCAAGAAGGTAGCCACCGCCGTTATAAACTCAGAGATTCCTGCTCCGACGAGACAGTCTGTTAGTGAGTTTGTGCGTCTTGACTTTGAGAAAGGTGCTGCAACCACTTGCAGAAGATCTGAGGTTTCCAATGGCACATTTCATCTAACTCAACTTCAATGGAACCACAGTCAGATTGATGGGAATGGTGGGAAATGCCAAGGACCTGAAACCTGGTTTGACTCCCATAGTATAATGGAATCTGATTCAGATGATGACTTTGCTAGTGTACATGGAGATGAGTTTCCTCCTATGGTAGTAGGTAATGGAATTGGAAACATCCCAAATAGTCAATTAGTCCAGTATGAAAGTGCAGCATGCATTGTAGATAATGGATGCAAGTATGAAGGGTTTTATGAAAGTTATCTGAAAATCGATGGGGCAGGTGCACGTCATTCCCTAGAGAAAACCCAAGTCAGTTACAATGACAAAAATCAGCCCAAGCAATCAGTTATCATGCTTTCCTACAAGAGAAAATCCATCGACAACCCTGAAAGGATTTCATGTGTTTCTGATCAGAAGTACTTATTCCGTCCTAAATCAGGACAACGGATTCCTTGCTCACCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAATCTTTAAGCTCCGTGGGGAGAACTATTTCAAAGACAAACAGAAGTACCCTGCTCCAAACTGCAGCTCATATGTACCAATTGGTATTGATATGTTTATCTGCCCAAGGAAGAGGGATCACATTGCTCAGTATCTTGAGCTTCCTTCTGTAAAACCACATGATAAAGTTCCTTCCCTCCTCATTGTTAATATACAGTTGCCTACATATCCAACTACAATGTTCCTTGGTGAAAGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTTAGTGAAAATTTTGACACAGACATATCTCCTCAGTTTCAAGAATTTATCAAGAGATTTGTTGAGGATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATTGGTTCCTTTCAGGGAAAGGCTAAAAATTTTGGCTGGATTGGTGAATCCAGACGAGCTCCAACTGAGTTCTGCTGAGAAGAAGCTGATAAGTGCTTATAATGATAAACCAGTGCTTTCGCGTCCTCAACACAATTTCTTCAGGGGGCCTAATTACTTTGAAATTGATCTAGACATACACAGGTTCAGCTACATCTCCAGGAAAGGGCTTGAATCATTCAGAGACAGATTGAAGAATGGGGTACTTCATGTGGGATTAACAATCCAGGCACAAAAACAAGAAGAACTACCGGAGCAAGTCTTGTGTTGTATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGAATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

486

Amino Acids

55.01

Weight (kDa)

7.14

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 230 - 471 2e-60 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 83, 1182
AarI CACCTGC 1 cut(s) 470
AasI GACNNNNNNGTC 1 cut(s) 112
Acc36I ACCTGC 1 cut(s) 470
AccII CGCG 1 cut(s) 1204
AciI CCGC 1 cut(s) 75
AclWI GGATC 3 cut(s) 678, 691, 831
AcsI RAATTY 3 cut(s) 988, 1022, 1110
AcuI CTGAAG 1 cut(s) 1207
AfaI GTAC 5 cut(s) 327, 620, 755, 783, 1326
AfiI CCNNNNNNNGG 3 cut(s) 638, 680, 1287
AgeI ACCGGT 1 cut(s) 661
AgsI TTSAA 8 cut(s) 215, 708, 739, 1019, 1244, 1295, 1315, 1402
AjiI CACGTC 1 cut(s) 488
AjnI CCWGG 5 cut(s) 269, 679, 686, 1280, 1347
AluBI AGCT 7 cut(s) 41, 718, 774, 847, 1141, 1168, 1272
AluI AGCT 7 cut(s) 41, 718, 774, 847, 1141, 1168, 1272
Alw21I GWGCWC 3 cut(s) 43, 487, 1143
Alw26I GTCTC 3 cut(s) 19, 103, 1299
Alw44I GTGCAC 1 cut(s) 483
AlwI GGATC 3 cut(s) 678, 691, 831
AoxI GGCC 1 cut(s) 1229
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 3 cut(s) 155, 410, 771
ApoI RAATTY 3 cut(s) 988, 1022, 1110
AsiGI ACCGGT 1 cut(s) 661
Asp700I GAANNNNTTC 1 cut(s) 734
AspLEI GCGC 1 cut(s) 1398
AspS9I GGNCC 2 cut(s) 260, 1229
AsuHPI GGTGA 4 cut(s) 651, 947, 959, 1138
AvaII GGWCC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 487
BaeI ACNNNNGTAYC 2 cut(s) 737, 770
BamHI GGATCC 1 cut(s) 683
BanII GRGCYC 2 cut(s) 43, 1143
Bbv12I GWGCWC 3 cut(s) 43, 487, 1143
BbvI GCAGC 3 cut(s) 142, 422, 783
BccI CCATC 4 cut(s) 230, 467, 584, 938
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 5 cut(s) 271, 681, 688, 1282, 1349
BclI TGATCA 1 cut(s) 610
BcoDI GTCTC 3 cut(s) 19, 103, 1299
BfaI CTAG 3 cut(s) 321, 497, 1253
BfmI CTRYAG 1 cut(s) 769
BfuAI ACCTGC 1 cut(s) 470
BglII AGATCT 1 cut(s) 173
BisI GCNGC 3 cut(s) 156, 411, 772
BlsI GCNGC 3 cut(s) 157, 412, 773
BmcAI AGTACT 1 cut(s) 620
Bme1390I CCNGG 5 cut(s) 271, 681, 688, 1282, 1349
Bme18I GGWCC 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 488
BmgT120I GGNCC 2 cut(s) 260, 1229
BmiI GGNNCC 4 cut(s) 221, 685, 1089, 1230
BmrFI CCNGG 5 cut(s) 271, 681, 688, 1282, 1349
BmsI GCATC 1 cut(s) 422
BpmI CTGGAG 2 cut(s) 663, 1264
BpuEI CTTGAG 1 cut(s) 863
Bsa29I ATCGAT 1 cut(s) 471
BsaJI CCNNGG 5 cut(s) 256, 721, 812, 931, 1423
BsaWI WCCGGW 2 cut(s) 661, 1372
Bsc4I CCNNNNNNNGG 3 cut(s) 638, 680, 1287
Bse118I RCCGGY 1 cut(s) 661
Bse1I ACTGG 2 cut(s) 397, 1193
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 5 cut(s) 271, 681, 688, 1282, 1349
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 5 cut(s) 256, 721, 812, 931, 1423
BseGI GGATG 3 cut(s) 375, 437, 1051
BseLI CCNNNNNNNGG 3 cut(s) 638, 680, 1287
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 6 cut(s) 102, 168, 848, 1025, 1139, 1149
BseNI ACTGG 2 cut(s) 397, 1193
BseRI GAGGAG 2 cut(s) 875, 999
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 3 cut(s) 142, 422, 783
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 1204
BshFI GGCC 1 cut(s) 1231
BshTI ACCGGT 1 cut(s) 661
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 3 cut(s) 43, 487, 1143
BsiSI CCGG 2 cut(s) 662, 1373
BslI CCNNNNNNNGG 3 cut(s) 638, 680, 1287
BsmAI GTCTC 3 cut(s) 19, 103, 1299
BsnI GGCC 1 cut(s) 1231
Bsp1286I GDGCHC 3 cut(s) 43, 487, 1143
Bsp1407I TGTACA 1 cut(s) 325
Bsp143I GATC 5 cut(s) 173, 610, 683, 823, 1249
Bsp19I CCATGG 1 cut(s) 1423
BspACI CCGC 1 cut(s) 75
BspANI GGCC 1 cut(s) 1231
BspCNI CTCAG 6 cut(s) 101, 169, 847, 1024, 1140, 1150
BspDI ATCGAT 1 cut(s) 471
BspFNI CGCG 1 cut(s) 1204
BspLI GGNNCC 4 cut(s) 221, 685, 1089, 1230
BspMAI CTGCAG 1 cut(s) 773
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 3 cut(s) 678, 691, 831
BspQI GCTCTTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 661
BsrGI TGTACA 1 cut(s) 325
BsrI ACTGG 2 cut(s) 397, 1193
BssAI RCCGGY 1 cut(s) 661
BssECI CCNNGG 5 cut(s) 256, 721, 812, 931, 1423
BssMI GATC 5 cut(s) 173, 610, 683, 823, 1249
BssT1I CCWWGG 4 cut(s) 256, 812, 931, 1423
Bst2UI CCWGG 5 cut(s) 271, 681, 688, 1282, 1349
Bst4CI ACNGT 3 cut(s) 114, 227, 903
Bst6I CTCTTC 2 cut(s) 50, 812
BstAUI TGTACA 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 6 cut(s) 88, 177, 834, 1011, 1148, 1158
BstDSI CCRYGG 2 cut(s) 721, 1423
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 3 cut(s) 375, 437, 1051
BstFNI CGCG 1 cut(s) 1204
BstHHI GCGC 1 cut(s) 1398
BstKTI GATC 5 cut(s) 176, 613, 686, 826, 1252
BstMAI GTCTC 3 cut(s) 19, 103, 1299
BstMBI GATC 5 cut(s) 173, 610, 683, 823, 1249
BstNI CCWGG 5 cut(s) 271, 681, 688, 1282, 1349
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 5 cut(s) 269, 679, 686, 1280, 1347
BstSFI CTRYAG 1 cut(s) 769
BstSLI GKGCMC 1 cut(s) 487
BstUI CGCG 1 cut(s) 1204
BstV1I GCAGC 3 cut(s) 142, 422, 783
BstX2I RGATCY 2 cut(s) 173, 683
BstXI CCANNNNNNTGG 1 cut(s) 687
BstYI RGATCY 2 cut(s) 173, 683
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 1 cut(s) 1231
BsuTUI ATCGAT 1 cut(s) 471
BtgI CCRYGG 2 cut(s) 721, 1423
BtrI CACGTC 1 cut(s) 488
BtsCI GGATG 3 cut(s) 375, 437, 1051
BtsIMutI CAGTG 1 cut(s) 1200
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 415
CfoI GCGC 1 cut(s) 1398
Cfr10I RCCGGY 1 cut(s) 661
Cfr13I GGNCC 2 cut(s) 260, 1229
ClaI ATCGAT 1 cut(s) 471
CseI GACGC 2 cut(s) 122, 1193
CsiI ACCWGGT 1 cut(s) 269
Csp6I GTAC 5 cut(s) 326, 619, 754, 782, 1325
CspAI ACCGGT 1 cut(s) 661
CviAII CATG 9 cut(s) 329, 414, 553, 600, 866, 955, 1331, 1424, 1450
CviQI GTAC 5 cut(s) 326, 619, 754, 782, 1325
DdeI CTNAG 6 cut(s) 88, 177, 834, 1011, 1148, 1158
DpnI GATC 5 cut(s) 175, 612, 685, 825, 1251
DpnII GATC 5 cut(s) 173, 610, 683, 823, 1249
DraI TTTAAA 1 cut(s) 976
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 50, 812
EarI CTCTTC 2 cut(s) 50, 812
Ecl136II GAGCTC 2 cut(s) 41, 1141
Eco130I CCWWGG 4 cut(s) 256, 812, 931, 1423
Eco24I GRGCYC 2 cut(s) 43, 1143
Eco47I GGWCC 1 cut(s) 260
Eco53kI GAGCTC 2 cut(s) 41, 1141
Eco57I CTGAAG 1 cut(s) 1207
EcoICRI GAGCTC 2 cut(s) 41, 1141
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoO109I RGGNCCY 2 cut(s) 260, 1229
EcoRII CCWGG 5 cut(s) 269, 679, 686, 1280, 1347
EcoT14I CCWWGG 4 cut(s) 256, 812, 931, 1423
EcoT22I ATGCAT 1 cut(s) 419
EcoT38I GRGCYC 2 cut(s) 43, 1143
ErhI CCWWGG 4 cut(s) 256, 812, 931, 1423
FaeI CATG 9 cut(s) 332, 417, 556, 603, 869, 958, 1334, 1427, 1453
FatI CATG 9 cut(s) 328, 413, 552, 599, 865, 954, 1330, 1423, 1449
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 610
Fnu4HI GCNGC 3 cut(s) 156, 411, 772
FokI GGATG 3 cut(s) 362, 444, 1058
FriOI GRGCYC 2 cut(s) 43, 1143
Fsp4HI GCNGC 3 cut(s) 156, 411, 772
FspBI CTAG 3 cut(s) 321, 497, 1253
GlaI GCGC 1 cut(s) 1397
GluI GCNGC 3 cut(s) 156, 411, 772
GsuI CTGGAG 2 cut(s) 663, 1264
HaeIII GGCC 1 cut(s) 1231
HapII CCGG 2 cut(s) 662, 1373
HgaI GACGC 2 cut(s) 122, 1193
HhaI GCGC 1 cut(s) 1398
Hin1II CATG 9 cut(s) 332, 417, 556, 603, 869, 958, 1334, 1427, 1453
Hin6I GCGC 1 cut(s) 1396
HinP1I GCGC 1 cut(s) 1396
HinfI GANTC 9 cut(s) 94, 278, 296, 302, 649, 958, 1079, 1129, 1295
HpaII CCGG 2 cut(s) 662, 1373
HphI GGTGA 4 cut(s) 651, 947, 959, 1138
Hpy166II GTNNAC 4 cut(s) 326, 485, 1071, 1420
Hpy188III TCNNGA 7 cut(s) 137, 639, 842, 1019, 1030, 1133, 1253
Hpy8I GTNNAC 4 cut(s) 326, 485, 1071, 1420
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 6 cut(s) 58, 437, 714, 861, 888, 944
HpyCH4III ACNGT 3 cut(s) 114, 227, 903
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 7 cut(s) 158, 168, 410, 417, 435, 485, 771
HpyF3I CTNAG 6 cut(s) 88, 177, 834, 1011, 1148, 1158
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 9 cut(s) 332, 417, 556, 603, 869, 958, 1334, 1427, 1453
HspAI GCGC 1 cut(s) 1396
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 5 cut(s) 173, 610, 683, 823, 1249
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 5 cut(s) 106, 723, 766, 1146, 1375
Lsp1109I GCAGC 3 cut(s) 142, 422, 783
LweI GCATC 1 cut(s) 422
MabI ACCWGGT 1 cut(s) 269
MaeI CTAG 3 cut(s) 321, 497, 1253
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 2 cut(s) 515, 1444
MalI GATC 5 cut(s) 175, 612, 685, 825, 1251
MboI GATC 5 cut(s) 173, 610, 683, 823, 1249
MboII GAAGA 7 cut(s) 67, 183, 829, 1174, 1213, 1327, 1376
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 2 cut(s) 173, 683
MhlI GDGCHC 3 cut(s) 43, 487, 1143
MluCI AATT 9 cut(s) 366, 389, 786, 988, 1022, 1110, 1216, 1234, 1245
MlyI GAGTC 3 cut(s) 272, 967, 1088
MmeI TCCRAC 3 cut(s) 128, 941, 1168
MnlI CCTC 8 cut(s) 172, 354, 813, 893, 896, 1020, 1036, 1218
Mph1103I ATGCAT 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 734
MseI TTAA 5 cut(s) 714, 894, 975, 1062, 1340
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 2 cut(s) 662, 1373
MspR9I CCNGG 5 cut(s) 271, 681, 688, 1282, 1349
MunI CAATTG 1 cut(s) 786
MvaI CCWGG 5 cut(s) 271, 681, 688, 1282, 1349
MvnI CGCG 1 cut(s) 1204
NcoI CCATGG 1 cut(s) 1423
NdeI CATATG 1 cut(s) 778
NdeII GATC 5 cut(s) 173, 610, 683, 823, 1249
NlaIII CATG 9 cut(s) 332, 417, 556, 603, 869, 958, 1334, 1427, 1453
NlaIV GGNNCC 4 cut(s) 221, 685, 1089, 1230
NsiI ATGCAT 1 cut(s) 419
NspI RCATGY 1 cut(s) 417
PaeI GCATGC 1 cut(s) 417
PaqCI CACCTGC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 734
PfeI GAWTC 6 cut(s) 94, 296, 302, 649, 1129, 1295
PfoI TCCNGGA 2 cut(s) 679, 1280
PinAI ACCGGT 1 cut(s) 661
PkrI GCNGC 3 cut(s) 157, 412, 773
PleI GAGTC 3 cut(s) 272, 966, 1087
PpsI GAGTC 3 cut(s) 272, 966, 1087
PpuMI RGGWCCY 1 cut(s) 260
PsiI TTATAA 2 cut(s) 83, 1182
Psp124BI GAGCTC 2 cut(s) 43, 1143
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 5 cut(s) 269, 679, 686, 1280, 1347
PspGI CCWGG 5 cut(s) 269, 679, 686, 1280, 1347
PspN4I GGNNCC 4 cut(s) 221, 685, 1089, 1230
PspPI GGNCC 2 cut(s) 260, 1229
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 773
PsuI RGATCY 2 cut(s) 173, 683
RsaI GTAC 5 cut(s) 327, 620, 755, 783, 1326
RsaNI GTAC 5 cut(s) 326, 619, 754, 782, 1325
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 2 cut(s) 43, 1143
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 5 cut(s) 714, 894, 975, 1062, 1340
SatI GCNGC 3 cut(s) 156, 411, 772
Sau3AI GATC 5 cut(s) 173, 610, 683, 823, 1249
Sau96I GGNCC 2 cut(s) 260, 1229
ScaI AGTACT 1 cut(s) 620
SchI GAGTC 3 cut(s) 272, 967, 1088
ScrFI CCNGG 5 cut(s) 271, 681, 688, 1282, 1349
SduI GDGCHC 3 cut(s) 43, 487, 1143
SexAI ACCWGGT 1 cut(s) 269
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 1 cut(s) 769
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
SphI GCATGC 1 cut(s) 417
Sse9I AATT 9 cut(s) 366, 389, 786, 988, 1022, 1110, 1216, 1234, 1245
SsiI CCGC 1 cut(s) 75
SspMI CTAG 3 cut(s) 321, 497, 1253
SstI GAGCTC 2 cut(s) 43, 1143
StyD4I CCNGG 5 cut(s) 269, 679, 686, 1280, 1347
StyI CCWWGG 4 cut(s) 256, 812, 931, 1423
TaaI ACNGT 3 cut(s) 114, 227, 903
TaiI ACGT 1 cut(s) 490
TaqI TCGA 2 cut(s) 471, 579
TasI AATT 9 cut(s) 366, 389, 786, 988, 1022, 1110, 1216, 1234, 1245
TatI WGTACW 2 cut(s) 325, 618
TfiI GAWTC 6 cut(s) 94, 296, 302, 649, 1129, 1295
Tru1I TTAA 5 cut(s) 714, 894, 975, 1062, 1340
Tru9I TTAA 5 cut(s) 714, 894, 975, 1062, 1340
TscAI CASTG 1 cut(s) 1200
TseI GCWGC 3 cut(s) 155, 410, 771
TspDTI ATGAA 6 cut(s) 188, 417, 456, 468, 588, 1319
TspGWI ACGGA 3 cut(s) 617, 661, 710
TspRI CASTG 1 cut(s) 1200
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 636
XapI RAATTY 3 cut(s) 988, 1022, 1110
XbaI TCTAGA 1 cut(s) 1252
XceI RCATGY 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 734
XspI CTAG 3 cut(s) 321, 497, 1253
ZrmI AGTACT 1 cut(s) 620
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.