Rw4G027840

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002719
Physical Location & Seq
Reverse (-)
54560656 .. 54562325
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027840.1

Sequence Viewer

Length: 1065 bp
ATGGAATCTGATTCAGATGTTGATTTTGCTAGTGTACATGGAGATGGGTTTCCTCCTATGGTAGTCTGTAATGGAATTGGAAACATCCCAAATAGTCAATTGGTCCACCCAAGCAATCCGCAGTTATCATGCTTTCCTACAGGGAGAAAATCCATCGACAACCCCGAAAGGACTTCATGTGCTTCTGATCAGAAGTACTTATTCCGTCCTAAAGCAGGACTACGCATTCCTTGTTCAGCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTTAAGCTCCGCGGGGAGAACTATTTCAAAGACAAACAGAAGTACCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTATTGATTTGTTTATCTGCCCAAGGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAGAACCACATGACAAAGTTCCTTCCCTCCTTATTGTTAATATACAGTTTCCTACATATCCTATTACAATGTTCCCTAGTGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTCAGTGAAAATTTTGACAAAGACATCTCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGGATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATCGGTTCCTTTCAGGGAAAGGCTAAAAATAATGGCTGGATTGGTTAATCCAGACGAGGTCCAACTGAGTTCTGCTGAGAAGAAGCTTTTAAGTGCTTATAACGATAAACCAGTGCTTTCGCGCCCTCAACACAATTTCTTCAAGGGACCTAATTATTTTGAGATTGACCTAGACATACACACGTTCAGCTACATCTCCAGGAAAGGGCTTGAGTCATCCCGAGACAGATTGAAGAATGGGGTATTTAATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAATTACCGGAGCAAGTCTTGTGTTGTATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTCACTTGGACAAGCACCTGTGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

354

Amino Acids

39.89

Weight (kDa)

5.81

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 89 - 330 1.7e-60 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AccII CGCG 2 cut(s) 300, 781
AciI CCGC 4 cut(s) 119, 239, 298, 300
AclWI GGATC 2 cut(s) 255, 268
AcsI RAATTY 2 cut(s) 565, 599
AcuI CTGAAG 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 1058
AfaI GTAC 4 cut(s) 36, 197, 332, 360
AfiI CCNNNNNNNGG 3 cut(s) 215, 257, 864
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 5 cut(s) 316, 596, 802, 892, 979
AjnI CCWGG 4 cut(s) 256, 263, 857, 924
AluBI AGCT 5 cut(s) 295, 351, 424, 745, 849
AluI AGCT 5 cut(s) 295, 351, 424, 745, 849
Alw26I GTCTC 1 cut(s) 876
AlwI GGATC 2 cut(s) 255, 268
Ama87I CYCGRG 1 cut(s) 879
ApoI RAATTY 2 cut(s) 565, 599
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 2 cut(s) 783, 975
AspS9I GGNCC 3 cut(s) 103, 718, 806
AsuHPI GGTGA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 879
AvaII GGWCC 3 cut(s) 103, 718, 806
BaeI ACNNNNGTAYC 2 cut(s) 314, 347
BamHI GGATCC 1 cut(s) 260
BccI CCATC 3 cut(s) 38, 161, 515
BciT130I CCWGG 4 cut(s) 258, 265, 859, 926
BclI TGATCA 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 3 cut(s) 30, 510, 830
BfmI CTRYAG 3 cut(s) 138, 346, 432
BlpI GCTNAGC 1 cut(s) 411
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 4 cut(s) 258, 265, 859, 926
Bme18I GGWCC 3 cut(s) 103, 718, 806
BmeT110I CYCGRG 1 cut(s) 879
BmgT120I GGNCC 3 cut(s) 103, 718, 806
BmiI GGNNCC 3 cut(s) 262, 666, 807
BmrFI CCNGG 4 cut(s) 258, 265, 859, 926
BmsI GCATC 1 cut(s) 424
BpmI CTGGAG 2 cut(s) 240, 841
Bpu1102I GCTNAGC 1 cut(s) 411
BpuEI CTTGAG 2 cut(s) 440, 890
BsaHI GRCGYC 1 cut(s) 1058
BsaJI CCNNGG 4 cut(s) 298, 389, 1000, 1026
BsaWI WCCGGW 1 cut(s) 949
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 257, 864
Bse1I ACTGG 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 4 cut(s) 258, 265, 859, 926
BseDI CCNNGG 4 cut(s) 298, 389, 1000, 1026
BseGI GGATG 4 cut(s) 84, 628, 875, 1036
BseLI CCNNNNNNNGG 3 cut(s) 215, 257, 864
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 4 cut(s) 425, 602, 716, 726
BseNI ACTGG 1 cut(s) 770
BseRI GAGGAG 1 cut(s) 576
Bsh1236I CGCG 2 cut(s) 300, 781
BsiHKCI CYCGRG 1 cut(s) 879
BsiSI CCGG 1 cut(s) 950
BslFI GGGAC 1 cut(s) 819
BslI CCNNNNNNNGG 3 cut(s) 215, 257, 864
BsmAI GTCTC 1 cut(s) 876
BsmFI GGGAC 1 cut(s) 819
BsmI GAATGC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 879
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 3 cut(s) 187, 260, 400
Bsp1720I GCTNAGC 1 cut(s) 411
Bsp19I CCATGG 2 cut(s) 1000, 1026
BspACI CCGC 4 cut(s) 119, 239, 298, 300
BspCNI CTCAG 4 cut(s) 424, 601, 717, 727
BspFNI CGCG 2 cut(s) 300, 781
BspLI GGNNCC 3 cut(s) 262, 666, 807
BspPI GGATC 2 cut(s) 255, 268
BsrDI GCAATG 1 cut(s) 405
BsrGI TGTACA 1 cut(s) 34
BsrI ACTGG 1 cut(s) 770
BssECI CCNNGG 4 cut(s) 298, 389, 1000, 1026
BssMI GATC 3 cut(s) 187, 260, 400
BssNI GRCGYC 1 cut(s) 1058
BssT1I CCWWGG 3 cut(s) 389, 1000, 1026
Bst2UI CCWGG 4 cut(s) 258, 265, 859, 926
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 1 cut(s) 389
BstACI GRCGYC 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 34
BstDEI CTNAG 4 cut(s) 411, 588, 725, 735
BstDSI CCRYGG 3 cut(s) 298, 1000, 1026
BstENI CCTNNNNNAGG 1 cut(s) 213
BstF5I GGATG 4 cut(s) 84, 628, 875, 1036
BstFNI CGCG 2 cut(s) 300, 781
BstHHI GCGC 2 cut(s) 783, 975
BstKTI GATC 3 cut(s) 190, 263, 403
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 3 cut(s) 187, 260, 400
BstMWI GCNNNNNNNGC 2 cut(s) 421, 1055
BstNI CCWGG 4 cut(s) 258, 265, 859, 926
BstSCI CCNGG 4 cut(s) 256, 263, 857, 924
BstSFI CTRYAG 3 cut(s) 138, 346, 432
BstUI CGCG 2 cut(s) 300, 781
BstX2I RGATCY 1 cut(s) 260
BstXI CCANNNNNNTGG 1 cut(s) 264
BstYI RGATCY 1 cut(s) 260
BtgI CCRYGG 3 cut(s) 298, 1000, 1026
BtsCI GGATG 4 cut(s) 84, 628, 875, 1036
BtsIMutI CAGTG 2 cut(s) 565, 777
CfoI GCGC 2 cut(s) 783, 975
Cfr13I GGNCC 3 cut(s) 103, 718, 806
Cfr42I CCGCGG 1 cut(s) 301
CseI GACGC 1 cut(s) 1047
Csp6I GTAC 4 cut(s) 35, 196, 331, 359
CviAII CATG 7 cut(s) 38, 129, 177, 443, 532, 1001, 1027
CviJI RGCY 9 cut(s) 250, 295, 351, 424, 682, 695, 745, 849, 868
CviKI_1 RGCY 9 cut(s) 250, 295, 351, 424, 682, 695, 745, 849, 868
CviQI GTAC 4 cut(s) 35, 196, 331, 359
DdeI CTNAG 4 cut(s) 411, 588, 725, 735
DpnI GATC 3 cut(s) 189, 262, 402
DpnII GATC 3 cut(s) 187, 260, 400
DraI TTTAAA 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco130I CCWWGG 3 cut(s) 389, 1000, 1026
Eco47I GGWCC 3 cut(s) 103, 718, 806
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 879
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 599
EcoRII CCWGG 4 cut(s) 256, 263, 857, 924
EcoT14I CCWWGG 3 cut(s) 389, 1000, 1026
ErhI CCWWGG 3 cut(s) 389, 1000, 1026
FaeI CATG 7 cut(s) 41, 132, 180, 446, 535, 1004, 1030
FaqI GGGAC 1 cut(s) 819
FatI CATG 7 cut(s) 37, 128, 176, 442, 531, 1000, 1026
FauI CCCGC 1 cut(s) 293
FauNDI CATATG 1 cut(s) 355
FbaI TGATCA 1 cut(s) 187
FokI GGATG 4 cut(s) 71, 635, 862, 1043
FspBI CTAG 3 cut(s) 30, 510, 830
GlaI GCGC 2 cut(s) 782, 974
GsuI CTGGAG 2 cut(s) 240, 841
HapII CCGG 1 cut(s) 950
HgaI GACGC 1 cut(s) 1047
HhaI GCGC 2 cut(s) 783, 975
Hin1I GRCGYC 1 cut(s) 1058
Hin1II CATG 7 cut(s) 41, 132, 180, 446, 535, 1004, 1030
Hin6I GCGC 2 cut(s) 781, 973
HinP1I GCGC 2 cut(s) 781, 973
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 6 cut(s) 5, 11, 535, 656, 872, 1034
HpaII CCGG 1 cut(s) 950
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 4 cut(s) 35, 106, 648, 997
Hpy188I TCNGA 5 cut(s) 10, 16, 187, 192, 271
Hpy188III TCNNGA 6 cut(s) 419, 596, 607, 710, 879, 1062
Hpy8I GTNNAC 4 cut(s) 35, 106, 648, 997
HpyAV CCTTC 4 cut(s) 291, 438, 465, 521
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 842
HpyF10VI GCNNNNNNNGC 2 cut(s) 421, 1055
HpyF3I CTNAG 4 cut(s) 411, 588, 725, 735
HpySE526I ACGT 1 cut(s) 842
Hsp92I GRCGYC 1 cut(s) 1058
Hsp92II CATG 7 cut(s) 41, 132, 180, 446, 535, 1004, 1030
HspAI GCGC 2 cut(s) 781, 973
Ksp22I TGATCA 1 cut(s) 187
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 3 cut(s) 187, 260, 400
LmnI GCTCC 3 cut(s) 300, 343, 952
LweI GCATC 1 cut(s) 424
MaeI CTAG 3 cut(s) 30, 510, 830
MaeII ACGT 1 cut(s) 842
MaeIII GTNAC 1 cut(s) 1021
MalI GATC 3 cut(s) 189, 262, 402
MboI GATC 3 cut(s) 187, 260, 400
MboII GAAGA 5 cut(s) 406, 751, 790, 904, 953
MfeI CAATTG 2 cut(s) 98, 363
MflI RGATCY 1 cut(s) 260
MluCI AATT 9 cut(s) 75, 98, 363, 565, 599, 793, 811, 907, 944
MlyI GAGTC 4 cut(s) 544, 665, 881, 1028
MmeI TCCRAC 1 cut(s) 745
MnlI CCTC 6 cut(s) 63, 470, 597, 613, 709, 795
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 8 cut(s) 291, 471, 552, 639, 705, 749, 906, 917
MslI CAYNNNNRTG 1 cut(s) 42
MspA1I CMGCKG 2 cut(s) 239, 300
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 4 cut(s) 258, 265, 859, 926
MunI CAATTG 2 cut(s) 98, 363
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 4 cut(s) 258, 265, 859, 926
MvnI CGCG 2 cut(s) 300, 781
MwoI GCNNNNNNNGC 2 cut(s) 421, 1055
NcoI CCATGG 2 cut(s) 1000, 1026
NdeI CATATG 1 cut(s) 355
NdeII GATC 3 cut(s) 187, 260, 400
NlaIII CATG 7 cut(s) 41, 132, 180, 446, 535, 1004, 1030
NlaIV GGNNCC 3 cut(s) 262, 666, 807
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 2 cut(s) 5, 11
PflFI GACNNNGTC 1 cut(s) 716
PfoI TCCNGGA 2 cut(s) 256, 857
PleI GAGTC 4 cut(s) 543, 664, 880, 1028
PpsI GAGTC 4 cut(s) 543, 664, 880, 1028
PpuMI RGGWCCY 1 cut(s) 806
PsiI TTATAA 1 cut(s) 759
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 4 cut(s) 256, 263, 857, 924
PspGI CCWGG 4 cut(s) 256, 263, 857, 924
PspN4I GGNNCC 3 cut(s) 262, 666, 807
PspPI GGNCC 3 cut(s) 103, 718, 806
PspPPI RGGWCCY 1 cut(s) 806
PsuI RGATCY 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 716
RsaI GTAC 4 cut(s) 36, 197, 332, 360
RsaNI GTAC 4 cut(s) 35, 196, 331, 359
RseI CAYNNNNRTG 1 cut(s) 42
SacII CCGCGG 1 cut(s) 301
SaqAI TTAA 8 cut(s) 291, 471, 552, 639, 705, 749, 906, 917
Sau3AI GATC 3 cut(s) 187, 260, 400
Sau96I GGNCC 3 cut(s) 103, 718, 806
ScaI AGTACT 1 cut(s) 197
SchI GAGTC 4 cut(s) 544, 665, 881, 1028
ScrFI CCNGG 4 cut(s) 258, 265, 859, 926
SfaNI GCATC 1 cut(s) 424
SfcI CTRYAG 3 cut(s) 138, 346, 432
Sfr303I CCGCGG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 301
SinI GGWCC 3 cut(s) 103, 718, 806
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 2 cut(s) 419, 869
SmoI CTYRAG 2 cut(s) 419, 869
Sse9I AATT 9 cut(s) 75, 98, 363, 565, 599, 793, 811, 907, 944
SsiI CCGC 4 cut(s) 119, 239, 298, 300
SspMI CTAG 3 cut(s) 30, 510, 830
StyD4I CCNGG 4 cut(s) 256, 263, 857, 924
StyI CCWWGG 3 cut(s) 389, 1000, 1026
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 845
TaqI TCGA 1 cut(s) 156
TasI AATT 9 cut(s) 75, 98, 363, 565, 599, 793, 811, 907, 944
TatI WGTACW 2 cut(s) 34, 195
TfiI GAWTC 2 cut(s) 5, 11
Tru1I TTAA 8 cut(s) 291, 471, 552, 639, 705, 749, 906, 917
Tru9I TTAA 8 cut(s) 291, 471, 552, 639, 705, 749, 906, 917
TscAI CASTG 2 cut(s) 565, 777
TspDTI ATGAA 2 cut(s) 165, 592
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 2 cut(s) 565, 777
Tth111I GACNNNGTC 1 cut(s) 716
VpaK11BI GGWCC 3 cut(s) 103, 718, 806
XagI CCTNNNNNAGG 1 cut(s) 213
XapI RAATTY 2 cut(s) 565, 599
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 3 cut(s) 30, 510, 830
ZrmI AGTACT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.