Rh4AG321500

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002719
Physical Location & Seq
Reverse (-)
63401621 .. 63404351
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG321500.1

Sequence Viewer

Length: 972 bp
ATGTATGTACTCAAGTCAGCATGGTCTTCTGTTATTTCGAACTCACTCATTTATCATTTAGTACAACATTGCTTAAAATGTGCAGGTGCTTCTGATCAAAAGTACTTATTCCGTCCTAAAGCAGGACTACGCATTCCTTGTTCACCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTTAAGCTCCGTGGGGAGAACTATTTCAAAGACAAACAGAAGTGCCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTATTGATTTGTTTATCTGCCCAAGGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAGAACCACATGACAAAGTTCCTTCCCTCCTTATTGTTAATATACAGTTTCCTACATATCCTATTACAATGTTCCCTAGTGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTCAGTGAAAATTTTGACAAAGACATCTCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGAATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATCGGTTCCTTTCAGGGAAAGGCTAAAAATTATGGCTGGATTGGTGAATCCAGACGAGGTCCAACTGAGTTCTGCTGAGAAGAAGCTTTTAAGTGCTTATAATGATAAACCAGTGCTTTCGCGTCCTCAACACAATTTCTTCAGGGGACCTAATTATTTTGAGATTGACCTAGACATACACACGTTCAGCTACATCTCCAGGAAAGGGCTTGAATCATCCCGAGACAGATTGAAGAGTGGGGTATTTAATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAATTGCCGGAGCAAGTCTTGTGTTGCATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTCACTTGGACAAGCACCTGTGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

323

Amino Acids

36.7

Weight (kDa)

7.58

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 58 - 299 5.7e-61 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 74
AccII CGCG 1 cut(s) 688
AclWI GGATC 2 cut(s) 162, 175
AcsI RAATTY 2 cut(s) 472, 506
AcuI CTGAAG 2 cut(s) 174, 691
AcyI GRCGYC 1 cut(s) 965
AfaI GTAC 4 cut(s) 9, 63, 104, 267
AfiI CCNNNNNNNGG 3 cut(s) 122, 164, 771
AflIII ACRYGT 1 cut(s) 747
AgeI ACCGGT 1 cut(s) 145
AgsI TTSAA 5 cut(s) 223, 503, 779, 799, 886
AjnI CCWGG 4 cut(s) 163, 170, 764, 831
AluBI AGCT 5 cut(s) 202, 258, 331, 652, 756
AluI AGCT 5 cut(s) 202, 258, 331, 652, 756
Alw26I GTCTC 1 cut(s) 783
AlwI GGATC 2 cut(s) 162, 175
Ama87I CYCGRG 1 cut(s) 786
ApoI RAATTY 2 cut(s) 472, 506
AsiGI ACCGGT 1 cut(s) 145
Asp700I GAANNNNTTC 1 cut(s) 218
AspLEI GCGC 1 cut(s) 882
AspS9I GGNCC 2 cut(s) 625, 713
AsuHPI GGTGA 3 cut(s) 135, 443, 622
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 786
AvaII GGWCC 2 cut(s) 625, 713
BaeGI GKGCMC 1 cut(s) 242
BamHI GGATCC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 18
BccI CCATC 1 cut(s) 422
BciT130I CCWGG 4 cut(s) 165, 172, 766, 833
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 783
BfaI CTAG 2 cut(s) 417, 737
BfmI CTRYAG 2 cut(s) 253, 339
BfuAI ACCTGC 1 cut(s) 74
BlpI GCTNAGC 1 cut(s) 318
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 4 cut(s) 165, 172, 766, 833
Bme18I GGWCC 2 cut(s) 625, 713
BmeT110I CYCGRG 1 cut(s) 786
BmgT120I GGNCC 2 cut(s) 625, 713
BmiI GGNNCC 3 cut(s) 169, 573, 714
BmrFI CCNGG 4 cut(s) 165, 172, 766, 833
BmsI GCATC 1 cut(s) 331
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 147, 748
Bpu1102I GCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 965
BsaJI CCNNGG 4 cut(s) 205, 296, 907, 933
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 164, 771
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 677
Bse3DI GCAATG 2 cut(s) 67, 312
BseBI CCWGG 4 cut(s) 165, 172, 766, 833
BseDI CCNNGG 4 cut(s) 205, 296, 907, 933
BseGI GGATG 2 cut(s) 782, 943
BseLI CCNNNNNNNGG 3 cut(s) 122, 164, 771
BseMI GCAATG 2 cut(s) 67, 312
BseMII CTCAG 4 cut(s) 332, 509, 623, 633
BseNI ACTGG 1 cut(s) 677
BseRI GAGGAG 1 cut(s) 483
BseSI GKGCMC 1 cut(s) 242
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 688
BshTI ACCGGT 1 cut(s) 145
BsiHKCI CYCGRG 1 cut(s) 786
BsiSI CCGG 2 cut(s) 146, 857
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 3 cut(s) 122, 164, 771
BsmAI GTCTC 1 cut(s) 783
BsmFI GGGAC 1 cut(s) 726
BsmI GAATGC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 786
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 3 cut(s) 94, 167, 307
Bsp1720I GCTNAGC 1 cut(s) 318
Bsp19I CCATGG 2 cut(s) 907, 933
BspCNI CTCAG 4 cut(s) 331, 508, 624, 634
BspFNI CGCG 1 cut(s) 688
BspLI GGNNCC 3 cut(s) 169, 573, 714
BspMI ACCTGC 1 cut(s) 74
BspPI GGATC 2 cut(s) 162, 175
BspT104I TTCGAA 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 67, 312
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 4 cut(s) 205, 296, 907, 933
BssMI GATC 3 cut(s) 94, 167, 307
BssNI GRCGYC 1 cut(s) 965
BssT1I CCWWGG 3 cut(s) 296, 907, 933
Bst2UI CCWGG 4 cut(s) 165, 172, 766, 833
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 2 cut(s) 296, 794
BstACI GRCGYC 1 cut(s) 965
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 878
BstDEI CTNAG 4 cut(s) 318, 495, 632, 642
BstDSI CCRYGG 3 cut(s) 205, 907, 933
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 782, 943
BstFNI CGCG 1 cut(s) 688
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 3 cut(s) 97, 170, 310
BstMAI GTCTC 1 cut(s) 783
BstMBI GATC 3 cut(s) 94, 167, 307
BstMWI GCNNNNNNNGC 2 cut(s) 328, 962
BstNI CCWGG 4 cut(s) 165, 172, 766, 833
BstNSI RCATGY 1 cut(s) 880
BstSCI CCNGG 4 cut(s) 163, 170, 764, 831
BstSFI CTRYAG 2 cut(s) 253, 339
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 167
BstXI CCANNNNNNTGG 1 cut(s) 171
BstYI RGATCY 1 cut(s) 167
BtgI CCRYGG 3 cut(s) 205, 907, 933
BtsCI GGATG 2 cut(s) 782, 943
BtsIMutI CAGTG 2 cut(s) 472, 684
BveI ACCTGC 1 cut(s) 74
Cac8I GCNNGC 1 cut(s) 878
CfoI GCGC 1 cut(s) 882
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 625, 713
CseI GACGC 2 cut(s) 677, 954
Csp6I GTAC 4 cut(s) 8, 62, 103, 266
CspAI ACCGGT 1 cut(s) 145
CviAII CATG 6 cut(s) 21, 350, 439, 877, 908, 934
CviJI RGCY 9 cut(s) 157, 202, 258, 331, 589, 602, 652, 756, 775
CviKI_1 RGCY 9 cut(s) 157, 202, 258, 331, 589, 602, 652, 756, 775
CviQI GTAC 4 cut(s) 8, 62, 103, 266
DdeI CTNAG 4 cut(s) 318, 495, 632, 642
DpnI GATC 3 cut(s) 96, 169, 309
DpnII GATC 3 cut(s) 94, 167, 307
DraI TTTAAA 1 cut(s) 460
Eam1104I CTCTTC 2 cut(s) 296, 794
EarI CTCTTC 2 cut(s) 296, 794
Eco130I CCWWGG 3 cut(s) 296, 907, 933
Eco47I GGWCC 2 cut(s) 625, 713
Eco57I CTGAAG 2 cut(s) 174, 691
Eco88I CYCGRG 1 cut(s) 786
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 4 cut(s) 163, 170, 764, 831
EcoT14I CCWWGG 3 cut(s) 296, 907, 933
ErhI CCWWGG 3 cut(s) 296, 907, 933
FaeI CATG 6 cut(s) 24, 353, 442, 880, 911, 937
FaqI GGGAC 1 cut(s) 726
FatI CATG 6 cut(s) 20, 349, 438, 876, 907, 933
FauNDI CATATG 1 cut(s) 262
FbaI TGATCA 1 cut(s) 94
FokI GGATG 2 cut(s) 769, 950
FspBI CTAG 2 cut(s) 417, 737
GlaI GCGC 1 cut(s) 881
GsuI CTGGAG 2 cut(s) 147, 748
HapII CCGG 2 cut(s) 146, 857
HgaI GACGC 2 cut(s) 677, 954
HhaI GCGC 1 cut(s) 882
Hin1I GRCGYC 1 cut(s) 965
Hin1II CATG 6 cut(s) 24, 353, 442, 880, 911, 937
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 5 cut(s) 442, 563, 613, 779, 941
HpaII CCGG 2 cut(s) 146, 857
HphI GGTGA 3 cut(s) 135, 443, 622
Hpy166II GTNNAC 3 cut(s) 143, 555, 904
Hpy188I TCNGA 2 cut(s) 94, 178
Hpy188III TCNNGA 6 cut(s) 326, 503, 514, 617, 786, 969
Hpy8I GTNNAC 3 cut(s) 143, 555, 904
HpyAV CCTTC 4 cut(s) 198, 345, 372, 428
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 749
HpyCH4V TGCA 2 cut(s) 83, 876
HpyF10VI GCNNNNNNNGC 2 cut(s) 328, 962
HpyF3I CTNAG 4 cut(s) 318, 495, 632, 642
HpySE526I ACGT 1 cut(s) 749
Hsp92I GRCGYC 1 cut(s) 965
Hsp92II CATG 6 cut(s) 24, 353, 442, 880, 911, 937
HspAI GCGC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 3 cut(s) 94, 167, 307
LmnI GCTCC 3 cut(s) 207, 250, 859
LweI GCATC 1 cut(s) 331
MaeI CTAG 2 cut(s) 417, 737
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 1 cut(s) 928
MalI GATC 3 cut(s) 96, 169, 309
MboI GATC 3 cut(s) 94, 167, 307
MboII GAAGA 6 cut(s) 18, 313, 658, 697, 811, 860
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 8 cut(s) 270, 472, 506, 594, 700, 718, 814, 851
MlyI GAGTC 3 cut(s) 451, 572, 935
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 4 cut(s) 377, 504, 616, 702
MroXI GAANNNNTTC 1 cut(s) 218
MseI TTAA 8 cut(s) 74, 198, 378, 459, 546, 656, 813, 824
MspI CCGG 2 cut(s) 146, 857
MspR9I CCNGG 4 cut(s) 165, 172, 766, 833
MunI CAATTG 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 132
MvaI CCWGG 4 cut(s) 165, 172, 766, 833
MvnI CGCG 1 cut(s) 688
MwoI GCNNNNNNNGC 2 cut(s) 328, 962
NcoI CCATGG 2 cut(s) 907, 933
NdeI CATATG 1 cut(s) 262
NdeII GATC 3 cut(s) 94, 167, 307
NlaIII CATG 6 cut(s) 24, 353, 442, 880, 911, 937
NlaIV GGNNCC 3 cut(s) 169, 573, 714
NspI RCATGY 1 cut(s) 880
NspV TTCGAA 1 cut(s) 38
PaeI GCATGC 1 cut(s) 880
PaqCI CACCTGC 1 cut(s) 74
PctI GAATGC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 613, 779
PflFI GACNNNGTC 1 cut(s) 623
PfoI TCCNGGA 2 cut(s) 163, 764
PinAI ACCGGT 1 cut(s) 145
PleI GAGTC 3 cut(s) 450, 571, 935
PpsI GAGTC 3 cut(s) 450, 571, 935
PpuMI RGGWCCY 1 cut(s) 713
PsiI TTATAA 1 cut(s) 666
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 4 cut(s) 163, 170, 764, 831
PspGI CCWGG 4 cut(s) 163, 170, 764, 831
PspN4I GGNNCC 3 cut(s) 169, 573, 714
PspPI GGNCC 2 cut(s) 625, 713
PspPPI RGGWCCY 1 cut(s) 713
PsuI RGATCY 1 cut(s) 167
PsyI GACNNNGTC 1 cut(s) 623
RsaI GTAC 4 cut(s) 9, 63, 104, 267
RsaNI GTAC 4 cut(s) 8, 62, 103, 266
SaqAI TTAA 8 cut(s) 74, 198, 378, 459, 546, 656, 813, 824
Sau3AI GATC 3 cut(s) 94, 167, 307
Sau96I GGNCC 2 cut(s) 625, 713
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 3 cut(s) 451, 572, 935
ScrFI CCNGG 4 cut(s) 165, 172, 766, 833
SduI GDGCHC 1 cut(s) 242
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 2 cut(s) 253, 339
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 2 cut(s) 625, 713
SmlI CTYRAG 2 cut(s) 11, 326
SmoI CTYRAG 2 cut(s) 11, 326
SphI GCATGC 1 cut(s) 880
Sse9I AATT 8 cut(s) 270, 472, 506, 594, 700, 718, 814, 851
SspMI CTAG 2 cut(s) 417, 737
StyD4I CCNGG 4 cut(s) 163, 170, 764, 831
StyI CCWWGG 3 cut(s) 296, 907, 933
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 752
TaqI TCGA 1 cut(s) 38
TasI AATT 8 cut(s) 270, 472, 506, 594, 700, 718, 814, 851
TatI WGTACW 3 cut(s) 7, 61, 102
TfiI GAWTC 2 cut(s) 613, 779
Tru1I TTAA 8 cut(s) 74, 198, 378, 459, 546, 656, 813, 824
Tru9I TTAA 8 cut(s) 74, 198, 378, 459, 546, 656, 813, 824
TscAI CASTG 2 cut(s) 472, 684
TspDTI ATGAA 1 cut(s) 499
TspGWI ACGGA 2 cut(s) 101, 194
TspRI CASTG 2 cut(s) 472, 684
Tth111I GACNNNGTC 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 625, 713
XagI CCTNNNNNAGG 1 cut(s) 120
XapI RAATTY 2 cut(s) 472, 506
XceI RCATGY 1 cut(s) 880
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 2 cut(s) 417, 737
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.