Rh4BG328600

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002719
Physical Location & Seq
Reverse (-)
49453229 .. 49455895
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG328600.1

Sequence Viewer

Length: 975 bp
ATGACAAAAATTGATCAGCCCAAGCAATCCGCAGTTATCATGCTTTCCTACAGGAGAAAATCCATCGACAACCCCGAAAGGACTTCATGTGCTTCTGATCAGAAGTACTTATTCCGCCCTAAAGCAGGACTACGCATTCCTTGTTCAGCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTTAAGCTCCGCGGGGAGAACTATTTCAAAGACAAACAGAAGTACCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTATTGATTTGTTTATCTGCCCAAGGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAGAACCACATGACAAAGTTCCTTCCCTCCTTATTGTTAATATACAGTTTCCTACATATCCTATTACAATGTTCCCTAGTGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTCAGTGAAAATTTTGACAAAGACATCTCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGGATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATCGGTTCCTTTCAGGGAAAGGCTAAAAATTATGGCTGGATTGGTGAATCCAGACGAGGTCCAACTGAGTTCTGCTGAGAAGAAGCTTTTAAGTGCTTATAATGATAAACCAGTGCTTTCGCGCCCTCAACACAATTTCTTCAGGGGACCTAATTACTTTGAGATTGACCTAGACATACACACGTTCAGCTACATCTCCAGGAAAGGGCTTGAATCATCCCGAGACAGATTGAAGAATGGGGTATTTAATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAATTACCGGAGCAAGTCTTGTGTTGTATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTCACTTGGACAAGCACCTGTGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

324

Amino Acids

37.01

Weight (kDa)

7.59

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 59 - 300 9.1e-61 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AccII CGCG 2 cut(s) 210, 691
AciI CCGC 5 cut(s) 30, 115, 149, 208, 210
AclWI GGATC 2 cut(s) 165, 178
AcsI RAATTY 2 cut(s) 475, 509
AcuI CTGAAG 2 cut(s) 177, 694
AcyI GRCGYC 1 cut(s) 968
AfaI GTAC 3 cut(s) 107, 242, 270
AfiI CCNNNNNNNGG 3 cut(s) 125, 167, 774
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 5 cut(s) 226, 506, 782, 802, 889
AjnI CCWGG 4 cut(s) 166, 173, 767, 834
AluBI AGCT 5 cut(s) 205, 261, 334, 655, 759
AluI AGCT 5 cut(s) 205, 261, 334, 655, 759
Alw26I GTCTC 1 cut(s) 786
AlwI GGATC 2 cut(s) 165, 178
Ama87I CYCGRG 1 cut(s) 789
ApoI RAATTY 2 cut(s) 475, 509
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 2 cut(s) 693, 885
AspS9I GGNCC 2 cut(s) 628, 716
AsuHPI GGTGA 2 cut(s) 446, 625
AvaI CYCGRG 1 cut(s) 789
AvaII GGWCC 2 cut(s) 628, 716
BaeI ACNNNNGTAYC 2 cut(s) 224, 257
BamHI GGATCC 1 cut(s) 170
BccI CCATC 2 cut(s) 71, 425
BciT130I CCWGG 4 cut(s) 168, 175, 769, 836
BclI TGATCA 2 cut(s) 13, 97
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 2 cut(s) 420, 740
BfmI CTRYAG 3 cut(s) 49, 256, 342
BlpI GCTNAGC 1 cut(s) 321
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 4 cut(s) 168, 175, 769, 836
Bme18I GGWCC 2 cut(s) 628, 716
BmeT110I CYCGRG 1 cut(s) 789
BmgT120I GGNCC 2 cut(s) 628, 716
BmiI GGNNCC 3 cut(s) 172, 576, 717
BmrFI CCNGG 4 cut(s) 168, 175, 769, 836
BmsI GCATC 1 cut(s) 334
BpmI CTGGAG 2 cut(s) 150, 751
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 968
BsaJI CCNNGG 4 cut(s) 208, 299, 910, 936
BsaWI WCCGGW 1 cut(s) 859
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 167, 774
Bse1I ACTGG 1 cut(s) 680
Bse3DI GCAATG 1 cut(s) 315
BseBI CCWGG 4 cut(s) 168, 175, 769, 836
BseDI CCNNGG 4 cut(s) 208, 299, 910, 936
BseGI GGATG 3 cut(s) 538, 785, 946
BseLI CCNNNNNNNGG 3 cut(s) 125, 167, 774
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 4 cut(s) 335, 512, 626, 636
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 1 cut(s) 486
Bsh1236I CGCG 2 cut(s) 210, 691
BsiHKCI CYCGRG 1 cut(s) 789
BsiSI CCGG 1 cut(s) 860
BslFI GGGAC 1 cut(s) 729
BslI CCNNNNNNNGG 3 cut(s) 125, 167, 774
BsmAI GTCTC 1 cut(s) 786
BsmFI GGGAC 1 cut(s) 729
BsmI GAATGC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 789
Bsp143I GATC 4 cut(s) 13, 97, 170, 310
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 2 cut(s) 910, 936
BspACI CCGC 5 cut(s) 30, 115, 149, 208, 210
BspCNI CTCAG 4 cut(s) 334, 511, 627, 637
BspFNI CGCG 2 cut(s) 210, 691
BspLI GGNNCC 3 cut(s) 172, 576, 717
BspPI GGATC 2 cut(s) 165, 178
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 1 cut(s) 680
BssECI CCNNGG 4 cut(s) 208, 299, 910, 936
BssMI GATC 4 cut(s) 13, 97, 170, 310
BssNI GRCGYC 1 cut(s) 968
BssT1I CCWWGG 3 cut(s) 299, 910, 936
Bst2UI CCWGG 4 cut(s) 168, 175, 769, 836
Bst4CI ACNGT 1 cut(s) 390
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 968
BstDEI CTNAG 4 cut(s) 321, 498, 635, 645
BstDSI CCRYGG 3 cut(s) 208, 910, 936
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 538, 785, 946
BstFNI CGCG 2 cut(s) 210, 691
BstHHI GCGC 2 cut(s) 693, 885
BstKTI GATC 4 cut(s) 16, 100, 173, 313
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 4 cut(s) 13, 97, 170, 310
BstMWI GCNNNNNNNGC 2 cut(s) 331, 965
BstNI CCWGG 4 cut(s) 168, 175, 769, 836
BstSCI CCNGG 4 cut(s) 166, 173, 767, 834
BstSFI CTRYAG 3 cut(s) 49, 256, 342
BstUI CGCG 2 cut(s) 210, 691
BstX2I RGATCY 1 cut(s) 170
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 1 cut(s) 170
BtgI CCRYGG 3 cut(s) 208, 910, 936
BtsCI GGATG 3 cut(s) 538, 785, 946
BtsIMutI CAGTG 2 cut(s) 475, 687
CfoI GCGC 2 cut(s) 693, 885
Cfr13I GGNCC 2 cut(s) 628, 716
Cfr42I CCGCGG 1 cut(s) 211
CseI GACGC 1 cut(s) 957
Csp6I GTAC 3 cut(s) 106, 241, 269
CviAII CATG 6 cut(s) 40, 87, 353, 442, 911, 937
CviQI GTAC 3 cut(s) 106, 241, 269
DdeI CTNAG 4 cut(s) 321, 498, 635, 645
DpnI GATC 4 cut(s) 15, 99, 172, 312
DpnII GATC 4 cut(s) 13, 97, 170, 310
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
EciI GGCGGA 1 cut(s) 104
Eco130I CCWWGG 3 cut(s) 299, 910, 936
Eco47I GGWCC 2 cut(s) 628, 716
Eco57I CTGAAG 2 cut(s) 177, 694
Eco88I CYCGRG 1 cut(s) 789
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 716
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 4 cut(s) 166, 173, 767, 834
EcoT14I CCWWGG 3 cut(s) 299, 910, 936
ErhI CCWWGG 3 cut(s) 299, 910, 936
FaeI CATG 6 cut(s) 43, 90, 356, 445, 914, 940
FaqI GGGAC 1 cut(s) 729
FatI CATG 6 cut(s) 39, 86, 352, 441, 910, 936
FauI CCCGC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 265
FbaI TGATCA 2 cut(s) 13, 97
FokI GGATG 3 cut(s) 545, 772, 953
FspBI CTAG 2 cut(s) 420, 740
GlaI GCGC 2 cut(s) 692, 884
GsuI CTGGAG 2 cut(s) 150, 751
HapII CCGG 1 cut(s) 860
HgaI GACGC 1 cut(s) 957
HhaI GCGC 2 cut(s) 693, 885
Hin1I GRCGYC 1 cut(s) 968
Hin1II CATG 6 cut(s) 43, 90, 356, 445, 914, 940
Hin6I GCGC 2 cut(s) 691, 883
HinP1I GCGC 2 cut(s) 691, 883
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 5 cut(s) 445, 566, 616, 782, 944
HpaII CCGG 1 cut(s) 860
HphI GGTGA 2 cut(s) 446, 625
Hpy166II GTNNAC 2 cut(s) 558, 907
Hpy188I TCNGA 3 cut(s) 97, 102, 181
Hpy188III TCNNGA 6 cut(s) 329, 506, 517, 620, 789, 972
Hpy8I GTNNAC 2 cut(s) 558, 907
HpyAV CCTTC 4 cut(s) 201, 348, 375, 431
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 1 cut(s) 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 965
HpyF3I CTNAG 4 cut(s) 321, 498, 635, 645
HpySE526I ACGT 1 cut(s) 752
Hsp92I GRCGYC 1 cut(s) 968
Hsp92II CATG 6 cut(s) 43, 90, 356, 445, 914, 940
HspAI GCGC 2 cut(s) 691, 883
Ksp22I TGATCA 2 cut(s) 13, 97
KspI CCGCGG 1 cut(s) 211
Kzo9I GATC 4 cut(s) 13, 97, 170, 310
LmnI GCTCC 3 cut(s) 210, 253, 862
LweI GCATC 1 cut(s) 334
MaeI CTAG 2 cut(s) 420, 740
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 1 cut(s) 931
MalI GATC 4 cut(s) 15, 99, 172, 312
MboI GATC 4 cut(s) 13, 97, 170, 310
MboII GAAGA 5 cut(s) 316, 661, 700, 814, 863
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 170
MluCI AATT 9 cut(s) 9, 273, 475, 509, 597, 703, 721, 817, 854
MlyI GAGTC 3 cut(s) 454, 575, 938
MmeI TCCRAC 1 cut(s) 655
MnlI CCTC 5 cut(s) 380, 507, 523, 619, 705
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 7 cut(s) 201, 381, 462, 549, 659, 816, 827
MspA1I CMGCKG 2 cut(s) 149, 210
MspI CCGG 1 cut(s) 860
MspR9I CCNGG 4 cut(s) 168, 175, 769, 836
MunI CAATTG 1 cut(s) 273
Mva1269I GAATGC 1 cut(s) 135
MvaI CCWGG 4 cut(s) 168, 175, 769, 836
MvnI CGCG 2 cut(s) 210, 691
MwoI GCNNNNNNNGC 2 cut(s) 331, 965
NcoI CCATGG 2 cut(s) 910, 936
NdeI CATATG 1 cut(s) 265
NdeII GATC 4 cut(s) 13, 97, 170, 310
NlaIII CATG 6 cut(s) 43, 90, 356, 445, 914, 940
NlaIV GGNNCC 3 cut(s) 172, 576, 717
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 616, 782
PflFI GACNNNGTC 1 cut(s) 626
PfoI TCCNGGA 2 cut(s) 166, 767
PleI GAGTC 3 cut(s) 453, 574, 938
PpsI GAGTC 3 cut(s) 453, 574, 938
PpuMI RGGWCCY 1 cut(s) 716
PsiI TTATAA 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 4 cut(s) 166, 173, 767, 834
PspGI CCWGG 4 cut(s) 166, 173, 767, 834
PspN4I GGNNCC 3 cut(s) 172, 576, 717
PspPI GGNCC 2 cut(s) 628, 716
PspPPI RGGWCCY 1 cut(s) 716
PsuI RGATCY 1 cut(s) 170
PsyI GACNNNGTC 1 cut(s) 626
RsaI GTAC 3 cut(s) 107, 242, 270
RsaNI GTAC 3 cut(s) 106, 241, 269
SacII CCGCGG 1 cut(s) 211
SaqAI TTAA 7 cut(s) 201, 381, 462, 549, 659, 816, 827
Sau3AI GATC 4 cut(s) 13, 97, 170, 310
Sau96I GGNCC 2 cut(s) 628, 716
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 3 cut(s) 454, 575, 938
ScrFI CCNGG 4 cut(s) 168, 175, 769, 836
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 3 cut(s) 49, 256, 342
Sfr303I CCGCGG 1 cut(s) 211
SgrBI CCGCGG 1 cut(s) 211
SinI GGWCC 2 cut(s) 628, 716
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 9 cut(s) 9, 273, 475, 509, 597, 703, 721, 817, 854
SsiI CCGC 5 cut(s) 30, 115, 149, 208, 210
SspMI CTAG 2 cut(s) 420, 740
StyD4I CCNGG 4 cut(s) 166, 173, 767, 834
StyI CCWWGG 3 cut(s) 299, 910, 936
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 1 cut(s) 755
TaqI TCGA 1 cut(s) 66
TasI AATT 9 cut(s) 9, 273, 475, 509, 597, 703, 721, 817, 854
TatI WGTACW 1 cut(s) 105
TfiI GAWTC 2 cut(s) 616, 782
Tru1I TTAA 7 cut(s) 201, 381, 462, 549, 659, 816, 827
Tru9I TTAA 7 cut(s) 201, 381, 462, 549, 659, 816, 827
TscAI CASTG 2 cut(s) 475, 687
TspDTI ATGAA 2 cut(s) 75, 502
TspRI CASTG 2 cut(s) 475, 687
Tth111I GACNNNGTC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 628, 716
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 2 cut(s) 475, 509
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 2 cut(s) 420, 740
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.